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Citations for Bio-Rad :
801 - 850 of 1380 citations for Recombinant Human Angiopoietin 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... after which 2 µL were used per qRT-PCR reaction mixture (iQ SYBR Green Supermix, BioRad). Total reaction volumes were 15 µL with 250 nM of each primer (Supplementary Table 3) ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred to a nitrocellulose membrane using the iBlot 2 Dry Blotting System (Bio-Rad) with pre-assembled dry transfer stacks ...
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bioRxiv - Microbiology 2022Quote: ... Commercial antibodies were used for β-tubulin (clone YL1/2, Bio-Rad, Watford, UK; 1:5000), β-actin (Abcam ab8227 ...
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bioRxiv - Biophysics 2022Quote: ... This protein-lipid-DDM suspension was then incubated with Bio-Beads SM-2 resin (Bio-Rad) to gradually remove the detergent (8 ...
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bioRxiv - Microbiology 2024Quote: ... the pellet was resuspended in 200 μL 4x Laemmli Sample Buffer with 10% 2-mercaptoethanol (BioRad). The samples were denatured by boiling for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μg of protein extracts were mixed with 4x Laemmli sample buffer with 2-mercaptoethanol (Biorad) and heated at 95°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Samples were purified and sequenced using a DNA Engine Tetrad 2 Peltier Thermal Cycler (BIO-RAD) and the ABI BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2024Quote: ... 2) Arm B was purified on an ENrich™ SEC 650 24 mL column (Bio-Rad), and 3 ...
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bioRxiv - Biophysics 2024Quote: ... at RT for 2 hours and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... the sample was incubated overnight with 500 mg SM-2 Bio-Beads (Bio-Rad, Hercules, California) under the same conditions.
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bioRxiv - Genetics 2024Quote: ... 4 mg/ml lyticase) and blended with 300 μl of 2% low-melt agarose (Bio-Rad). Then ...
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bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of extracts were resuspended in reducing (5.0 % 2-mercaptoethanol) sample buffer (Bio-Rad) and boiled at 100°C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: Wing discs (20 discs per lane) were lysed in 2× Laemmli Sample Buffer (Bio-Rad, #1610737) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Biophysics 2023Quote: ... detergent was removed after 4 h of incubation with Bio-Beads SM-2 (Bio-Rad, USA) pre-equilibrated with the reconstitution buffer ...
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bioRxiv - Cell Biology 2023Quote: The following reagents were used to prepare the PAA gels: 2% bis solution (Bio-Rad #1610142), 40% acrylamide (Bio-Rad #1610140) ...
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bioRxiv - Biophysics 2023Quote: ... 500 μL sample from step 2 was overlaid to a self-made SEC column (Bio-Rad Glass Econo-Column Chromatography Columns ...
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bioRxiv - Molecular Biology 2023Quote: ... Reconstitution of nanodiscs was achieved upon detergent adsorption by Bio-Beads SM-2 resin (Bio-Rad) which was added in three batches ...
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bioRxiv - Immunology 2023Quote: ... with 10 % 2-Mercaptoethanol and loaded onto 4-20% precast polyacrylamide gels (4561096, 5671095; Bio-Rad). Imaging was performed with enhanced chemiluminescent detection (34096 ...
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bioRxiv - Bioengineering 2024Quote: ... stained for >2 hours with Bio-Safe™ Coomassie Stain (#1610786, Bio-Rad Laboratories, Inc, USA), and de-stained in water for > 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... the mixture was electroporated in a precooled 2 mm Gene Pulser cuvette (Bio-Rad, Hurcules, USA) at 1.8kV ...
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bioRxiv - Biochemistry 2024Quote: ... smegmatis MC2155 at 2.5 kV in a 2 mm cuvette using a MicroPulser device (Bio-Rad). Liquid starter cultures were grown in 7H9 Middlebrook medium (7H9 Middlebrook base [HiMedia] ...
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bioRxiv - Systems Biology 2024Quote: ... and run on precasted gels (Criterion 12+2 well comb, 45 µl, Bio-Rad Laboratories Inc). The gel was run at (Criterion ...
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bioRxiv - Cancer Biology 2022Quote: ... Luminex analyses were performed using the Bio-Plex ProTM Human Cytokine 27-Plex Assay as well as the Bio-Plex Pro™ TGF-β Assay (both Bio-Rad Laboratories, Hercules, USA) according to the manufacturer’s protocols on the Bio-Plex® Reader 200 by using the Bio-Plex Manager™ software.
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bioRxiv - Cell Biology 2021Quote: Secreted IL-6 and TNFα cytokines were measured in an amnion and choriodecidua-conditioned medium using a Bio-Plex Pro™ Human Cytokine 27-plex Assay (Bio-Rad, Marnes-la-Coquette, France) as recommended by the manufacturer along with Luminex technology ...
