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Citations for Bio-Rad :
6951 - 7000 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and unbound RNA on a Hybond-XL (Cytiva) nylon membrane with a Bio- Dot apparatus (Bio-Rad, Hercules, CA). After applying the sample under vacuum ...
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bioRxiv - Plant Biology 2024Quote: ... protein samples were separated on standard SDS-PAGE (12% acrylamide) gel and subsequently transferred to PVDF membrane (Bio-Rad) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... The protein from the gel was transferred to a 0.45 μm or 0.22 μm nitrocellulose membrane (Bio-Rad #1620115) using the Bio-Rad transferring system ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated by SDS 12% PAGE and transferred to polyvinylidene fluoride membranes (Bio-Rad, Marnes-la-Coquette, France) blocked with 5% bovine serum albumin ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to 0.2 mm nitrocellulose membranes using semi-dry transfer (Bio-Rad Transblot Turbo; 25V 30 min) and visualized by Coomassie stain (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were resolved by SDS-PAGE then transferred onto nitrocellulose membranes using a wet tank transfer system (Bio-Rad). Membranes were blocked in 5% milk in TBST and probed overnight at 4 °C with the appropriate antibody diluted in 5% BSA in TBST ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto a nitrocellulose membrane using the Trans-Blot system (Bio-rad) according to the manufacter’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and transferred to a PVDF membrane using the protocol mix molecular weight of the Trans-Blot Turbo System (BioRad). The membrane was blocked with 5% milk in TBS-T (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... The separated proteins were then transferred onto nitrocellulose membrane using a Trans Blot Mini Cell (Bio-Rad Cat# 1703930) at 100V for 1h ...
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bioRxiv - Genomics 2024Quote: ... membranes were washed and incubated with HRP-conjugated secondary antibodies (goat anti-mouse or goat anti-rabbit, Bio-Rad). Protein expression was detected using an ECL detection kit (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Transfer to low-fluorescence PVDF membranes was performed with the Trans-Blot Turbo Transfer System (both from Bio-Rad).
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bioRxiv - Biophysics 2024Quote: ... Proteins were separated on 12% SDS-PAGE polyacrylamide gels and transferred to Polyvinylidene fluoride (PVDF) membranes (Bio-Rad Laboratories). The membranes were blocked using a blocking buffer Tris-buffered saline containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Cell Biology 2024Quote: ... with further transfer using precast Trans-Blot Turbo Mini 0.2 µm Nitrocellulose membrane using Trans-Blot Turbo semi-dry blotting system (BioRad) according to manufacturer recommendation.
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bioRxiv - Cancer Biology 2024Quote: ... 20lilµg of total protein from each sample were subjected to 7.5% or 10% SDS polyacrylamide gel electrophoresis (PAGE) and electrophoretic transfer onto 0.2lilµm polyvinylidene difluoride (PVDF) membrane (Bio-Rad) at 30lilV ...
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bioRxiv - Biochemistry 2024Quote: ... then transferred onto nitrocellulose or PVDF membranes (in the case of LC3 blotting) using the TransBlot Turbo system (BioRad). Membranes were blocked in 5% milk in PBS with 0.001% Tween-20 (PBST ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were loaded at same amount by SDS-PAGE and transferred to 0.22 μm Immuno-Blot PVDF membranes (BIORAD). Membranes were then blocked in 5% milk/TBST for 1 h at room temperature and incubated with primary antibodies at 4 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... the sample was transferred from the SDS-PAGE to a blotting membrane (Trans-Blot Turbo Transfer Pack, Bio-rad) using the ‘Mixed MW’ program of the Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Biochemistry 2024Quote: ... and transferred to a PVDF membrane using the protocol “mix molecular weight” of the Trans-Blot Turbo System (BioRad). Membranes were blocked with 5 % milk in TBS-T (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were then fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to PVDF membranes (Biorad). Membranes were blocked in 4% BSA solution for 1 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... the same amount of protein was loaded on SDS-PAGE gels and then electrotransferred onto PVDF membranes (Bio-Rad). After blocking for 1 hour with 5% BSA and incubating for 1 hour with primary antibody ...
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bioRxiv - Bioengineering 2024Quote: ... The proteins were electrotransferred to a polyvinylidene fluoride membrane (sigma) using an Trans-Blot Turbo Transfer System (Bio-Rad). The membranes were blocked with 5% nonfat milk ...
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bioRxiv - Cell Biology 2024Quote: ... 20/25 ug total protein was then loaded into an 8% polyacrylamide SDS-Page gel and then transferred to a pre-assembled PVDF membrane (Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs, BioRad) using a semi-dry method (BioRad) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto nitrocellulose blotting membrane (Amersham, Cytiva) in a Mini Trans-blot ® cell system (Bio-Rad). After transfer ...
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bioRxiv - Cell Biology 2024Quote: ... followed by transfer onto 0.45 µm or 0.2 µm nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were quickly washed with distilled water and blocked with 5% milk in 0.1% TBS-Tween for 1 hour at room temperature ...
