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Citations for Bio-Rad :
8701 - 8750 of 9194 citations for Glutathione Fluorescent 384 Well Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: One ng of total RNA was used for single-tube reverse transcription (RT) and ddPCR using the One-Step RT-ddPCR Advanced kit (Bio-Rad). Droplets were generated and subsequently analyzed using the QX100 system (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of total RNAs was used to generate cDNA using the iScript cDNA synthesis kit (Bio-Rad, Richmond, CA, USA). Real-time PCR was subsequently performed using the iTaq Universal SYBER Green Supermix using the primers listed in Supplemental Table 1 on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche) ...
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bioRxiv - Immunology 2022Quote: ... was converted to cDNA with the C1000 Touch Thermal Cycler using the iScript cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad, 1708890), with the following modifications ...
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bioRxiv - Microbiology 2022Quote: ... about 25 ng of DNA-free total RNA for each lncRNA was reverse-transcribed using iTaq cDNA Synthesis Kit (Bio-Rad) and then analyzed with ABI 7500 Fast Real-time PCR System (Applied Biosystem ...
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bioRxiv - Plant Biology 2019Quote: ... RNA samples confirmed as free of genomic DNA were diluted to equivalent concentrations and subjected to cDNA synthesis with iScript cDNA Synthesis Kit according to the manufacturer’s instructions (BioRad, Hercules, CA). All cDNA synthesis reactions for a single time-point were performed simultaneously to decrease any inherent variability in the synthesis process ...
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bioRxiv - Genomics 2020Quote: ... Concentration and 260/280 ratios were quantified using a NanoDrop 1000 spectrophotometer before cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad). Primers were designed to span exon-exon boundaries wherever possible (Supplementary Table 2) ...
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bioRxiv - Genetics 2019Quote: ... Reverse transcription of 400ng of RNA into cDNA was completed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA) and the Veriti 96-well thermal cycler according to the manufacturer’s instructions (Applied Biosystems ...
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bioRxiv - Genomics 2019Quote: For a detailed description of tagmentation protocols and buffer formulations refer to the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Genomics 2019Quote: ... Cells were collected and resuspended in OMNI Tagmentation Mix containing ATAC Tagmentation Buffer and ATAC Tagmentation Enzyme that are parts of the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad). The OMNI Tagmentation Mix was buffered with 1X PBS supplemented with 0.1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... was performed using 300 ng RNA with the CFx Connect Real-Time system with the iTaq Universal SYBR Green One Step Kit (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and was reverse transcribed to cDNA using either qScript XLT cDNA SuperMix (Quantabio) or iScript™cDNA Synthesis Kit (Bio-Rad). Samples were run on an Applied Biosystems Viia7 Real-time PCR system ...
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bioRxiv - Physiology 2019Quote: ... 1 μg of RNA was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170-8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of total RNA from all samples were transcribed into cDNA using iScript cDNA Synthesis Kits (Bio-Rad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD, USA) for sequencing analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... 500ng of RNA from clinical samples were converted to cDNA using the iScript cDNA synthesis kit from Biorad (Catalog number: 1708891). Primers that overlapped intron-exon junction were designed using the NCI Primer-blast software and are listed in Supplementary Table 13 ...
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bioRxiv - Pathology 2020Quote: ... 1 μg of total RNA was used as a template for reverse transcription assay using the iScript cDNA Synthesis Kit (Bio-Rad); cDNA was used for detecting FAM210A and GAPDH expression ...
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bioRxiv - Cell Biology 2021Quote: ... The peroxidase-conjugated secondary antibody was incubated and reacted using ECL kits (Cytiva).The signal was detected using ChemiDoc XRS + (Bio-Rad). The membrane was stripped using stripping buffer (strong ...
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bioRxiv - Microbiology 2019Quote: ... First-strand cDNA for gene expression analysis using real-time qPCR was carried out using the iScript cDNA synthesis kit from Bio-Rad following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 250 ng of RNA was used as a template in each reaction with iTaq Universal One-Step RT-qPCR Kit (Bio-Rad) or Luna Universal One-Step RT-qPCR Kit (New England Biolabs) ...
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bioRxiv - Immunology 2020Quote: Reverse transcription: 200 ng of RNA was reverse transcribed using iScript Advanced cDNA Synthesis kit for RT-qPCR (1702537, Bio-Rad) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were resolved on SDS polyacrylamide gels and blotted onto PVDF membranes using Trans-Blot Turbo RTA Midi 0.45 μm LF PVDF Transfer Kit (Bio-Rad, 1704275). Transfer was run in Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... the membrane was then developed by incubation with chemiluminescence (Amersham ECL prime kit) and imaged with a ChemiDoc™ XRS+ System (BIORAD). The intensity of GFP bands were quantified with Image J software ...
