Labshake search
Citations for Bio-Rad :
7751 - 7800 of 9474 citations for Recombinant Human ITGAV & ITGB6 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2019Quote: ... The retention time of both oligomer types was compared with a set of molecular weight protein standards (Bio-Rad) consisting of bovine serum albumin (66.5 kDa) ...
-
bioRxiv - Microbiology 2019Quote: ... Separated proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ transfer system (Bio-Rad). The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche ...
-
bioRxiv - Systems Biology 2020Quote: Whole cell fractions were isolated using RIPA buffer and protein concentrations were determined using a Bradford Assay (Bio-Rad). 20µg whole cell lysate were separated on 8-12% SDS-PAGE gels (depending on the molecular weight of the target proteins) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein for immunoblotting was eluted from ten percent of beads by boiling in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from ninety percent of beads using TRIzol reagent (Thermo Fisher) ...
-
bioRxiv - Plant Biology 2019Quote: ... Total proteins (40 µg) were separated using SDS-PAGE (10 % acrylamide) and then blotted onto nitrocellulose membranes (1620112, Biorad). Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001 ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... A total of 10 µg protein/well was separated by electrophoresis on 12% Mini-PROTEAN TGX gels (Bio-Rad). Proteins were transferred to Immobilon-FL PVDF membranes (Millipore ...
-
bioRxiv - Immunology 2019Quote: ... For blotting the SDS-PAGE separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semi-wet transfer (BioRad) and blocked for 1 h with 2% milk ...
-
bioRxiv - Plant Biology 2019Quote: ... SDS-PAGE and protein transfer to nitrocellulose membranes were performed with a Trans-Blot Turbo Transfer System (BioRad, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... protein samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, 1620115). The membranes were blocked in Tris-buffered saline-Tween 20 containing 5% nonfat milk at room temperature for 1 h ...
-
bioRxiv - Bioengineering 2019Quote: ... and analysed for protein content using 12% SDS-PAGE gels (Mini-PROTEAN TGX Stain-Free Precast Gels, Bio-Rad). Protein gels were imaged using a BioRad Gel Doc EZ Imager and relative protein band intensity was determined using the BioRad ImageLab software.
-
bioRxiv - Genetics 2019Quote: ... Protein samples were run on 15% SDS polyacrylamide gel and transferred to Immun-Blot® PVDF membrane (Bio-Rad) for 1h at 60V ...
-
bioRxiv - Developmental Biology 2019Quote: ... Thirty or 40 µg of protein was resolved on Mini-PROTEAN TGX precast 12% SDS-PAGE gels (Bio-Rad) and proteins were transferred to a nitrocellulose membrane using the Bio-Rad Trans-Blot Turbo Transfer System ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins from peak fractions were pooled and DTT was removed with an Econo-Pac 10DG desalting column (Bio-rad) which was pre-equilibrated with buffer C ...
-
bioRxiv - Biochemistry 2020Quote: Proteins separated by SDS-PAGE were transferred to PVDF membranes using the TRANS-BLOT semidry system from BIO-RAD in transfer buffer (25 mM Tris ...
-
bioRxiv - Cancer Biology 2020Quote: ... 25 μg of protein was then fractionated on Tris–glycine SDS-PAGE gradient (4–15% acrylamide) gels (BioRad; #3450123), transferred onto PVDF membranes (Millipore ...
-
Sex Differences in Brain Tumor Glutamine Metabolism Reveal Sex-Specific Vulnerabilities to TreatmentbioRxiv - Cancer Biology 2021Quote: ... Membranes were washed with PBS-T again and proteins were visualized using a ChemiDoc MP Imaging System (Bio-Rad). Band intensity was quantified using Image Lab Software (Bio-Rad).
-
bioRxiv - Cell Biology 2020Quote: ... Macronutrient measurements were performed in 96-well plates using commercial kits: DC Protein Assay kit (Bio-Rad, 500-0116), Triglyceride Assay kit (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... Equal amounts of protein samples were separated by 9% SDS-PAGE gel and transferred onto nitrocellulose membranes (Bio-Rad). The following primary antibodies were used ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were heated at 55° C for 5 minutes and 30 µg of protein per sample was run on Tris-Tricine 16.5% precast polyacrylamide gels (BioRad) for Aβ or 10% Tris-Glycine (TGX ...
-
bioRxiv - Cell Biology 2021Quote: ... Twenty μg of protein were separated by SDS-PAGE and transferred to 0.2 μm nitrocellulose membranes (BioRad, Cat# 1620112). Membranes were blocked in 2% bovine serum albumin (BSA ...
-
bioRxiv - Molecular Biology 2021Quote: ... Equal protein loading was confirmed by Coomassie staining of polyvinylidene difluoride membranes (Coomassie Brilliant Blue R-250, Bio-Rad).
-
bioRxiv - Biophysics 2020Quote: ... An amount of 12 μg of each protein sample was applied on 4-20% gradient precast gel (BioRad, USA). Gels were stained using Coomassie brilliant blue G-250 (Sigma Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein-containing fractions were concentrated by ultracentrifugation and fractionated by an ENrich SEC650 gel filtration column (Bio-Rad) in binding buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins in column fractions were acetone precipitated and separated by SDS PAGE on 4-15% gradient gels (Bio-Rad) and blotted onto Immobilon PVDF membranes (Merck) ...
