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Citations for Bio-Rad :
8751 - 8800 of 9925 citations for Recombinant Rabbit IL17F Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... Equal quantities of protein (30 - 60 μg) were resolved on TGX-precast gels (Bio-Rad Laboratories Inc., Hercules, CA, USA) and subsequently transferred to PVDF blotting membranes (Millipore ...
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bioRxiv - Biophysics 2023Quote: ... Labeling efficiency was determined by measuring the absorbance of pyrene (χ346nm = 42,000 M-1cm-1) and protein concentration by 660 nm assay (Bio-Rad). An SDS-PAGE shift assay for isolating single-mSA bound ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then separated on 10-20% SDS-polyacrylamide gels and were visualized by staining with Coomassie blue (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... gels were briefly washed with water and proteins were transferred (25 V, 1.3 A, 7 min) onto nitrocellulose membranes (Bio-Rad) using Bio-Rad Trans Blot Turbo Transfer system ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and protein reduced by mixing 25μl of sample to 4.75 μl Laemmelli buffer (Bio-Rad #161-0737) and 0.25ul of beta-mercaptoethanol (Bio-Rad #161-0710 ...
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bioRxiv - Immunology 2023Quote: ... 15 to 30 µg of protein sample was processed for separation through SDS PAGE on 4–20% Criterion TGX precast gradient gels (BioRad). Gel run consisted of 15 min run at 70V followed by 45 min run at 100V ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were pelleted at 10,000 g for 10 min and the supernatants were used to determine the protein concentration using Bradford assay (Bio-Rad). After a preclearing step with Protein G Sepharose (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Separated protein bands were visualized using “Stain Free Gel” application mode on ChemiDoc MP Imaging System (Bio-Rad Laboratories, Inc.). Protein gel was transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked in 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... 10-50 μg protein was loaded on 10% acrylamide home-made or gradient (4-15% acrylamide) commercial (BIO-RAD, 4561086) Tris-Glycine-SDS gels along a protein ladder (PageRuler prestained protein ladder ...
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bioRxiv - Cancer Biology 2023Quote: ... 30-50 µg of nuclear proteins were loaded onto a 10% SDS-PAGE and transferred on nitrocellulose membrane using TransBlot apparatus (BioRad). Membranes were subsequently incubated with TBS Tween-20® 0.01% ...
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bioRxiv - Cancer Biology 2023Quote: ... SDS-PAGE resolved proteins were transferred onto a nitrocellulose membrane using the Trans-Blot Turbo™ transfer system (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Purified GST-KIN-EC-ter fusion protein from pooled gel filtration fractions was cross-linked to Affi-gel 15 beads (BioRad) in MOPS ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein per sample was resolved on a NuPage 4-12% Bis-Tris polyacrylamide mini gel (Bio-Rad) and transferred to a nitrocellulose membrane in Towbin transfer buffer containing 20% MeOH at 100V for 1 hour or 25V for 16 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates (35 µg protein) were run on 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membranes (Bio-Rad, 1620115). After blocking for 10 min with 5% nonfat dried milk in PBS with 0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed and blocked in 1x TBST with 5% bovine serum albumin before consecutive incubation with primary antibody (see Suppl. Table 3) and horseradish peroxidase-coupled protein A (Cymed) and detection using Clarity Western ECL Substrate (BioRad).
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bioRxiv - Immunology 2023Quote: ... Bead-bound proteins were then eluted in 2x Laemmli buffer (diluted from 4x Laemmli Sample Buffer, #161-0747, Bio-Rad) with betamercaptoethanol (#21985023 ...
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bioRxiv - Immunology 2023Quote: ... eluted proteins were run on 10% polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF) membranes (Trans-Blot Turbo Transfer System, BioRad). Membranes were blocked with 5% milk in Tris-buffered saline (TBS ...
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bioRxiv - Physiology 2023Quote: ... Proteins were denatured in Laemmli buffer and separated on 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were blocked with blocking buffer for 1 h in TBST containing 5% BSA or 5% milk ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrated protein was run on a Superdex Increase 200 (Cytivia) gel-filtration FPLC column on Biologic DuoFlow System (Biorad). The fraction corresponding to the peak corresponding to the molecular mass of the monomer protein (∼80kDa ...
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bioRxiv - Microbiology 2023Quote: ... SDS-PAGE was performed in 1X MES buffer and proteins were transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad, 1620112).
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bioRxiv - Cell Biology 2023Quote: ... Purity was confirmed by loading 1.125 µg of protein on a 12-well 12% Mini-Protean TGX gel (Bio-Rad), performing SDS-PAGE electrophoresis and fixing the protein with 40% methanol ...
