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Citations for Bio-Rad :
9001 - 9050 of 9925 citations for Recombinant Rabbit IL17F Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred into a new tube and and the protein concentration was determined using a Bradford assay (Biorad #5000006) as described by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: ... The labelled proteins were further purified and separated from free Alexa Fluor dye using a Superdex 75 10/300 GL gel filtration column (Cytiva) using either an ÄKTA protein purification system (Cytiva) or NGC Chromatography System (Bio-Rad). The proteins were concentrated using the Amicon Ultra centrifugal filters (Merck ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were transferred to a new tube and an aliquot were used for protein quantification using the Bradford assay (Bio-Rad). A total of 10-20 μg of protein lysates were separated on 8% gels by SDS-PAGE and transferred to nitrocellulose membranes ...
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were sonicated and then resolved by SDS-PAGE on 10% gels along with Precision Plus All Blue protein prestained standards (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were transferred to nitrocellulose membranes in a Turbo blotter (Trans-Blot Turbo Blotting System, 170-4155, Bio-Rad Laboratories). The total protein amount was visualized using the stain-free image of the blot (Figure 1B) ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 10 µg protein was then separated by SDS/PAGE and transferred to Immuno-Blot PVDF membranes (Bio-Rad). Membranes were incubated in 5% non-fat milk for 1 hour at room temperature to block nonspecific binding ...
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bioRxiv - Genetics 2022Quote: ... The 13 µl of protein was run on a 10% SDS-PAGE gel and transferred to a PDVF membrane by semidry transfer (Bio-Rad) at 13V for 2 hours 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The Cy7.5 signal corresponding to persulfidated proteins was recorded at 800nm and the NBF-adducts signal was recorded at 488nm on a Chemidoc Imager (BioRad, France). Persulfidation levels were assessed by normalizing the Cy7.5 intensity to Alexa488 (NBF-adducts ...
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bioRxiv - Neuroscience 2022Quote: ... was performed for 2 h at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD).
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bioRxiv - Bioengineering 2022Quote: ... run at 120 V constant voltage (Pharmacia LKB, ECPS 3000/150) using a Hoefer gel system and Precision Plus Protein Kaleidoscope standards (Bio-Rad). Protein and peptide concentrations were determined by direct measurement on a Nanodrop 1000 UV-Vis spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated at 95 °C for 5 minutes prior to being resolved on a 4 – 15% Mini-PROTEAN TGX Precast Protein Gels (Bio-rad) and transferred onto a methanol activated Immobilon-P PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2022Quote: The source lysates of Brl1 APs 90 min post labeling were adjusted to 50 µL with a protein concentration of 4 µg/µL with lysis buffer as determined by the Bradford method (Bio-Rad). Samples were diluted with 200 µL guanidine chloride (7 M in 100 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2022Quote: ... 25µg of protein was loaded per lane into an SDS-PAGE gel (Bio-Rad Mini-Protean pre-cast gels 4-20%) and gels were run at 160V for approximately 90 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Protein bands visualized using film exposures in the linear range were imaged using a GS-900 calibrated imaging densitometer (Bio-Rad) and quantified using Image Lab Software (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... heated for 10 min at 70C then run out on a 4-12% Novex NuPAGE gel in 1X NuPAGE MOPS buffer with a protein ladder(Bio-Rad). The gel was transferred to a 0.2um PVDF membrane for 2 hours at 200mA at 4C ...
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bioRxiv - Molecular Biology 2020Quote: Samples were separated using 10-15% SDS-PAGE gels or 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad, 5678095). Gels were run at 100 V using 1 X SDS-PAGE Running Buffer [100 mL of 10 × 1 L Stock [30.2 g Tris Base (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: Equal amounts of protein from (30 μg, mouse cortex was used) were separated by electrophoresis in precast 4-12% Bis-Tris Gels (Bio-Rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Genomics 2021Quote: ... Proteins were subjected to electrophoresis through a 12% Glycine SDS-PAGE gel (Mini-PROTEAN TGX Stain-Free Precast gel, Bio-Rad) and transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of protein were then resolved by SDS-PAGE on either 7.5% or 10% polyacrylamide gels (Bio-Rad, Hercules, CA). Separated proteins were transferred to PVDF Immobilon-FL membranes (Millipore ...
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bioRxiv - Biophysics 2021Quote: ... and eletro-transfer at 100V for 2h were performed to separate protein extracts and transfer them to PVDF membranes (Bio-Rad). Non-specific sites were blocked with 5% BSA in TBS 0.05% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins (20 μg equivalent) were then separated by 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; Bio-Rad), then transferred by using 0.22-μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini- PROTEAN® TGX Stain-Free™ Gels (BioRad; Cat#17000435).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 20 μg of protein was separated using a 3–8% Criterion™ XT tris-acetate or a 4–15% Criterion™ XT tris-glycine protein gels and then transferred to PVDF membrane with a Trans-Blot Turbo Transfert system (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Fifty microliters of homogenate were used to measure cytokine and chemokine protein abundance using a Bio-Plex Pro mouse cytokine 23-plex assay (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... we ran similar amounts of protein per lane (~10-20 μg, depending on target) on a 4-20% Criterion TGX stain-free gel (Bio-Rad) at 200 V for 50 min with the cassette surrounded by ice ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples with normalized protein content were loaded into each lane (20 μg/lane) and resolved by SDS-PAGE (Bio-Rad, 456809). Total protein contents were imaged with the Bio-Rad Chemidoc MP Imaging System ...
