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Citations for Bio-Rad :
7101 - 7150 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Reverse transcription (RT) was performed on 1 μg of DNase-treated total RNA using the iScript cDNA synthesis kit (Bio-Rad #1708890), with the same amount of total RNA diluted in water as negative controls for RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... The product from the final elution and whole cell lysate supernatant were subjected to silver staining using Silver Stain Plus Kit (BIO-RAD, # 1610449) to check pull down efficiency and purity ...
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bioRxiv - Immunology 2021Quote: ... 30 μg of protein was mixed with an equal volume of 2×Laemmli buffer supplemented with 5% 2-mercaptoethanol (Catalog no. 1610737) then loaded in 10% polyacrylamide precast gel (TGX™ FastCast™ Acrylamide Starter Kit, 10%, catalog no. 1610172) from BioRad Laboratories (Mississauga ...
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bioRxiv - Molecular Biology 2021Quote: ... and quantitative PCR (qPCR) was performed to quantify mRNA abundance using a SsoFast™ EvaGreen® Supermix kit (Bio-Rad, 172-5204,) on a Bio-Rad CFX96 cycler ...
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bioRxiv - Immunology 2020Quote: ... Multiplexed cytokine measurements on macrophage cell culture supernatants were performed using a custom Bio-Plex Express Human Cytokine kit (Bio-Rad 17004073) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 30 µg of protein was mixed with an equal volume of 2×Laemmli buffer supplemented with 5% 2-mercaptoethanol (Catalog no. 1610737) then loaded in 10% polyacrylamide precast gel (TGX™ FastCast™ Acrylamide Starter Kit, 10%, Catalog no. 1610172) from BioRad Laboratories (Mississauga ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Reverse transcription reactions were performed to synthesize cDNA from 200 ng of total RNA using the iScript™ Select cDNA Synthesis Kit (Biorad, CA). Real-time quantitative PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were stained using the Silver Staining Plus Kit and documented on the ChemiDoc XRS+ system (Bio-Rad Laboratories Inc., Hercules, USA) according to the manufacturers protocol.
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bioRxiv - Genomics 2020Quote: ... the membrane was incubated with peroxidase-conjugated goat anti-mouse IgG and proteins were detected using a chemiluminescence detection kit (Super-Signal West Pico, Pierce) and a ChemiDoc™ MP system (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad, Marnes-la-Coquette, France). The membranes were blocked for 30□min in 5% low-fat milk in TBS-0.1% Tween 20 at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The immunoblotting signals were detected using a CCD camera (Intas Biopharmaceuticals, Ahmedabad, India) after application of enhanced chemiluminescence detection kit (Clarity™ Western ECL Substrate; Bio-Rad, Hercules ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR analysis were performed with iQ-SYBR Green Supermix kit in an iCycler iQ5 system following the instructions of the manufacturer (Bio-Rad, USA). The relative quantifications of mRNA levels were performed using the cycle threshold (Ct ...
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bioRxiv - Synthetic Biology 2022Quote: ... #FABRK 001-1) and 400 ng of cDNA was synthesized using the iScript™ Select cDNA Synthesis Kit (Bio-Rad Laboratories, #1708897) on a Mastercycler® nexus PCR Thermal Cycler (Eppendorf) ...
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Germany) and 1 μg of total mRNA was translated to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according to the instructions.
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bioRxiv - Neuroscience 2022Quote: ... 500 ng of total RNA was used in a 20 µL reaction volume with the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, #1708890) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein measurements were performed using a BCA kit (Pierce) and 150 µg was loaded on a 10-20 % tris/tricine SDS-page gels (Bio-Rad Laboratories) and blotted onto 0,2 µm nitrocellulose membrane (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using gDNA of purified viruses was performed in parallel of each experiment ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription of the RNA was performed to synthesize cDNA following the standard manufacturer’s instructions of the iScript Reverse Transcription Supermix for RT-qPCR kit (Bio-Rad PN 1708841). cDNA was submitted to the University of North Carolina Chapel Hill Advanced Analytics Core (UNCAAC ...
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bioRxiv - Neuroscience 2024Quote: ... The signals were detected using an enhanced chemiluminescent (ECL) plus reagent kit (Share-Bio, Cat. SB-WB012L, China) and viewed with a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Zoology 2024Quote: ... and 100 ng of RNA were used for cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA), resulting in a final concentration of 5 ng/μl for each sample.
