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Citations for Bio-Rad :
7951 - 7983 of 7983 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Protein/Histone extracts were denatured by heating at 95°C for 5 minutes in 1x Laemmli buffer (Bio-Rad, Feldkirchen, Germany, cat#1610747) and subsequently separated via 15% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Molecular Biology 2024Quote: Protein samples (5 µg) were separated by SDS-PAGE at 150V for 50 minutes (12%, TGX™ FastCast™ Acrylamide Kit; Bio-Rad). Primary antibodies targeting Gpx4 (1:1000 ...
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bioRxiv - Genetics 2024Quote: ... which were dried on tissue paper before homogenizing whole individuals in 100 µL of 5% Chelex-100 resin (Bio-Rad, Hercules, CA, USA) in ultrapure water with 2.5 µL proteinase K (20 mg/mL ...
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bioRxiv - Microbiology 2021Quote: The 20 μl RT-dPCR reaction mixture consisted of 5 μl One-Step RT-dPCR Advanced Supermix for Probes (Bio-Rad, Laboratories, Munich, Germany), 2 µl of Reverse Transcriptase (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... were incubated together at RT for 5 min in the presence and absence of 5 mM 2-OG prior to loading on the analytical ENrich 650 column (BioRad Laboratories, Inc., Hecules, USA). The column was equilibrated with 50 mM Tris/HCl and 150 mM NaCl and supplemented with 5 mM 2-oxoglutarate when the complexes were preincubated in the presence of 5 mM 2-oxoglutarate and the applied proteins isocratic eluted with the respective buffer with a flow rate of 1 ml min-1 ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed in triplicate in a total volume of 20 μL that included 2 μL of the template or standard plasmid and 250 nM of each primer and TaqMan (5’ hydrolysis) probe (the RT-qPCR reactions also included 0.5 μL of Bio-Rad iScript advanced reverse transcriptase). Thermocycling conditions for qPCR consisted of an initial 50 °C step for 2 min followed by 95 °C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 300nM final volume of each forward and reverse primer pair, 5 μL SYBR Green Supermix (50% total volume of reaction, rxn) (Bio-Rad Inc. Hercules, CA), and nuclease-free water (to make a final reaction volume of 10 μL) ...
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bioRxiv - Cell Biology 2021Quote: The supernatants of cell patches were collected after culturing under normoxic or hypoxic (5% O2) conditions and analysed using a fluorescence-dyed microsphere-based immune assay (Bio-Rad Laboratories, Hercules, CA) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The re-suspended DNA droplet solution was then denatured at 95 °C for at least 5 min on a C1000 Touch™ Thermal Cycler from Bio-Rad (CA, USA). The aqueous solution consisted of 25% v/v SUV solution (containing 8mM lipids as prepared above ...
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bioRxiv - Microbiology 2020Quote: ... as per the manufacturer’s running conditions then activated for 5 min under UV to determine total protein (Bio-Rad ChemiDoc XR, Image Lab Software). Proteins were transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... washed in TBST and incubated with a recombinant human monoclonal anti-PAR antibody (1:2,500 in 5% nonfat dry milk-TBST; clones #19 and #21, highly specific for target-bound PAR, custom-designed at Bio-Rad AbD Serotec GmbH) (39 ...
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bioRxiv - Cell Biology 2022Quote: ... the mixes were boiled at 97°C for 5 min and loaded in a Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hércules, EE.UU) at 12% acrylamide/bis-acrylamide ...
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bioRxiv - Physiology 2022Quote: ... such that a total of 2 μg protein in 20 μL of buffer was loaded into wells of a Mini-PROTEAN TGX Stain-Free gel (5-14%) (Bio-Rad, Mississauga, ON, Canada). Protein ladder (5 μL of Precision Plus Protein™ All Blue Prestained Protein Standards ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression for the 5 marker genes was quantified by qPCR using SYBR Green PCR Master Mix (Bio-Rad Laboratories Inc., Hercules, CA, USA), in three independent samples per treatment ...
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bioRxiv - Cell Biology 2023Quote: ... They were then washed thrice with TBST (5 minutes/wash) at room temperature and signal developed for one minute using Clarity™ Western ECL substrate (Bio-Rad, Cat #: 1705060) or Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then washed thrice with TBST (5 minutes/wash) at room temperature and then incubated with HRP-conjugated goat anti-rabbit IgG (H + L) (Bio-Rad, Cat #: 170-6515) or goat anti-mouse IgG (H + L ...
