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Citations for Bio-Rad :
8301 - 8350 of 9474 citations for Recombinant Human ITGAV & ITGB6 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... thawed and centrifuged at full speed in an Eppendorf microcentrifuge for 15 minutes and protein content was assessed by Bradford assay (Biorad). Cell lysate equivalent to 1 mg protein collected 36 hours post-transfection was diluted to 500 μl in TGH buffer for each immunoprecipitation reaction and precleared with 10 μl sepharose-protein G beads (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... and 30 μg of total protein were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad). Correct protein loading was confirmed by Ponceau staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples from total or immunopurified ribosomes or aliquots from TCA precipitated sucrose gradient fractions were run in 1X Laemmli buffer on 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad) in 1X Tris/Glycine/SDS buffer (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled with SDS sample buffer and resolved on a 10% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein cellular lysates (20μg) were analysed by 4-15% gradient SDS-PAGE and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked with 5% skim milk in 1 x PBS (Invitrogen ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: For blotting the SDS/PAGE-separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semiwet transfer (Bio-Rad) and blocked for 1 h with PBS/2% milk.
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bioRxiv - Immunology 2021Quote: ... Luminex beads were coupled to the Spike protein as previously described (63) and added to a Bio-Plex plate (BioRad). Beads were washed with PBS 0.05% tween using a magnetic plate washer (MAG2x program ...
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bioRxiv - Immunology 2020Quote: ... first Protein A/G agarose was loaded on Micro Bio-Spin™ Chromatography Columns (Bio-Rad Laboratories, Hercules, CA, USA). After gravity flow ...
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bioRxiv - Microbiology 2021Quote: hCMP-mRBD protein kinetic binding studies to ACE2-hFc and CR3022 antibody were performed on a ProteOn XPR36 Protein Interaction Array V.3.1 (Bio-Rad). The GLM sensor chip was activated with sulfo-NHS and EDC (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and vasopressin) peptides were spotted onto a 0.2-μm polyvinylidene fluoride membrane (Immun-Blot PVDF Membrane for Protein Blotting, BIO-RAD). The membrane was air dried at room temperature and was washed for 10 min in 0.05 M Tris buffer (pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... gels were subjected to a western blot in which protein was transferred onto a 0.45 μm nitrocellulose membrane using a Mini Trans-Blot Cell (Bio-Rad), in transfer buffer in the presence of an ice block for 1 hour at a constant current of 255 mA ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 14,000 rcf for 20 min at 4°C and protein amounts in the supernatant determined using the Bradford assay (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Antibodies were affinity purified using either the peptides linked to SulfoLink beads (Pierce) or the Ascaris proteins (without GST) linked to a mix of Affigel-10/15 (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared in Licor protein sample loading buffer and run on TGX 7.5% and 4-20% Tris-glycine gels (Bio-Rad) and blotted onto a nitrocellulose membrane ...
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bioRxiv - Genetics 2020Quote: ... 10 µg of protein were loaded in each well of a gel (Bio-Rad # 567-1094, 567-1095, 456-1036). Blots were blocked in 5% milk/TBST for two hours at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in RIPA buffer and protein extracts from equal numbers of cells were resolved on a 12% SDS-PAGE gel (Biorad), followed by transfer onto Immun-Blot PVDF membranes (Biorad) ...
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bioRxiv - Bioengineering 2020Quote: ... after which proteins were transferred to polyvinylidene fluoride (PVDF) membranes (catalog no. 162-0177; Bio-Rad Laboratories; Hercules, CA, USA). Membranes were blocked with 5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... and equal protein amounts were boiled at 95°C for 10 minutes prior to being run on a gel (BioRad) at 120V ...
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bioRxiv - Cell Biology 2022Quote: ... 4-8 µL of samples and 3 µL of Precision Plus Protein Dual Color Standard (cat. no. 1610374, Bio-Rad) were run on 4-12% Bis-Tris Bolt gels (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... the amount of GFP-bound protein was assessed by loading final extract in sample buffer (without boiling) on a Mini-PROTEAN TGX Precast Gel (BioRad), visualised with LED488 illumination (G-box ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 μg of total protein was separated on a 4–20% SDS-PAGE gel (mini-PROTEAN TGX gels, Bio-Rad) along with PageRuler Prestained protein ladder (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots were performed with 4-20% Mini-PROTEAN(R) TGX(tm) Precast Protein Gels (Bio-rad, #4561095 or #4561096) and Trans-Blot ® Turbo™ Mini PVDF Transfer Packs (Bio-rad #1704156) ...
