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Citations for Bio-Rad :
7801 - 7850 of 9474 citations for Recombinant Human ITGAV & ITGB6 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates and lipid raft protein isolates were prepared by sonication in 4x Laemmli sample buffer (Bio-Rad, 1610747) and then loaded into 10% SDS-polyacrylamide gels for electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... the lysate that contains 10 μg of protein per sample was mixed with 2x Laemmli Sample Buffer (Bio-Rad) supplemented with 2 M 2-Mercaptoethanol (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein levels were quantified to normalize between each well using Bradford reagent (Bio-Rad, Marnes-la-Coquette, France).
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bioRxiv - Systems Biology 2022Quote: ... The supernantant was then added to a new Eppendorf and protein concentration was assessed using the Bradford assay (Biorad).
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were visualized on a 4-15% gradient SDS-PAGE TGX Stain-Free gel (Bio-Rad 456-8086) and then stained with Coomassie blue (Bio-Rad 1610786) ...
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bioRxiv - Biophysics 2022Quote: ... The protein-dye conjugate was purified by 3 rounds of size exclusion chromatography (Bio-Spin 6 column, BioRad, CA) and then aliquoted in 5 μL portions and stored at -80C until required.
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bioRxiv - Molecular Biology 2022Quote: ... under reducing conditions and proteins transferred to nitrocellulose membranes using a semidry Trans-Blot Turbo Transfer System (Bio-Rad). Antibody reactions were carried out in 1% skimmed milk in PBS with 0.1% Tween-20 and washed in PBS with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were then centrifuged at 12,000 rpm at 4°C for 20min and total protein extracts were boiled in Laemmli sample buffer (BioRad), separated by SDS-PAGE under reducing conditions and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were electrophoretically transferred onto a polyvinylidene fluoride (PVDF) membrane using Semidry Trans-Blot apparatus (Bio-Rad, Hercules, CA) at 20 V for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... in equal volume and then subjected to electrophoresis using the 7.5% Mini-Protean TGX Precast Protein Gels (Bio-RAD) in a non-denaturing buffer ...
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bioRxiv - Microbiology 2022Quote: ... the samples were washed extensively with lysis buffer and the proteins were eluted by boiling 5 ul of the resin with 5x SDS loading buffer and the proteins were detected by western blot analysis using rabbit antibody to FLAG (1:2,000, BioRad) or mouse antibody to Myc (1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated using a gradient SDS PAGE gel (8-12%) and a Mini-Protean 3 Cell (Bio-Rad) at 85 V ...
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bioRxiv - Microbiology 2022Quote: ... total protein extracts were subjected to SDS page electrophoresis using 4-20% polyacrylamide gradient gels (Bio-Rad, Watford, UK) and transferred to nitrocellulose membranes using a Trans-blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Biophysics 2022Quote: ... 10 µL of 12x and 120x diluted protein prep was then loaded into a 15% SDS-PAGE gel (BioRad) with BioRad Precision Plus unstained standard for comparison ...
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bioRxiv - Biochemistry 2022Quote: ... 40 μg of total protein was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad 1620177). Membranes were blocked for 1 h in 5% milk in 1X PBST (or 5% BSA in 1X PBST for western blots of immunoprecipitations ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µg of protein was separated by SDS-PAGE using pre-cast TGX 4-15% gradient gels (BioRad). Protein was transferred to a 0.45 µm nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL protein was injected onto either a self-packed buffer exchange column (P6 polyacrylamide gel, Bio-Rad Laboratories) or a prototype desalting column from Thermo Fisher Scientific and online buffer exchanged to 200 mM ammonium acetate ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred from the remainder of the gel to nitrocellulose membrane using the Trans-Blot Turbo (Bio-Rad). Membranes were incubated in a 5% milk in 1X Tris-buffered saline ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins where eluted from the beads by directly adding 20 µL of Laemli Sample Buffer buffer 2X (Biorad, #1610747). Samples where then processed for SDS-PAGE and Western blotting.
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bioRxiv - Biochemistry 2020Quote: ... Protein bands were quantified on a BioRad Chemidoc Touch Imaging System using Image Lab software version 6.0.1 (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... Data were normalized to GAPDH expression or total protein determined via UV treatment of TGX Stain-Free gels (Biorad) followed by detection on the transfer membrane.
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were separated on a SDS-PAGE Mini-PROTEAN TGX 4-15% gradient gel (Bio-Rad, catalog #456-1086) along a 1:1 mix of Amersham ECL Rainbow Molecular Weight Markers (Amersham # 95040-114 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were denatured and proteins separated in SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Fifteen μg of protein per sample were separated in 4-20% SDS-polyacrylamide gel (BIO-RAD mini-protean-TGX) before electrophoretic transfer onto a nitrocellulose blotting membrane (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... the remaining interphase was dried completely before resuspending in Tris-buffered saline (50mM Tris-Cl, 150mM NaCl, pH 7.6) for protein quantification using a Bradford Assay (BioRad). Aqueous phase was evaporated under nitrogen flow and used for LC-MS ...