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bioRxiv - Cell Biology 2023Quote: ... samples were boiled at 97°C for 5 min and run on a 12% Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hercules, USA) for 1 hour at 150V ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...
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bioRxiv - Microbiology 2023Quote: ... followed by 40 cycles (95°C for 5 seconds, 60°C for 40 seconds) on a CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA). The housekeeping gene GAPDH (PY17X_1330200 ...
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bioRxiv - Immunology 2024Quote: ... The 1:10 diluted plasmid was combined with C41 cells (∼3 x 109 competent cells) and incubated on ice for 5 min followed by electroporation using a Bio-Rad Electroporator (Bio-Rad, Hercules, CA, USA). The transformation was incubated at 37°C in LB media for 1 hr prior to spreading onto LB agar plates containing kanamycin ...
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bioRxiv - Plant Biology 2024Quote: ... Samples equivalent to 1 µg chlorophyll were solubilized with 2X sample buffer (Laemmli buffer + 6M urea + β-mercaptoethanol + Bromophenol blue) for 5 min at 75°C and loaded onto 12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). After protein separation ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted at a concentration of 5 ng/µl and 2 µl were loaded in duplicate in a 96-well qPCR plate with the reaction mix comprising 5 µl of Sybr Green master mix (Bio-Rad, Hercules, CA, USA), 2 µl of water and 1 µl of primer mix containing forward and reverse primers for STAT3 and P53 ...
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bioRxiv - Cell Biology 2021Quote: ... A 5-μL aliquot of the pre-amplified reaction was analyzed by qPCR using SsoFast EvaGreen Supermix (Bio-Rad Life Science, Inc., Hercules, CA, USA) and an Applied Biosystems 7900HT qPCR instrument (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... exposed to enhanced chemiluminescence plus west pico for 5 min and autoradiographed in a BioRad Gel-Doc System (Bio-Rad Laboratories Inc., Hercules, CA, USA). The loading consistency was verified utilizing the house-keeping proteins (Rab11 and β-Actin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were dissolved in 25 μL of double distilled water (boiled at 95 °C for 5 minutes) containing loading buffer (4X; Bio-Rad Laboratories, Mississauga, ON, Canada) and reducing agent (20X ...
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bioRxiv - Microbiology 2024Quote: ... Electroporation was carried out by mixing 45 µl of competent cells with 100-200 ng of plasmid and subjecting the cells to a short 5 ms electric pulse under a 12500 V/cm electric field in a Gene Pulser II® (Bio-Rad, Hercules, CA, USA) (2500 v ...
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bioRxiv - Plant Biology 2024Quote: ... Tungsten beads were coated with 5 µg of plasmid shot on the plates using the PDS-100/He system (Bio-Rad,Hercules, CA, USA; 1672257) with 1550 psi rupture disks ...
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bioRxiv - Neuroscience 2024Quote: ... First strand cDNA was diluted 1:1000 and 5 μL was used as template in droplet digital PCR (ddPCR) using ddPCR Supermix for Probes (no dUTP; Bio-Rad, Des Plaines, IL, USA) and TaqMan Gene Expression Assay probes (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: GV3101 electro-competent Agrobacterium tumefaciens strain was incubated on ice with ~100 ng plasmid for 5 min and electroporated using a MicroPulser (Bio-Rad Laboratories; 2.2 kV, 5.9 ms). Immediately after electroporation ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed using iQ™ SYBR®Green Supermix on an iQ™5 multicolor real-time PCR detection system (Bio-Rad, CA, USA). All primers were obtained from Sigma-Aldrich and described in Suppl ...
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bioRxiv - Plant Biology 2024Quote: ... Six μL cDNA (diluted 1:5 with distilled water) was used per qRT-PCR reaction in a 15-μL reaction volume using SYBR Green (BIO-RAD, iTaq Universal SYBR Green Supermix) and a BIO-RAD CFX96 real time PCR instrument ...
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bioRxiv - Biochemistry 2021Quote: ... All final samples were boiled in 30 µL NuPAGE LDS sample buffer and sample reducing agent for 5 min and loaded on Mini-PROTEAN® TGX Stain-Free™ Gels (Bio-Rad laboratories, Hercules, CA, USA). Electrophoresis was performed at 300 V for 3 min using the BIO-RAD Mini-PROTEAN® Tetra System ...