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Citations for Bio-Rad :
601 - 650 of 942 citations for 9 Z 12 E OCTADECADIENOIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... containing 2μL of 20X reducing buffer at 95°C for 10 min and separated by SDS-PAGE short migration on 12% SDS-PAGE (Criterion XT Precast gels–BioRad). After a short migration ...
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bioRxiv - Biochemistry 2020Quote: ... Samples (1 µg total protein) were loaded onto non-reducing 12% Mini-PROTEAN® TGX™ precast gels (Bio-Rad), and molecular weight shifts and relative band intensity used to detect the presence and relative abundance of the crosslinked complex ...
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bioRxiv - Biochemistry 2021Quote: ... Protein samples were separated on 10 or 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... PAA gels with a Young’s modulus of 12 kPa were prepared by mixing 18.8% of 40% w/v acrylamide (Bio-Rad, #1610140), 8% of 2% w/v bis-acrylamide (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction mixture was incubated for 30 minutes at 30°C and stopped by adding 12 μl of Laemmli sample buffer (Biorad). After HMT reactions ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cell lysates and 5 μl of purified viruses were diluted in Laemmli buffer and loaded on a pre-casted Criterion XT 4-12% gradient gel (Biorad). Gels were transfered to PVDF membrane using a wet-electroblotting chamber system (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins in loading buffer were boiled for 5 min before loading on to 12-15% SDS-PAGE gels using the Mini-Protean III gel system (BioRad). A protein transfer apparatus (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were resolved using 4-12% BisTris-PAGE and transferred to a PVDF membranes (Bio-Rad catalogue number 162-0177) using a Gel Transfer Cell (BioRad catalogue number 1703930) ...
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bioRxiv - Cell Biology 2021Quote: Samples were run on 8-12% SDS-PAGE gel and transferred to a nitrocellulose membrane using a Trans-Blot Turbo Transfer System (BioRad). Membrane was blocked in 5% milk in TBS + 0.1% Tween blocking solution ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in RIPA buffer and protein extracts from equal numbers of cells were resolved on a 12% SDS-PAGE gel (Biorad), followed by transfer onto Immun-Blot PVDF membranes (Biorad) ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were separated by SDS-PAGE (10-12% acrylamide) and transferred onto a PVDF membrane using the semidry TransBlot system (BioRad). The membrane was blocked with either 5% skimmed milk or 5% BSA (Bovine Serum Albumin ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 20 μg of total proteins in tissue lysates were separated on electrophoresis using 4 - 12% gradient SDS-polyacrylamide gels (BioRad) and transferred to the PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... equal amounts of protein (2.5 or 10 µg) were separated by SDS-PAGE on 4-12% Bis-Tris or 3-8% Tris acetate gels (Criterion XT, BioRad) and transferred to nitrocellulose using TransBlot Turbo (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR was achieved in 12 cycles and at optimized melting temperature (tm) of 68.2 °C thorough C1000 touch thermal cycler (Bio-rad). For single stranded PCR product (ssPCRP ...
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bioRxiv - Molecular Biology 2022Quote: ... and equal amounts of proteins were subjected to SDS-PAGE (TGX Stin Free FastCast Acrylamid, 12%, Bio-Rad, 161-0185) and transferred onto nitrocellulose membrane (Transblot Turbo ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates in Laemmli buffer were separated by electrophoresis on 12% SDS-PAGE gels and transferred for 2 hours at 4°C on to a PVDF membrane (BioRad). After blocking for an hour in 5% dry milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were separated by SDS-PAGE on a 12%-acrylamide gel and transferred to Amersham Protran 0.2 µM nitrocellulose membranes using a Mini Trans-Blot cell (Bio-Rad) for 1 h at 100 V at 4°C in chilled transfer buffer (Tris-Base 3 g/L ...
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bioRxiv - Microbiology 2023Quote: ... Samples were ran on either 10% (for GFP-COMMD2) or 12% (for fraction quality controls) polyacrylamide SDS PAGE gels (BioRad), transferred to PVDF membrane (BioRad TransBlot) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10-30 μg of proteins were loaded in Bolt 4-12 % Bis-Tris gels (Thermo) followed by transferring onto a 0.2 mm PVDF membrane (Bio-Rad) at 25 V ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were loaded on a 12 % SDS-PAGE gel in the Mini-PROTEAN Tetra Cell (Bio-Rad, 165-8000). Protein was then transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Sample preparation of CVA9 VPs for in-gel digestion prior to mass spectrometry analyses and the assessment of the VPs degradation following Subtilisin A treatment on native CVA9 virions have been done using 12% Mini-Protean TGX precast gel (4568043, Biorad). Prior to SDS-PAGE analysis of VPs degradation products by Subtilisin A ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were deposited onto 26-well 12% Bis-Tris Criteroin XT precasted gels (Bio-Rad Laboratories Inc., Hercules, CA, USA) and electrotransferred onto a nitrocellulose membrane with a semidry electrotransfer system (Schleicher & Schuell BioScience ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein per sample was resolved on a NuPage 4-12% Bis-Tris polyacrylamide mini gel (Bio-Rad) and transferred to a nitrocellulose membrane in Towbin transfer buffer containing 20% MeOH at 100V for 1 hour or 25V for 16 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were run on 12 % SDS-PAGE gels and transferred to 0.45 µm pore nitrocellulose membranes (Bio-Rad 1620251) using a wet transfer system (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were separated via 12% SDS-PAGE under reducing conditions and transferred to a polyvinylidene difluoride (PVDF) membrane using “Wed blotting system” (BioRad). After incubation with primary and secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: Samples were resolved by SDSPAGE using either 10 or 12% acrylamide/bis-acrylamide gels and transferred to 0.22 µm nitrocellulose (Bio-Rad). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
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bioRxiv - Plant Biology 2023Quote: ... either on 7–12% Tris-glycine gels or gradient NuPAGE 4–12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4–15%) (BioRad). A list of antibodies and their working dilution used in this work are reported in (Table S3) ...
