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Citations for Bio-Rad :
7701 - 7750 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Proteins were blotted onto ready-to-use Trans-Blot Turbo transfer pack using the Trans-Blot Turbo system (BioRad) at 2.5 A (25 V ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins (25 μg/lane) were separated on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories). For the detection of α- syn ...
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bioRxiv - Immunology 2023Quote: The biotinylated ODN-bound proteins were precipitated with streptavidin beads from mitochondria lysates and resolved by PAGE (Bio-rad). The PAGE gels were stained using a silver staining kit (Pierce) ...
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bioRxiv - Genetics 2022Quote: ... Samples were resolved by SDS polyacrylamide gel electrophoresis on 4%-15% CriterionTM TGX Stain-FreeTM Protein Gel (BioRad #5678083) using 30μg of whole cell lysate ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples at a standard protein concentration were diluted in Western blot sample buffer consisting of Laemmli buffer (Bio-Rad) and 5% ý-mercaptoethanol (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (50 µg) were loaded into 4–20% PROTEAN® TGX™ Precast Protein Gels (BioRad, Cat# 5678081). After overnight transfer to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2023Quote: ... 700 µg of each sample were incubated with 30 µL of slurry Affi-Prep protein A resin (Bio-Rad) and 1 µg of mouse anti-V5 tag antibody (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Ten µl of the supernatant were used to determine protein concentrations by the dye-binding Bradford assay (Bio-Rad). Twenty-five µg of total proteins in a final volume of 100 µl were pipetted into a white 96 well plate (Greiner Bio-One ...
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bioRxiv - Biochemistry 2023Quote: Protein lysates were boiled in reducing sample buffer and resolved on 4-20% Criterion TGX polyacrylamide gels (Bio-Rad), then transferred to 0.2 µm nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... then separated from the protein by boiling for 10 mins at 100℃ with 4x Laemmli Sample Buffer (BioRad, #1610747).
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bioRxiv - Cell Biology 2023Quote: ... Migrated proteins were transferred from the gels to PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) and run settings of 2.5A ...
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bioRxiv - Cell Biology 2023Quote: ... 1mg cell lysate or 500 µg cytosolic or nuclear fraction were incubated with 2μg antibody or normal IgG and pulled down by 5µL protein G-magnetic beads (Bio-Rad). Samples were then pulled down by protein-G-magnetic beads and subjected to WB or proteomics analysis ...
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µL samples were separated by 4-15% SDS-PAGE (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad). A mixture of recombinant proteins was used as a standard ...
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bioRxiv - Physiology 2023Quote: ... Total protein (5∼15 µg) was loaded and separated by 4–20% pre-made SDS-PAGE gels (#4568095, BioRad, Mini-PROTEAN TGX Stain-Free Precast Gels ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins (25 μg/lane) were separated on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories). Membranes were immunoblotted for CSF1R (1:250 ...
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bioRxiv - Physiology 2023Quote: ... Equal amounts of protein were fractionated by SDS-PAGE (Mini-PROTEAN TGX Precast 4– 15% gradient gels, Bio-Rad), and eletro-transferred onto nitrocellulose membrane (Trans-Blot Turbo Transfer System ...
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bioRxiv - Neuroscience 2023Quote: ... Protein content of supernatant was quantified via Bradford assay according to the manufacturer’s instructions (catalogue # 500-0006, Bio-Rad). Absorbance was measured at 595nm using a Pherastar FS (BMG LABTECH).
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were quantified by Pierce Reducing Agent Compatible BCA Protein Assay and run on 4-20% TGX gels (Biorad). Membranes were probed with anti-FLAG M2 antibody (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Protein extracts dissolved in Lämmli sample buffer or SDS sample buffer were separated by SDS-PAGE (Biorad Mini Protean) and transferred to ethanol activated Amersham Hybond-P 0.45 µm PVDF membranes (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by 8.5% SDS-PAGE gel electrophoresis and transferred to PVDF membranes (Bio-Rad, Mississauga, ON, Canada). Membranes were blocked with 5% non-fat milk powder in Tris-buffered saline- Tween (TBS-T ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were visualized using fluorescent secondary antibodies and quantified using ChemiDoc MP device (Bio-Rad Laboratories, Hercules, CA, USA) and quantified with Image Lab software (version 6.0 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE on a Mini-PROTEAN TGX Stain-Free pre-cast polyacrylamide gel (Bio-Rad), transferred to a hydrophobic polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: ... 10 µL of each sample was loaded onto a 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run at 180 V for 45 minutes ...
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bioRxiv - Genomics 2023Quote: ... The protein is then transferred to PVDF midi membranes using the Trans-blot Turbo Transfer System (Bio-rad, 1704150).
