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Citations for Bio-Rad :
851 - 900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... equipped with a 5-mL Foresight Nuvia HPQ anion-exchange column prepacked with CHT ceramic hydroxyapatite XT mixed-mode chromatography media (Bio-Rad) or 25-mL column in-house packed with the same CHT media ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated on 10% or 4-20% CRITERION TGX gels under reducing conditions and transferred to Immun-Blot PVDF membranes (Bio-Rad). The membranes were blocked with 5% BSA or 5% nonfat milk in TBS-T (0.1% Tween20 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured using a Trypan Blue exclusion assay or TC20 automated cell counter (BioRad, Hercules, CA).
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bioRxiv - Cancer Biology 2024Quote: ... The viable cell density (VCD) and viability were measured using TC20 automated cell counter (Bio-Rad, Hercules, CA) or hemocytometer and trypan blue (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Ten microliters of each soluble or chromatin-bound nuclear fraction were loaded on 4-20% CRITERION TGX SDS-PAGE gels (Bio-Rad, #4561085).
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bioRxiv - Cancer Biology 2024Quote: ... denatured and spotted on a nitrocellulose membrane using the Bio-Dot SF (Bio-Rad, Hercules, CA, USA). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... using the transblot Turbo Transfer System (BioRad, 1704150). The membranes were blocked for 1 h with 5 % non-fat dry milk (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... in a BioRad IQ5 detection system (Bio-Rad, Portland, ME). The transcript level of the CoChR gene was normalized to the average levels of Gapdh and Rpl32 ...
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bioRxiv - Cancer Biology 2024Quote: ... SERPINH1 (Biorad #VMA00970), phospho-ERK1/2 (Cell Signaling Tech pThr202/pTyr204 ...
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bioRxiv - Cancer Biology 2024Quote: ... Laemmli Buffer (BioRad) and 10% β-mercaptoethanol were added to the samples and boiled for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in medium and counted at different incubation times (0, 24, 48, 72 and 96 h) on a TC 20 automated cell counter (Biorad). The experiments were conducted in triplicate for each timepoint ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were centrifuged for 15 min at 15,000 x g at 4°C and the supernatant was quantified with Quick Start Bradford 1X Dye Reagent (BioRad), using an albumin curve to determine the concentration of protein to be placed ...
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bioRxiv - Cancer Biology 2024Quote: ... and supernatants were quantified by the Lowry method (Bio-Rad). Assays were performed in triplicate using 25 μg of protein in lysis buffer supplemented with 25 μM of the fluorogenic substrate Ac-DEVD-afc ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were developed using Clarity Western ECL Blotting Substrate (BioRad) and imaged in the LICOR Odyssey Fc imaging system.
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were a-mouse or α-rabbit-HRP (1:5,000; Bio-Rad). Blots were developed using Clarity Western ECL Blotting Substrate (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant from the lysates was collected and protein concentration was determined using the Bradford Assay (Biorad, 5000006). For SDS-PAGE 20 mg of protein were mixed with 4x Laemmli buffer (240 mM Tris/HCL pH 6.8 ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 500 ng of total RNA using the BIORAD iScript cDNA cDNA synthesis kit (BIORAD, CAT#1708891), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Resolved protein extracts were transferred to PVDF membranes (BIORAD, 162-0177). Transfer success was assessed with Ponceau S solution ...
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bioRxiv - Cancer Biology 2024Quote: ... and blotted onto nitrocellulose membranes (Bio-Rad) following standard conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting complementary DNA (cDNA) was analyzed by quantitative PCR using iTaq Universal SYBR Green Supermix (Bio-Rad) in a ViiATM 7 Real-Time PCR System (Thermo-Fisher) ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... before imaging on ChemiDoc System (BioRad).
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bioRxiv - Cell Biology 2024Quote: ... All PCRs were performed using CFX96 Touch Real-Time Detection System thermocycler (BioRad). Cq values as reported by the thermocycler during the RTqPCR for the gene of interest were subtracted from those of ACT1 to obtain ΔCq ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed on the QuantStudio5 using Syber-Green (BioRad) and analysis was performed using the ΔΔCt method ...
