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Citations for Bio-Rad :
8401 - 8450 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... RNA was reverse transcribed into cDNA via iScriptTM Reverse Transcription Supermix and real-time quantitative PCR was performed with SsoAdvancedTM Universal SYBR® Green Supermix in a CFX96 Touch™ thermocycler (all kits and devices from Bio-Rad Laboratories) using 10 ng of cDNA as input ...
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bioRxiv - Biochemistry 2019Quote: ... The microsomal fraction was aliquoted and stored at −80 °C after protein quantification with the Quick Start Bradford Protein Assay Kit (Bio-Rad Laboratories, Munich, Germany).
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bioRxiv - Molecular Biology 2019Quote: ... 500 ng of RNA was then used to generate first strand complimentary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). The cDNA was diluted 1:10 before usage and amplified on a LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with iScript cDNA Synthesis Kit and amplified by real-time quantitative PCR with IQ-SYBR Green supermix (Bio-Rad, Hercules, CA, USA). ΔΔCt method was used for relative mRNA quantization by applying the equation 2-ΔΔCt.
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bioRxiv - Genetics 2020Quote: ... and 200-500 nanograms of total RNA was programmed for cDNA synthesis using an iScript™ cDNA Synthesis Kit (#1708890 Bio-Rad; Hercules, CA). Universal SYBR Green Supermix (Bio-Rad)-based qRT-PCR was carried out in a CFX386 Touch™Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... at the same concentration and a volume corresponding to 500 ng was added to iScript™ cDNA synthesis kit reagents (Bio-Rad cat# 1708890) for cDNA synthesis ...
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bioRxiv - Biophysics 2021Quote: ... Real-time quantitative PCR was performed with SsoAdvancedTM Universal SYBR® Green Supermix in a CFX96 Touch™ thermocycler (all kits and devices from Bio-Rad Laboratories) using 10 ng of cDNA/ reaction ...
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bioRxiv - Genetics 2021Quote: ... followed by purification with RNeasy minicolumns (Qiagen, μg total RNA was reverse-transcribed using an iScript cDNA synthesis kit (Bio-Rad, Des Plaines, IL). Assay of RNA via quantitative PCR (qPCR ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories, Hercules, CA) and the Taqman primers Cftr (Mm00445197_m1) ...
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bioRxiv - Immunology 2020Quote: ... and gene expression was examined with a Bio-Rad iCycler Optical System using iQ™ SYBR green real-time PCR kit (Bio-Rad Laboratories, Inc.). The data were normalized to 18S ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Chemiluminescence signals were visualized using the enhanced chemiluminescence kit (ECL; #32106; Thermo Fischer) and a Chemidoc XRS+ scanner system (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated from 200 ng of total RNA purified from high and low heme midguts (using a Bio-Rad iScript cDNA synthesis kit) by priming the reaction with random hexamers to ensure the capture of Sgm derived transcripts ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed using a SYBR Green PCR Master Mix kit on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). The reaction system (10 μL ...
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bioRxiv - Neuroscience 2023Quote: ... frozen in liquid nitrogen and stored at −80°C until the RNA isolation with Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA, USA), as described (Makarava et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... cell viability was determined using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) by measuring the absorbance at 450 nm using an iMarkTM microplate absorbance reader (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Physiology 2024Quote: ... The aqueous layer containing RNA was then mixed with isopropanol and loaded to Aurum Total RNA Mini Kit columns (Bio-Rad, Mississauga, ON, Canada). Total RNA was then extracted according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was subjected to RT-qPCR analysis using the ChamQ Universal SYBR qPCR master mix kit (Vazyme) in a CFX96™ Real-Time System (BioRad, Hercules, CA, USA). RNA from three biological replicates were analyzed and four technical replicates were performed ...
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bioRxiv - Neuroscience 2024Quote: ... Up to 1000 ng of RNA was used to synthesise cDNA using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA, #1725035) following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were physically disrupted in liquid nitrogen before adding lysis buffer from the Aurum Total RNA Mini Kit (catalog #732-6820, Bio-Rad, Hercules, CA, U.S.A.). Further homogenization was conducted using a TissueRuptor II (catalog #9002755 ...
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bioRxiv - Immunology 2024Quote: ... as detailed in “RNA/DNA extraction and sequencing.” Kars RNA expression was measured in each tumor using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad Cat. No. 1725150) and the Quantstudio 6 Flex System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, 1725121). Each reaction proceeded at 50 °C for 2 min ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein concentration of pooled saliva samples was measured to be approximately 20 mg/mL using the Bradford assay kit (Bio-Rad, Hercules, CA, USA) and bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 uL of the extracted genomic DNA were used for qPCR and HRM analysis using the IQ SYBR® Green Supermix kit (BioRad, Cat no. 1708882). Mutation positive male sperm were used to fertilize wildtype eggs which were saved in culture beakers and raised until next spawn ...
