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Citations for Bio-Rad :
8351 - 8400 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and mixed 1:1 with 2x Laemmli Buffer (Bio-Rad Laboratories, 161-0737). 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were mixed 1:1 with 2X Laemmli sample buffer (Bio-Rad) with 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and rabbit anti-alpha actinin-1 at 1:1000 (Bio-Rad Laboratories #VPA00889). Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration of the cytosolic fraction from each sample was measured using the Bradford assay (Protein Assay Dye Reagent Concentrate; Bio-Rad). After normalization ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Cancer Biology 2021Quote: ... The same amounts of protein extracts were subjected to polyacrylamide gel electrophoresis using the 4% to 20% Mini-Protean TGX gel system (Bio-Rad), transferred to PVDF (0.45 μm pore size ...
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bioRxiv - Evolutionary Biology 2020Quote: Samples were heated at 95°C for 10 minutes before loading into 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and running at 150V for approximately 90 minutes at 4°C in 1X tris-gylcine running buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of protein were loaded and separated by SDS-PAGE gel electrophoresis on 4%-20% Criterion gels (BioRad, Hercules, CA), and then transferred to PVDF membranes ...
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bioRxiv - Genetics 2021Quote: ... 150 μg of protein was then separated on Criterion Precast Gradient 4-15% SDS-PAGE gels (Bio-Rad, cat. number 5671083) and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... Aliquots of larval homogenate were removed prior heat-treatment to measure soluble protein using a Bradford assay (Bio-Rad 500-0006) with BSA standard curves.
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts (30 μg) from each protein sample were separated by SDS-PAGE and transferred to PVDF membranes (PVDF; Biorad, USA). The membranes were blocked with 10% (W/V ...
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bioRxiv - Molecular Biology 2021Quote: ... Ten to twenty micrograms of denatured protein was loaded onto a 4-20% TGX stain-free polyacrylamide gel (Bio-Rad, USA) or NuPAGE 3-8% Tris acetate gel and subsequently transferred onto pre-cut nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins on the gels were transferred onto PVDF membrane using BioRad Mini Trans-Blot system (BioRad, Mini Trans-Blot® Cell). The membranes were washed with pure water and blocked with 5% skimmed milk in 0.1% Tween-20/PBS (PBST ...
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bioRxiv - Evolutionary Biology 2021Quote: ... SDS PAGE was performed using 7.5% or 4-20% Mini-PROTEAN® TGX™ precast protein gels (BIO-RAD, 4561023/4561094) run in running buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... from Sigma was used to quantify triglyceride levels and protein levels were measured using the Quick Start™ Bradford 1X Dye Reagent (Cat. # 500-0205) from Biorad. This was followed by colorimetric estimation using TECAN Infinite M200 pro-multimode plate reader in 96-well format ...
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bioRxiv - Developmental Biology 2020Quote: ... and 50 µg of denatured proteins were run on a 12% acrylamide gel and transferred to a 0.2 µm nitrocellulose membrane (BioRad, Hercules, CA). We blocked membranes in 5% Milk in TBST (Tris Buffer Solution + 0.1% Tween ...
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bioRxiv - Cancer Biology 2022Quote: ... 15-25 μg of proteins were loaded onto 4-12% Mini-PROTEAN Tris-Tricine Precast Gels or Criterion Tris-Tricine Precast Gels (Bio-Rad), and electro-blotted on nitrocellulose membranes (GE Healthcare Life Science) ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (20 μg) were separated using an Any kD gel (SDS-PAGE) and transferred to a nitrocellulose membrane (BioRad, Temse, Belgium). Aspecific binding sites were blocked for 30 min using TBS + 1% casein (BioRad ...
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bioRxiv - Neuroscience 2021Quote: 10-40 µg of denatured protein extracts were separated by SDS-PAGE using 10% acrylamide gels or 4-15% Mini-Protean precast gels (Bio-Rad) for 2 h at 120 V and transferred onto methanol-activated PVDF membranes at 350 mA for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP was normalized to total protein bands per lane (visualized by a stain-free scan on a Bio-rad gel doc). Each experiment was repeated at least two times from biological replicates.
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bioRxiv - Molecular Biology 2020Quote: ... For western blotting, separated proteins were transferred onto a nitrocellulose membrane (Whatman, Dassel, Germany) using a Trans-Blot Turbo transfer system (Bio-Rad). The membrane was blocked and then incubated for 1 h with an His-tag-specific mouse mAb antibody (Proteintech ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were electrophoresed for 70 min at 80 V in a BioRad MINI-PROTEAN Tetra system (BioRad Laboratories, CA, USA), and the gel was stained with Coomassie Brilliant Blue R-250 (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of protein (typically 10 μg) were separated under reducing conditions on 10 or 12 % TGX-polyacrylamide gels (Bio-Rad) and transferred to Hybond-P membrane (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... Extracts were then incubated on ice for 10 min before protein concentration was calculated by Bradford assay (Bio-Rad, 500-0006). Extracts were then mixed with Laemmli buffer and boiled at 95°C for 5 minutes before loading on SDS-PAGE gels.
