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Citations for Bio-Rad :
701 - 750 of 1200 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... Total protein lysates were resolved on Tris glycin 4-15% acrylamide mini protein TGX stain free gels (BioRad). Proteins were transferred to nitrocellulose membranes using the trans-blot Turbo apparatus and transfer pack ...
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bioRxiv - Microbiology 2020Quote: ... The lysate with the beads was passed through a gravity flow chromatography column (Econo-Pac chromatography columns, Biorad). The column was washed with four column volumes of washing buffer (50 mmol L−1 NaH2PO4 ...
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bioRxiv - Microbiology 2020Quote: ... Total protein lysates were analyzed by SDS-PAGE using precast Tris-HCl 4-20% criterion gels (Bio-Rad). Primary polyclonal antibodies for Cfp29 were generated by GenScript USA Inc via immunization of rabbits with three peptides from the protein sequence ...
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bioRxiv - Cell Biology 2022Quote: Total protein concentration of cell or liver lysate was determined using the Lowry assay (Bio-Rad Laboratories, 5000112JA). Equal amounts of protein were loaded onto Tris-glycine sodium dodecyl sulfate-polyacrylamide gels ...
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bioRxiv - Cell Biology 2022Quote: The lysates were analyzed by 4-20% SDS-PAGE using Mini-PROTEAN TGXTM Precast Protein Gels (BioRad 4561095). Molecular mass markers were Precision Plus Protein All Blue Standards (BioRad 1610373 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were mixed with 4x Laemmli sample buffer and run on a 4–20% gradient gel (BioRad) and transferred to nitrocellulose filter (BioRad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Five μl of lysate was resolved on precast 4–20% Mini-Protean TGX Stain Free Gels (Bio-Rad) before being transferred to a polyvinylidene difluoride membrane by semi-dry transfer with a TransBlot Turbo (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... The protein lysates were centrifuged and the supernatants were subjected to SDS-PAGE (gradient 4-20%, Bio-Rad) and electroblotted to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates (45 ng) were prepared in equal volume with 2x Laemmli Sample Buffer (cat. #1610737, Bio-Rad) and loaded on 10% Mini-PROTEAN® TGX Precast Gel (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... His6-tagged proteins were then captured from the lysate by Ni2+-IDA chromatography (Profinity IMAC resin, Bio-Rad) under gravity flow and washed with 35mM Imidazole ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates (30 µg) were run approximately one inch into a 4-15% bis-acrylamide gel (Bio-Rad) and processed for in-gel digestion as previously described (60) ...
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bioRxiv - Neuroscience 2022Quote: ... total protein concentration of spinal cord lysates was measured by the DC Protein Assay (Bio-Rad Cat # 5000111). We aliquoted 1 mg proteins per replicate and incubated them with 250 µL pre-washed streptavidin magnetic beads at 4 °C for 12-h to enrich biotinylated proteins ...
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bioRxiv - Cancer Biology 2022Quote: ... Denatured lysates were loaded into 4-20% Mini-Protean ® TGX™ electrophoresis gels (Bio-Rad, Hercules, CA), and SDS-PAGE was run at 150 V for 1-1.5 h ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal total protein concentration were heated at 90°C in 1 × loading buffer (Bio-Rad 1610747) for ten minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Worm lysates were electrophoresed on a 4-20% pre-cast SDS-polyacrylamide gel (Mini-Protean TGX Gels, Biorad) and electroblotted onto a nitrocellulose membrane (Trans-Blot Turbo Transfer Pack ...
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bioRxiv - Microbiology 2019Quote: ... Whole cell lysates were resolved by SDS-PAGE in 4-20% gradient Mini-PROTEAN TGX gels (Bio-Rad) and transferred to 0.2 μm pore size nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified from the lysate of the bacterial cells by NGC Chromatography system (BioRad, CA, USA) with nickel column ...
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bioRxiv - Synthetic Biology 2021Quote: ... After 30 min centrifugation at 4 °C protein concentration of the lysate was determined by Bradford assay (Biorad). In parallel ...
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bioRxiv - Systems Biology 2019Quote: ... SDS PAGE separated 60µg of PC3 cell lysate per well with Precision Plus Protein Standards ladder (1610374, Biorad). Gel pieces were cut by razor blade ...
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bioRxiv - Biophysics 2021Quote: ... The cleared lysates were first run on precast protein gels (PN:456-1096 or 456-1093, Bio-Rad) in MOPS SDS running buffer (B0001 ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were separated in a 4%–15% SDS-PAGE gel and transferred onto a nitrocellulose membrane (Bio-Rad). Membranes were blocked with 5% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysate protein samples (40 µg) were resolved on Criterion 4–20% gradient denaturing gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Protein lysates were diluted to 10 µg per sample and boiled in 1x Laemli Sample Buffer (Bio-Rad). Samples were loaded on pre-cast 4-20% TGX polyacrylamide gels (Criterion ...
