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Citations for Bio-Rad :
801 - 850 of 1200 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... Protein concentration values for each resulting lysate were determined using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) employing vendor provided protocols ...
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bioRxiv - Developmental Biology 2019Quote: ... Lysates were cleared at 16,000 g and protein concentration determined using the Bio-Rad Protein Assay Dye (Bio-Rad). Lysates were resolved by 8% SDS-PAGE ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... The protein lysate concentration was quantified using Pierce BCA Protein Assay Kit (ThermoScientific) and10μg of soluble protein lysates were separated on a 4-12% Bis-Tris gel (BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole cell lysates were separated through 7.5% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (Bio-Rad Laboratories, Inc.). Membranes were blocked with 5% milk powder in 0.1% Tween 20 in 1x Tris-Buffered Saline (TBST ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration of lysates was measured using the BIO-RAD DC protein assay according to the manufacturer’s instructions (Biorad). For precipitation experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein lysates were separated on 12% or 4%-15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad), transferred on a nitrocellulose membrane (BioRad ...
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bioRxiv - Immunology 2020Quote: ... The total amount of proteins in lysates was quantified by the DC™ Protein Assay (500-0116JA; Bio-Rad) and 2104 EnVision Multilabel Plate Readers (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: Total protein concentration of each cell lysate sample was determined by Bradford assay with Protein Assay Dye (Bio-Rad) on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Genomics 2021Quote: ... Protein concentrations of the lysates were determined using a Bio-Rad DC protein assay kit (Bio-Rad, catalog # 5000111), the lysates were denatured in the SDS-PAGE loading buffer at 100°C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... clarified lysate was separated by SDS-PAGE and transferred to nitrocellulose using Trans-blot Turbo Transfer System (Bio-Rad). Proteins were probed with antibodies against FLAG M2 (Sigma) ...
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bioRxiv - Genetics 2022Quote: ... Worm lysates or E3 ligase reaction mixtures were resolved on 4-20% stain-free SDS-PAGE gels (Bio-RAD) and transferred to Millipore Immobilon-P PVDF membranes ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentrations of culture medium and cell lysate were determined by the Bradford assay (Bio-Rad, Hercules, CA). Culture medium or cell lysate samples were resuspended in SDS sample buffer containing β-mercaptoethanol and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were separated by SDS-PAGE using 4-20% gradient gels (Mini-PROTEAN TGX Stain-Free gels, Biorad #4568096). Protein transfer and detection were performed as described above.
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bioRxiv - Cell Biology 2022Quote: ... Lysates were passed through a 26G syringe and protein was quantified by DC Protein Assay (Bio-Rad, cat #5000112). Lysates were diluted to equal volumes and 30 ug protein was loaded per well using the Novex WedgeWell Tris-Glycine Welcome Pack (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of lysates were loaded onto the SDS-polyacrylamide gel and subsequently transferred to a PVDF/0.2μm nitrocellulose membrane (Bio-Rad). Blots were incubated overnight with primary antibodies diluted at 1:1000 in 5% non-fat milk at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 15µg of protein lysate was loaded into a 4-20% polyacrylamide Criterion TGX stain-free gel (Bio-Rad, #5678095). Electrophoresis was performed using 60V for one hour followed by 125V until finished ...
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bioRxiv - Bioengineering 2023Quote: ... lysates were cleared by centrifugation and protein quantitation was performed using the BCA method (Pierce, 23225).15-30µg of lysate were separated using precast 7.5% or 10% SDS-PAGE (Bio-Rad) and then transferred onto PVDF membranes using the Transblot-turbo system (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: Fractionation of nuclear and cytoplasmic fractions from Drosophila lysates was conducted with the ReadyPrep Protein Extraction Kit (Bio-Rad). Seven whole flies per group were homogenized in cytoplasmic extraction buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf lysates were cleared by centrifugation at 10,000×g for 5 min and total protein normalized via Bradford Assay (Biorad). Extracts were prepared for SDS-PAGE with a 5x loading buffer containing 10% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Approximately 30 μg of ovarian lysate was combined with a 4x Laemmli Sample Buffer (Bio-Rad, Hercules, CA, 1610747) prepared with 2-Mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: Each lysate was separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). Immunoblotting was performed by using the following primary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... whole cell lysates were separated by SDS-PAGE and transferred to 0.45 um Immun-Blot PVDF membranes (BIO-RAD). Membranes were then blocked in 5% milk/TBST for 1 hour at room temperature and incubated with primary antibodies at 4 °C overnight ...
