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Citations for Bio-Rad :
8451 - 8500 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Barcoded samples were then pooled and aliquoted into individual ChIP reactions with Protein A/G magnetic beads (BioRad; 161- 4013/23) coupled with the desired antibodies (5 ug each of H3K27me3 {Millipore 07-449} ...
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bioRxiv - Cell Biology 2020Quote: ... Fluorescently tagged/labeled expressed protein was detected both before and after SDS-PAGE using a Chemidoc MP imaging system (Bio-Rad, Laboratories Pty ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Native NiNTA lysis buffer (50mM NaH2PO4, 300mM NaCl, pH 8, 10mM imidazole) and protein concentration was measured via Bradford assay (Bio-Rad). 500-800μg of protein were incubated with NiNTA beads at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were loaded to each lane on a 4-20% gradient SDS/PAGE gel and transferred to nitrocellulose membranes (Bio-Rad). Signals were developed with HRP-conjugated secondary antibodies and SuperSignal West Pico Chemiluminescent Substrate (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... using a peristaltic pump and then eluted with a 0-250mM imidazole gradient using a NGC fast protein liquid chromatography (FPLC) system (Bio-Rad). The eluted samples were subject to immediate concentration with concomitant buffer exchange with fresh PBS to remove imidazole using Amicon centrifugal filters (Merck) ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty micrograms of total protein from each sample were loaded on 8% SDS-PAGE and transferred to PVDF membranes (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of protein per sample were boiled for 5 min and size separated by electrophoresis in polyacrylamide gels (Bio-Rad). Proteins were transferred over-night at 4 °C to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: The total proteins extracted from A549 cells were fractionated in a DS-Sepharose column with a BioLogic Duo-Flow system (Bio-Rad). About 40 mg of proteins in 40 ml of 10 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Immunology 2021Quote: The total proteins extracted from HFL1 cells were fractionated on DS-Sepharose columns with a BioLogic Duo-Flow system (Bio-Rad). About 40 mg of proteins in 40 ml of 10 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from lysates were separated by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE; Bio-Rad, Munich) and subsequently transferred to a nitrocellulose membrane by electroblotting ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates or lung homogenates in denaturing sample buffer (10-20 µg total protein) were separated by SDS-PAGE on 4-15% Mini Protean TGX gels (Bio-Rad) and transferred electrophoretically to a 0.45 µm nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of the resulting fractions were run on a 4-20% Mini-Protean TGX stain-free precast protein gel (Bio-Rad) at 200V for 30 min and stained by Imperial protein stain (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... 6µg of Round or Rod-shaped CCMVTT-VLPs were mixed with 2x mercaptoethanol and heated at 95°C for 3 minutes and then loaded into Any kD Mini-PROTEAN TGX precast protein gels (BIO-RAD). Gel was run for 35min at 180V ...
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Cell lysate proteins were resolved on a 4–12% gradient SDS-PAGE gel and transferred to Trans-Blot nitrocellulose membrane (Bio-Rad). Blots were incubated with polyclonal goat anti-mouse TLR2 Ab (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates of WT OSC were incubated with 1μg of Rabbit anti-Piwi polyclonal antibody (18) and 30 μl Surebeads Protein A Magnetic beads (Bio-Rad, 1614013). Immunoprecipitation was performed at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of eluted samples were electrophoresed for 8 min at 200 V on a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and protein was stained using Quick Blue Stain ...
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bioRxiv - Cell Biology 2021Quote: ... 30 µg of protein sample was loaded onto a 4 to 15% gradient sodium dodecyl sulfate (SDS)-polyacrylamid gel (Bio-Rad), followed by protein transfer to a polyvinylidene fluoride (PVDF ...
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bioRxiv - Genetics 2020Quote: ... Collected protein samples were boiled with 1× Laemmli buffer before SDS-PAGE procedure with Mini-PROTEAN TGX Precast Gel (Bio-Rad) in Tris/Glycine Buffer (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was loaded (10-50 µg per well, depending on protein of interest) and transferred using semi-dry turbo blotter (Bio-Rad). Blots were placed into 5% blocking solution containing primary antibodies and were incubated overnight at 4°C (Table 2) ...
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bioRxiv - Immunology 2021Quote: ... Tissue lysates were mixed with 2x Laemmli sample buffer and incubated at 100°C for 5 min and equal amounts of protein were loaded into a 4-15% polyacrylamide gel for SDS/PAGE and transferred onto a nitrocellulose membrane (Bio-Rad). Membranes were incubated with the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates containing approximately 40 μg of protein were boiled for 10 min prior to separation by 4-20% SDS-PAGE (BIO-RAD). After transferring at 90V to a nitrocellulose membrane for 90 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of proteins were separated on either 10% SDS/PAGE gels or 4-20% Mini-PROTEAN TGX Stain-Free Gels (#4568094, Bio-rad). Proteins were then transferred onto PVDF membranes and probed with the following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... adult testes or hippocampus or cell pellets were homogenized in RIPA buffer and the protein concentration was determined using a Bradford Assay kit (Bio-Rad). For blot analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of each sample was determined as described before and a total amount of 20 μg protein/sample was resolved on 4-20% gradient gels (Bio-Rad). Proteins were transferred onto nitrocellulose or PVDF membranes futher blocked with 5% non-fat dry milk-TBST ...
