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Citations for Bio-Rad :
9001 - 9050 of 9194 citations for Glutathione Fluorescent 384 Well Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cytokines were assayed through using Bio-Plex Pro-Mouse Group I Cytokine 23-plex assay kit (M60009RDPD, Bio-Rad, Hercules, California, USA) and the Bio-Plex® 200 System ...
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) synthesis was performed using 1 μg of total RNA with the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The genes encoding for the zonula occludens-1 (Zo-1) ...
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bioRxiv - Microbiology 2023Quote: ... modified from previously published primers/probe (44)) was performed using the BioRad iTaq Universal Probes One-Step Kit (BioRad, Cat. No. 1725140). Thermocycling was performed on a BioRad CFX Connect Real-Time PCR Detection System (BioRad ...
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bioRxiv - Plant Biology 2023Quote: ... The 1st strand cDNA was synthesised from 1 µg of RNA for each sample using iScript™ cDNA Synthesis Kit (Bio-Rad). This cDNA was then used as the template in qRT-PCR reaction mixture after 10 times dilution ...
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bioRxiv - Physiology 2023Quote: ... The cDNA was obtained from equal amounts of total RNA by reverse transcription using an iScriptTM cDNA Synthesis Kit (Bio-Rad, CA). Gene expression of 96 fibrosis-associated genes were evaluated using a PCR array kit (RT² Profiler™ PCR Array Rabbit Fibrosis PANZ-120Z ...
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bioRxiv - Biochemistry 2023Quote: ... The determination of casein concentration by the Lowry method was performed using the DC™ protein assay kit purchased from Bio-Rad Laboratories (California ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized with 1 µg of total RNA by using the iScript cDNA Synthesis Kit (Bio-Rad, Cat# 1708891). cDNA was diluted 20 times and real-time quantitative PCR was performed by using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were developed using the SuperSignal West Pico Chemiluminescent Substrate kit (Fisher #PI34578) and visualized on a ChemiDoc™ Touch Gel Imaging System (BioRad #1708370).
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bioRxiv - Bioengineering 2023Quote: ... We then added 80 μL of X1 streptavidin phycoerythrin SA-PE (Bio-Plex Pro Reagent Kit III #171304090M, BioRad, Hercules, CA, USA) and incubated it for another 20 minutes ...
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bioRxiv - Physiology 2023Quote: ... One hundred nanograms of RNA was used as input to synthesize cDNA using the iScript cDNA synthesis kit (BioRad, Hercules, CA, USA). cDNA templates were used for quantitative real-time PCR ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 4°C at 20,160 x g for 10 minutes and supernatants were collected for protein measurement using the DC Protein Assay Kit (Bio-Rad, 5000111). Equal amounts of protein were loaded to run the gels (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Heart homogenates were analyzed for cytokine and chemokine levels using a Bio-Plex Pro Mouse Cytokine 23-plex Assay kit (Bio-Rad, #m60009rdpd) and ProcartaPlex mouse IFN-α/IFN-β 2-plex (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Once ready for testing we quantified the amount of mitochondria from each isolation using Bio-Rad Protein Assay Kit (Bio-Rad, #5000001), plated 3µg/50µl/well onto a 96 well plate with all animals having 6-8 technical replicates (101085-004 ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified with the RC DC Protein assay Kit II (cat. No. 5000122; Bio-Rad Labortaories Inc, California, USA). After protein concentration assessment ...
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bioRxiv - Pathology 2023Quote: ... Two μg of total RNA from each sample were used for cDNA synthesis using the iScript Advanced cDNA Synthesis kit (Bio-Rad, CA).
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bioRxiv - Zoology 2024Quote: ... and 100 ng of RNA were used for cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA), resulting in a final concentration of 5 ng/μl for each sample.
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bioRxiv - Neuroscience 2024Quote: ... The signals were detected using an enhanced chemiluminescent (ECL) plus reagent kit (Share-Bio, Cat. SB-WB012L, China) and viewed with a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Bioengineering 2024Quote: Four samples of total RNA were collected for each treatment condition using the Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... the fluorescence derived from the incorporation of SybrGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoAdvanced Universal SYBR Green Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Immunology 2023Quote: ... First-strand cDNA was synthesized from 200 ng of total RNA in a 20 μl reaction using the iScript™ cDNA Synthesis Kit (Cat#: 1708891, Bio-Rad). A SYBR green real-time PCR kit (Cat# 001752A ...
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bioRxiv - Microbiology 2024Quote: qRT-PCR analysis was performed on three major selected genes and validated as follows: The first cDNA strand was prepared using the iScript™ cDNA synthesis kit (Bio-Rad). qRT-PCR was performed using the SsoFast™ EvaGreen® supermix kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... CD45-APC and Bu1-PerCP-Cy5.5 were generated by conjugation of the antibody with the respective Lynx Rapid conjugation kit (Bio-Rad, Feldkirchen, Germany). Samples were analyzed on a FACS Canto II and FlowJo 10.9 software (BD ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using the iScript™ cDNA Synthesis Kit according to the manufacturer’s instructions (Cat#1708890, Bio-Rad Laboratories, Hercules, CA). Quantitative real-time PCR was conducted using SYBR Green probes and measured using the QuantStudio using the following PCR settings ...
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Plant Biology 2024Quote: ... The transcripts of defense marker genes were quantified by using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD, #1725150) with 40 cycles of 1 s at 95°C and 30 s at 60°C.