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bioRxiv - Immunology 2023Quote: The biotinylated ODN-bound proteins were precipitated with streptavidin beads from mitochondria lysates and resolved by PAGE (Bio-rad). The PAGE gels were stained using a silver staining kit (Pierce) ...
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bioRxiv - Biochemistry 2023Quote: ... CCT was immunoprecipitated from the lysate by adding 4 µg of a CCT5 antibody (BioRAD MCA2178, 1:100 IP) and incubating for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (50 µg) were loaded into 4–20% PROTEAN® TGX™ Precast Protein Gels (BioRad, Cat# 5678081). After overnight transfer to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2023Quote: Protein lysates were boiled in reducing sample buffer and resolved on 4-20% Criterion TGX polyacrylamide gels (Bio-Rad), then transferred to 0.2 µm nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of lysate was resolved via SDS-PAGE on a 4-20% gradient polyacrylamide gel (4561095; Bio-Rad) and transferred to a nitrocellulose membrane using the TransBlot Turbo mini-nitrocellulose kit (1704270 ...
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bioRxiv - Neuroscience 2023Quote: ... Equal protein lysate concentrations (40μg per well) were loaded on a 4%-20% precast Mini-Protean gradient TGX gel (Biorad). Proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-blot turbo transfer system (Bio-Rad) ...
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bioRxiv - Biophysics 2023Quote: ... Equal amounts of denatured lysates were loaded on a 12% SDS gel and ran with SDS running buffer (Biorad) for 90 minutes at 100 V ...
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bioRxiv - Cancer Biology 2023Quote: ... We loaded the lysates at equal protein amounts and concentrations on 4-15% precast TGX gels (Bio-Rad, USA) or 10% hand- poured SDS-PAGE gels ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were separated by SDS-PAGE and transferred to PVDF membranes using the Trans-blot Turbo Transfer System (BioRad). Membranes were blocked in Intercept PBS blocking buffer (LiCOR ...
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bioRxiv - Molecular Biology 2024Quote: Input lysates (5 μg of total protein) and entire elution products were combined with 4x Laemmli buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... The protein concentration of each lysate was analyzed using Bio-Rad protein assay reagent according to the manufacturer’s instructions (Biorad). Luciferase units obtained were normalized to the protein content of each well ...
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bioRxiv - Biochemistry 2024Quote: ... Samples of the total lysate and elution were analyzed side-by-side in CriterionTM T Precast gels (Bio-Rad). Gels were stained with Coomassie or further processed for Western blotting and immunodetection using anti-PINK1 (#BC100-494 ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Molecular Biology 2019Quote: ... the frozen tissues were first homogenized in PureZOL RNA isolation reagent (Bio-Rad) using MP FastPrep-24 (MP Biomedicals ...
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bioRxiv - Neuroscience 2024Quote: ... The total protein concentration of prepared tissue extracts was measured by Bio-Rad protein assay (Bio-Rad Laboratories GmbH ...
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bioRxiv - Immunology 2024Quote: ... frozen tissue was processed using Bio-Plex® Cell Lysis Kit (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate samples containing 4 μg of protein were fractionated using a 4–20% SDS-PAGE gel (Bio-Rad). Proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... whole cell lysates were separated on 8% sodium dodecyl sulfate (SDS) polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (BioRad). The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 µg of total protein lysate was loaded on to an TGX Stain Free FastCast Acrylamide gel (BioRad, cat# 1610185) and transferred using an iBlot2 gel transfer device (Thermofisher scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufactureŕs instruction and measured using the EonTM High Performance Microplate Spectrophotometer (BioTek ...
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bioRxiv - Molecular Biology 2021Quote: ... We denature the lysates and resolve using 10% (vol/vol) SDS/PAGE before blotting on PVDF membrane using a TransBlot Turbo (BioRad). Blots are incubated with antibodies against SOD1 (Sigma SAB5200083 ...
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bioRxiv - Molecular Biology 2020Quote: Whole-cell lysates were run on 10 or 15% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (1620097, Bio-Rad) by standard methods ...
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bioRxiv - Molecular Biology 2019Quote: ... SDS lysates from beads after co-IP were resolved on 8% SDS polyacrylamide gels and transferred to nitrocellulose membranes (BioRad). Membranes were blocked with 5% low-fat milk in TBS buffer supplemented with 0.1% Tween 20 (blocking buffer ...
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Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cellsbioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4– 15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h ...
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bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h and then incubated with primary overnight and secondary antibodies for 2 h ...
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bioRxiv - Biochemistry 2019Quote: Trypanosome whole cell protein lysates (2×106 cells per well) were separated using SDS-PAGE (10% gels) and transferred by semi-dry blotting (BioRad) for 45 min at 25 V to PVDF membranes ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...