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Citations for Bio-Rad :
8651 - 8700 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated by protein electrophoresis at 150 V for 1h using 4-20% Mini-Protean TGX pre-cast gels (BioRad) and transferred by semi-dry technic onto Amersham Hybond-P PVDF membranes (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... Separated proteins were then electroblotted using a TransBlot Turbo system to TransBlot Turbo Mini-size PVDF membranes (Bio-Rad, 1704272). Membranes were subsequently blocked with 1x TBS containing 0.1% Tween 20 (1x TBST ...
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bioRxiv - Neuroscience 2019Quote: The amount of protein was prepared from homogenized tissues or tells and determined using the Bradford quantification assay (Bio-Rad). Protein extracts were separated by SDS-PAGE and electroblotted to PVDF membrane (Millipore ...
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bioRxiv - Physiology 2021Quote: ... About 30 μg of protein from the sample extracts were electrophoresed in 12% Mini-PROTEAN TGXTM precast gels (Bio-Rad) using the electrophoresis system mini-PROTEAN Tetra Cell (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... 20 µL of each sample was loaded and the proteins were resolved using 10% SDS-PAGE resolving gel followed by transfer to a polyvinylidene difluoride membrane (BioRad) at 100 mA for 12 h ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were analysed by Western Blot using custom-made SLR1 specific polyclonal antibody with Mini-Protean II system (Biorad).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes using either a Trans-Blot® Turbo™ Transfer System (7 minutes, 25V, BioRad) or an iBlot™ 2 Dry Blotting System (P0 method ...
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bioRxiv - Microbiology 2019Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad) per the manufacturer’s instructions and blocked with 100 mg/mL skim milk in 1x TBS/Tween 20 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using the Mini-PROTEAN® electrophoresis unit (BioRad, Hercules, CA) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay ...
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bioRxiv - Microbiology 2021Quote: ... NC-membrane (pore size 0.45 mm) was used for protein transfer using Bio-Rad Mini PROTEAN blotting system (Bio-Rad). Proteins were transferred for 1 h at 300 mA in transfer buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified and the same amount of protein was loaded to the wells in a 4-15% SDS-PAGE gel (BioRad). Protein was transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... depending on the proteins analysed) and transferred on 0.2 μm nitrocellulose membranes (Trans-Blot® TurboTM Transfert Pack, Bio-Rad). Equal total proteins loads were confirmed by Ponceau red (0.1% Ponceau ...
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bioRxiv - Cell Biology 2020Quote: Purified ORB proteins were concentrated in a stacking gel using SDS-PAGE stained with Coomassie Brilliant Blue R-250 (Biorad). Proteins in the excised gel band were subjected to in-gel digestion as follows ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Immunodetected proteins were identified using the enhanced chemiluminescence system (Clarity Western Blotting ECL Substrate, Bio-Rad Laboratories Ltd, Watford, UK) or Odyssey imaging system (LI-COR) ...
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bioRxiv - Cell Biology 2022Quote: ... as well as respective inputs (20 µl of extracts corresponding to 50 µg of total proteins) were separated using SDS PAGE electrophoresis and transferred onto nitrocellulose membrane by electro-blotting (SemiDry, BioRad). Western blots were probed with rabbit anti-GFP (1:5000 ...
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Total of 15-30µg protein were separated on a 4-12% Bis-Tris SDS PAGE and transferred using BioRad Turbo Blot (1704156, BioRad) onto PVDF membrane ...
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bioRxiv - Genetics 2022Quote: ... Proteins were separated by SDS-PAGE with 2-mercaptoethanol as a reducing agent using criterion sized 4-15% gradient tris-glycine for broad range of molecular weights greater than 30 kDa or 16.5% tris-tricine for high resolution of smaller proteins (Bio-Rad). Proteins were transferred to nitrocellulose membranes and probed with antibodies [commercially obtained except for ATF6 [63] ...
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bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
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bioRxiv - Biochemistry 2022Quote: Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... Aliquots of the lysates containing 10 mg of protein were denatured in Laemmli buffer and β-mercaptoethanol (Bio-Rad, USA) at 95°C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration for each condition was measured directly from 1 µL aliquot of the sample using NanoDrop (record A280nm measurements and use the exact same protein sample buffer as blank) and the Bradford assay (Biorad). After incubation for different amounts of time (1 hour after dialysis or dilution to allow droplet maturation ...
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bioRxiv - Physiology 2022Quote: ... Lysates were boiled and 20-60μg of protein was loaded onto Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). SDS-PAGE gels ran for 50 minutes at 125 V ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The proteins were then transferred to a nitrocellulose membrane on a Trans-Blot® Turbo (Bio-Rad; Hercules, CA, USA). The membrane was blocked in a PBS solution containing 5% non-fat milk and 0.1% Triton X-100 for 1 h with orbital agitation ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was separated by SDS-PAGE using 12% acrylamide Mini-protean TGX precast gels (Bio-Rad, USA). Gels were stained with Coomassie (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of protein was run per sample on 4–15% Mini-Protean TGX stain-free precast gels (Bio-Rad) at 150 V ...
