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Citations for Bio-Rad :
7601 - 7650 of 7957 citations for Myeloperoxidase MPO Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL re-suspended Fraction B pellets were run 1 cm onto an Any kD Mini-Protean TGX gel (Bio-Rad) followed by Coomassie Blue staining ...
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bioRxiv - Neuroscience 2022Quote: ... First-strand cDNA synthesis was performed from 1 μg of RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad). Primers were designed using the NCBI Primer-BLAST tool and supplied by Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted 1:50 in water and subjected to RT-qPCR using the CFX384 Touch Real-Time PCR Detection System (BIO-RAD) and the ORA-qPCR Green ROX L 2X Mix (HighQu ...
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated with anti-mouse or –rabbit secondary antibodies tagged with horseradish peroxidase (1/10 000, Bio-Rad Laboratories) for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent ...
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bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of diluted (1:8) cDNA were loaded for qPCR analysis along with the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725272) and 0.5 μM forward and reverse primers for target genes (listed below) ...
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bioRxiv - Plant Biology 2022Quote: ... The dicing reactions were performed at 37 °C for 30 min and stopped by adding 1 volume TBE-Urea sample buffer (Bio-Rad), heating at 95 °C for 10 min and quickly chilling on ice for 2 min ...
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bioRxiv - Neuroscience 2022Quote: ... Single cell suspension was stored on ice in 1X EBSS+1%BSA while the live cell count/sample was determined using a TC20 Automated Cell Counter (Bio-Rad). For the extraction of metabolites 1x10^6 cells/sample were transferred into a 1.5ml Eppendorf tube ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cells were physically disrupted by adding 500 µL of 25:24:1 solution of phenol:chloroform:isoamyl alcohol and homogenizing in a bead beater (TeSeE Precess 48 Homogenizer, Bio-Rad, Hercules, CA) for 90 sec at 5300 rpm ...
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bioRxiv - Synthetic Biology 2024Quote: The samples (1 µL) were subjected to SDS-PAGE and transferred to a nitro cellulose membrane (Bio-Rad Laboratories, CA, USA). The membrane was blocked using 3% skim milk in PBST (137 mM NaCl ...
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bioRxiv - Plant Biology 2024Quote: pTRV1 and pTRV2-Del/Ros-1 harboring the SlATG7- or SlATG2-derived fragments were introduced into Agrobacterium strain GV3101 using electroporation (BIO-RAD). A 5 mL culture was grown overnight at 28°C in a medium containing kanamycin ...
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bioRxiv - Physiology 2024Quote: ... was used to wash the membranes before incubating with goat anti-rabbit horseradish peroxidase conjugated secondary antibody at 1:5000 dilution (1706515, Bio-Rad) for 90 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were mixed 1:6 with a loading dye and run on an agarose gel (1%) in Tris-acetate-EDTA buffer supplemented with 0.2 µG/mL of Ethidium Bromide (Biorad, Hercules, California). DNA was visualized under a short-wavelength UV light to confirm the lack of amplification for Heat-Killed Microbiome PMA-treated in comparison to the Heat-Killed Microbiome control (Supplementary Figure 2).
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bioRxiv - Pathology 2023Quote: ... Signal was visualized using a secondary horseradish-peroxidase-labeled goat anti-mouse antibody (goat anti-mouse IgG-HRP 1:5000, SantaCruzBiotechnology) and enhanced chemiluminescence (ECL Clarity Bio-Rad). The purity as well as equal loading (40γ ...
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bioRxiv - Microbiology 2024Quote: ... 1−10 ng of sample DNA was used for qPCR using a MiniOpticon qPCR detection system (Bio-Rad Laboratories, Hercules, USA). The iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodisc formation was initiated by adding washed Bio-Beads (200 mg dry weight per 1 mL of the protein mixture; Bio-Rad), and the mixture was rotated for two hours at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: Proteins from SDS-PAGE gels were transferred onto a PVDF (polyvinylidene difluoride) membrane (pre-activated in methanol for 1 min) using Trans-Blot Turbo transfer system (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... then incubated with ProSignal Pico Spray chemiluminescence substrate (Prometheus) for 1-3 minutes at room temperature and imaged using a ChemiDoc Imaging System (Bio-Rad). Contrast was occasionally adjusted to improve visualization of bands ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µL of each sucrose gradient fraction was incubated at 95 ºC with 1 % sodium dodecyl sulfate (SDS) and applied for SDS-PAGE with a 4-20 % gradient SDS-PAGE gel (Bio-rad). The proteins were transferred to a nitrocellulose membrane (Cytiva ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... All the reactants were run on 4.5% non-denaturing PAGE in 1× TBE and then Cy3 or Cy5 fluorescence signal was imaged by ChemiDoc MP imaging system (Bio-Rad). S8 Table presents details of the oligonucleotide sequence used for EMSA.
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4 °C and subsequently washed in TBST and incubated for 1 hours at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... EtOH (p.a.)) and the Bio-Rad trans-blot® TurboTM Trans System (25 V, 0.5-1 A, 25 min; Bio-Rad, Germany). Afterward ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were then stained with Coomassie Brilliant Blue G (80 mg dissolved in 1 L water with 30 mM HCl) and destained with water before visualising using a ChemiDoc MP Imaging System (Bio-Rad). For mNeonTP-loaded encapsulins ...
