Labshake search
Citations for Bio-Rad :
651 - 700 of 1856 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: Biotinylated and non-biotinylated protein antigens and OMVs were mixed with loading buffer containing β-mercaptoethanol and boiled for 10 min prior to loading onto Mini-PROTEAN TGX polyacrylamide gels (Bio-Rad). To determine protein purity ...
-
bioRxiv - Genetics 2021Quote: ... washed and transferred into droplets of Opti-MEM medium containing the previously prepared RNP complex and electroporated using a BioRad Gene Pulser (BioRad, Feldkirchen, Germany) (two 3ms square wave pulses at 30V with an 100ms interval) ...
-
bioRxiv - Microbiology 2019Quote: ... qPCR was carried out in 20 µL solutions containing 10.4 µL Sso Advanced SYBR green PCR buffer (Bio-Rad, Hercules, CA, USA), 0.4 µL of 10 mM primer ...
-
bioRxiv - Microbiology 2019Quote: ... Each PCR was performed in a total volume of 10 µL containing 5 µL of 2 × iQSYBR Green supermix (Bio-Rad, USA), 200 nM of each primer ...
-
Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... we separated beads from solution and transferred 44 μL of the supernatant (containing the DNA) into a new 96-well PCR plate (Bio-Rad HSP9631) for library preparation ...
-
bioRxiv - Molecular Biology 2021Quote: Amplification was carried out using PCR mixture-2 blue (AmpliSens, Russia) containing Taq-polymerase on a T100 Thermal Cycler (Bio-Rad, USA). Next ...
-
bioRxiv - Molecular Biology 2020Quote: ... QPCR was performed using Precision Melt Supermix containing EvaGreen dye (cat# 172-5110) using CFX96 Touch™ Real-Time PCR Detection System (BioRad, USA). Sequences of PCR primers are listed in Table.
-
bioRxiv - Immunology 2021Quote: ... buffer containing dithiothreitol (DTT) and resolved by polyacrylamide gel electrophoresis (SDS-PAGE) on a Criterion TGX precast gel (Any-KD; Bio-Rad Laboratories). Proteins were then transferred to a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Microbiology 2019Quote: ... All symbiont 16S rRNA gene qPCR assays used SYBR®Green reaction mixtures containing 12.5 μL iQ SYBR®Green Supermix (Bio-Rad), and optimized concentrations of 400 nM primer as well as 0.25 mg ml-1 BSA ...
-
bioRxiv - Molecular Biology 2019Quote: The optimized ddPCR reaction mix conditions retained were a final reaction volume of 20 µL containing: 1x ddPCR™ Supermix for Probes (No dUTP) (Bio-Rad), 900 nM Xf forward and reverse primers (XF-F and XF-R) ...
-
Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... Proteins were separated on SDS-containing 8 to 12 % polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories, Inc., Hercules, USA). After staining with primary and secondary antibodies ...
-
bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
-
bioRxiv - Cell Biology 2021Quote: ... except that blocking was done with phosphate-buffered saline plus 0.1% Triton X-100 (PBS-T) containing 5% nonfat dry milk (Biorad Blotting grade Blocker) and 10 mM NaN3 ...
-
bioRxiv - Molecular Biology 2019Quote: The optimized final reaction conditions were performed in a final volume of 10 µL containing 1X of SsoAdvanced™ Universal Probes Supermix (Bio-Rad), 575 nM of primers ...
-
bioRxiv - Plant Biology 2020Quote: ... or 1 μl (for the rest) of a 1/2 dilution of the cDNA was added to a reaction containing 1X of SsoFast EvaGreen (Bio-Rad, USA), 0.5 μM Forward and Reverse primers (Table 2) ...
-
bioRxiv - Plant Biology 2020Quote: ... we used 2 µl of a 1/5 dilution of the cDNA obtained as above in a reaction containing 5 µl of SsoFast EvaGreen (Bio-Rad, USA), 0.5 µl of Forward primer (10 µM ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
-
bioRxiv - Neuroscience 2022Quote: ... Blots were blocked for 1 h at room temperature (RT) in Tris-buffered saline with 0.05 % Tween-20 (TBST) containing 5 % blotting-grade dry milk (Biorad cat# 170-6404), incubated overnight with primary antibody (dilutions above mentioned ...
-
bioRxiv - Neuroscience 2021Quote: ... Plates containing the amplified droplets were loaded into the QX200 droplet reader and results were analysed using QuantaSoft software™ (Bio-Rad).
-
bioRxiv - Microbiology 2021Quote: ... Samples were electrophoretically resolved on gelatin-containing gels at 125 V for 110 min at 4 °C using a Power Pack supply (Bio-Rad, USA) Next ...
-
bioRxiv - Neuroscience 2022Quote: ... macrophages were plated in a 24-well plate containing cover slips and incubated with the Magic Red cathepsin B substrate (1/250) (Bio-Rad, ICT938) for 2h ...
-
bioRxiv - Immunology 2020Quote: ... Conjugate DNA was measured by qPCR amplification from 1 μL of lysate in a 10 μL reaction containing 5 μL of iTaq Universal SYBR Green Supermix (Bio-Rad, 1725125) and 5 pmol of each primer (Supplementary Table 1) ...
-
bioRxiv - Cell Biology 2022Quote: ... for 20 minutes treated with PBS containing 1 % w/v BSA and 0.1 % v/v Triton X-100 (Bio-Rad, München, Germany) for 30 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products were run on a 1% Agarose gel containing .08% ethidium bromide and visualized using the ChemiDoc imaging system (BioRad, Hercules, USA). PCR amplicons were sanger sequenced (Psomagen ...
