Labshake search
Citations for Bio-Rad :
7701 - 7750 of 7983 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were washed 3 times for 5 min with TBST after secondary antibody incubation and chemiluminescence signal was detected using Clarity Western ECL Substrate (Bio-Rad). All relevant information on antibodies used in this study are listed in Table S2.
-
bioRxiv - Bioengineering 2021Quote: ... This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad) for 1 h with rocking ...
-
bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...
-
Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
-
bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 min and proteins were resolved on 10% SDS polyacrylamide gels and blotted onto nitrocellulose membranes (Bio-Rad). Anti-rabbit Phospho-p44/42 MAPK antibody (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2021Quote: ... Assays comprising of premixtures of a forward and reverse primer (18 μM each) with a FAM- or HEX-conjugated hydrolysis probe (5 μM) were either purchased from Bio-Rad, based on previous publications (Roberts et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... Gene expression analysis were performed by quantitative RT-qPCR in a Viia7PCR device with 5 μl reaction mix containing SyberGreen Supermix (Bio-Rad). Tomato actin was used as a reference gene and fold change in expression were calculated based on 2^(-ddCt ...
-
bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
-
bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
-
bioRxiv - Immunology 2021Quote: ... The total PCR reaction volume was 15 µL per well (10 µL master mix + 5 µL cDNA sample) and the qPCR was performed on the CFX384 Touch Real-Time PCR Detection System (Bio-Rad) using the cycling conditions listed below:
-
bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed again for 3 × 5-min in TBS-T before being immersed for 5 min under gentle agitation at room temperature in Clarity ECL detection substrate (Bio-rad Laboratories Pty Ltd ...
-
bioRxiv - Cell Biology 2022Quote: ... Standard qPCR reactions with 5 or 10 ng cDNA per reaction were performed with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX Connect Real-Time PCR Detection System ...
-
bioRxiv - Molecular Biology 2022Quote: ... Gels were run at 90 V for 120 min and there-after stained with Ethidium bromide at room temperature for 5 min and visualised using a Gel Doc XR imaging system (Bio-Rad).
-
bioRxiv - Molecular Biology 2022Quote: ... for 30 minutes at room temperature was followed by three 5 minute washes in milliQ water before imaging on a ChemiDoc MP (Bio-Rad).
-
bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude) ...
-
bioRxiv - Cell Biology 2022Quote: ... denatured in 2x Laemmli buffer (5 min at +95°C) and 20 µg were loaded per well of Mini-PROTEAN Precast Gel (Bio-Rad). The protein transfer was done with the Trans-Blot Turbo system (Bio-Rad) ...
-
bioRxiv - Cell Biology 2022Quote: ... The stoichiometry of condensin II subunits for each preparation was checked by 5% SDS-PAGE followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining ...
-
bioRxiv - Cell Biology 2022Quote: ... supplemented with beta-mercaptoethanol and denaturated at 95 °C for 5 min before loading on a 12 % PAA precast gel (#4561044, Bio-Rad). The gel was run at 70 V for 15 min and then 100 V for 1 h ...
-
bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with appropriate primary and secondary antibodies listed in Table 2 in 5% milk and developed using the Clarity Western enhanced chemiluminescence substrate system (1705060, Bio-Rad) in a Xograph Compact X5 processor ...
-
bioRxiv - Microbiology 2020Quote: Total protein extracts (5 × 106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) [55] ...
-
bioRxiv - Developmental Biology 2020Quote: ... Total protein lysates were prepared from 25 pair of testes and loaded in equal dilution series (5, 10, and 20 μg) onto precast stain-free PAAG gel (Bio-Rad). After electrophoresis ...
-
bioRxiv - Biochemistry 2021Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
-
bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25% of beads by heating at 95°C for 5 minutes in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from the remaining 75% of beads using TRIzol (Thermo Fisher) ...
-
bioRxiv - Microbiology 2020Quote: ... The lysates were boiled for 5 min at 95°C in 1x XT sample buffer containing 1x XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT precast gels (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: Protein samples were denatured for 5 min at 95 °C and run on Mini or Midi TGX-Stain-free precast gels (Bio-rad) at 110V for 60 min (mini gels ...
-
bioRxiv - Immunology 2020Quote: ... polβ and Exo1 to the 5’Sμ (in CH12-F3) was assessed by quantifying ChIP-enriched DNA using CFX384 realtime PCR (Bio-Rad). The following primers were used for qPCR:
-
bioRxiv - Microbiology 2021Quote: ... Membranes were washed four times in PBST for 5 minutes at room temperature and imaged directly using a ChemiDoc MP imaging system (Bio-Rad).