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bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
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bioRxiv - Microbiology 2024Quote: ... using a Superdex 30 Increase 10/300 GL (Cytiva) equilibrated with 50 mL water operated at 500 µL min−1 on an NGC medium-pressure chromatography system (Bio-Rad). Per chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed thrice and incubated with a secondary antibody (Goat anti-Mouse IgG-HRP, 1706516, Bio-Rad Laboratories, 1:10K), also diluted in 2.5% milk in TBST ...
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bioRxiv - Bioengineering 2023Quote: ... For detection, we used an anti-rabbit HRP-coupled secondary antibody (1:5000, Cell Signalling) and Western ECL substrate (#1705061, Bio-Rad. As a loading control ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed then incubated for 1 hour at room temperature with secondary antibody (Goat Anti-Mouse Starbright Blue 520 and Goat Anti-Rabbit Starbright Blue 700, each at 1:2500, Bio-Rad). Blots were analyzed (ChemiDocTM MP Imaging System ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then separated by 20% Urea-PAGE gel at 250 V for 1 h and the fluorescent signal was captured by the ChemiDoc System (Bio-Rad). For protein detection ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... the membrane was washed with 1X PBS and thereafter incubated with HRP-conjugated secondary antibody for 1 hour at room temperature and detected using ECL reagent (BioRad, USA) under chemiluminescence ...
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bioRxiv - Biochemistry 2024Quote: Rabbit RyR1 was purified following a previously described procedure 55 and reconstituted into amphipol A8-35 by mixing at 1:10 (w/w) with RyR1 followed by overnight detergent removal with BioBeads (Bio-Rad). For the Nb selection ...
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bioRxiv - Biophysics 2024Quote: ... containing 20 mM MgCl2 and exposed to a thermal annealing ramp (65 °C, 15’; 58-53 °C, 1 h/°C; stored at 20 °C) in a Tetrad2 (Bio-Rad) thermocycler ...
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bioRxiv - Molecular Biology 2023Quote: ... blots were incubated with secondary antibodies for 1 h and were consequently developed using Clarity Western ECL and Clarity Max Western ECL substrates (Bio-Rad). Protein bands were visualized on the ChemidocTM MP imaging system (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... First strand cDNA samples were diluted 1:10 and 5 μL was used as template with ddPCR Supermix for Probes (No dUTP) (Bio-Rad) and TaqMan Gene Expression Assays (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Appropriate secondary HRP-linked antibody was incubated for 1 hour at room temperature and developed using Clarity Western ECL blotting substrate (BioRad, 1705061).
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bioRxiv - Cell Biology 2023Quote: ... such that a fluorescent construct can be used as an expression reporter for cells co-transfected with a non-fluorescent construct.1 Cells were electroporated at 280 V and 950 μF using Gene Pulser X (Bio-Rad), then immediately resuspended in 6 ml medium and cultured in MatTek dishes (2 ml/dish ...
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: ... the membranes were washed with TBS and incubated for 1 hour at room temperature with the secondary antibody (HRP-conjugated goat antirabbit or goat antimouse antibody) (Bio-Rad). Protein bands were visualized and quantified using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were run at 4V for 16 h with a 1 – 10 running ratio in TBE buffer using a CHEF-DRII system (Bio-Rad). The gel was stained by 0.005% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplification products were analysed on 1% agarose gel (80V for 1 hr) stained with ethidium bromide (10 mg/mL) and viewed on Gel Doc XR Imaging System (BioRad, USA).
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bioRxiv - Molecular Biology 2023Quote: ... containing β-mercaptoethanol (1/10 in 4X buffer) and transfer of protein onto PVDF or nitrocellulose using the Trans-Blot Turbo System (Bio-Rad). Secondary HRP-conjugates were visualized after addition of ECL Prime Western Blotting Substrate and chemiluminescent detection in an Amersham Imager 680 or fluorescence detection using a LICOR Odyssey CLx ...
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bioRxiv - Microbiology 2023Quote: ... 2% formaldehyde gel and separated at 125-150V at 4°C for 1-2 h and then transferred to a Zeta-Probe GT membrane (Bio-Rad) via capillary action overnight (Streit et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) was carried out using 4 μl cDNA (diluted 1:20) with iQ SYBR Green Supermix (BioRad, CA, USA) and specific primers (Biolegio ...
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bioRxiv - Microbiology 2023Quote: ... Amplicons were visualized in 1% agarose gels stained with ethidium bromide using a ChemiDoc Imaging System (Bio-Rad Laboratories, Hercules, CA). We then sent amplicons to the Génome Québec Innovation Centre (McGill University ...
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bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of protein extracts were run at 100V for 1 hr on 10% pre-cast SDS-PAGE gels (Bio-Rad), then electro-transferred to 0.2 μm nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... were used; and as secondary antibody HRP-conjugated anti-rabbit or anti-mouse (both 1:10 000; anti-rabbit (1706515; Bio-Rad); anti-mouse (170-6516 ...