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Citations for Bio-Rad :
6551 - 6600 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Clear supernatants were transferred to a clean tube and quantified using DC Protein Assay (5000112, BioRad). Alternatively ...
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bioRxiv - Biochemistry 2023Quote: ... the concentrations of the supernatant were measured with an RC DC protein assay kit (Bio-Rad). Supernatant concentration was adjusted to the lowest concentration and lysis buffer was added to bring all volumes to 1mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE (10% Mini-PROTEAN® TGX™ Precast Gel, Biorad, 4561036) and transferred to a PVDF membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA). These blots were blocked with 4% skimmed milk in 1X TBST (for anti-phospho threonine antibody 4% BSA in 1X TBST ...
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bioRxiv - Genetics 2023Quote: ... The imaging and quantification of target proteins was obtained by ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Genetics 2023Quote: ... Protein lysate was subjected to SDS-PAGE and subsequently electrotransferred to a PVDF membrane (Bio-rad). Then primary antibodies and HRP conjugated secondary antibodies were incubated with the 5% milk blocked membrane ...
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bioRxiv - Neuroscience 2023Quote: ... Samples with equal amounts of proteins were added to 4× Laemmli sample buffer (1610747, Bio-Rad) supplemented with DTT (10197777001 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to nitrocellulose membranes using the semi-dry Trans-Blot Turbo Transfer System (Biorad) for 10 min at constant 20 V (2.5 limit A) ...
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bioRxiv - Developmental Biology 2023Quote: ... After electrophoresis and protein transfer onto 0.2μm nitrocellulose membranes (Bio-Rad Laboratories Inc., Hercules, CA, USA), membranes were blocked in fetal bovine serum or skim milk and incubated with primary antibodies (Supplementary Table 4 ...
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bioRxiv - Developmental Biology 2023Quote: ... 30 μg protein was separated on a 10% SDS-PAGE gel (TGX Stain-Free FastCast, BioRad) and transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Neuroscience 2023Quote: ... Protein bands were detected by chemiluminescence using ECL Western blot detection reagents (Bio-Rad, #170-5060).
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bioRxiv - Cancer Biology 2023Quote: ... Samples (20µg) were resolved on 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) in running buffer (25mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... Protein bands were transferred to the PVDF membrane using the Trans-Blot Turbo system (Bio-Rad). Membranes were blocked in 5% dry milk in TBST for 60 min at room temperature and incubated with diluted primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were probed with either secondary antibodies goat anti-mouse-HRP (Bio-Rad, 1706516, 1:5000) or goat anti-rabbit-HRP (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μg protein samples were heated at 95 °C in 4x Laemmli sample buffer (Bio-Rad) for 5 min Samples were run down a Novex 4–12% Tris-glycine gel (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... 20-50 µg of membrane protein fraction (concentration determined by Bradford 1X Dye reagent (Bio-Rad)) in a total volume of 15-30 uL 1x gel loading sample buffer (250 mM Tris HCl pH 6.8 ...
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bioRxiv - Cancer Biology 2023Quote: Whole-cell lysates were extracted with RIPA buffer and quantified by DC Protein Assay (Biorad 5000111). Protein samples were separated by SDS-PAGE gels (Biorad 4561086 ...
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bioRxiv - Cancer Biology 2023Quote: ... and separated by SDS-PAGE using 10% Criterion TGX Precast Midi Protein Gels (BioRad, #567-1035), Tris-Glycine-SDS running buffer (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... 20–50µg of protein lysate was subsequently transferred to a 0.22µM nitrocellulose membrane (Bio-Rad Laboratories). Primary antibodies (Supplementary Table 3 ...
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bioRxiv - Plant Biology 2023Quote: ... Three µg of protein was run on a 4-20% gradient SDS-PAGE gel (Bio-RAD), and protein bands were visualized by Coomassie Blue staining.
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bioRxiv - Plant Biology 2023Quote: ... Total protein samples were separated on a 4-20% Tris-glycine stain-free polyacrylamide gel (BioRad) at 180 V for 1 hour in 1x Tris/glycine/SDS running buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer onto polyvinylidene difluoride membrane was performed using a Trans-blot turbo system (Bio-Rad). After blocking with 5% milk ...
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bioRxiv - Microbiology 2023Quote: ... The final protein concentration was determined with a Bradford assay (Bio-Rad Laboratories, Inc, Hecules, CA) and is reported as molar dimer ...
