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Citations for Bio-Rad :
8151 - 8200 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and the nanocluster size was derived by eluting 20.0 μL of the sample with PBS buffer at a flow rate of 0.5 mL/min and calibrating the chromatograph with that of the protein standards (Bio-Rad). The hydrodynamic size and zeta potential of supraclusters were determined using a Zetasizer NANO ZS-90 instrument (Malvern) ...
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bioRxiv - Biochemistry 2022Quote: ... proteins in gels were transferred to a PVDF membrane using a Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) and blocked with 5% milk ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted samples and whole cell lysate controls were loaded and run on a 10% Mini-PROTEAN TGX protein gel (Biorad), visualized with AcquaStain (Bulldog Bio) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged at 14,000g for 15 min at 4°C and supernatant protein concentrations were determined by Bradford assay (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... proteins were transferred to nitrocellulose membrane from SDS- or native-PAGE gels using the Transblot Turbo semidry transfer apparatus (BioRad) at 25V for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were boiled with SDS-PAGE sample loading buffer (6X SDS) and 30 ug of protein was loaded to each well of a 10% Tris-glycine SDS-PAGE gel (BioRad). Proteins were transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: Quantification of protein levels from western blot bands was done using the Evolution-Capt Edge software (Vilber) or ImageLab software (Biorad). The data presented are expressed as means ± SD ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Cell Biology 2022Quote: ... 30 and 36 hpi respectively on a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (4561083, Bio-Rad). Blotting was performed using the Trans-Blot Turbo Mini 0.2 μm Nitrocellulose Transfer Pack (1704158 ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad). Membranes were blocked in 10% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
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bioRxiv - Genetics 2022Quote: ... the antibodies were prepared by mixing 3 ug of antibody HNRNPK and IgG (as a negative control in a separate tube) with 650 ul of Dilution Buffer and 50 ul of Surebeads Protein G magnetic Beads (BioRad) and left in rotation for 30 minutes at 4 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... Ribosome fractions were resolved by SDS-polyacrylamide gel electrophoresis on 10% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad). Prior to loading ...
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bioRxiv - Physiology 2022Quote: ... the electrophoresed proteins were transferred from SDS-agarose gel to a nitrocellulose membrane (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with a semi-dry western blot system (Pierce Power Blotter ...
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bioRxiv - Neuroscience 2022Quote: 10 μL of tissue samples (25 μg of protein per well) were placed into each well of the Western blot gel (Biorad Criterion TGX Stain free 4-15% 18 wells ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts of total protein were loaded on a 4-20% gradient SDS-PAGE gel (Mini Protean TGX, Bio-Rad), then blotted onto nitrocellulose membranes and probed with anti-villin mouse monoclonal antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 20 μg of total proteins in tissue lysates were separated on electrophoresis using 4 - 12% gradient SDS-polyacrylamide gels (BioRad) and transferred to the PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were subsequently separated by electrophoresis on precast gels (4-20% mini protein TGX gels, Bio-Rad catalog #456-1094) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted protein concentration was estimated using a Bradford assay (Quick Start™ Bradford 1x Dye Reagent #5000205; Bio-Rad).
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bioRxiv - Plant Biology 2022Quote: ... approximately 60-70 μg of protein was loaded onto 7 cm pH 3-10 NL and pH 4-7 IPG strips (BioRad) and subjected to first-dimensional electrophoresis with increasing voltages from 50V to 2000V ...
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bioRxiv - Plant Biology 2023Quote: ... An equal amount of protein bands were manually appointed per lane and adjusted in the Lane profile menu of the Image Lab software (BioRad). Band intensity was quantified relative to the unreacted control sample.
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bioRxiv - Neuroscience 2022Quote: ... Absorbance at 450 nm was measured in a plate reader (Thermo) and the total protein content of the samples was obtained by the Bradford assay (Biorad). As GDNF is not expressed in the mouse cortex ...
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bioRxiv - Microbiology 2023Quote: ... the Stain-Free visualization was enabled by activation of the gels for 45 seconds with UV-light before the proteins were transferred by Trans-Blot Turbo Transfer (Biorad) onto a nitrocellulose membrane (Trans-Blot Turbo RTA ...
