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Citations for Bio-Rad :
7351 - 7400 of 7957 citations for Myeloperoxidase MPO Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Free floating sections were blocked with 3% donkey serum and incubated overnight with rat anti-CD68 (1:200, Bio-Rad). Aβ deposition and CD68 staining were both developed with a Vectastain ABC kit and DAB reaction ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µl of prepared sample containing 6.7 µl synaptosome lysate or 1-1.5 µl SV eluate was subjected to SDS-PAGE on 4-20% gradient gels (Criterion TGX, Bio-Rad) and transferred to a PVDF membrane using a semi-dry blotting apparatus ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were thawed on ice and diluted 1:1000 in 1X Bradford reagent (BioRad, Hercules, CA, Cat. No. 500-0006). Standard curve was determined via serial dilutions of 1 mg/mL BSA diluted in H2O ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was then transferred to a 1 mm cuvette (ice chilled) for electroporation at 1.5 kV for 5 ms using an electroporator (Bio-Rad). Electroporated samples were mixed with 1 ml of SOC medium and transferred into a culture tube containing 1 ml of Brucella broth (BD) ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in 1X Tris-buffered saline (TBS) for 1 hour at room temperature prior to the addition of 0.05% Tween 20 (Bio-Rad) and primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBST and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... 72°C (30s) and 1 cycle of 72°C for 5 min (C1000 Touch Thermal Cycler, Bio-Rad, Hercules, CA). 2–5 μl of the PCR reactions were subjected to gel electrophoresis using a 3% agarose gel in tris-borate-ethylenediamine tetraacetic acid buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were spun down at maximum speed for 1 minute and run on precast 4-20% gradient acrylamide gels (BioRad) in 1X Tris-Glycine buffer containing 0.1% SDS ...
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bioRxiv - Plant Biology 2024Quote: ... prior to bombardment with 10 mg 1 μm tungsten beads treated with 5 μg purified plasmid DNA in a Helios gene gun (BioRad) at 1,550 PSI ...
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bioRxiv - Microbiology 2024Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 hr at 4°C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 0.5 μM of gene-specific primers (Sup Table 1) in a CFX96 qPCR Real-Time PCR module with C1000 Touch Thermal Cycler (BioRad). After 10 minutes at 95°C ...
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bioRxiv - Developmental Biology 2024Quote: ... PVDF membranes were blocked for 1 hour in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4 °C with antibodies listed in Supplementary Table 2 in 5% BSA in TBS-T ...
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bioRxiv - Biophysics 2024Quote: ... The concentration of TP 70 was determined by measuring A230 (1 A230 unit = 240 pmol TP70) by SmartSpec Plus spectrophotometer (BIORAD). Aliquots were flash frozen and stored at −80°C.
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The membrane was rinsed three times with TBS-T and then incubated with goat anti-mouse IgG coupled to horse radish peroxidase (1/10,000, 1706516, Bio-Rad) in TBS-T for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated secondary antibodies used were anti-rabbit and anti-mouse at a dilution of 1:2000 and immunoblots were developed using Chemidoc imaging system (ChemiDoc, BioRad).
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bioRxiv - Microbiology 2023Quote: ... or a median 750 ng (for HIV-1) of genomic DNA was combined with ddPCR Supermix for Probes (no dUTPs, BioRad), primers (final concentration 900 nM ...
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bioRxiv - Immunology 2023Quote: The previously obtained plasma (1 µl) and gut mucus (30 µl) were separated on a 4-15% Mini-PROTEAN TGX gel (BioRad) under non-reducing conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... PVDF membranes were blocked for 1 hr in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4°C with antibodies listed in Table S1 in 5% BSA in TBS-T ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins of the parasite pellets were extracted using lysis buffer (1 mM DTT, 2X Laemmli buffer (Bio-rad, 161-0737) and protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2023Quote: ... in 1× TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (BioRad). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplification was performed in the presence of 1 mM of each oligonucleotide and iTaq Universal SYBR Green Supermix (Bio-Rad). Relative amounts of targets were calculated using the ddCT method ...
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bioRxiv - Immunology 2023Quote: ... Resultant cDNA at 1:100 dilution was subject to qPCR performed using iQ SYBR Green Supermix (BioRad, Cat. No. 1708880) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied to an equilibrated 1-ml StrepTrap HP chromatography column connected to the NGC Chromatography System (BioRad), washed with 20 CV of StrepA and elution was performed by applying a gradient concentration of StrepB (150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared assay plate was exposed to temperature increases from 25 ⁰C to 95 ⁰C with 1 ⁰C increments for 5 s each using a CFX Connect Real-Time PCR Detection System (BioRad). SYBR fluorescence was detected after every temperature increase ...