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bioRxiv - Immunology 2022Quote: ... boiled for 10 min and subjected to protein separation by SDS-PAGE in 4 – 20% Mini-PROTEAN TGX precast gels (Bio-Rad) before transferred to nitrocellulose membranes (Wako) ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of total protein in Laemmli Buffer were subjected to electrophoresis on precast polyacrylamide gels and transferred to PVDF membranes (Bio-Rad). Membranes were blocked and probed overnight with corresponding primary antibodies (MERTK ...
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bioRxiv - Cell Biology 2022Quote: Half of the produced supernatant (see above) was dosed by the Bradford method (Protein Assay Dye Reagent Concentrate, Biorad #500-0006) and digested with 1% trypsin (weight/weight ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips pH 5-8 for CDK4 (BioRad, Hercules, CA, USA) or pH 3–10 for CDK2 (Amersham Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein extracts were separated using SDS page gels with 4X Laemmli loading buffer and transferred onto nitrocellulose (BioRad Cat. No. 3500601) using a wet transfer apparatus and blocked using either LI-COR Intercept (TBS ...
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bioRxiv - Biochemistry 2022Quote: ... IP-ed protein and its interacting partners were eluted from the beads in 60 µL of XT buffer (1610791, Bio-Rad) through heating at 95 °C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... Both input and IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed by Western blot using specific antibodies.
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bioRxiv - Biochemistry 2022Quote: ... Input and MOV10/IgG IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed through Western blot using specific antibodies.
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bioRxiv - Biochemistry 2022Quote: ... heated at 95 °C for 5 min and resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad). Proteins on the gel were transferred to the PVDF membrane ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Cell Biology 2022Quote: ... and the fractioned proteins were subsequently transferred electrophoretically onto polyvinylidene fluoride membranes via the Trans-Blot Turbo Transfer System (Bio-Rad). These membranes were blocked in 6% nonfat milk for 1.5 h at room temperature and then probed with antibodies against survivin (cat ...
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bioRxiv - Cancer Biology 2019Quote: ... Reducing buffer was added to the samples and the proteins were analyzed by SDS-Mini Protean TGX gels (Bio-Rad, CA) and transferred to Immobilon-P PVDF Membrane (Millipore Sigma ...
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bioRxiv - Immunology 2019Quote: ... and equal amounts of proteins were resolved by electrophoresis on 12% Tris-HCl gels (Mini-PROTEAN TGX gels; Bio-Rad, #4561043) and transferred onto polyvinylidene difluoride membranes (Trans-Blot Turbo Transfer Pack ...
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bioRxiv - Neuroscience 2019Quote: ... The concentration of IgG present after dialysis was determined using a modified Lowry assay (DC protein assay, BioRad, Hemel Hempstead, UK) and adjusted by dilution with Hartmann’s or by dialysis against a sucrose solution (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 150mM DTT was then added to each sample followed by the pelleting of membrane debris and undigested genomic DNA at 21000 x g for 5 min before the supernatant were separated on a Mini-PROTEAN TGX protein gel (Biorad, USA). Total protein was detected in gel by SYPRO Ruby Protein Gel Stain (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Final purification was performed by size-exclusion chromatography and the purity of the proteins was assessed by 4%-15% SDS-PAGE gels (Bio-Rad) under reducing conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... About 10 µg of the exosomal protein was separated on gradient (4-12%) SDS-PAGE gel and followed by transfer to nitrocellulose membrane (Bio-Rad). After blocking with 5% skim milk powder in Tris-buffered saline (TBS)-Tween for 60 min ...
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bioRxiv - Physiology 2019Quote: ... The mitochondrial pellet was resuspended in 25μL of isolation buffer and protein was quantitated using the Bradford assay (BioRad, #500-0006).
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bioRxiv - Plant Biology 2020Quote: Gel based LC MS was performed on protein samples using 12% polyacrylamide gel via Bio-Rad Mini-Protean III equipment (Bio-Rad) at 50 V for 15 min followed by 200 V until 1 cm mark below the stacking gel as described by Valledor and Weckwerth (2014b) ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts (15 µg) of protein lysates were heat denatured and separated on a 4–10% Mini-Protean Precast Gel (Bio-Rad), followed by transfer on a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... membrane proteins were blotted onto a polyvinylidene difluoride (PVDF) membrane (Trans-Blot® Turbo™ Mini PVDF Transfer Pack; Bio-Rad) with a Bio-Rad blotting system ...
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bioRxiv - Molecular Biology 2019Quote: ... with a molecular weight cut off of 10 kDa and the final concentration was estimated using the Bradford protein assay (5000006, Bio-Rad). The protein was also subjected to mass spectrometry to assess its purity and molecular weight ...