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bioRxiv - Developmental Biology 2021Quote: ... Real-Time reactions were performed in triplicates using KAPA SYBR FAST qPCR Kit (2X) on QuantStudio 5 Real-time PCR system (Bio-Rad, CA, USA) following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from the cerebellum using the Aurum™ Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Hercules, CA, USA). Reverse transcription was performed using the iScript™ cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Genomics 2019Quote: ... Extracted RNA was then transcribed to first-strand complementary DNA (cDNA) using the iScript Select cDNA synthesis kit (Bio-Rad laboratories, CA, USA) with its proprietary random primer mix ...
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bioRxiv - Immunology 2021Quote: ... DNA contamination in the treated RNA samples was assessed by performing a qPCR cycling with tilapia elongation factor 1α (EF-1α) primers using No-RT master mix (absence of reverse transcriptase enzyme provided in iScript™ Reverse Transcription kit, Bio-Rad, USA). The cDNA synthesis (20 µL reactions ...
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bioRxiv - Synthetic Biology 2019Quote: ... Gels were either silver-stained using SilverQuest™ Silver Staining Kit (Novex) according to the protocol of the manufacturer or Coomassie stained using Bio-Safe™ Coomassie Stain (Bio-Rad Laboratories). For densitometric determination of SDS-PAGE gel bands ...
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bioRxiv - Neuroscience 2019Quote: ... Instructions were followed as per the Bio-Rad Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Hemel Hempsted, UK, Cat# 7326830) and our previously optimised protocol (Pervolaraki et al. ...
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bioRxiv - Microbiology 2019Quote: One hundred microlitres of recombinant ZIKV NS1 antigen (ensuring at an optimal protein concentration range of 0.5-5.0mg/ml) were coupled to 100μg of lyophilized HRPO mix using the LYNX Rapid HPR Conjugation kit (Bio-Rad Laboratories Ltd, Watford, UK) in accordance with the manufacturer’s instructions Once conjugated the product was diluted 1 in 10 in HRP Stabilising Buffer (ClinTech ...
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bioRxiv - Immunology 2019Quote: ... cDNAs were synthesized from 1 μg total RNAs and qPCR were performed by using the iScript™ cDNA Synthesis Kit (BioRad, the Netherlands) and iQ™ SYBR Green Supermix (BioRad ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA synthesis and qRT-PCR was performed as described earlier (Neogy et al., 2019) using iScript cDNA synthesis kit and iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, India). Rice UBQ5-normalized ΔΔCt was used to calculate log2 fold change ...
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bioRxiv - Plant Biology 2020Quote: ... and the quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the iScript™ One-Step RT-PCR Kit (Bio-Rad®), marking with SYBR® Green (Bio-Rad®) ...
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bioRxiv - Microbiology 2020Quote: ... MNV or HAV RNA recovered by the ISO 15216-1 and PGM-MB or HAV antibody methods was quantified using the One-Step dd-RT-PCR Advanced Kit for Probes (Bio-Rad Laboratories, Ltd.) according to manufacturer’s instructions and as described previously (23 ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μg total RNA was reverse transcribed into cDNA in a reaction volume of 20 μl using the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad, 1725038). Real-time PCR reactions were performed on the resulting cDNA using the CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... 500 ng of total RNA were used in cDNA synthesis with iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, USA) while 700 ng of total RNA from the AT2 callus in LD and LL were used as an input ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the DC protein assay according to standard protocols provided by the kit manufacturer (Bio-Rad, Hercules, CA). Equal volumes of soluble protein supernatants were transferred to new tubes and subjected to in solution digestion ...
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bioRxiv - Cell Biology 2022Quote: ... The total amount of protein isolated from the weighed tissues was determined by using a protein assay kit (Bio-Rad, Hercules, CA, USA). Hydroxyproline content data are expressed as μg of collagen per mg of total lung protein (μg/mg).
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred into clean tubes and the protein concentration was determined using the Bradford protein assay kit (Bio-Rad Laboratories, USA). The protein extracts (20 μg ...
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bioRxiv - Biochemistry 2021Quote: Reverse transcription (RT) was carried out with 500 ng of total RNA using the iScript™ cDNA Synthesis kit (Bio-Rad, Hercules, CA). qRT–PCR was carried out with the iQ™SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... and up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 172-5038). Quantitative real time-PCR was performed using Go-Taq qPCR Master Mix (Promega ...
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bioRxiv - Genetics 2022Quote: ... and cDNA was synthesized by with iScript™ cDNA synthesis kit according to the directions of the manufacturer (Bio-Rad, Hercules, CA, USA). Quantitative PCR was performed using Power SYBR™ Green PCR Master Mix kit on a StepOne Plus real time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... One µg of DNase treated RNA was used to synthesize cDNA using iScriptTM Select cDNA synthesis kit (catalog#170-8896, Bio-Rad, Hercules, CA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Quality and quantity of retrieved RNA was assessed using the Experion automated electrophoresis system and a RNA StdSens Analysis kit (Bio-Rad Ltd., UK).
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bioRxiv - Microbiology 2020Quote: ... was used for quantification and all PCR reactions were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad Cat# 1864022). Primers used were previously described in (25) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Reverse transcription was performed with Onescript® cDNA synthesis kit (Abm) and qRT-PCR was performed using SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad) and a CFX96™ Real-Time System device (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Bio-Rad, Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...