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bioRxiv - Microbiology 2023Quote: ... the fluorescence derived from the incorporation of SybrGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoAdvanced Universal SYBR Green Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Bioengineering 2024Quote: Four samples of total RNA were collected for each treatment condition using the Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of RNA was taken to synthesize complementary DNA (cDNA) conversion reactions were done using an iScript™ Advanced cDNA Synthesis Kit (catalog no. 18080085, Biorad, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers/probe mixtures (all from Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: cDNA was synthesized by reverse transcription from 500 ng of total RNA according to manufacturer’s instructions (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). Real-time PCR reactions using primers hybridizing with ERG25 or ACT1 transcripts were performed in an CFX96TM Real-Time System C100TM Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using the gDNA of purified viruses was performed in parallel with each experiment ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was conducted using HiScript II One Step qRT-PCR SYBR Green Kit (Q221-01, Vazyme) with gene-specific primer pairs on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories). The thermal cycling protocol was as follow ...
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bioRxiv - Bioengineering 2024Quote: RNA was diluted in nuclease-free water and combined with the reagents from the One Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, USA) and eGFP primer/probe set with final primer concentrations of 900 nM and probe concentrations of 250 nM to measure transgene expression ...
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bioRxiv - Molecular Biology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, USA). The detection limit was calculated to be 1000 copies per reaction ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Physiology 2023Quote: ... The cDNA was obtained from equal amounts of total RNA by reverse transcription using an iScriptTM cDNA Synthesis Kit (Bio-Rad, CA). Gene expression of 96 fibrosis-associated genes were evaluated using a PCR array kit (RT² Profiler™ PCR Array Rabbit Fibrosis PANZ-120Z ...
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bioRxiv - Plant Biology 2023Quote: ... The 1st strand cDNA was synthesised from 1 µg of RNA for each sample using iScript™ cDNA Synthesis Kit (Bio-Rad). This cDNA was then used as the template in qRT-PCR reaction mixture after 10 times dilution ...
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bioRxiv - Microbiology 2023Quote: ... modified from previously published primers/probe (44)) was performed using the BioRad iTaq Universal Probes One-Step Kit (BioRad, Cat. No. 1725140). Thermocycling was performed on a BioRad CFX Connect Real-Time PCR Detection System (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) synthesis was performed using 1 μg of total RNA with the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The genes encoding for the zonula occludens-1 (Zo-1) ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized with 1 µg of total RNA by using the iScript cDNA Synthesis Kit (Bio-Rad, Cat# 1708891). cDNA was diluted 20 times and real-time quantitative PCR was performed by using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Visualization was performed using a horseradish peroxidase sensitive ECL western blotting detection kit (SuperSignal West Pico PLUS Chemiluminescent Substrate, ThermoScientific) and the result was recorded using Chemidoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... Membranes were developed using the SuperSignal West Pico Chemiluminescent Substrate kit (Fisher #PI34578) and visualized on a ChemiDoc™ Touch Gel Imaging System (BioRad #1708370).
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bioRxiv - Bioengineering 2023Quote: ... We then added 80 μL of X1 streptavidin phycoerythrin SA-PE (Bio-Plex Pro Reagent Kit III #171304090M, BioRad, Hercules, CA, USA) and incubated it for another 20 minutes ...
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bioRxiv - Physiology 2023Quote: ... One hundred nanograms of RNA was used as input to synthesize cDNA using the iScript cDNA synthesis kit (BioRad, Hercules, CA, USA). cDNA templates were used for quantitative real-time PCR ...
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bioRxiv - Bioengineering 2023Quote: ... After RNA isolation the respective complementary DNA (cDNA) was then synthesized using the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, USA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... First-strand cDNA was synthesized from 200 ng of total RNA in a 20 μl reaction using the iScript™ cDNA Synthesis Kit (Cat#: 1708891, Bio-Rad). A SYBR green real-time PCR kit (Cat# 001752A ...
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bioRxiv - Pathology 2023Quote: ... Two μg of total RNA from each sample were used for cDNA synthesis using the iScript Advanced cDNA Synthesis kit (Bio-Rad, CA).
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bioRxiv - Immunology 2023Quote: ... Proteins in the gels were transferred to the PVDF membrane using the Trans-Blot Turbo Kit by the Trans-Blot Turbo transfer system (Bio-Rad, 1704156). Membranes were then incubated in a blocking buffer (Nacalai Tesque ...
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bioRxiv - Immunology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Microbiology 2024Quote: qRT-PCR analysis was performed on three major selected genes and validated as follows: The first cDNA strand was prepared using the iScript™ cDNA synthesis kit (Bio-Rad). qRT-PCR was performed using the SsoFast™ EvaGreen® supermix kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... CD45-APC and Bu1-PerCP-Cy5.5 were generated by conjugation of the antibody with the respective Lynx Rapid conjugation kit (Bio-Rad, Feldkirchen, Germany). Samples were analyzed on a FACS Canto II and FlowJo 10.9 software (BD ...
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Developmental Biology 2024Quote: ... using cDNA generated from 0.1-2 ng total RNA with the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad catalog # 1864021) according to the manufacturer’s protocol with reverse transcription at 42°C and annealing/extension at 60°C.