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bioRxiv - Genetics 2024Quote: ... Densitometric measurements for all blots was performed using Image Lab following development of blot membranes with Dura ECL reagent (Thermo) using a GelDoc imaging system installed with software version 5.1 (BioRad). For Phospho-RRXS/T blots the entire lane was analysed using a band detection threshold of 95% and a cumulative densitometry was calculated ...
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration of the resulting supernatant was determined by the Bradford protein assay (Bio-Rad). Extract volumes containing 200 micrograms of protein was added to 500 microlitres cold buffer AE (50mM sodium acetate pH 5.3 and 10mM ethylenediaminetetraacetic acid ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was collected and used to determine protein concentration using a Bradford Assay (Bio-Rad), and samples were prepared with 4x Laemmli Sample Buffer (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentration of samples was determined using the DC (detergent compatible) protein assay (Bio-Rad, #5000112). Samples were prepared in a total volume of 20 μL at 15 μg/μL in Laemmli loading buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was quantified with the Bradford assay (cat n. 5000001, Bio-Rad Laboratories, Segrate, Italy). Proteins ...
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bioRxiv - Cell Biology 2020Quote: ... BCA assays were performed where necessary to determine protein concentration according to the manufacturer’s instructions (BioRad). Samples were boiled in 1x LDS sample buffer (Life Technologies ...
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bioRxiv - Genetics 2021Quote: ... The protein was quantified using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 5000006).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were collected and protein concentrations were determined using Bradford protein assay (Bio-Rad 5000006). Lysates were then mixed with SDS loading buffer and denatured at 95 °C before fractionation by SDS-PAGE and analyses by western blot ...
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bioRxiv - Neuroscience 2021Quote: Sample protein concentrations were measured using Bio-Rad DC protein assay (Bio-Rad Laboratories, Hercules, CA). Proteins (40 μg ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations were determined using the Bio-Rad protein assay (Bio-Rad Laboratories Inc., Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... according to standard parameters for real-time RT-qPCR assays (Bio-Rad Laboratories, Hercules, CA, U.S.A.). For qRT-PCR experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was measured via the Braford method with Protein Assay Dye from BioRad (Hercules, CA). Ten to 50 µg protein was loaded into a 12% SDS-PAGE gel ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration of the supernatant was determined by Quick Start TM Bradford assay (Bio-Rad) following standard protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Final qPCR was performed using TLR4 (qMmuCIP0035732) and HPRT (qMmuCEP0054164) primer-probe assays obtained from BioRad and the associated SSO Advanced Universal Probe Super Mix kit (BioRad 1725281).
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bioRxiv - Cancer Biology 2020Quote: ... lysate concentrations were adjusted to equal protein concentrations using the bicinchoninic acid (BCA) protein assay (Biorad). Equal amounts of protein were mixed to a final concentration of 1x reducing sample buffer (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... were determined using Bio-Plex Pro TGF-β 3-Plex Assay (171W4001M) (Bio-Rad, Hercules, CA).
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bioRxiv - Genetics 2020Quote: Digital droplet PCR (ddPCR) assays were performed on a QX200 Droplet Digital PCR System (Bio-Rad) according to the method detailed in the Bio-Rad Droplet Digital™ PCR Applications Guide essentially as described before (27) ...
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bioRxiv - Neuroscience 2020Quote: Protein concentrations were determined using the Bio-Rad protein assay system (Bio-Rad Laboratories, Richmond, CA). Equal amounts of protein were resolved by SDS-PAGE using a 10 % acrylamide gel and subsequently transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Genomics 2021Quote: Droplet digital PCR (ddPCR) was conducted on a QX200 instrument with the Evagreen assay reaction (Biorad). Copy number measurements from specific TE primers (Table S3 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration in each sample was determined using the Bradford assays (Bio-Rad, Munich, Germany).
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bioRxiv - Systems Biology 2021Quote: ... Concentration determination was carried out by standard Bradford method (Bio-Rad Protein Assay Dye Reagent #5000006) using BSA (MP BIomedicals #160069 ...
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bioRxiv - Neuroscience 2020Quote: ... the soluble fraction from 3 months old APPPS1 mice have been quantified using Bradford assay (Biorad) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration was determined using the Bradford protein dye binding assay (#5000113 + #5000114, Bio-Rad). 50μg of protein was separated by electrophoresis in an SDS-polyacrylamide gel which was then transferred using the semi dry method ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration in each elution fraction was measured using protein assay dye reagent concentrate (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... and protease inhibitors and the protein content quantified using a detergent-compatible protein assay (Bio-Rad). Lysates (10 μg ...
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bioRxiv - Neuroscience 2021Quote: ... Metabolite levels were normalised to protein levels quantified with the Bio-Rad Protein Assay (Bio-Rad) on formate-resuspended pellets from the aforementioned HClO4 extraction.