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bioRxiv - Neuroscience 2023Quote: ... 12% Criterion TGX precast midi protein gels (5671044) and 4x Laemmli sample buffer (1610747) were purchased from Bio-Rad Laboratories (Rishon Le Zion ...
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bioRxiv - Neuroscience 2023Quote: ... Equal protein lysate concentrations (40μg per well) were loaded on a 4%-20% precast Mini-Protean gradient TGX gel (Biorad). Proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-blot turbo transfer system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were electrophoresed under reducing conditions on 4– 15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... 100 ng of each purified protein was mixed with an appropriate volume of 2x Laemmli sample buffer (Bio-Rad) with 5 mM β-mercaptoethanol (Millipore Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins (20 μg per sample) were separated on Mini-PROTEAN TGX 4–15% precast SDS-PAGE gels (Bio-Rad) for 1–3 hours at 100 V and then transferred to PVDF membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... We loaded the lysates at equal protein amounts and concentrations on 4-15% precast TGX gels (Bio-Rad, USA) or 10% hand- poured SDS-PAGE gels ...
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bioRxiv - Developmental Biology 2023Quote: Protein was extracted from the dissected testes using RIPA buffer as described previously and quantified using Bradford reagent (Biorad). Equivalent concentration of lysate was denatured using 1X Laemelli Buffer with 1 M DTT at 95°C ...
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bioRxiv - Immunology 2023Quote: ... and membrane GAS protein fractions were separated on SDS-PAGE (Criterion TGX Gels, 4%–20% precast gels, Bio-Rad) and proteins were transferred to PVDF membranes using the trans-blot turbo transfer system (BioRad ...
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bioRxiv - Developmental Biology 2023Quote: ... and aliquots were 1:50 in water prior to Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were then normalized to 1 mg/mL protein in 0.1% Azo prior to reduction with 30 mM DTT at 37C for 1 h and alkylation with 30 mM chloroacetamide for 45 min ...
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bioRxiv - Plant Biology 2023Quote: ... Samples containing 6 µg total protein were resolved using pre-cast SDS-PAGE Any KD™ gels (BIO-RAD), transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 µm ...
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bioRxiv - Microbiology 2024Quote: ... The protein lysates were centrifuged and the supernatants were subjected to SDS-PAGE (gradient, 4 to 20%; Bio-Rad) and electroblotted onto a nitrocellulose membrane ...
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bioRxiv - Physiology 2024Quote: ... and 30 μg protein were loaded per well in Mini-PROTEAN TGX Stain Free precast gels (Bio-Rad, 4868096). Proteins were transferred to nitrocellulose membranes using Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: hCOs were processed using urea-based lysis buffer and proteins were separated in the SDS-PAGE gels (Bio-Rad) and nitrocellulose membranes.
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bioRxiv - Cell Biology 2024Quote: ... 50-100 µg protein was run on an SDS-PAGE and blotted using a wet-transfer system (Bio-Rad) to a nitrocellulose membrane (0.45 µm ...
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bioRxiv - Genetics 2024Quote: ... Thirty micrograms of denatured cell lysate proteins were loaded for SDS-PAGE electrophoresis (4–20% TGX gel, Bio-Rad). Primary antibodies include ALDH3B2 (Proteintech ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were separated by SDS–PAGE electrophoresis using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) followed by detection of the ubiquitinated substrate by immunoblotting using anti-MBP (New England Biolabs) ...
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bioRxiv - Neuroscience 2024Quote: ... The protein lysates were then loaded onto an 10% Ready Gel® Tris- HCl Precast Gels (BioRad, catalog#1611119) in running Buffer (BioRad ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The same amount of protein was loaded in each lane of Mini-PROTEAN TGX gels (4–20%, Bio-Rad). The antibody signals were detected by chemiluminescence using ImmunoStar LD reagent (290-69904 ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 ug of protein was loaded per lane in a pre-cast gel for SDS-PAGE (Bio-Rad). Gel was then transferred using the TurboBlot Transfer (Bio-Rad ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples prepared with equal amounts of proteins were run on 10% Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033) (for ASGR1 blot ...
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bioRxiv - Microbiology 2024Quote: ... and total protein was wet transferred to a nitrocellulose membrane using a mini Trans-Blot electrophoretic system (Bio-Rad). After the transfer ...
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bioRxiv - Immunology 2024Quote: ... The SEC-eluted protein peaks corresponding to the correct retention volume based on the molecular weight standards (BioRad: 1511901) were collected and concentrated using Amicon Ultra centrifugal filters (Millipore Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were loaded to a 48-well Bio-Dot® microfiltration apparatus (Bio-Rad Laboratories, Hercules, CA, USA) with BioTraceTM NT nitrocellulose membrane (Pall Life Sciences ...