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bioRxiv - Immunology 2022Quote: ... The levels of soluble immune mediators were measured in TA samples using a high-throughput microbeads array (Bio-Plex Pro™ Human Cytokine 27-plex Assay, Bio-Rad Laboratories, Hercules, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... All patients were HIV tested using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Microbiology 2019Quote: ... Relative fluorescence was measured at 5-minute intervals for 2 hours in a CFX96 system (Bio-Rad).
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bioRxiv - Plant Biology 2020Quote: ... containing 2 ml of anion exchange resin AGMP-1M (100-200 mesh, chloride form) (BIO-RAD, 1411841). The resin was first cleansed three times with 10 ml 18.2Ω water followed by 7 ml 0.5N HNO3 ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 μl of gene-specific primer pair and 5 μl iTaq Universal SYBR Green Supermix (Bio-Rad). The relative transcript abundance of each gene (normalized to rpl4 ...
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bioRxiv - Microbiology 2021Quote: ... fixed with methanol supplemented with 2% H2O2 and incubated with a biotinylated anti-RSV antibody (Bio-Rad) in PBS containing 1% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: Plates were incubated for 2-3 days at 30 °C and imaged using a ChemiDoc MP (BioRad). Individual colonies from the plates generated to enable high second stress survival quantitation were counted using in-house developed MATLAB scripts ...
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bioRxiv - Plant Biology 2021Quote: ... ramp rate set at 2 °C/sec] was performed in a thermal cycler (Bio-Rad C1000 Touch). Droplets were quantified in a QX200 Droplet Reader (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: Cells were mounted on a 2% agarose pad containing resuspension medium using a gene frame (Bio-Rad). Cells were concentrated by centrifugation (3300g for 30 s ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 hours at room temperature before signal development with Western ECL Substrate (Bio-Rad Laboratories, #1705061) and imaging under ChemiDoc XRS+ System (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2022Quote: ... 2 μl of diluted vRNP mixtures was loaded onto a 5% polyacrylamide native TBE gel (Bio-Rad) and run at 125 V for 80 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... boiled for 2 min and then analyzed on a 4–20 % SDS-PAGE gradient gels (Bio-Rad). The protein bands were then transferred to a nitrocellulose membrane ...
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bioRxiv - Immunology 2020Quote: ... and detergent removed by adsorption to polystyrene BioBeads SM-2 (3 mg; Bio-Rad; overnight, 4°C). Proteoliposomes were collected by ultracentrifugation (180,000 xg ...
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bioRxiv - Immunology 2019Quote: ... fluorescence images were acquired before and after a 2 min incubation after trypan blue (0.1%; Bio-Rad), which was added carefully to minimize sample movement ...
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bioRxiv - Cell Biology 2019Quote: ... RT-qPCR was performed on 2 μl of 1/25 diluted cDNA with a CFX-96 (Biorad) thermocycler ...
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bioRxiv - Immunology 2019Quote: ... IL-2 mRNA amounts were determined with a SYBR Green PCR master mix from Life Technologies (4344463) relative to mRNA for β-2 microglobulin on a DNA Engine Opticon II System (Bio-Rad) using the following oligonucleotides ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.009% H2O2) for 2 min and images were taken with a ChemiDoc Imaging System fitted (Bio-Rad). Densitometry was performed with Image Lab 5.0 software (Bio-Rad) ...
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bioRxiv - Genomics 2021Quote: ... RT-PCR amplicons were electrophoresed on 2 % agarose gels and imaged on a ChemiDoc MP (Bio-Rad). The resulting images were analyzed using ImageJ (v1.52a) ...
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bioRxiv - Microbiology 2020Quote: ... or 500 ng to 3 µg for integrative plasmids (MicroPulserTM electroporator Biorad in 2 mm cuvettes (Eurogentec) at 25 µF ...
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bioRxiv - Immunology 2021Quote: ... 2-ul of cDNA was added to qPCR reaction containing iQ SYBR green master mix (Bio-Rad) and 10-pmol of gene-specific primers ...
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bioRxiv - Microbiology 2022Quote: ... 400 Ω) was performed in a 2-mm cuvette using the Gene Pulser Xcell Electroporation System (BioRad). After electroporation ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 minutes at 72°C using the C1000 Touch Thermal Cycler (Bio-Rad, Los Angeles, CA). Primer sequences are listed in the Supplemental Methods.
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bioRxiv - Cell Biology 2022Quote: ... The detergent was then removed by four successive additions of Bio-Beads SM-2 Resin (Bio-Rad) over the course of ∼24 h at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was mixed with sample loading buffer supplemented with 1% v/v 2-mercaptoethanol (BioRad, Cat #1610710). Sample was not heated to prevent aggregation of the GPCR ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μL of the diluted samples were used as input in a ddPCR supermix (Bio-Rad, 1863023). Primers and probes ...