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bioRxiv - Biochemistry 2023Quote: Protein samples from differential centrifugation and sucrose gradient fractionation as well as cell lysates from APE1 knockdown experiments were resolved on 10 or 12% polyacrylamide gel and then transferred onto AmershamTM ProtranTM nitrocellulose membrane using a wet transfer system (BIORAD) for 1 h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... All samples collected were heated to 95°C for 5 minutes before resolving on a 12% mini-PROTEAN precast gel (BioRad). A Western blot using polyclonal EssR antiserum was conducted following the protocol outlined in the Western blot method section below ...
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bioRxiv - Biochemistry 2023Quote: ... Protein in the supernatant was determined using Bradford assay and 30 µg loaded into 4-10% or 4-12% gradient SDS-PAGE gels (Mini-PROTEAN TGX Precast Gels, BioRad). Proteins were then transferred to 0.45 µm PVDF membranes (Immobilon-P ...
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bioRxiv - Microbiology 2023Quote: ... were separated on a 4 to 12% gradient XT precast Criterion gel using XT-MOPS buffer (1.0 mm thick 4 to 12% polyacrylamide Criterion Bis-Tris XT-gels, Bio-Rad) at 150–200V ...
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bioRxiv - Molecular Biology 2024Quote: ... 95 °C) and 1.25 µg sample was resolved on a 4-12% acrylamide Criterion XT Bis-Tris gel (Bio-Rad) in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured for 10 minutes at 70°C and proteins were separated (55min, 200V) on a Midi 4-12% Bis-Tris gel (BioRad) in 1x MOPS buffer (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... eluate was incubated at 70 °C then run on 4-12% NuPAGE gell and transferred to LF PVDF membrane (BioRad). Western-blot analysis were done with the next antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Extracts were then electrophoresed on 12% Tris-acrylamide SDS-PAGE gel and blotted onto a PVDF membrane (#1620177, Bio-Rad). Membranes were blocked with 5% milk solution for 1 hour at room temperature and probed with primary antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by electrophoresis through 12% agarose polyacrylamide gels and then transferred to PVDF membranes (Bio-Rad Cat #1620175) by electroblotting ...
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bioRxiv - Biochemistry 2019Quote: ... the EtBr-prestained gel was first processed for nucleic-acid content using the Gel Doc system (Bio-Rad) equipped with a 302 nm excitation source and a 520-640 nm band-pass emission filter before Instant Blue protein staining.
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were eluted with 6 mM H2SO4 at a flow rate of 0.6 ml/min from an Aminex HPX 87H organic acid analysis column (300 by 7.8 mm) (BioRad). We recorded the areas for all noticeable peaks associated with detected metabolites (glucose ...
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bioRxiv - Synthetic Biology 2022Quote: ... MD) equipped with a refractive index detector and a HPX-87H organic acid column (Bio-Rad, Hercules, CA) with the following operating conditions ...
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bioRxiv - Biochemistry 2022Quote: ... in 1% (v/v) acetic acid] for 1 min and photographed using Gel Doc XR system (Bio-Rad).
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bioRxiv - Developmental Biology 2023Quote: ... Isolated RNA was used to synthesize complementary deoxyribonucleic acid (cDNA) with the iScripttm cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... folic acid 500 μg/L) or on Columbia agar solid support enriched with 5% horse blood (COH) (Biorad). Anoxic conditions were generated in Gaspack (BD ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... Supernatant was collected and the protein sample concentration was calculated using a bicinchoninic acid (BCA) assay (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... For immunohistochemistry (IHC) experiments following primary antibodies were used: IgG anti-human CD3 antibody (1:200, clone CD3-12, Bio-Rad) and anti-PCNA antibody (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... 12 µl of IP sample and 2.5 µl of input (also boiled in 2X Laemmli Sample buffer with DTT) were separated via SDS-PAGE on 12% pre-cast gels (Bio-rad).
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bioRxiv - Developmental Biology 2020Quote: ... and 50 µg of denatured proteins were run on a 12% acrylamide gel and transferred to a 0.2 µm nitrocellulose membrane (BioRad, Hercules, CA). We blocked membranes in 5% Milk in TBST (Tris Buffer Solution + 0.1% Tween ...
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bioRxiv - Biochemistry 2022Quote: ... 25% v/v glycerol and 0.25% v/v bromophenol blue) was added to each sample prior to resolution on 12 % Mini-PROTEAN TGX Gels (Bio-Rad) by polyacrylamide gel electrophoresis (PAGE ...