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bioRxiv - Bioengineering 2021Quote: ... Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves: an iScript cDNA synthesis kit (Bio-Rad) was used to reverse transcribe total RNA into cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Austria).52 The luciferase activity data was normalised to protein content measured by DC Protein determination kit (Bio-Rad Laboratories, Inc., USA) and SCO effect was expressed as a fold-change over untreated cells ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK).
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bioRxiv - Molecular Biology 2020Quote: 500ng of genomic RNA were used for reverse transcription into cDNA using the iScript cDNA synthesis kit (Bio-Rad, #170-8891). cDNA was diluted 10X and 2uL was added for each qPCR reaction (iQ™ SYBR Green Supermix ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence substrate was then applied to the blot according to the manufacturer’s instructions (Bio-Rad Clarity™ Western ECL Substrate kit). The chemiluminescent signals were recorded using a Syngene G:BOX (Synoptics ...
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bioRxiv - Cancer Biology 2019Quote: Plasma activities of ALT and AST were assessed with the ALT and AST Reagent Kit (Biosystems Reagents) using a Benchmark Plus microplate spectrophotometer (Bio-Rad). Plasma concentration of non-sulfated bile acids were measured with the Bile Acid Assay Kit (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... ATAC Tagmentation Buffer and ATAC Tagmentation Enzyme (ATAC Tagmentation Buffer and Enzyme are both included in the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad)) ...
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bioRxiv - Genomics 2021Quote: For a detailed description of tagmentation protocols and buffer formulations refer to the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Genomics 2021Quote: ... One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD, 1708840). qPCR reactions for targeted genes were performed with the Luminaris HiGreen qPCR Master Mix (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... total 600 ng of RNA was used for cDNA synthesis by using Random hexamer oligonucleotide primer (iScript Select cDNA Synthesis Kit, BioRad, USA). Gene specific primers (Table S2 ...
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bioRxiv - Genomics 2020Quote: ... The concentrations of cytokines in plasma were measured using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit immunoassay run on the Bio-Plex 200 (BioRad), with reference to an 8 point standard curve.
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNAs were synthesized from 500 ng DNase-treated total RNA by RT with the iScript cDNA synthesis kit (Bio-Rad) in a total volume of 20 µL according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Serum was also collected for cytokine analyses which were performed by the University of Pennsylvania’s Human Immunology Core using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane by semi-dry transfer using the Trans-Blot turbo RTA Mini PVDF transfer kit (Bio-Rad) and the Trans-Blot turbo system ...
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bioRxiv - Physiology 2021Quote: ... was performed with the Bio-Rad iQ TM SYBR Green Supermix kit and data were analyzed with the CFX384 system (Bio-Rad). mRNA quantitation was done by normalizing the amount of RNA detected for the gene of interest ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the iTaq Univer SYBR Green 1-Step Kit (Bio-Rad) on a StepOnePlus Real-time PCR system (Applied BioSystems ...
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bioRxiv - Developmental Biology 2021Quote: ... eluted total RNA in 10 μl of Elution buffer and reverse transcribed RNA in 20 μl using the iScript cDNA synthesis kit (Bio-Rad). We incubated the samples for 5 min at 25°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA (1μg) of each postnatal stage was used for cDNA synthesis using a reverse transcription kit (iscript, Bio-Rad, USA). Real-time PCR amplification was performed using iTaq™ Universal SYBR® Green Supermix (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... The antigen-antibody complexes were visualized using appropriate HRP conjugated secondary antibodies and enhanced chemiluminescence kit (ECL; Amersham or Bio-Rad) and normalization was reported to β-actin ...
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bioRxiv - Physiology 2021Quote: ... 5 ul of each supernatant sample was used to quantify the total amount of protein with a BCA Protein Assay Kit whereas the rest was diluted in 4x Laemmli sample buffer (Bio-Rad) with 2-Mercaptoethanol (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... markers was analyzed by qPCR as previously described (Yanai et al., 2013; Yanai et al., 2015) using an iScript cDNA synthesis kit (Bio-Rad) and TaqMan Primer/probe sets for each gene ...
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bioRxiv - Immunology 2020Quote: ... with 18S (Hs99999901_s1) as housekeeping control were carried out on RNA reverse transcribed to cDNA with an iScript cDNA synthesis kit (Bio-Rad), using Taqman chemistry and gene-specific probes purchased from Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: Total RNAs were extracted from cell-sorter purified cells using NucleoSpin RNA kit with a DNase treatment (Macherey Nagel) and reverse transcribed with iScript™ Reverse Transcriptase mix (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription and real-time PCR (qPCR) reactions were carried out using iScript cDNA synthesis kit and iQ SYBR Green Supermix (Bio-Rad). qPCR was performed on a Bio-Rad CFX384 Touch™ Real-Time PCR Detection System using the following human primers (Forward and Reverse ...
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bioRxiv - Immunology 2021Quote: Plasma from each patient sample was analyzed using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit (Bio-Rad). Cytokine expression was measured using the Luminex xMAP® (Luminex Corporation ...