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bioRxiv - Molecular Biology 2020Quote: ... IB analysis was performed after 6-7.5% SDS-PAGE or 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad, Hercules, CA), with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2021Quote: ... and the equivalent of 50 μg protein input (elution) were analyzed on a 4%-12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins associated with half of the Dynabeads were eluted with 22 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) by boiling at 95 °C for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... were normalized by the level of total proteins in each lysate (determined using the TGX-stain free imaging technology; Bio-rad).
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bioRxiv - Immunology 2021Quote: Recombinant human GFAP protein were run on 12% SDS-PAGE and transferred onto membrane via Trans-Blot Turbo Transfer System (BIO-RAD). Membrane was incubated in blocking buffer (PBS ...
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bioRxiv - Neuroscience 2019Quote: ... The denatured samples were resolved on a 4-20% Mini-PROTEAN TGX Stain-Free precast protein gel (Bio-Rad, cat# 4568094) and transferred to a nitrocellulose membrane (0.45 μm ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed four times with binding buffer containing 0.1% Tween-20 and bound proteins were eluted with 2X Laemmli Sample Buffer (Bio-Rad Laboratories). Eluted proteins were subjected to electrophoresis on a 17% resolving gel at 120V for 2 hrs and transferred to PVDF membrane at 180mA for 2 hrs and immunoblotted with His-Probe-HRP (Pierce ...
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bioRxiv - Genetics 2019Quote: ... membranes were washed three times for five minutes each in TBST and protein detected using Clarity western ECL substrate (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2019Quote: ... protein samples (20 µg of total protein) were separated at 300 V for 30 min using 4-20% gels (Criterion TGX, Bio-Rad), followed by wet transfer to PVDF membranes (0.2 µm Immun-Blot ...
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bioRxiv - Neuroscience 2019Quote: ... Equal amounts of protein per sample (5 or 10ug) was loaded into a 4-20% gradient precast mini-PROTEAN TGX gel (Bio-Rad) and proteins were separated with 200V in 1x running buffer (24.76 mM Tris base ...
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bioRxiv - Physiology 2020Quote: ... equal amounts (30 μg) of protein were resolved on 4–20% TGX stain-free gels (Bio-Rad Laboratories GmbH, Munich, Germany) and transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2019Quote: ... 5-15 µg of protein was resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked in 5% milk in phosphate buffered saline with 0.1% Tween (PBS-T ...
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bioRxiv - Immunology 2019Quote: ... The lysates were transferred to a new Eppendorf tube and total protein in each sample was determined using Lowry assay (Bio-Rad). Equal protein (20ug per lane ...
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bioRxiv - Bioengineering 2019Quote: Equal volumes of (GT)6-Cy5-SWCNT and FAM-tagged protein at 2X working concentration were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% CriterionTM TGXTM precast Midi protein gel (Bio-Rad) for SDS-PAGE followed by wet transfer to nitrocellulose membrane ...
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bioRxiv - Physiology 2020Quote: ... Bradford protein assay kit as well as anti-mouse and anti-rabbit secondary antibodies were obtained from Bio-Rad (Hercules, CA). Antibodies against ERα (F-10 mouse – for WB) ...
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bioRxiv - Cancer Biology 2019Quote: ... Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093, Bio-Rad) and transferred to PVDF membrane (1620177 ...
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bioRxiv - Bioengineering 2020Quote: ... Equal volumes of 10 mg/L (GT)15- or (GT)6-Cy5-SWCNTs and 160 mg/L protein were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Biochemistry 2020Quote: ... was confirmed by both SDS-PAGE using a precast Mini-PROTEIN® TGX Stain-Free™ Gel (Bio-Rad Laboratories, Inc.) and by measuring the direct absorbance at 280 nm using a NanoDrop™ Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... The amount of bound protein was determined by boiling in SDS sample buffer and analysing using Bradford reagent (BioRad, Hercules, CA). The eluted protein was resolved on a BioRad 15% TGX gel ...
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bioRxiv - Immunology 2021Quote: ... Lysates were collected and equal protein amounts were separated by SDS-PAGE and blotted onto a nitrocellulose membrane by wet-blotting (Bio-Rad). Membranes were blocked with TBS-T containing 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... containing the crude synaptosomes was resuspended and processed for western blotting in NP-40 lysis buffer as above with the following modifications: equal amounts of protein per sample (unboiled) were separated on 3-8% Tris-Acetate XT gel (Bio-Rad) in XT Tricine running buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... of total protein for each sample were separated by SDS-PAGE (10% acrylamide gel) and transferred to a PVDF membrane (Bio-Rad). The membrane was blocked in TBST buffer containing 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were subjected to SDS-PAGE and the proteins in the gel were transferred to a PBDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). After probing the membrane with the primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated using SDS-polyacrylamide gel electrophoresis (SDS-PAGE-4-12%) and transblotted onto nitrocellulose membranes (Bio-rad, California, USA). The membranes were blocked with 5% dry milk (overnight ...