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Citations for Bio-Rad :
8501 - 8550 of 9474 citations for Recombinant Human ITGAV & ITGB6 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were denatured in Laemmli loading buffer as previously described,30 25 µg of protein was separated by 7.5% SDS-polyacrylamide acrylamide gel electrophoresis (SDS-PAGE) (Criterion, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2024Quote: ... 80-100 μg of protein were loaded onto 4–20% Mini-PROTEAN® TGX™ Precast Gel (Biorad, 4561093EDU, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting supernatant was transferred to a clean tube and protein concentration was determined using a Bradford assay (Bio-Rad). 200 μg tissue homogenate or 10 μg cell lysate was diluted in 50 μl CB lysis buffer and was used for the reaction with CatB Substrate (RR-amino-4-trifluoromethyl coumarin (AFC)).
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bioRxiv - Cell Biology 2024Quote: ... protein bands (IP or TCE) were transferred from polyacrylamide gel to a PVDF membrane using a TransBlot Turbo device (BioRad). PVDF membrane was blocked for 10 min with 8% blocking milk in Tris-buffered saline ...
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bioRxiv - Neuroscience 2024Quote: ... and the separated proteins were transferred onto PVDF membranes using a Trans-Blot Turbo semi-dry transfer system (Bio-Rad). Membranes were blocked using 3% BSA in TBS with 0.1% Tween20 (TBST) ...
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bioRxiv - Bioengineering 2024Quote: All relative protein quantities from western blotting were calculated by performing densitometry using ImageJ or Image Lab software (Bio-Rad) and normalizing to the average of the replicates of the PrgIWT samples ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by gel electrophoresis and transferred to nitrocellulose membranes using a semi-dry blotting system (Bio-Rad Laboratories). Membranes were blocked with 3% milk or 5 % BSA in TBST for 1 h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Protein signals were detected with Super ECL Detection Reagent (Yeasen, 36208ES60) in a ChemiDoc™ MP Imaging System (Bio-Rad). Native proteins were extracted using Column Tissue & Cell Protein Extraction Kit (Epizyme ...
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bioRxiv - Cell Biology 2024Quote: ... Equivalent micrograms of protein from each sample were diluted using 4X Laemmli buffer (Bio-Rad, Hercules, CA, USA, Cat # 1610747) supplemented with β-mercaptoethanol (Millipore-Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured in Laemli Buffer and boiled for 10 minutes then run on a 4-20% Criterion TGX precast midi protein gel (BioRad) and transferred to a PVDF membrane (Millipore Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... Tissue lysates containing equal amounts of total protein were mixed with 4x Laemmli Sample Buffer (Bio-Rad, Hercules, CA, USA) and incubated at 96 °C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD and pA-LS NP protein expression was analyzed using SDS-PAGE and subsequent Western blot on PVDF membrane (Biorad) using StrepMAB-Classic HRP 1:3000 (2-1509-001 ...
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bioRxiv - Immunology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane using the Mini Trans-Blot® Cell and Criterion™ Blotter (Bio-Rad). Proteins on the membrane were stained with Ponceau S buffer ...
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bioRxiv - Immunology 2024Quote: ... 7.5 µg of proteins were separated on polyacrylamide gel and then transferred on nitrocellulose membrane by the Trans-Blot dry system (Biorad). Membranes were blocked on milk (5% ...
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bioRxiv - Neuroscience 2024Quote: ... Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well (ThemoFisher, NP0321BOX) and transferred to polyvinylidene fluoride (PVDF) membranes (Biorad, 1704156EDU). PVDF membranes were blocked with 5% nonfat dried milk in Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto either a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (4568094, Bio-Rad) or 4– 20% Mini-PROTEAN® TGX™ Precast Protein Gel (4561094 ...
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bioRxiv - Biochemistry 2024Quote: Polar flagellins purified from bacterial growths of each mutant strain (2 µg) were separated by 12% SDS-PAGE as described previously [31] and stained with Coomassie Blue total protein stain (BioRad), according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... Protein purity and size were validated on an SDS-PAGE gel (Bio-Rad Mini-PROTEAN TGX and Mini-TETRA cell). Purified proteins were stored at –80°C.
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bioRxiv - Immunology 2024Quote: ... 70µg of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad, 4561095). Gel was transferred to a low fluorescence PVDF membrane (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... Thermo #26616) were run in pre-cast 4– 20% TGX stain-free denaturing protein gels (Biorad #4568093, #4568096, #5678094, #5678095) in a minigel system or criterion system (Biorad #1658005 ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated by SDS-PAGE 4 - 12 % gradient Bis-tris gel (Novex) followed by protein transfer to a PVDF membrane (BioRad) and antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein was boiled for 5 min at 95°C and then loaded on a 4-15% SDS gel (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... and the probed proteins were transferred to Bio-Rad polyvinylidene fluoride membranes using a semidry electroblotting transfer system (Bio-Rad) at 25 V for 2 h and 30 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred from gels to the PVDF membrane using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were incubated in 5% nonfat dried milk in 1X TBST (Tris-buffered saline ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
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bioRxiv - Microbiology 2024Quote: ... sonicated and their protein contents were analysed by electrophoresis on TGX Stain-free pre-cast SDS-polyacrylamide gel (Bio-Rad). Proteins were then transferred on PVDF membranes (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred from gels to nitrocellulose membranes for Western Blot analysis using the Turbo-Blot transfer system (Bio-Rad). Membranes were blocked using 1% w/v BLOTTO (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration of the cytosolic fraction from each sample was measured using the Bradford assay (Protein Assay Dye Reagent Concentrate; Bio-Rad). After normalization ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Cancer Biology 2021Quote: ... The same amounts of protein extracts were subjected to polyacrylamide gel electrophoresis using the 4% to 20% Mini-Protean TGX gel system (Bio-Rad), transferred to PVDF (0.45 μm pore size ...