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bioRxiv - Molecular Biology 2023Quote: ... Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad). The loaded resin was then washed 3-4 times with 6 ml Wash Buffer (50 mM sodium phosphate ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature prior to development with clarity ECL (BioRad) developing reagents with a ChemiDoc Touch (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... After 3x 10 min washes in TBST appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature and developed with a ChemiDoc Touch (BioRad) as described above.
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bioRxiv - Cell Biology 2023Quote: ... the membrane was cut lengthwise into strips to isolate bands of particular molecular weights before being washed with TBST and incubated at room temperature for 1 H in a 5% blocking buffer solution (Biorad). The western blot strips were then incubated overnight at 4 C in a solution consisting of the primary antibody diluted into blocking buffer ...
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bioRxiv - Biophysics 2023Quote: ... Labeling efficiency was determined by measuring the absorbance of pyrene (χ346nm = 42,000 M-1cm-1) and protein concentration by 660 nm assay (Bio-Rad). An SDS-PAGE shift assay for isolating single-mSA bound ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated 1 h with horseradish peroxidase-conjugated secondary antibodies (1:5,000) and the bands were visualized by chemiluminescence using the Clarity Western ECL substrate (Bio-Rad) and a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... was added to the reactions and the products were separated on 4% polyacrylamide gels (ratio acrylamide:bisacrylamide 19:1, Bio-Rad) in TAE (40 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... Recovered DNA suspended in molecular grade water was used for Sybr Green reactions (1 µL) on a BioRad CFX384 (BioRad). ChIP-qPCR primers (50-150 bp ...
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bioRxiv - Microbiology 2023Quote: The cDNA was diluted to 1:20 and analysed using iQ5 Real time PCR detection system (BioRad, Hercules, California, USA) with SYBR Green detection system ...
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bioRxiv - Molecular Biology 2023Quote: ... These mixtures were then heated up to 95 °C for 5 minutes and slowly cooled to room temperature at the rate of 1 °C per minute using a thermal cycler (BioRad).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBS-T (3 × 5 minutes) and incubated in horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (BioRad) in blocking solution for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed on a 1% agarose gel using VersaDoc 3000 Gel Imaging System (Bio-Rad, Hercules, CA, USA). The intensity of the bands was quantified by densitometric analysis using ImageJ 1.53k software (NIH ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the nitrocellulose membrane was incubated with ECL reagent SignalFire for 1-2 min and imaged using a ChemiDoc (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... 95°C for 2 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min (BioRad). The following primers from Integrated DNA Technologies (IDT ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Cancer Biology 2023Quote: ... qPCR duplicates were carried out using 2 µL of diluted cDNA (1 into 100) in a 20 µL-reaction using Sso Fast EvaGreen reagent (BioRad) following supplier’s instructions on a StepOne Plus™ Real-Time PCR system (Applied BioSystems) ...
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bioRxiv - Genetics 2022Quote: ... We washed the membrane 3× 5 minutes in TBS-T and 1× 5 minutes TBS before imaging (Bio-Rad ChemiDoc).
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bioRxiv - Cancer Biology 2022Quote: ... Blots were rinsed and probed in the appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (1:10,000; BioRad, Hercules, CA, USA) for 30 min at RT in 2.5% NFDM or BSA in 1× TBST ...
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bioRxiv - Cell Biology 2023Quote: ... phosphorylated in vitro by PLK-1 or CyclinB-Cdk1 as described (7) were separated on stain Free SDS-PAGE 10% gel (Biorad). The gel was imaged and then transferred to a PVDF membrane 0.45 µm during 1h30 at 90V ...
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bioRxiv - Microbiology 2023Quote: ... This step was followed immediately by an hour incubation with 1:5000 dilution of Goat α-mouse-HRP (Bio-Rad) in TBST ...
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bioRxiv - Biochemistry 2023Quote: For VP protein analysis 3×109-1×1010 vg of purified AAV were mixed with 12.5 µl 4x Laemmli Sample Buffer (Bio-Rad, supplemented with 10% 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR reactions were prepared from cDNA at 1:5 dilution with SYBR Green iTaq Universal SYBR Green Supermix (Biorad, 1725120) and qPCR primers at 10 µM in a total volume of 10 µL in a ≥ 40-cycle and melt curve reaction cycler (Biorad ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was incubated with anti-G50 antiserum (1:3,000 dilution) and horseradish peroxidase–linked anti-rabbit immunoglobulin (Bio-Rad Laboratories Inc. ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with horseradish-peroxidase-linked secondary antibodies diluted 1:5000 followed by exposure to Clarity Western ECL Substrate (Cat. No. 170-5060, BioRad) and film development ...
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bioRxiv - Cancer Biology 2023Quote: ... were thawed on ice for 20 min prior to addition of 1 μL of Gibson reaction and electroporation (1800 V) in a MicroPulser Electroporator (Biorad). 975 μL of pre-warmed recovery medium were added and the bacteria were incubated 37 °C at 250 rpm for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed on 1 µg of purified RNA using the iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA). For each quantitative real-time PCR reaction ...