-
bioRxiv - Microbiology 2022Quote: ... 14 µL of PCR product were then transformed by electroporation in 90 µL cells (2.5 kV electric pulse for 5 ms, Bio-Rad micropulser). Cells were recovered in SOC outgrowth medium (NEB B9020S ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips pH 5-8 for CDK4 (BioRad, Hercules, CA, USA) or pH 3–10 for CDK2 (Amersham Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... Transfection took place over two rounds of electroporation at 850 V and 25 μF with an interval of 5 secs (Bio-Rad Gene Pulser Xcell Electroporation Systems) ...
-
bioRxiv - Microbiology 2020Quote: ... The membranes were blocked 1 hr at room temperature with Odyssey PBS Blocking Buffer (Li-Cor, 927-40000) or in 5% Blotting-Grade Blocker (Biorad, 1706404) in TBST (50 mM Tris buffer ...
-
bioRxiv - Synthetic Biology 2020Quote: ... under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA). Alternatively ...
-
bioRxiv - Molecular Biology 2020Quote: ... The blots were washed three times with TBS-T for 5 min each time before to development with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging on the ChemiDoc XRS+ System (Bio-Rad).
-
Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... The incubation was terminated by boiling the samples at 95°C for 5 minutes in 1X Laemmli sample buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol (Bio-Rad) ...
-
bioRxiv - Plant Biology 2021Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (rat (IgM)/LM20 and LM19 mouse (IgG)/2F4 (in 5% milk PBS) and after washing chemiluminescence induced with Clarity Western ECL Substrate (Biorad, #1705061) and detected with ChemiDoc imaging system (Biorad).
-
bioRxiv - Synthetic Biology 2020Quote: ... The samples were run with 5 mM H2SO4 at 0.6 mL/min on an Aminex HPX-87H (Biorad Inc., CA, USA) column at 50°C ...
-
bioRxiv - Microbiology 2020Quote: ... Membranes were washed four times in PBST for 5 minutes at room temperature with agitation and imaged directly using a ChemiDoc MP imaging system (Bio-Rad). Alternatively ...
-
bioRxiv - Neuroscience 2020Quote: ... Membranes were subsequently blocked with 1x TBS containing 0.1% Tween 20 (1x TBST) with 5% (w/v) blotting-grade blocker (Bio-Rad, 1706404) and probed with the primary antibody of interest diluted in 1x TBS containing 0.1% Tween 20 and 0.5% blocker at room temperature overnight ...
-
bioRxiv - Immunology 2021Quote: ... P2 was dialyzed against 1% glycine containing 2% ampholytes pH 3-10 (first run) or pH 5-7 (second run) and then loaded onto a liquid isoelectric focusing system (Rotofor, Bio-Rad). Individual fractions were harvested and their pH and absorbance at 280nm were measured ...
-
bioRxiv - Microbiology 2021Quote: ... About 0.5 μg of plasmid DNA were mixed with the cell suspension and transferred to ice-cold 1mm gap cuvettes (Bio-Rad). Cells were electroporated in a Bio-Rad Gene Pulser Xcell system using settings ...
-
bioRxiv - Cancer Biology 2020Quote: ... The beads were washed five times with IP lysis buffer and were boiled for 5-10 min in 2 times Laemmli sample buffer (#1610737, Bio-Rad) with 2-mercaptoethanol (#BP176-100 ...
-
bioRxiv - Bioengineering 2020Quote: ... followed by 40 cycles of 95°C for 5 s and 60°C for 30 s (Bio-Rad CFX manager 3.1). Quantification of mRNA levels of different genes was performed using the 2−ΔΔct method ...
-
bioRxiv - Neuroscience 2021Quote: ... membranes were washed in PBS-T for three times 5 min and developed through chemiluminescence using a ChemiDoc XRS (Bio-Rad).
-
bioRxiv - Developmental Biology 2021Quote: ... or 400 ng of total RNA was reverse transcribed to cDNA in a 20 µL reverse transcription reaction using 5 µL of iScript reverse transcription supermix (1708841, Bio-Rad Laboratories - Life Sciences ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were washed four times in PBST for 5 minutes at room temperature and imaged directly using a ChemiDoc MP imaging system (Bio-Rad).
-
bioRxiv - Neuroscience 2020Quote: ... Blots were washed 3x for 5 min each in TBS without Tween and incubated with goat anti-mouse IgG coupled to horseradish peroxidase (BioRad, #170656) at a 1:3000 dilution in blocking buffer for 1 h ...
-
bioRxiv - Neuroscience 2021Quote: ... Blots were incubated with Pierce Super Signal or Millipore Immobilon enhanced chemiluminescence reagents for 5 min and visualised using a ChemiDoc system (Bio-Rad).
-
bioRxiv - Genetics 2021Quote: ... Samples were denatured at 100°C for 10 min and 5% of samples were loaded and run on a 10% Mini-Protean TGX gel (Bio-Rad). Electrophoresed proteins were transferred onto a nitrocellulose membrane using Turbo-blot Transfer system (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... The gel was stained with 5 μg/ml ethidium bromide for 20 min and imaged using the ChemiDoc XRS+ system (Bio-Rad).