Labshake search
Citations for Bio-Rad :
451 - 500 of 1856 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Secondary HRP-conjugated antibodies were purchased from Bio-Rad and used at 1:5,000 dilution ...
-
bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase-conjugated secondary antibodies were from Bio-Rad Laboratories and IRDye secondary antibodies were from LICOR Biosciences.
-
bioRxiv - Neuroscience 2023Quote: ... Anti-rabbit IgG HRP-conjugated Antibody (Bio-rad 51962504), Anti-mouse IgG HRP-conjugated Antibody (Bio-rad 5178-2504) ...
-
bioRxiv - Neuroscience 2022Quote: Primary antibodies used were as indicated: Vinculin (Bio-Rad Cat# MCA465GA ...
-
bioRxiv - Neuroscience 2022Quote: ... rat anti-MBP (1:250, Bio-Rad antibodies MCA409S), rabbit anti-TPPP/p25 (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... and StarBright Blue 520/700 secondary antibodies (Bio-Rad) were applied at 1:2,500 dilution in TBS for 1 h ...
-
bioRxiv - Microbiology 2023Quote: ... and an anti-His-HRP conjugate antibody (Bio-Rad) was used in a 1:4000 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies from Bio-Rad and enhanced chemiluminescence (ECL ...
-
bioRxiv - Plant Biology 2023Quote: ... anti-rabbit secondary antibodies (BIO-RAD, 1:5000 dilution) or Pierce™ HRP-conjugated Streptavidin (Thermo scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-rabbit horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) or anti-goat horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and/or fluorophore conjugated secondary antibodies (Biorad, 1:2,000), washed three times and imaged ...
-
bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
-
bioRxiv - Immunology 2023Quote: ... and anti-neutrophil cytoplasmic antibody (ANCA) slides (29419, Biorad) were removed from pouches and allowed to equilibrate at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... All HRP-conjugated secondary antibodies were purchased from BioRad. Membranes were visualized using ECL (Millipore) ...
-
bioRxiv - Neuroscience 2023Quote: ... rat monoclonal antibody against Cd68 (1:200, BioRad, MCA1957), rabbit polyclonal antibody against Map2 (1:200 ...
-
bioRxiv - Neuroscience 2023Quote: ... and HRP-conjugated rabbit secondary antibody (BioRad, Irvine, CA) were used at 0.4 μg/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... we used primary alpha-tubulin rat antibody (Bio-rad) diluted 1:200 in 1% BSA in PBS for 1.5 hours at room temperature and secondary antibody anti-rat Alexa Fluor 647 (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... Fluorescent secondary antibodies (1:5000; Bio-Rad, Hercules, CA) were used to visualize the membrane ...
-
bioRxiv - Developmental Biology 2021Quote: ... membranes were incubated in 1:1000 rabbit anti-HSP90 antibody (CST #4874) and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). Signal was revealed using Clarity Max™ Western ECL Substrate (Bio-Rad #170-5062) ...
-
bioRxiv - Developmental Biology 2021Quote: ... membranes were incubated in 1:1000 rabbit anti-HSP90 antibody (CST #4874) and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). Signal was revealed using Clarity Max™ Western ECL Substrate (Bio-Rad #170-5062) ...
-
bioRxiv - Plant Biology 2021Quote: ... The following day it was probed with 1:2000 anti-Myc primary antibody (rabbit polyclonal, Santa-Cruz, A14) and with 1:10000 mouse anti-rabbit HRP linked secondary antibody (Bio-Rad, 170-6515). Amersham ECL prime HRP substrate (GE-Lifesciences ...
-
bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4°C and for 1 h at room temperature (RT) with secondary horseradish peroxidase conjugated antibodies (Bio-Rad, 1:3000). Membranes were then developed using the Clarity Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Immunology 2022Quote: ELISA plates were coated with 50 µL/well of 5 µg/mL of mouse anti-bovine IFN-γ monoclonal antibody (mAb) (CC330, Bio-Rad Antibodies) by overnight incubation at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
-
bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate samples containing 4 μg of protein were fractionated using a 4–20% SDS-PAGE gel (Bio-Rad). Proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo transfer system (Bio-Rad ...
-
bioRxiv - Molecular Biology 2021Quote: ... for 24 hours before being lysed in RIPA buffer containing protease inhibitors and protein concentration assessed using RcDc assay (BioRad). In some experiments cells were treated with 2μM of VX-445 (Selleckchem) ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were centrifuged for 5 min at 7,000 g and washed twice with 200 μL of 1x PBS containing 3% BSA and 0.1% (v/v) Tween-20 (BioRad, Germany). Mounting of cells for STED microscopy was performed as described above.
-
bioRxiv - Immunology 2019Quote: ... Real-time PCR was performed using 90 well-plates with 50-100 ng of cDNA per well containing the SsoFast EvaGreen Supermix (Biorad) and gene-specific primers ...
