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Citations for Bio-Rad :
801 - 850 of 942 citations for 9 Z 12 E OCTADECADIENOIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated to 95°C for 5 min and then analyzed on a 4-12% gel (Bio-Rad Laboratories, Hercules, CA) using SDS running buffer ...
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bioRxiv - Cancer Biology 2021Quote: Fifteen µg of protein per sample was separated on a BioRad Mini-protein 4-15% precast 12 well 20 µl gels (4561085, Bio-Rad, CA) and electrophoretically transferred to Odyssey Nitrocellulose membrane (P/N 926-31092 ...
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bioRxiv - Cancer Biology 2020Quote: ... 50μg of proteins were electrophoresed in 8% or 12% polyacrylamide gel and then transferred to PVDF membranes (Bio-Rad Laboratory, CA, USA) overnight ...
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bioRxiv - Cancer Biology 2022Quote: Whole cell extracts of NB4 and HT93 were prepared using urea lysis buffer and 30-60μg total protein was loaded on a denaturing 12%-polyacrylamide gel self-cast gel (Bio-Rad Laboratories, CA). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Equal volumes of sample were separated on 4–12% or 10% Criterion XT Bis-Tris Precast gels (Bio-Rad, Hercules, CA, USA) using XT MES Running buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Microbiology 2022Quote: ... Laemmli buffer was added to samples.10 μL of samples were loaded onto 12 % Mini-P ROTEAN® TGX™ Precast Protein Gels (Biorad). Silver staining was carried out at room temperature for 1 h and gels were developed for 2 to 3 mins ...
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bioRxiv - Neuroscience 2023Quote: ... were loaded onto 4%–12% sodium dodecyl sulfate–polyacrylamide gels (Absin, China) for 40 min using an electrophoresis apparatus (Bio-Rad, USA) and transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Laemmli buffer was added to all fractions before boiling the samples and loading them onto a 4-12% Bis-Tris Criterion™ acrylamide gel (Bio-Rad) and run in MOPS buffer (50 mM MOPS ...
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bioRxiv - Microbiology 2023Quote: ... A normalized volume equivalent to 20 μg total protein in the predigested sample was resolved on a 4-12% Criterion XT Bis-Tris gel (Bio-Rad 3450124) at 100V for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained in different cycles overnight at 4°C with the following antibodies: anti-CD3 (clone CD3-12; #MCA1477, Bio-Rad, USA), anti-CD206 (clone E6T5J ...
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bioRxiv - Biochemistry 2023Quote: ... and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD, Cat# 5678084) and transferred to nitrocellulose membranes which were subsequently blocked by 5% BSA for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of protein from each lysate were resolved on 10% or 12% sodium dodecyl sulphate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: Volumes of lysates corresponding to 20 μg of protein were then run on a Criterion XT 4- 12% Bis-Tris Precast Gel (Bio-Rad, 3450124) in MES running buffer (50 mM MES ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then separated on 10-12% SDS-PAGE gels and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans Blot Turbo™ transfer system (Bio-Rad). Membranes were blocked and primary antibodies were diluted in 2% BSA in Tris-buffered saline (TBS) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled at 100 °C for 5 min and loaded in handcrafted 8% SDS-polyacrylamide gel or 4-12% Criterion™ XT Bis-Tris XT Precast Gels (Bio-Rad) and electrophoresed in 1X Tris/Glycine/SDS buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were denatured at 95°C for 10 mins and subjected to SDS-PAGE in a 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad) using XT MES Running Buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Migration of 20 μg of lysate supernatants was performed in 4-12 % gradient or in 10 % SDS-PAGE gels (Bio-Rad Laboratories), transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µg of extracted proteins mixed with 2× SDS sample buffer were separated on 10% SDS PAGE gel (Mini PROTEAN TGX Precast Gel, 10%, 12-well comb; BioRad, 456-1035) and transferred to a nitrocellulose membrane (Amersham Protran Premium ECL 0.45 µm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total protein amount of 100 μg for each sample was separated on 12% Criterion gels (Bio-Rad Laboratories, Hercules, Ca, USA), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled at 95°C for 5 min and loaded into 4-12% Criterion XT-Bis-Tris polyacrylamide gels (Bio-Rad 3450125). Gel electrophoresis was performed in 1X MES running buffer (Bio-Rad 1610789 ...
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bioRxiv - Genetics 2023Quote: ... Putative genomic deletions in the Fo-w and Fo-cn knockout lines were evaluated by amplifying 12 ng of gDNA in 50 μl PCR reactions using a C1000 Touch Thermal Cycler (BioRad, Berkeley, CA). All reactions were carried out to the manufacturer’s recommendations using Ex Taq polymerase (Takara Bio ...
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bioRxiv - Cell Biology 2024Quote: 40 μg of protein lysate was resolved on NuPAGE Novex 4-12 % Bis-Tris gels and transferred onto nitrocellulose membranes (Bio-Rad system). Membranes were blocked with 5 % BSA in TBS-T (10 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Lysates were separated by NuPAGE 4- 12 % Bis-Tris Gels (Thermo Fischer Scientific) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer Systems (Bio-Rad Laboratories). Membranes were blocked with Odyssey Blocking Buffer (Li-Cor ...