-
bioRxiv - Cancer Biology 2023Quote: ... All samples were mixed with loading buffer containing SDS and β-mercaptoethanol and run on homemade 15% or commercial 4-15% gradient acrylamide gels (Bio-Rad). Semi-dry transfer was performed on a Trans-Blot Turbo transfer system (Bio-Rad) ...
-
bioRxiv - Neuroscience 2023Quote: ... cDNA was added to a reaction mix (20 μL final volume) containing gene-specific primers and SYBR Green Supermix (Bio-Rad, 1725271). All samples were run in duplicates in LightCycler 480 II (Roche) ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
-
bioRxiv - Microbiology 2023Quote: ... 20 µL reaction mixes containing 100 ng template RNA were added to a 96-well PCR plate (Bio-Rad Cat. No. MLL9601) and sealed with adhesive Microseal ‘B’ PCR Plate Sealing Film (Bio-Rad Cat ...
-
bioRxiv - Microbiology 2023Quote: ... Gels were loaded with samples containing 10 μg total protein before electrophoresis on a 16.5% Tris Tricine gel (Bio-Rad, Hercules, CA). Gels were stained with the Pierce Silver Stain kit (Thermo Fisher ...
-
bioRxiv - Biochemistry 2023Quote: ... buffer containing dithiothreitol (DTT) and resolved by polyacrylamide gel electrophoresis (SDS-PAGE) on a Criterion TGX precast gel (Any-KD; Bio-Rad Laboratories). Proteins were then transferred to a PVDF membrane (Bio-Rad Laboratories ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples containing 236 µg protein lysate in final 96 µL of Lysis Buffer using a Bradford protein concentration assay (Bio-Rad 5000006) were then prepared for subsequent reduction and alkylation for 25 min at room temperature by the simultaneous addition of 10X tris(2-carboxyethyl)phosphine hydrochloride (TCEP ...
-
bioRxiv - Biochemistry 2023Quote: ... The spun down lysates were measured for total protein using a BCA assay and 15 μg of protein was diluted in 2x Laemmli buffer containing β-mercapto ethanol (Bio-Rad) and heated to 95 °C for 5 mins ...
-
bioRxiv - Physiology 2023Quote: ... 0.03% bromophenol blue) containing 9% beta-mercaptoethanol and separated by Mini-Protean TGX gel 4 - 15% (BioRad, stain-free gels #4568084, 4561035) and blotted onto Amersham Hybond-P PVDF membrane ...
-
bioRxiv - Neuroscience 2023Quote: ... using the Bio-Rad Trans-Blot Turbo Transfer System with 20% Methanol-containing transblot turbo transfer buffer (Bio-Rad; catalog no. 10026938) for 10-15 minutes ...
-
bioRxiv - Bioengineering 2024Quote: The extent of biotinylation was estimated using densitometry by comparing the intensity of the streptavidin-containing band to the band of the non-streptavidin sample using GelDoc Image Lab Software (BioRad, Hercules, CA). Considering the extent of biotinylation ...
-
bioRxiv - Microbiology 2020Quote: ... Appropriate secondary-HRP conjugated antibodies were used with clarity ECL (BioRad) developing reagents ...
-
bioRxiv - Molecular Biology 2020Quote: ... After incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (BIO-RAD), proteins were visualized using ECL detection reagents (GE Healthcare) ...
-
bioRxiv - Neuroscience 2021Quote: ... The antibodies were rat anti-BrdU (1:500, MCA2060, Bio-Rad) or rabbit anti-GFAP (1:300 ...
-
bioRxiv - Neuroscience 2021Quote: ... anti-Rabbit IgG HRP-conjugated antibody 1:5000 (#170-5046 BioRad). First ...
-
bioRxiv - Molecular Biology 2020Quote: ... an anti-α-tubulin antibody (MCA77G) was purchased from Bio-Rad, and an anti-JNK1 antibody (sc-571 ...
-
bioRxiv - Molecular Biology 2021Quote: ... mouse-HRP and rabbit-HRP secondary antibodies were purchased from BioRad Laboratories (Hercules ...
-
Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... A secondary anti-rabbit IgG antibody conjugated to horseradish peroxidase (BioRad) was used at a 1:3,000 dilution in the same buffer ...
-
bioRxiv - Genomics 2020Quote: ... HRP-linked antibodies were visualized using Clarity Western ECL substrate (Biorad) and imaged with Fujifilm LAS-3000 Imager (Fujifilm).
-
bioRxiv - Immunology 2019Quote: ... For detection of primary antibodies anti-rabbit-HRP (170-6515, BioRad), and anti-mouse-HRP (170-6516 ...
-
bioRxiv - Biophysics 2019Quote: ... Mouse anti Tubulin beta 3 antibody from BioRad (Hercules, CA, US). We used a 6xHis-GFP-labeled truncated human kinesin-1 heavy chain construct (amino acids 1-560 ...
-
bioRxiv - Bioengineering 2020Quote: ... and anti-sheep antibodies: CD45-FITC and CD44-FITC (Bio-Rad). Cells were stained as per the manufacturers’ instructions and analysis was completed using an LSR II flow cytometer (BD Biosciences ...
-
bioRxiv - Bioengineering 2020Quote: ... A FITC-conjugated rat anti-mouse CD204 monoclonal antibody (Bio-Rad) was prepared 1:200 in DMEM or CDMEM ...
-
bioRxiv - Biochemistry 2021Quote: ... and appropriate Starbright Blue 700 secondary antibody (Bio-Rad, Hercules, CA) for 1 hr at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were: goat anti- mouse IgG2b-HRP (Biorad, M32407; RRID:AB_2536647).
-
bioRxiv - Cancer Biology 2020Quote: ... Fluorescence of secondary antibody was detected using the Chemidoc system (BioRad) and data analysed by Image lab software (BioRad) ...