-
bioRxiv - Microbiology 2019Quote: Approximately 1 × 105 bacterial cells were suspended in PBS containing 1% (w/v) PBS-BSA and incubated with fH (Biorad) at a final concentration of 5 μg/mL for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2019Quote: ... 5 μg of total RNA from wild-type HEK293T and C6orf203-KO cells were resolved on a 1.5% agarose gel containing 18% formaldehyde and transferred to a ZETA-PROBE GT membrane (Bio-Rad). Probes used for hybridization were 32P-labeled specific antisense ssRNA corresponding to 16S-rRNA gene (or MT-RNR2) ...
-
bioRxiv - Biochemistry 2021Quote: ... and the proteins containing 5× SYPRO™ Orange were loaded onto Hard-Shell® 96-well PCR Plates (Bio-Rad) for measurement ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing 2 μl of five times diluted cDNA and monitored by CFX Connect Real-Time PCR Detection System (Bio-Rad). The following cycling parameters were used ...
-
bioRxiv - Microbiology 2020Quote: ... at 5% parasitemia was resuspended in 300 μL CytoMix containing 75 μg plasmid DNA in a 0.2-cm electroporation cuvette (Bio-Rad). Electroporation was performed at 0.31 kV and 950 μF with maximal capacitance using a Gene Pulser II system (Bio-Rad) ...
-
bioRxiv - Neuroscience 2022Quote: ... Aliquots of the lysates containing 10 mg of protein were denatured in Laemmli buffer and β-mercaptoethanol (Bio-Rad, USA) at 95°C for 5 min ...
-
bioRxiv - Developmental Biology 2022Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Nuclear and cytosolic fractions of iPSCs-differentiated cells were acquired using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific #78833 ...
-
bioRxiv - Microbiology 2020Quote: ... Protoplast suspensions (2 × 108 cells/ml) containing 0.6% low melting agarose gel were added to 50-well dispensable mold plates (Bio-Rad). Plugs were immersed in 10 ml of NDS (1% N-lauroyl sarcosinate sodium salt solution ...
-
bioRxiv - Biochemistry 2020Quote: ... 40 μL of electrocompetent cells were transformed with 1 ng of the lesion-containing plasmid mixed with 1 ng of pVP146 (internal control for transformation efficiency) by electroporation using a GenePulser Xcell (BioRad) at 2.5 kV ...
-
bioRxiv - Cell Biology 2020Quote: Nucleosome fractions containing 200 ng DNA were applied for SDS-PAGE with 4-20 % gradient SDS-PAGE gel (Bio-rad). Proteins were transferred to the nitrocellulose membrane (GE ...
-
bioRxiv - Physiology 2019Quote: ... individual cardiomyocytes were immediately aspirated and deposited into separate microcentrifuge tubes containing SingleShot Cell Lysis buffer (Bio-Rad, Hercules, CA) supplemented with DNase ...
-
bioRxiv - Genomics 2021Quote: qPCR reactions were carried out in 25 µl total volume containing 5 ng of cDNA sample and 0.3 µM designed primers and SYBR Green Master Mix (Bio-Rad). Amplification was performed by Step One Plus (Applied Bio System ...
-
bioRxiv - Biophysics 2021Quote: ... The solution was then transferred to a tube containing ∼30- 50 mg of Bio-Beads (Bio-Beads SM-2, BioRad) that were previously equilibrated in 20 mM Tris-HCl pH 7 ...
-
bioRxiv - Immunology 2020Quote: ... and membrane blocking was performed overnight at 4°C in Tris-buffered saline (TBS) containing 0.2% Tween 20 (V/V) (TBST) and 5% (W/V) Blotting-Grade Blocker (Bio-Rad). Following overnight blocking ...
-
bioRxiv - Genetics 2019Quote: ... All qPCR reactions were performed in a total volume of 5 µl containing 2.5 µl of SsoAdvanced mastermix (Bio-Rad), 2 µl of forward and reverse primers (5 µM ...
-
bioRxiv - Cell Biology 2021Quote: ... PCR amplicons were resolved in 1% agarose (Melford) TBE gels containing 1x SYBRsafe and visualised on a Chemidoc MP (BioRad). A full list of oligonucleotides and vectors are presented in Table S1 ...
-
Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
-
bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated 3 h with a solution containing a secondary goat-HRP anti rabbit (1:10 000) (Biorad) (milk 0.5 % TBST) ...
-
bioRxiv - Microbiology 2022Quote: ... Approximately 67 ng of target oligonucleotides (Integrated DNA Technologies) were used with and without 100 ng of pKM496 for electroporations using cuvettes containing 0.2 cm gaps (Bio-Rad). Following electroporation ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20-100 µg of sheared chromatin used as input and ChIP beads were then boiled 10 min in 100 µL H2O containing 10% (V/W) chelex resin (BioRad), followed by Proteinase K (0.2 mg/mL)-digestion for 30 min at 55°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... PVDF membranes were blocked for 1 hr in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4°C with antibodies listed in Table S1 in 5% BSA in TBS-T ...