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bioRxiv - Genetics 2022Quote: ... 60 μg of protein was separated on 10% SDS/PAGE (Mini-PROTEAN TGX Gels,12%,10-well, 4561043, BIO-RAD) and transferred onto nitrocellulose membrane (#1620115 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein samples were loaded on 10%-12% polyacrylamide gels and transferred onto 0.45 µm pore-size nitrocellulose membranes (Bio-Rad) using the Turboblot (BioRad) ...
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bioRxiv - Biophysics 2022Quote: Binding studies of various mRBD mutants with ACE2-hFc neutralizing antibody were carried out using the ProteOn XPR36 Protein Interaction Array V.3.1 from Bio-Rad as described previously (35) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and proteins were transferred to a PVDF membrane by using semi-dry Trans-Blot Turbo Transfer System (BioRad Laboratories, USA). Then the membranes were transferred into blocking solution (10% BSA or skimmed milk powder in PBS with 0.1% Tween20) ...
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bioRxiv - Biochemistry 2022Quote: ... Precast stain-free gradient gels for tryptophan fluorescence (4568084) as well as Precision Plus Protein Standards (1610393) were purchased from Biorad. Pyruvate kinase (P7768) ...
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bioRxiv - Molecular Biology 2022Quote: ... The total protein were separated via 8% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF, Bio-Rad, Cat no. #1620177) membrane using transfer buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were eluted by heating the beads at 95°C for 5 min in Laemmli Sample Buffer (1610747, Bio-Rad) with 50 mM DTT followed by magnetic separation ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were resolved by SDS-PAGE according to standard protocol and transferred onto 0.45μm nitrocellulose membranes (Bio-Rad 1620115) and blotted with the primary and secondary antibodies as described.
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bioRxiv - Cell Biology 2022Quote: ... 15-35 µg protein were loaded and separated by SDS-PAGE in 4–20% Mini-Protean TGX Precast gels (BioRad). Gels were transferred to PVDF membranes using the iBlot system (Thermo) ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein pellet was resuspended in 50 μL 8 M urea in 50 mM ammonium bicarbonate and the protein concentration determined using a Bradford assay (BioRad). Proteins were reduced using 10 mM dithiothreitol for 30 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 750 ng of wtXLF and each tandem dimer construct were loaded and run on a 4-15% Mini-PROTEAN TGX Precast Protein Gel (BioRad). tdXLF constructs where the KBM of subunit 1 was replaced with additional linker sequence (lane 4 tdXLFΔKBM/WT and lane 6 tdXLFΔKBM/ΔKBM ...
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bioRxiv - Neuroscience 2021Quote: ... Between 3 and 10 μg of total protein were loaded on to a 4-15% gradient TGX gel (Bio-Rad) and transferred on to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracted from tobacco or kiwifruit were separated on 12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad) using a highly efficient wet protein transfer system (eBlot L1 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% bromphenol blue) for 15min at 50°C and 5μg of protein were separated on a 4-20% SDS PAGE (Biorad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Microbiology 2020Quote: ... After electrophoresis the separated proteins were electroblotted onto a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) with a standard protocol for seven minutes ...
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bioRxiv - Developmental Biology 2021Quote: Protein samples were loaded on SDS-PAGE gel and then were transferred to Nitrocellulose membrane (Bio-Rad, Cat. Nr. 1620112). After blocking for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated on a 10% SDS-polyacrylamide gel and then transferred to a nitrocellulose membrane (BioRad, Boston, MA, USA). The membrane was incubated with the appropriate primary antibody and then incubated with a species-appropriate secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... and adjusted before isolation of protein aggregates as well as verified by running reduced samples on Criterion XT Precast Gel 4-12% Bis-Tris (BioRad) and staining with Coomassie Brilliant Blue R-250 staining solution (BioRad) ...
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bioRxiv - Biophysics 2020Quote: ACE2-hFc and CR3022 neutralizing antibody binding studies with the various RBD derivatives purified from different expression platforms were carried out using the ProteOn XPR36 Protein Interaction Array V.3.1 from Bio-Rad. Activation of the GLM sensor chip was performed by reaction with EDC and sulfo-NHS (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... The protein from the 5% of beads was eluted by boiling the beads in Laemmli sample buffer supplemented with BME (BioRad) and 2 mM biotin at 90°C for 10 min ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... 5 µg of protein was loaded per well on a 10% TGX 10-well 30µl/well gel (456-1033, Bio-Rad) for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... the gels were either stained with One-Protein Blue gel stain (#21003-1L, Bioticum) or transferred onto PVDF membranes for immunodetection using TurboBlot system (Biorad).
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bioRxiv - Plant Biology 2021Quote: ... 20 μg total protein was separated on 10% SDS-PAGE gels and blotted onto Immun-Blot PVDF membrane (Bio-Rad). LSD1-GFP ...