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bioRxiv - Physiology 2023Quote: ... The aqueous layer containing RNA was then mixed with isopropanol and loaded to Aurum Total RNA Mini Kit columns (Bio-Rad, Mississauga, ON, Canada). Total RNA was then extracted according to the manufacturer’s instructions and concentration was assessed on the nanodrop attachment for the Varioskan LUX Multimode Microplate reader (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of total RNA was reverse-transcribed to single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad Hercules, CA, USA). RT-qPCR was run on Lightcycler 480 (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... Total protein concentrations of the cell extracts were determined using the Bio-Rad Protein Assay Kit according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA) using BSA as standard.
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bioRxiv - Plant Biology 2023Quote: ... The qPCR was performed using the DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo-scientific) with CFX96 Touch Real-Time PCR Detection System (Bio-Rad Hercules, CA, USA) according to the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of high-quality RNA was used for the first-strand cDNA synthesis by using an iScript™ cDNA synthesis kit (Bio-Rad Laboratories, USA). Primer specificity was ensured by performing a BLASTN search of each sequence against the Solanum lycopersicum ITAGv2.40 database (Supplementary Table S1) ...
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bioRxiv - Immunology 2023Quote: ... The viral genome of the H7N1 virus was quantified using qRT-PCR that was conducted with the Bio-Rad iTaq™ Universal SYBR ® Green One-Step Kit (BioRad, California, USA) using primers designed for the detection of the M gene as published elsewhere 60.
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bioRxiv - Genetics 2023Quote: ... RNA samples were normalised to the same concentration of 250 ng/μL and used to synthesise the complementary DNA using an iScript cDNA synthesis kit (Bio-Rad, catalogue no.: 1708891) following the manufacturer’s instructions ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... Mini kit (Macherery-Magel, Germany) and used to prepare complementary DNA (cDNA) using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, Hercules, CA, USA) on ABS Proflex Thermal cycler (Thermo-fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of RNA were then used for cDNA synthesis with the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, catalog number 1725038). RT-qPCR analysis was performed on the CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using 100 ng of purified total RNA in a 20 μL volume reaction with an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA), as described in (52) ...
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bioRxiv - Physiology 2024Quote: ... Purified RNA was then used for cDNA synthesis via reverse transcription as per protocol by iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). All PCR reactions (25 µL ...
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bioRxiv - Genetics 2024Quote: ... The membranes were then incubated with ECL (Easysee Western Blot Kit, China) and visualized with an Imaging System (Bio-Rad, Universal Hood II, USA).
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bioRxiv - Microbiology 2024Quote: Proteins separated by SDS-PAGE were transferred to a PVDF membrane using the Trans-Blot® Turbo RTA Mini PVDF Transfer Kit (Bio-Rad Laboratories, Inc.). Membranes after activating with methanol were blocked with bovine serum albumin (Amresco ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was prepared from 1 μg total RNA using iScriptTM Reverse Transcription Supermix (Bio-Rad, cat#1708841), then diluted 10-fold and used directly as the template for quantitative real-time RT-PCR (qRT-PCR) ...
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bioRxiv - Microbiology 2020Quote: ... Detection via secondary antibody was done with goat α-rabbit mAb (1:5000) conjugated to HRP (Biorad). Immunoblots were developed using the SuperSignal West Femto (Thermo ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM pH 8.0 by two cycles of centrifugal gel filtration (Biospin mini columns, 6,000 MWCO, Biorad). The nsp’s were buffer-exchanged into 250-500 mM NH4OAc ...
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bioRxiv - Cell Biology 2020Quote: ... StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500, Bio-Rad, Cat# 12005870, RRID: AB_2884949) and hFAB Rhodamine Anti-Tubulin Antibody (1:2500 ...
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bioRxiv - Microbiology 2020Quote: ... and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). % recovery was calculated as 2 raised to the adjusted input Ct minus IP Ct multiplied by 100 ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used for western blot and IF include: goat anti-rabbit HRP (1:10,000, BioRad), goat anti-mouse HRP (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... as well as Plet1 (1:200; Nordic MUbio MUB1512P conjugated to Bio-Rad ReadiLink 633/655 1351005). Single-cell suspensions for ESCs and EPSCs undergoing ES-to-TSC reprogramming as well as control cell lines (ESC/EPSC day 0 samples ...
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bioRxiv - Developmental Biology 2021Quote: ... A total of 1 µg of RNA was reverse transcribed using iScript reverse transcription supermix (Bio-Rad). Real-time PCR was performed as triplicates on 2 ng of cDNA using SYBR Green master mix (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE and observed by immunoblot using rabbit anti-Ube2N (Bio-Rad, AHP974, 1:1000).
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bioRxiv - Molecular Biology 2019Quote: ... and run in 1×TAE buffer for 50h in CHEF-DRIII System with chiller unit (Bio-Rad) with following parameters ...
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were assessed using 6% polyacrylamide gel (made with 29:1 Acrylamide/Bis Solution, Bio-Rad) electrophoresis ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were then pulsed with 1.8 kV in a 1-mm-gap cuvette (Bio-Rad, USA).
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bioRxiv - Genetics 2019Quote: ... incubated in goat anti-rabbit IgG horseradish peroxidase secondary antibody (1:3000; Bio-Rad, Hercules, CA, USA) for 1 hour at room temperature ...