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Citations for Bio-Rad :
8601 - 8650 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 μg protein was denatured at 95 °C for 5 min and loaded on a 4-20% precast polyacrylamide gel (BioRad, 4568094) for gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... cleared supernatant was transferred to the new clean Eppendorf tubes and total protein concentration was measured using Bradford assay by Bio-Rad Protein Assay kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... About 15 μg of protein samples from lysates were boiled at 95°C for 5 min in Laemmli sample buffer (Bio-Rad) and resolved in 4-15% gradient SDS-PAGE gels (Mini-Protean TGX ...
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bioRxiv - Immunology 2023Quote: ... 1min activation of the gel and imaging of total protein on the blot was performed by a Gel Doc EZ Imager (Bio-Rad). Blots were blocked in Intercept blocking buffer (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein lysates were run on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and transferred to a nitrocellulose membrane (Bio-Rad) using a wet tank blotting system ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins (20–100μg as optimal for each antibody) for each sample was loaded into 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (BioRad; #4568084) for electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Protein (10 ug) from each sample was treated with ß-mercaptoethanol and subsequently electrophoresed on precast 4%–10% gradient gels (Biorad), as previously described (Alcantara et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Thermal stability of the protein samples was measured in a CFX96 Dx Real-Time qPCR instrument (Bio-Rad, Hercules, CA, USA); program ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...
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bioRxiv - Biochemistry 2022Quote: NKX2-5 homeodomain purity was evaluated through SDS-PAGE using 15 well Mini-PROTEAN TGX Precast Protein Gels® (Bio-Rad). Samples were prepared with 4X loading buffer containing β-mercaptoethanol (BME ...
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bioRxiv - Cell Biology 2023Quote: ... for protein extracted from cultured cells or brain sections and micro bicinchoninic acid (microBCA) for protein extracted from EVs according to the manufacturer’s instructions (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... and the samples were boiled for 5 minutes before being loaded on the gel (4-15% Criterion TGX Precast Midi Protein Gel, Bio-Rad). Proteins were separated by SDS-PAGE and blotted into a polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... and Precision Plus Protein Unstained Standards (#1610363) was resolved by SDS-PAGE using precast 4-20% MiniProtean TGX Stain Free Gels (Bio-Rad). The blot in Fig ...
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bioRxiv - Microbiology 2022Quote: ... and proteins were analysed either by western blot or by silver staining after electrophoretic separation on polyacrylamide SDS-PAGE gel (Bio-Rad 4–20% Criterion™ TGX Stain-Free™ Protein Gel ...
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bioRxiv - Bioengineering 2023Quote: ... Approximately 5 x 108 vesicles and purified HaloTag standard curve were loaded and run on a 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) at 150 V for 90 min or 50V for 10 min followed by 100V for approximately 70 min ...
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bioRxiv - Microbiology 2023Quote: ... equivalent total protein quantities from membrane samples were resolved by SDS-PAGE using Any kDTM polyacrylamide gels (Bio-Rad, Hercules, CA). Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 25 µg or 50 µg of normalized protein for LapA-HA or MapA-HA tagged strains respectively was mixed with 4x Laemmli Sample Buffer (Bio-Rad) and 2-mercaptoethanol to a 1x working dilution and samples were boiled for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... targeting SARS-CoV-2 gRNA and N protein sgRNA were used for the quantification by RT-qPCR using iTaq Universal SYBER Green Supermix (Bio-Rad) and normalised to β-actin using 2-ΔΔCT.
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bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of protein extracts were run at 100V for 1 hr on 10% pre-cast SDS-PAGE gels (Bio-Rad), then electro-transferred to 0.2 μm nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration of the supernatant was measured using the detergent compatible (DC Lowry) protein assay kit according to the manufacturer′s directions (Bio-Rad). For each sample ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µl of input and 5 µl of IP samples were loaded and protein samples were separated on 4-20% Tris-glycine polyacrylamide gels (Bio-Rad) at 170 V for 1 hour in 1X Tris/glycine/SDS running buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody was washed away with PBS-T and stained proteins were detected by chemiluminescence (Clarity western ECL, Bio-rad). The western blot signal was acquired using Amersham Image Quant8000.