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was stopped with 1N sulfuric acid and absorbance was measured at 450 nm in the iMarkTM Microplate Absorbance Reader (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... and destained with 30% methanol,10% glacial acetic acid in double distilled water and subjected to film by ChemiDoc systerm (BioRad).
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bioRxiv - Microbiology 2019Quote: Aliquots of extracted nucleic acid from plasma were processed in triplicate (1.5ul per replicate) following the recommended workflow (WX200 ddPCR system, Bio-Rad). Custom PrimeTime (IDT ...
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bioRxiv - Physiology 2020Quote: ... Samples and standards were incubated with bicinchonic acid for 30 minutes prior to measuring absorbance as 540 nm using an iMark microplate absorbance reader (BioRad).
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bioRxiv - Microbiology 2022Quote: ... A Bio-Rad Aminex HPX-87H HPLC column for organic acid analysis (300 × 7.8 mm; Bio-Rad Hercules, CA, USA) was equilibrated with 5 mM H2SO4 (Titrisol ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was then destained for 15 minutes in 7.5% (v/v) acetic acid before being imaged on a Chemidoc imaging system (Bio-Rad). For western blots ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were Coomassie stained and destained with 10% acetic acid prior to imaging on a ChemiDoc MP imaging system (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was measured using a bicinchoninic acid (BCA) protein assay (Pierce) or Bradford assay (Bio-Rad, Cat. No. 5000006). Proteins were prepared in 1× Laemmli buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed on a 4-15% polyacrylamide NuPage Bis-Tris gel (invitrogen) in 1x morpholineethanesulfonic acid (MES) SDS running buffer (invitrogen) or on a 4-20% polyacrylamide Criterion gels (BioRad) in 1x Tris/glycine/SDS buffer (BioRad ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted proteins were separated by NuPAGE 4-12% Bis-Tris gel in MES SDS running buffer and transferred to a PVDF membrane (Bio-Rad, 162-0263). Tubulin beta chain protein was detected with an anti-TUBB antibody (1:500 in 5% fat-free milk solution in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories, Hercules, CA). Separated proteins were transferred onto nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2019Quote: Twenty-five micrograms of each renal cortex fraction was added to 10% or 12% polyacrylamide gels (Mini-protean TGX precast gels, Bio-rad, CA, USA), and assessed using infrared secondary probes (LICOR ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg samples were boiled in gel loading buffer containing β-mercaptoethanol as a reducing agent and loaded onto a 12% or “AnyKD” precast polyacrylamide gel (Bio-Rad 4569033) for western blot analysis ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled at 100°C for 5 min prior to SDS-PAGE separation on 12% acrylamide Mini-protean TGX precast gels (Bio-Rad Laboratories, US) and Western blot analysis.
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bioRxiv - Neuroscience 2022Quote: ... The nascent and total protein samples were then run on a 4-12% gradient SDS-polyacrylamide gel along with Precision Plus Protein Kaleidoscope ladder (Bio-Rad, Cat #1610395). Then the proteins were transferred onto nitrocellulose membranes using either a Bio-Rad Trans-Blot Turbo Transfer system (25V ...
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bioRxiv - Bioengineering 2022Quote: ... and an equivalent amount of protein lysate (12-15 µg) was electrophoresed on a 4-12% NuPAGE gradient gel and transferred onto low florescent polyvinylidene difluoride membranes (Bio-Rad, Cat: 1704274). Immunoblotting was performed with primary antibodies followed by secondary antibodies with the indicated dilutions ...
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bioRxiv - Microbiology 2022Quote: The protein samples denatured in 1x SDS sample buffer were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) in a 12% gel before blotted to polyvinylidene difluoride (PVDF) membrane by the wet transblot system (Bio-Rad, California, USA). The blocked membrane was incubated with the primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein (5–20 μg) was resolved onto 8–12% SDS-PAGE and blotted onto 0.2 μm pore size PVDF membranes (1620177, Bio-Rad, Hercules, CA, USA). Primary antibodies were incubated overnight at 4°C after blocking membranes for 1h ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were boiled (95°C) and 18 µL were loaded onto a 4-20% Mini-PROTEAN®TGX™ 12 well-gel (Biorad®). After transfer onto a PVDF (Amersham Hybond ...
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bioRxiv - Cell Biology 2020Quote: ... Equal proportions of supernatants and pellets were run on 4-20 % gradient or 12 % SDS-polyacrylamide gels (Mini-PROTEAN TGX Precast Gels, Bio-Rad Laboratories Inc.), which were then stained with Coomassie Blue ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled and loaded onto a 12% precast Tris-Tricine gel in a mini-Protean apparatus (both from Bio-Rad, Hercules, CA). Gels were stained using SimplyBlue SafeStain (Thermo Fisher) ...