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bioRxiv - Bioengineering 2022Quote: ... and the nanocluster size was derived by eluting 20.0 μL of the sample with PBS buffer at a flow rate of 0.5 mL/min and calibrating the chromatograph with that of the protein standards (Bio-Rad). The hydrodynamic size and zeta potential of supraclusters were determined using a Zetasizer NANO ZS-90 instrument (Malvern) ...
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bioRxiv - Biochemistry 2022Quote: ... proteins in gels were transferred to a PVDF membrane using a Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) and blocked with 5% milk ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted samples and whole cell lysate controls were loaded and run on a 10% Mini-PROTEAN TGX protein gel (Biorad), visualized with AcquaStain (Bulldog Bio) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged at 14,000g for 15 min at 4°C and supernatant protein concentrations were determined by Bradford assay (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... proteins were transferred to nitrocellulose membrane from SDS- or native-PAGE gels using the Transblot Turbo semidry transfer apparatus (BioRad) at 25V for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were boiled with SDS-PAGE sample loading buffer (6X SDS) and 30 ug of protein was loaded to each well of a 10% Tris-glycine SDS-PAGE gel (BioRad). Proteins were transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (15 μg) were loaded on 4-15% 1 D polyacrylamide Mini-PROTEAN TGX Stain-Free gels (BioRad) and run in 1X Tris Glycine SDS buffer (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: Quantification of protein levels from western blot bands was done using the Evolution-Capt Edge software (Vilber) or ImageLab software (Biorad). The data presented are expressed as means ± SD ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Cell Biology 2022Quote: ... 30 and 36 hpi respectively on a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (4561083, Bio-Rad). Blotting was performed using the Trans-Blot Turbo Mini 0.2 μm Nitrocellulose Transfer Pack (1704158 ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad). Membranes were blocked in 10% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
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bioRxiv - Genetics 2022Quote: ... the antibodies were prepared by mixing 3 ug of antibody HNRNPK and IgG (as a negative control in a separate tube) with 650 ul of Dilution Buffer and 50 ul of Surebeads Protein G magnetic Beads (BioRad) and left in rotation for 30 minutes at 4 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... Ribosome fractions were resolved by SDS-polyacrylamide gel electrophoresis on 10% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad). Prior to loading ...
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bioRxiv - Physiology 2022Quote: ... the electrophoresed proteins were transferred from SDS-agarose gel to a nitrocellulose membrane (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with a semi-dry western blot system (Pierce Power Blotter ...
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bioRxiv - Neuroscience 2022Quote: 10 μL of tissue samples (25 μg of protein per well) were placed into each well of the Western blot gel (Biorad Criterion TGX Stain free 4-15% 18 wells ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts of total protein were loaded on a 4-20% gradient SDS-PAGE gel (Mini Protean TGX, Bio-Rad), then blotted onto nitrocellulose membranes and probed with anti-villin mouse monoclonal antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 20 μg of total proteins in tissue lysates were separated on electrophoresis using 4 - 12% gradient SDS-polyacrylamide gels (BioRad) and transferred to the PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were subsequently separated by electrophoresis on precast gels (4-20% mini protein TGX gels, Bio-Rad catalog #456-1094) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted protein concentration was estimated using a Bradford assay (Quick Start™ Bradford 1x Dye Reagent #5000205; Bio-Rad).
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bioRxiv - Plant Biology 2022Quote: ... approximately 60-70 μg of protein was loaded onto 7 cm pH 3-10 NL and pH 4-7 IPG strips (BioRad) and subjected to first-dimensional electrophoresis with increasing voltages from 50V to 2000V ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... detergents and undigested protein were removed using a Bio-Scale Mini Macro-Prep High S 1 mL cartridge (Bio-Rad) on an Agilent 1100 HPLC system at 1 mL/min ...
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bioRxiv - Plant Biology 2023Quote: ... An equal amount of protein bands were manually appointed per lane and adjusted in the Lane profile menu of the Image Lab software (BioRad). Band intensity was quantified relative to the unreacted control sample.
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bioRxiv - Neuroscience 2022Quote: ... Absorbance at 450 nm was measured in a plate reader (Thermo) and the total protein content of the samples was obtained by the Bradford assay (Biorad). As GDNF is not expressed in the mouse cortex ...
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bioRxiv - Microbiology 2023Quote: ... the Stain-Free visualization was enabled by activation of the gels for 45 seconds with UV-light before the proteins were transferred by Trans-Blot Turbo Transfer (Biorad) onto a nitrocellulose membrane (Trans-Blot Turbo RTA ...
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bioRxiv - Plant Biology 2023Quote: Protein concentration was measured via Bradford assay using a Quick Start™ Bradford 1x Dye Reagent (Biorad, Cat. No. 5000205) prior to protein content normalization ...