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bioRxiv - Cell Biology 2023Quote: ... or poly-acrylamide gels and proteins were transferred overnight at 4°C on a nitrocellulose membrane using a liquid transfer system (BioRad). Non-specific sites were blocked with 5% non-fat dry milk in PBS 0.1% Tween 20 ...
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bioRxiv - Physiology 2023Quote: ... and left ventricle of wild-type C57 Bl6 mice was incubated with PC2 antibody (10 ug, Alomone laboratories) overnight complexed to magnetic protein G beads (Biorad). Following 3 rounds of washing ...
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bioRxiv - Immunology 2023Quote: ... between 20-30 mg protein was run on 10-20% SDS–PAGE gradient gels and transferred onto 0.22 μM PVDF membranes (1620177, Bio-Rad). After air drying to return to a hydrophobic state ...
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bioRxiv - Genetics 2023Quote: ... Quantitative analysis of protein levels was carried out using a GS-800 calibrated densitometer with ImageLab software (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg protein was loaded per lane on Mini-PROTEAN TGX Precast Gels (Bio-Rad Laboratories, Hercules, CA, USA). Following protein separation ...
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bioRxiv - Cell Biology 2023Quote: ... The appropriate HRP-conjugated secondary antibodies were used for the chemiluminescent detection of proteins with Clarity Max Western ECL Substrate (BioRad). Membranes were scanned with a ChemiDoc imaging system (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins on the gel were transferred to a polyvinylidene fluoride membrane using a Transblot SD semi-dry transfer system (BioRad) at 25V for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... wet blotting (at 200 mA per gel for 1 hour) was performed to transfer proteins onto 0.2 µm nitrocellulose membranes (Bio-Rad). Total protein staining was performed according to the manufacturer’s protocol (Revert™ Total Protein Stains kit ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured and proteins were separated by SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 490 nm using a microplate absorbance reader (Bio-Rad iMarkTM). See Fig ...
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bioRxiv - Physiology 2024Quote: ... Equivalent amounts of total protein were separated by SDS-PAGE on 4% to 15% Criterion TGX gels (Bio-Rad; 5671083). Megalin and cubilin were detected with anti-megalin antibody generously provided by Dr ...
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bioRxiv - Physiology 2024Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated in 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼45 min followed by 110 V for ∼65 min ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 µm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 μg of total protein lysate was loaded for each sample onto Mini-PROTEAN® Precast Gels (Bio-Rad, 4568123) with 4 × Laemmli buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were incubated in 5% BSA in tris-buffered saline (TBS)-Tween 0.1% (TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were separated by SDS-PAGE (30-50 μg total protein per lane) and transferred onto a 0.2 μm nitrocellulose membrane (Bio-Rad). Membranes were blocked with 5% skimmed milk for 30 min at RT and treated with primary antibodies (Supplementary Table S1 ...
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bioRxiv - Biophysics 2023Quote: ... Protein-DNA complexes were transferred from the gel to the PVDF membrane (Immun-Blot® PVDF Membrane, BIO-RAD, USA) using semi-dry blotting in 1x transfer buffer (48 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... protein signals were visualized by incubation of the membrane in ECL substrate (Clarity™ Western ECL Substrate, BIO-RAD, USA), and chemiluminescence was detected with Odyssey® Fc Imaging System (Li-COR ...
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bioRxiv - Biophysics 2023Quote: A total of 250 ng of purified proteins as labelled above each lane were loaded on TGX stain-free gels (BioRad) and run at constant 150V ...
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bioRxiv - Bioengineering 2023Quote: ... Protein transfer was performed on methanol-activated polyvinylidene fluoride (PVDF) membranes by Trans-Blot Turbo Transfer system (1704150; Bio-Rad). Post transfer the membranes were blocked in 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... The column was washed with 50 mL of wash buffer and then transferred onto a Fast protein liquid chromatography system (BioRad). The column was further washed until the UV trace was flat ...
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bioRxiv - Cell Biology 2023Quote: ... Detection of proteins was achieved following 5 min incubation with Enhanced Chemiluminescence (ECL) reagent and exposed using a ChemiDoc imaging system (BioRad). Loading control was performed by normalizing the target protein to the stain-free images of total protein ...
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bioRxiv - Microbiology 2023Quote: ... proteins were resolved by SDS-PAGE and transferred onto a PVDF membrane by using a transfer apparatus (Bio-Rad, USA). The membrane was probed with primary antibody against V2c (1:4000) ...
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bioRxiv - Cell Biology 2023Quote: ... and equal amounts of protein were loaded and run on 4-20% gradient SDS-PAGE gels (4561096; Bio-Rad Laboratories). Proteins were transferred from SDS-PAGE gels to nitrocellulose membranes using Trans-Blot Turbo (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...