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Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionbioRxiv - Neuroscience 2021Quote: ... 12.1 ug of protein from each sample was loaded onto a 10% Tris-Glycine gel (Biorad, cat no. 4561034) and then transferred onto a PVDF membrane and blocked for one hour with 5% BSA in 0.1% Tween/1xTBS ...
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bioRxiv - Neuroscience 2021Quote: ... Both the soluble (40 µg of proteins) and the insoluble (4 µl) fractions were spotted on nitrocellulose membrane (BioRad). The near infrared fluorescence signal was acquired by scanning the membrane at the LiCor Odissey using the 800 nm filter and images were analyzed using the Fiji software.
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bioRxiv - Microbiology 2020Quote: ... and the images were analyzed and the signals quantitated after total cell protein normalization using the Image Lab 6.0.1 software (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... and 150 ml Protein A Sepharose (GE, 17-5138-01) in a Poly-Prep Chromatography Column (BioRad, 731-1550). Fabs were generated by Papain cleavage and purified by the use of the Pierce Fab Preparation Kit according to the manufacturers’ instructions (Thermo Scientific - #44985) ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was made with 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels 10-well (BioRad, 4561094) with 30 μg of protein lysate for well and running was 70 V for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... and 30μg of total protein per sample was mixed with Laemmli buffer supplemented with 2-mercaptoethanol (10%) (Bio-rad) and boiled at 99°C for 10 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... A sample containing 1-20µg of protein was loaded into a 4-15% precast gel (Bio-Rad Mini-ProTEAN) and run at 60V for 20min followed by 160V for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were electrophoresed on SDS-PAGE using 4-15% Mini-PROTEAN® TGX™ precast gel (Biorad#456-1085) and transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ Transfer Pack (Biorad#170-4159) ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitated proteins were eluted by heating at 100°C during 15 minutes in 4x Laemmli Sample Buffer (BIORAD). Immunoprecipitated protein complexes were subjected to Western blot analysis ...
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bioRxiv - Immunology 2020Quote: ... Protein (15–100 μg per lane) was separated in polyacrylamide gels containing SDS and transferred to Nitrocellulose membrane (Biorad) and probed with different primary antibodies according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (50 μg) were resolved on 12.5 % SDS-PAGE gels and via electrophoretic transfer system (Bio-Rad, München, Germany) bonds moved to polyvinylidene fluoride (PVDF ...
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bioRxiv - Biophysics 2021Quote: ... coli HU protein was monitored using 20% SDS-PAGE and the concentration was determined using the Lorry assay (Biorad). The purified E ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and transferred onto nitrocellulose membrane (Bio-Rad) using TransBlot Turbo (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... Eight micrograms of total protein lysate were resolved on 4–15% Mini-PROTEAN TGX Stain-free gels (Bio-Rad) and transferred onto Immuno-Blot PVDF membranes (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... The separated protein samples were transferred to the PVDF membranes using a Mini Trans-Blot Electrophoretic Transfer Cell (Biorad) at 100V for 1h ...
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bioRxiv - Pathology 2022Quote: ... The expression levels of target proteins were quantified with Quantity One 1-D Analysis Software (Bio-Rad, Hercules, CA) and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Systems Biology 2022Quote: Samples with ∼30 μg of soluble total proteins were loaded on 4%-20% TGX stain-free precast gels (Biorad). These proteins were then separated by electrophoresis and transferred on PVDF membrane using TurboBlot (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... 30 ug of Protein extracts were separated by 4-12% Bold NuPage (ThermoScientific) and transferred onto PVDF membranes (BioRad). After blocking with 3% albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to 0.2 μm nitrocellulose membranes (BioRad, Cat# 1620112). After blocking the membranes in 2% BSA in Tris buffered saline (TBS)-Tween for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Twenty (HA blot) or forty (SREBP1 blot) micrograms of protein were mixed with Laemmli sample loading buffer (Bio-Rad), heated to 37 °C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-associated fluorescence intensities (arbitrary units, AU) were analyzed using Quantity One image analysis software (BioRad, Hercules, CA, USA). Gels were stained with Colloidal Coomassie Brilliant Blue G250 and scanned.
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 10μg of protein was loaded per sample on a 4-15% SDS-PAGE Stain-Free TGX precast gel (BioRad). Gels were run at 150V for approximately 60 minutes ...