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bioRxiv - Cell Biology 2023Quote: Proteins were resolved by SDS-PAGE and transferred to 0.2 μm nitrocellulose membranes (Bio-Rad, #1620112). The membranes were blocked overnight at room temperature with a blocking buffer (Li-Cor Intercept Blocking Buffer TBS ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins in supernatant were analyzed by gel electrophoresis on 4%–20% gradient polyacrylamide gels (BioRad, 4561093) and transferred onto nitrocellulose membrane (BioRad 1704158) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein transfer to nitrocellulose for blotting was performed using the Trans-Blot Turbo Transfer system (BioRad), with a standard or high-molecular weight protocol as appropriate ...
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bioRxiv - Microbiology 2023Quote: ... proteins separated by SDS-PAGE were transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad, cat#1620112). After blocking with PBST buffer containing 5% non-fat milk at RT for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated in 4–15% Mini-PROTEAN® TGX Stain-Free™ acrylamide gels (BioRad) and transferred onto 0.45 μm low-fluorescence PVDF membranes (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... Elution fractions were analyzed on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and fractions containing the gp120 glycoprotein were concentrated followed by buffer exchange to PBS using Vivaspin 6 centrifugal concentrators (30kDa ...
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bioRxiv - Immunology 2023Quote: ... and then loaded onto protein gels (4-20% Mini-PROTEAN® TGXTM Precast Gels, Bio-Rad). After gel electrophoresis ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved on 4–20% gradient precast Mini-PROTEAN TGX polyacrylamide gels (BIO-RAD #4561094) and transferred to a 0.45 mm nitrocellulose blotting membrane (Amersham™ #10600002 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to PVDF/nitrocellulose membranes using the Transblot semi-dry blotting system (Bio-Rad). Transfer was usually perfomed at 25 V for 7 min ...
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bioRxiv - Cell Biology 2023Quote: ... Approximate molecular masses were determined by comparison with the migration of prestained protein standards (Bio-Rad). Uncropped gel images are presented in Supplementary Fig ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of protein were loaded onto precast 4-15% gradient SDS-PAGE gels (Bio-Rad) and run at 100V for between 1 – 1 ½ hours ...
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bioRxiv - Biochemistry 2023Quote: ... Protein from a 12.5% polyacrylamide gel were transferred onto an Immuno-blot PVDF membrane (Bio-rad). The membranes were stained with 1% Ponceau and imaged using the ImageQuant LAS 500 ...
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bioRxiv - Developmental Biology 2023Quote: Cell fractionation was performed as described (35) and quantified using the DC protein assay (Bio-Rad). Nuclear extracts were separated by electrophoresis on 10% SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Biophysics 2023Quote: All proteins and pseudovirions were run on 4-20% denaturing polyacrylamide gels (Bio-Rad, Hercules, CA) and transferred to nitrocellulose membrane using Trans-blot Turbo (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... Protein samples were transferred to blotting paper using a Trans-Blot Turbo Transfer Pack (Bio-Rad) and a Trans-Blot Turbo (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... 50 µg protein was loaded in each well of precast 4-15% Bis Tris gels (BioRad) and run for 2 hours at 80V ...
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bioRxiv - Cell Biology 2024Quote: ... The protein samples were separated by SDS-PAGE on a 4–20% gradient gel (Bio-Rad) and transferred to a 0.45-µm nitrocellulose membrane (Pall Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated via SDS-PAGE using precast 4-20% gradient gels (Bio-Rad, Criterion TGX) and blotted onto nitrocellulose membranes (supernatants ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μg protein samples were heated at 95 °C in 4x Laemmli sample buffer (Bio-Rad) for 5 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... Equal amounts of whole cell protein were separated on a 15% SDS-polyacrylamide gel (Bio-Rad) by SDS-PAGE ...
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bioRxiv - Biochemistry 2024Quote: Protein samples were run on 4-20% or 7.5% Mini-PROTEAN TGX SDS-PAGE gels (BioRad), then transferred to PVDF membranes using the TurboBlot semi-dry transfer system (BioRad) ...
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bioRxiv - Cell Biology 2024Quote: ... The ladder and proteins in the gel were transferred to the PVDF membrane (Bio-Rad 1704156) by the semi-dry method ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred to Nitrocellulose membranes using the semi-dry Trans-Blot Turbo system (Bio-Rad). Membranes were then blocked in 5% milk/PBS-T (PBS containing 0.1% Tween 20) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane using a Trans-Blot Turbo (Bio-Rad) semi-dry blotting system according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 12.5μg of total protein per sample were then loaded onto 12% Criterion TGX gels (BioRad, #5671044) and run at 65 V ...
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bioRxiv - Immunology 2024Quote: ... the samples were rotated with 100 µL SureBeads™ Protein G Magnetic Beads (#1614023, Bio-Rad) for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and were run on a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad). Proteins were transferred on a nitrocellulose membrane and blocked for two hours at room temperature in blocking solution and incubated in primary antibody solution for 48 hours at 4°C ...