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bioRxiv - Neuroscience 2023Quote: Seven DIV slice cultures were transfected with enhanced yellow fluorescent protein (eYFP) by particle-mediated gene transfer using a Helios Gene Gun (Bio-Rad), as described (Alonso et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad) and then transferred to the 0.2 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodisc formation was initiated by adding washed Bio-Beads (200 mg dry weight per 1 mL of the protein mixture; Bio-Rad), and the mixture was rotated for two hours at 4 °C ...
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bioRxiv - Microbiology 2023Quote: Dilution series containing equal amounts of protein were prepared and separated on 12% Mini-PROTEAN TGX gels (BioRad, Hercules, CA, USA) by SDS-PAGE and then transferred onto 0.2 µM polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: ... 35 µg of lysate was loaded on 10% SDS-PAGE after quantifying the protein concentration using Bradford assay kit (Bio-Rad). After transferring bands to nitrocellulose membrane Ponceau S staining was performed for checking equal loading of protein on SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ug of protein from cortical samples and 45ug of muscle samples were diluted in 4x Laemmli sample buffer (Bio-Rad). P3 brain samples were already directly resuspended in 4x Laemmli sample buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and the proteins were transferred to nitrocellulose membranes using a Turbo-Blot semidry transfer apparatus from Bio-Rad (Hercules, CA, USA). Immunoblotting was performed by incubating the membranes with 5% milk or bovine serum albumin for 60 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... different amounts of protein lysate were first loaded onto 12.5% SDS-PAGE test gels and proteins separated using the BioRad SDS-PAGE system (Biorad, Hercules, CA). Gels were run at 150 V for 50 min. ...
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bioRxiv - Biophysics 2023Quote: ... samples were processed using a Bio-Rad NGC Quest 10 Plus fast protein liquid chromatography (FPLC) apparatus with a 5 mL EconoFit Nuvia IMAC affinity column (Bio-Rad). To purify protein from the soluble fraction ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 20-30 μg of protein was resolved on 8% polyacrylamide gels and transferred onto nitrocellulose membranes using semi-dry Trans-Blot TurboTM (Bio-Rad). Following antibody incubation (anti-GFP ...
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bioRxiv - Biochemistry 2023Quote: ... Bio-Safe™ Coomassie stain and Precision Plus Protein™ dual color standard were obtained from Bio-Rad (Hercules, CA, USA). Gamborg’s B-5 Medium was acquired from Life Technologies (Carlsbad ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein was denatured at 95°C for 5 min in 1x Laemmli buffer containing 5% β-mercaptoethanol and separated in 4-15% mini-PROTEAN TGX Precast Protein Gels (Biorad) by electrophoresis and blotted onto Nitrocellulose membrane (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were subjected to electrophoresis under reducing conditions on 4–15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and subsequently transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... Samples were heated at 95 °C for 5 minutes to denature and reduce the proteins and were then separated by electrophoresis through 4-15% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad) with Tris/Glycine/SDS running buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was separated by non-reducing SDS-PAGE (6% acrylamide gel) with Precision Plus Protein Dual Color Standards (Bio-Rad) and transferred to nitrocellulose membrane overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 µg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...
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bioRxiv - Cell Biology 2023Quote: ... The denatured protein samples (20 µg) were then separated on 4-20 % SDS-polyacrylamide gels using a Tris-glycine running buffer (Bio-Rad) and electroblotted onto nitrocellulose membranes (0.2 µm pore size ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Mini TGX gels (10%, Bio-Rad, Hercules, USA) according to standard protocols and transferred to nitrocellulose membranes (0.2 μm ...
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 655 nm using a microplate absorbance reader (Bio-Rad Model 680). See Table S4 for raw Lowry assay data.
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 550 nm using a microplate absorbance reader (Bio-Rad Model 680). See Table S5 for raw BCA assay data.
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bioRxiv - Plant Biology 2023Quote: ... The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF) membrane (Bio-Rad). All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche ...
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bioRxiv - Neuroscience 2023Quote: Cell cultures and animal tissues: 15 μg of protein extracts were loaded on 10% pre-cast gels (Bio-Rad Cat.# 4568034) and run for 1hr at 100V ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 µg protein were loaded on 12% SDS-PAGE gels and separated with a Mini protean III system (Bio-Rad). The proteins were transferred onto nitrocellulose membranes by electroblotting adopting standard protocols ...