-
bioRxiv - Biochemistry 2020Quote: ... The BRPF1 bromodomain protein was purified on glutathione agarose resin in a 25 mL Econo-Column® (Bio-Rad) by first washing the beads extensively with wash buffer containing 20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... individual proteins were buffer exchanged into 200 mM ammonium acetate pH 8.0 either by SEC or using Biospin-6 (BioRad) columns and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
-
bioRxiv - Biochemistry 2020Quote: ... Equal amounts of total protein underwent separation by 10% SDS-PAGE and electro-transfer on nitrocellulose membranes (Bio-Rad). After blocking (5% skim milk in Tris-buffered saline with 0.05% Tween 20 ...
-
bioRxiv - Neuroscience 2021Quote: ... 10-20 µg of total protein was loaded on precast SDS-PAGE gels (4-20% Criterion TGX, Bio-rad) and immobilon-FL membranes (Merck Milipore ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were electrophoresed on SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ precast gel (Biorad #456-1094,), transferred onto methanol pretreated PVDF membrane ...
-
bioRxiv - Cell Biology 2021Quote: ... lysates were cleared by centrifugation at 20,000 x g for 30 min and protein amount was determined by standard Bradford assay (Biorad). For each reaction ...
-
bioRxiv - Cell Biology 2021Quote: ... was added to membranes to visualize proteins by chemiluminescence using X-ray film or a Chemidoc station (Bio-Rad). Each immunoblot was repeated several times using samples from independent experiments ...
-
bioRxiv - Biochemistry 2021Quote: ... Approximately 20 ug of acid-extracted protein was run in triplicate on a 4-20% SDS-PAGE gel (BioRad) and a single band corresponding to P1 and P2 was cut out for processing ...
-
bioRxiv - Physiology 2020Quote: ... and isolated protein complexes were eluted from the beads in 1X SDS sample buffer (Bio-Rad Laboratories, Hercules, CA) at 60°C for 10 min ...
-
Functional characterization of a DNA-dependent AAA ATPase in SimranZ1, a F cluster mycobacteriophagebioRxiv - Biochemistry 2021Quote: ... The protein was then transferred to a PVDF membrane by using Mini-PROTEAN Tetra Cell (Bio-Rad, United States). The membrane was blocked overnight by using 5% BSA at 4 °C followed by incubation with mouse anti-His6 antiserum (Santacruz Biotech ...
-
bioRxiv - Biochemistry 2021Quote: ... and the proteins were purified using a Profinia immobilized metal affinity chromatography (IMAC) column with Ni2+ beads (Bio-Rad) and a Profinia protein purification system (Bio-Rad ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using 12% polyacrylamide gels and transferred onto a PVDF membrane using Trans-Blot Turbo (Bio-Rad). The following primary antibodies were used ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were then electroblotted onto a PVDF membrane using a semidry blotter (trans-blot turbo transfer system; Bio-Rad). Membranes were blocked overnight at 4°C in TBS-T (50 mM Tris-HCl ...
-
bioRxiv - Microbiology 2020Quote: ... 40 µL of supernatant containing 5 µg of proteins were mixed to 36 μL Laemmli Buffer 1x (Bio-Rad) and 4 µL β-mercaptoethanol BioUltra (Sigma ...
-
bioRxiv - Plant Biology 2021Quote: ... and 30 μg of total protein was separated by 12 % SDS-PAGE and blotted onto PVDF membrane (Bio-Rad). The membranes were blocked in 5 % (w/v ...
-
bioRxiv - Systems Biology 2020Quote: ... As a molecular weight marker we utilized the Precision Plus Protein™ Prestained Standard in Dual Color (Biorad, 1610374).
-
bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated samples were separated by SDS-PAGE using Any kD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, 4569034), and then blotted to a PVDF membrane (0.2 μm ...
-
bioRxiv - Neuroscience 2020Quote: ... The protein concentration of each homogenate was measured using a Bradford assay according to the manufacturer’s instructions (Bio-Rad).
-
bioRxiv - Microbiology 2021Quote: ... Proteins were then transferred at room temperature (RT) onto a nitrocellulose membrane using a semi-dry transfer cell (Biorad) (20 V ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein bands were then transferred from the gels onto 0.2 μm polyvinylidene fluoride (PVDF) membranes (Bio-Rad, CA, USA) using semi-dry electroblotting system (Trans-Blot Turbo Transfer System ...
-
bioRxiv - Microbiology 2020Quote: ... 7.5 mg of proteins were diluted in ChIP buffer supplemented with 0.01% SDS and precleared 1 h at 4 °C with 50 μl of protein A agarose beads (BioRad) and 100 μg BSA ...
-
bioRxiv - Microbiology 2020Quote: ... Equal quantities of protein (generally 20-50 μg) were resolved on 4-20% gradient SDS-Polyacrylamide gels (Bio-Rad) and then transferred onto Polyvinylidene difluoride membranes (Millipore Sigma) ...
-
bioRxiv - Microbiology 2020Quote: ... The concentration of the epithelial cell proteins in the resulting supernatant was determined using the Bradford method (Bio-Rad).
-
bioRxiv - Cancer Biology 2020Quote: ... Twenty μg of each protein sample was separated using SDS-gel electrophoresis and transferred onto a 0.45-μm PVDF membrane (BioRad). Membranes were incubated with blocking buffer (5% milk powder dissolved in 1x TBST ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed at 150V for one hour through 7.5% or 4-20% mini-PROTEAN TGX gels (Bio-Rad) and blotted at 300 mA for one hour onto PVDF membranes ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were resolved in SDS-PAGE gel (4-20% gradient polyacrylamide gel electrophoresis, Mini-PROTEAN® Precast Gels, Biorad). Blots were blocked for 1 hour with 5% non-fat dry milk in PBS/0.1% tween-20 (Sigma Aldrich) ...