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bioRxiv - Evolutionary Biology 2020Quote: Samples were heated at 95°C for 10 minutes before loading into 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and running at 150V for approximately 90 minutes at 4°C in 1X tris-gylcine running buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... Stain-Free gels were activated with UV light for 1 minute and imaged for total protein on gel using a ChemiDoc MP imager (Bio-Rad). Proteins were transferred to PVDF blotting membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of protein were loaded and separated by SDS-PAGE gel electrophoresis on 4%-20% Criterion gels (BioRad, Hercules, CA), and then transferred to PVDF membranes ...
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bioRxiv - Genetics 2021Quote: ... 150 μg of protein was then separated on Criterion Precast Gradient 4-15% SDS-PAGE gels (Bio-Rad, cat. number 5671083) and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... Aliquots of larval homogenate were removed prior heat-treatment to measure soluble protein using a Bradford assay (Bio-Rad 500-0006) with BSA standard curves.
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts (30 μg) from each protein sample were separated by SDS-PAGE and transferred to PVDF membranes (PVDF; Biorad, USA). The membranes were blocked with 10% (W/V ...
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bioRxiv - Molecular Biology 2021Quote: ... Ten to twenty micrograms of denatured protein was loaded onto a 4-20% TGX stain-free polyacrylamide gel (Bio-Rad, USA) or NuPAGE 3-8% Tris acetate gel and subsequently transferred onto pre-cut nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins on the gels were transferred onto PVDF membrane using BioRad Mini Trans-Blot system (BioRad, Mini Trans-Blot® Cell). The membranes were washed with pure water and blocked with 5% skimmed milk in 0.1% Tween-20/PBS (PBST ...
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bioRxiv - Evolutionary Biology 2021Quote: ... SDS PAGE was performed using 7.5% or 4-20% Mini-PROTEAN® TGX™ precast protein gels (BIO-RAD, 4561023/4561094) run in running buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... from Sigma was used to quantify triglyceride levels and protein levels were measured using the Quick Start™ Bradford 1X Dye Reagent (Cat. # 500-0205) from Biorad. This was followed by colorimetric estimation using TECAN Infinite M200 pro-multimode plate reader in 96-well format ...
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bioRxiv - Developmental Biology 2020Quote: ... and 50 µg of denatured proteins were run on a 12% acrylamide gel and transferred to a 0.2 µm nitrocellulose membrane (BioRad, Hercules, CA). We blocked membranes in 5% Milk in TBST (Tris Buffer Solution + 0.1% Tween ...
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bioRxiv - Cancer Biology 2022Quote: ... 15-25 μg of proteins were loaded onto 4-12% Mini-PROTEAN Tris-Tricine Precast Gels or Criterion Tris-Tricine Precast Gels (Bio-Rad), and electro-blotted on nitrocellulose membranes (GE Healthcare Life Science) ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (20 μg) were separated using an Any kD gel (SDS-PAGE) and transferred to a nitrocellulose membrane (BioRad, Temse, Belgium). Aspecific binding sites were blocked for 30 min using TBS + 1% casein (BioRad ...
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bioRxiv - Neuroscience 2021Quote: 10-40 µg of denatured protein extracts were separated by SDS-PAGE using 10% acrylamide gels or 4-15% Mini-Protean precast gels (Bio-Rad) for 2 h at 120 V and transferred onto methanol-activated PVDF membranes at 350 mA for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP was normalized to total protein bands per lane (visualized by a stain-free scan on a Bio-rad gel doc). Each experiment was repeated at least two times from biological replicates.
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bioRxiv - Molecular Biology 2020Quote: ... For western blotting, separated proteins were transferred onto a nitrocellulose membrane (Whatman, Dassel, Germany) using a Trans-Blot Turbo transfer system (Bio-Rad). The membrane was blocked and then incubated for 1 h with an His-tag-specific mouse mAb antibody (Proteintech ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were electrophoresed for 70 min at 80 V in a BioRad MINI-PROTEAN Tetra system (BioRad Laboratories, CA, USA), and the gel was stained with Coomassie Brilliant Blue R-250 (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of protein (typically 10 μg) were separated under reducing conditions on 10 or 12 % TGX-polyacrylamide gels (Bio-Rad) and transferred to Hybond-P membrane (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... Extracts were then incubated on ice for 10 min before protein concentration was calculated by Bradford assay (Bio-Rad, 500-0006). Extracts were then mixed with Laemmli buffer and boiled at 95°C for 5 minutes before loading on SDS-PAGE gels.