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bioRxiv - Cancer Biology 2021Quote: ... and whole-cell lysates (25 µg) were separated on 10% SDS–PAGE and blotted onto PVDF membranes (BioRad Trans-Blot® Turbo™ transfer pack #1704157) ...
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bioRxiv - Immunology 2021Quote: ... Protein lysates (10 μl/lane) were separated on a 4-20% Mini-PROTEAN TGX gel (Bio-Rad Laboratories) and transferred to a polyvinylidene difluoride membrane (Immobilon-P PVDF ...
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bioRxiv - Cell Biology 2020Quote: Total cell lysates and purified sumoylated proteins were resolved by SDS-PAGE using 4-20% gradient gels (BioRad). Western analyses were performed with mouse anti-FLAG (1:2500 ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysates were separated by SDS PAGE using a 4-20% Tris-glycine polyacrylamide pre-cast gel (BioRad) and transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2022Quote: ... Whole-cell lysates were subjected to SDS–PAGE on 4–20% gradient gels (Mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Whole-cell lysates were subjected to SDS–PAGE on 4–20% gradient gels (Mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2022Quote: ... and 1 µL brain lysate (1ug/uL stock determined by BCA (5000001, Bio-Rad Protein Assay Kit I). After 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μg of cell lysates were loaded on pre-cast gradient gels (4–20 %, Bio-Rad, Hercules, USA). Proteins were transferred to methanol-activated polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Proteins or lysates were resolved on a 4-20% gradient SDS-polyacrylamide gel (Criterion TGX 5671094, Bio-Rad), transferred in Towbin(59 ...
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bioRxiv - Physiology 2022Quote: ... The protein concentration in the cell lysate was determined using protein assay (DC Protein Assay, Biorad, Hercules, CA). Samples were adjusted to the same concentration with buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and the protein concentrations of the lysates were quantitated using a DC Protein Assay II Kit (BioRad, 5000112.). Protein A Dynabeads (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: Equal amounts of protein lysate were resolved on SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad) using the Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: Samples were prepared for SDS-PAGE by diluting 10 µg of protein lysate in Laemmli buffer (Biorad 1610747) with 5% β-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... and cell lysates were resolved on Mini-PROTEAN or criterion TGX Stain-Free™ precast gels (Bio-Rad). For immunoblotting ...
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bioRxiv - Neuroscience 2022Quote: Protein concentrations of the total cell lysates of lumbar DRG were determined using a Bradford assay (Bio-Rad). Protein samples (40 μg ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein lysates were collected 72 hours post-transfection by direct addition of 2x Laemmli sample buffer (BioRad #1610737EDU) followed by lysis at 95°C for 10 mins ...
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bioRxiv - Neuroscience 2022Quote: ... After obtaining the brain and Neuro2A lysates we measure protein concentration (DC™ Protein Assay Kit II, Biorad) and samples were used in subsequent experiments.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lysates and BSA samples were added to Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, 5000006) and incubated for 10 minutes before absorbance was measured at 595 nm on a plate reader ...
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bioRxiv - Microbiology 2023Quote: ... lysates were electroblotted onto Trans-Blot 0.2-μm-pore-size pure nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA) by use of a Bio-Rad Mini Trans-Blot electrophoretic transfer cell ...
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bioRxiv - Immunology 2023Quote: ... Ten micrograms of each lysate were separated on Mini-PROTEAN TGS precast protein gels 4-15% (Bio-Rad) and electrotransferred to nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: Equal amounts of lysates (20-35 μg) were loaded onto a precast 4-12% gradient gel (Bio-Rad) and submitted to electrophoresis in NuPAGE buffer (150 V for 1.5 h) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were mixed with the reducing sample buffer for electrophoresis and subsequently transferred onto nitrocellulose membranes (Bio-Rad). Equal loading was checked using Ponceau Red solution ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate protein concentrations were determined using the RC-DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). Equal quantities of protein were separated on SDS-PAGE gels and transferred to the PVDF membranes (Merck) ...
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were cleared by centrifugation and protein concentrations were determined using the DC Protein Assay (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Protein lysates (20 μg/lane) were separated on a 4-20% Mini-PROTEAN TGX gel (Bio-Rad Laboratories) and transferred to a polyvinylidene difluoride membrane (Immobilon-P PVDF ...
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bioRxiv - Microbiology 2023Quote: ... Lysate was thereafter boiled at 100°C for 10 minutes and resolved by SDS-PAGE (Bio-Rad #5671094) and transferred onto a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: Western Blotting: Equal amounts of lysates were loaded onto Mini-Protean TGX (4-20%) SDS-PAGE gels (BioRad). All blots except phospho-MTFR1L were transferred to nitroceullose membranes (BioRad ...