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bioRxiv - Cell Biology 2024Quote: Samples were loaded onto 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) and transferred onto a 0.45 µm nitrocellulose membrane using the TransBlot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Blots were imaged with the ChemiDoc™ XRS+ Molecular Imager® (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed on the Applied Biosystems Step One Plus Real Time PCR System according to the manufacturer instructions using the iQ SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) kit ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR reaction mix was prepared on a hard-shell PCR plate (Bio-Rad, HSP9655), sealed with Microseal ‘B’ seals (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred onto a 0.45 µm nitrocellulose membrane using the TransBlot® Turbo™ Transfer System (Bio-Rad). Samples were blocked in 5% powdered milk + 1x TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was quantified using Bradford reagent (Bio-Rad, # 5000001), and equal amounts of protein were loaded in SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... with the SYBR Green PCR Mix (Bio-Rad, USA). The primers for detection are listed below ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) assays were conducted on the CFX96 real-time detection system (Bio-Rad, USA) with the SYBR Green PCR Mix (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... These samples were denatured at 100 °C in 4 × XT Sample Buffer (Bio-Rad, USA). N-glycosylation modification of proteins was assessed using the PNGase F Glycan Cleavage Kit (A39245 ...
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bioRxiv - Cancer Biology 2024Quote: ... on a ChemiDoc imaging system from BioRad. All antibodies and their concentrations used in this study can be found in Supplementary table 6.
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed for siRNA-mediated Brca1 and Brca2 depletion on qPCR CFX 96 Thermocycler (Bio-Rad) using gene-specific primers (Table EV 3) ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR reaction was analyzed on CFX opus Real-Time PCR device (Bio-Rad) and data were normalized to the expression levels of GAPDH as housekeeping gene.
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bioRxiv - Cancer Biology 2024Quote: ... and images were captured using a ChemiDoc XRS-System (Bio-Rad, USA). Signal intensities were normalized to GAPDH or Actin ...
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bioRxiv - Cancer Biology 2024Quote: ... protein was transferred to a PVDF membrane (Bio-Rad). Membranes were blocked in 5 % skim milk in TBST (50 mM Tris–HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... blots visualization was achieved using an enhanced chemiluminescence detection kit (Bio-Rad, USA) and images were captured using a ChemiDoc XRS-System (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... First-strand complementary DNA synthesis was performed with using iScript reverse transcription super-mix from Bio-Rad. Real-time qPCR was performed using SYBR green on Applied Biosystems QuantStudio 5 Real-Time PCR System ...
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Cell Biology 2024Quote: ... Extract concentrations were measured using Bradford assays (Bio-Rad), aliquoted ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using the Bradford assay (Bio-Rad, cat#5000006). An equal amount of total protein was resolved using SDS-PAGE gels and transferred to nitrocellulose membranes (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein concentration was determined by Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... on a Chemidoc™ MP Imaging System (Bio-Rad, USA). The primary antibodies used in this study were anti-GAPDH (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.2% Triton-X100 (Bio-rad, #1610407) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... The analysis was carried out using a CFX96 Touch thermal cycler (Bio-rad, USA). Primer sequences for qPCR are listed in Table 2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Chemiluminescent signals were recorded using the ChemiDoc Touch gel documentation system (Bio-Rad, USA). Densitometric analysis to estimate the relative abundance of proteins was performed using ImageJ (Plot Lanes tool).
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed real-time PCR with SsoAdvanced Universal SYBR Green Supermix (Biorad). The expression of target genes was normalized to GAPDH ...
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bioRxiv - Cancer Biology 2024Quote: ... was determined by using the QuantaSoft Analysis Pro (Bio-Rad) software.
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA was synthesized from 1μg RNA using iSCRIPT as per the manufacturer’s instructions (Biorad, Hercules, CA). qPCR analysis of samples was performed on the CFX96 Real-Time System (Biorad ...