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bioRxiv - Plant Biology 2023Quote: Protein concentration was measured via Bradford assay using a Quick Start™ Bradford 1x Dye Reagent (Biorad, Cat. No. 5000205) prior to protein content normalization ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted protein samples were checked by SDS-PAGE and Coomassie brilliant blue staining (Bio-Safe™ Coomassie Stain, Bio-Rad). The protein concentration was determined with the Bradford method (Quick Start™ Bradford Reagent ...
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bioRxiv - Microbiology 2024Quote: Protein samples were incubated at 95°C for 10 minutes before being resolved on TGX Stain-free gels (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to a nitrocellulose membrane (Cytiva) using the semi-dry Trans-Blot Turbo Transfer system (Bio-Rad). The membrane was blocked in 5% milk in TBS-T (tris-buffered saline ...
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bioRxiv - Immunology 2023Quote: ... Buffer containing eluted protein was filtered through a 0.22-μm spin filter and directly loaded on the Enrich SEC650 column (Bio-Rad) for SEC ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a PVDF membrane using the Bio-Rad Turbo Transblot System and transfer kit (Bio-Rad Laboratories) and fixed in methanol prior to blocking in 5% non-fat milk in TNT buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... were resolved by SDS-PAGE using stain-free precast gels (4-15% Mini-Protean Tgx Stain-Free Protein Gels, Biorad). Gels were then activated by Ultraviolet light with the ChemiDocTM Touch Imaging System (Biorad ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and equal amounts of proteins were subjected to SDS-PAGE (TGX Stin Free FastCast Acrylamid, 12%, Bio-Rad, 161-0185) and transferred onto nitrocellulose membrane (Transblot Turbo ...
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bioRxiv - Cell Biology 2022Quote: ... Total cell lysates were prepared with icecold lysis buffer and the protein concentration was measured by Coomassie binding (Bio-Rad). Equal amounts of protein were added to each well of a G-LISA plate and incubated for 30 min at 4°C ...
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bioRxiv - Physiology 2022Quote: Equal amounts of protein extracts were resolved by SDS–PAGE using 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to PVDF membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... The luminescence was calculated as an average of three technical replicates and normalized to the total protein levels quantified using the Bradford’s method (Bio-Rad). Results are from the two or three biological replicas and are expressed as a percentage of the normalized luminescence of the control.
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bioRxiv - Cancer Biology 2022Quote: ... Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad) staining.
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... A representative size-exclusion chromatography chromatogram is shown in Figure S4 with a gel filtration protein standard (Bio-Rad Laboratories). The fractions from the single monomer peak were combined and diluted ten-fold and buffer exchanged three times into 10 mM sodium phosphate buffer at pH 6.5 with an Amicon Ultra-15 Centrifugal Filter Unit Ultracel-10 (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Cell equivalents of ~1.5×106 cells per lane were separated by 10% SDS-PAGE along with the Precision Plus Protein Kaleidoscope standard (BioRad). The fractionated proteins were transferred to a PVDF membrane ...
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bioRxiv - Cancer Biology 2023Quote: All binding assays were performed on a ProteOn XPR36 SPR Protein Interaction Array System (Bio-Rad Laboratories, Hercules, CA, USA). The instrument temperature was set at 25 °C for all kinetic analyses ...
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bioRxiv - Neuroscience 2024Quote: ... Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad) using a turbo transfer system (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... The proteins were incubated for one hour at 65 and 70°C in a thermocycler with heated lid (T100, BioRad) Binding to D25 and MPE8 antibodies was measured afterwards following the protocol described above.
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bioRxiv - Physiology 2023Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated on 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼40 min followed by 110 V for ∼70 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and then proteins were transferred to a nitrocellulose membrane using a wet Trans-Blot system (Bio-Rad, Hercules, CA, USA). The primary antibodies used were anti-slow MyHC (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... 20μg of total protein per fraction were collected and diluted with their respective solvents and 4X Laemmli buffer (Biorad, 1610747) to obtain aliquots of equal volume ...
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to a hybond ECL nitrocellulose membrane using a wet transfer system (Bio-Rad Laboratories, Hercules, CA, USA). The membrane was incubated in α-Ty antibody (1:10 ...
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bioRxiv - Molecular Biology 2024Quote: ... and purity was determined using SDS-PAGE on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) stained with Biosafe Coomassie (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... proteins separated in SDS-PAGE gels were transferred onto a PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked in 5% (w/v ...