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bioRxiv - Immunology 2022Quote: ... Separated proteins were transferred to a PVDF membrane using the Trans-Blot Turbo RTA Transfer Kit (Bio-Rad Laboratories, Feldkirchen, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2022Quote: ... The total content of glycogen was normalized to content of soluble protein measured using the Bradford assay (Bio-Rad 500–0006) with BSA standard curves.
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bioRxiv - Molecular Biology 2022Quote: ... A calibration curve was generated by measuring the elution volumes of a series of standard proteins of known molecular mass (Bio-Rad). The molecular masses of pAgos complexes were calculated by interpolating their elution volume onto the calibration curve ...
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bioRxiv - Immunology 2022Quote: ... protein concentration was evaluated using a Bradford protein assay (adjusted to 1µg / µl in 1% sodium dodecyl sulphate) and additionally corrected by the evaluation of protein band intensities using a stain-free acrylamide solution (TGX FastCast, Bio-Rad). Prior to blotting bands were visualized by an imaging system (ChemiDoc ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were then transferred to a nitrocellulose membrane using a Trans-Blot Turbo Nitrocellulose Transfer Pack and Transfer System (Bio-Rad). The membrane was blocked for 1 h with 5% skim milk in PBS supplemented with 0.05% Tween 20 (PBS-T ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (BioRad, California, USA), which were blocked with 5% skim milk for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: Proteins were reduced with 1 mM TCEP for 1 h then passed through a 10DG desalting column (Bio-Rad, Hercules, CA) to remove the TCEP ...
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bioRxiv - Biochemistry 2022Quote: ... Equal quantities of protein were mixed with Laemmli sample buffer and loaded into a gradient SDS-PAGE gel (Ref 4561086 Bio-Rad). The proteins were transferred from the gel onto nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were heated in reducing Laemmli sample buffer at 95°C and resolved in SDS-PAGE protein gel and transferred to PVDF membrane (Bio-Rad). Membranes were blocked using 5% BSA (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µl HeLa lysate was preincubated with 1 µl of accessory protein for 10 min at room temperature in PCR plates (HSP9601, Bio-Rad). Reaction was started by adding 2 µl reaction mix ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was resolved on 10% SDS–polyacrylamide gel electrophoresis gels and transferred onto PVDF membranes (Bio-Rad, catalog no. 1704156) by Trans-Blot Turbo (Bio-rad) ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Membranes were visualized by chemiluminescence and under epi-blue to visualize total protein No-Stain labeling using a ChemiDoc MP imaging system (Bio-Rad). Densitometric analysis was performed using ImageLab software (Bio-Rad).
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bioRxiv - Genetics 2022Quote: ... and 25µg of protein was loaded in each well and resolved on 4-15% gradient Tris-Glycine gel (Bio-Rad - 4561086). Semi-dry transfer was done onto 0.2µm Nitrocellulose membrane as per Trans-BlotTurbo Kit (Bio-Rad - 1704270 ...
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bioRxiv - Genetics 2022Quote: ... or 25 µg (mouse tissues) of proteins were loaded per lane on Any kD or 4-20% Criterion TGX stain-free precast gels (Bio-Rad) and transferred to Immobilon-FL PVDF membrane (Millipore ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: ... the displayed S-trimer protein bands on SDS-PAGE gels were scanned and quantified by ChemiDoc MP imaging system (Bio-Rad) and ImageJ.
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...
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bioRxiv - Microbiology 2022Quote: ... All radioactive protein samples were separated by size on a 4-to-20% Mini-Protean TGX precast SDS-PAGE gel (Bio-Rad). The gel was then dried and imaged using a Typhoon phosphorimager (Molecular Dynamics GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and anti-mouse secondary antibody (Biorad; 1:10,000). GroEL was detected with anti-GroEL primary antibody (Pierce ...
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bioRxiv - Microbiology 2022Quote: ... β-lactamase activities were normalized relative to total protein concentration measured using Pierce Coomassie Plus Assay Kit (Bio-Rad, CA, USA) to obtain the enzyme activity in units pr ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Obtained soluble and insoluble fraction (pellet of cell debris) was analyzed by SDS-PAGE using 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and Precision Plus Protein Dual Color Standard (Bio-Rad).
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bioRxiv - Systems Biology 2022Quote: ... semidry electrotransfer of proteins to a PVDF membrane was carried out in a Trans-Blot Turbo Transfer System (Bio-Rad, USA). The membrane was incubated in blocking buffer (PBST ...