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Citations for Bio-Rad :
6401 - 6450 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 25μg of protein were denatured at 95°C for 5min in Laemmli Sample Buffer (BIORAD #1610747) with DTT 0.1M ...
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bioRxiv - Physiology 2022Quote: ... 10-20 µg protein samples in SDS-PAGE were resolved in 7.5% -10% gels (Bio-Rad) and transblotted onto polyvinylidene fluoride membranes (Millipore) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to a PVDF membrane using the Trans-Blot Turbo transfer system (Bio-Rad). Blots were probed with primary antibodies and donkey anti-mouse or goat anti-rabbit secondary antibodies conjugated to IRDye® 800CW or 680RD flurophores (LI-COR) ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was transferred to a Trans-Blot Turbo Mini 0.2 µm Nitrocellulose membrane (BioRad, 1704158) using the Trans-Blot Turbo Transfer System from BioRad ...
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bioRxiv - Immunology 2022Quote: ... the proteins were incubated 5 minutes at 95°C in Laemmli sample buffer (Bio-Rad laboratories), with or without addition of β-mercaptoethanol (Sigma-Aldrich Chemie) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Protein samples were transferred to blotting paper using a Trans-Blot Turbo Transfer Pack (Bio-Rad) and a Trans-Blot Turbo (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using Enhanced-Luminol-based chemiluminescent substrate (ECL; Catalog no. 1705060; Bio-Rad, India) and visualized using an I-Bright CL1000 Chemidoc system (Thermofisher) ...
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bioRxiv - Microbiology 2022Quote: ... The protein sample was diluted with Laemmli buffer containing β-mercaptoethanol (Bio-rad, Hercules, CA, USA) and incubated at 65°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Protein bands were visualized by staining with Coomassie Brilliant Blue G-250 (161–0406, Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... The proteins were transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and the total amount of protein was determined via Bradford assay (BioRad). 30 µg of protein was added and verified using a mouse anti-BetaActin mAb (CellSignaling) ...
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bioRxiv - Developmental Biology 2022Quote: ... Equal amounts of proteins were loaded onto a 4%-20% SDS-PAGE gel (BIO-RAD, #4561094) and run at 130V and 30A for 1.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... a gel was cut into 11 slices based on protein molecular weight ladder (Biorad, Cat # 1610394) (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... then lysates were loaded on 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Separated proteins were transferred onto a PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration in each lysate sample was quantified with Quick Start Bradford 1× Dye (BioRad) to equalise protein concentrations across the samples before SDS-PAGE and immunoblotting.
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bioRxiv - Physiology 2023Quote: ... Protein separation occurred by SDS-PAGE using a mini– PROTEAN Tetra electrophoresis system (Bio-Rad Laboratories) at 100V for 2h and held at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Protein separation occurred by SDS-PAGE using a mini PROTEAN Tetra electrophoresis system (Bio-Rad Laboratories). The separated proteins were then transferred to nitrocellulose membranes using the same apparatus and membranes were then blocked in 3% nonfat dry milk rehydrated in Tris-Buffered saline containing 1% Tween (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Western blots were performed using AnykD Criterion TGX Precast Midi Protein Gels (Bio-RAD, 5671124, 5671123). Gels were run in 1x Tris-Glycine SDS Running Buffer (Thermofisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Protein concentrations of post 800 x g lysates were estimated by Bradford DC Assay (BioRad Laboratories) then 60 μg protein/lane was loaded into a 4-12% Bis-Tris gel ...
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bioRxiv - Synthetic Biology 2023Quote: ... and then run on an Any kD Mini-PROTEAN TGX Stain-Free Protein gel (Biorad, 4568124). SDS-PAGEs were captured on the Biorad Chemidoc Imaging System ...
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bioRxiv - Plant Biology 2022Quote: ... The protein concentration was determined with the Bradford method (Quick Start™ Bradford Reagent, Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... and 20ug protein was loaded on a 4%-12% acrylamide gel (Criterion TGX,Cat.#5671095, Biorad). The samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein lysate was run on a 4–20% SDS PAGE and transferred to nitrocellulose membrane (BioRad). Blots were blocked with 5% skim milk in TBS-T (Tris-buffered saline with 0.1% Tween 20 ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were separated by SDS–PAGE and transferred to a nitrocellulose membrane (0.2 µm, Bio-rad) by the wet-tank transfer method and blocked with 5% non-fat milk in TBST for 1 h at room temperature before the overnight inoculation in primary antibody at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2022Quote: ... protein samples were separated on SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad, 88585) using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred to a nitrocellulose membrane using the iBlot 2 Dry Blotting System (Bio-Rad) with pre-assembled dry transfer stacks ...
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bioRxiv - Neuroscience 2022Quote: ... 3 final 10-minute TBST washes were followed by visualization of proteins with ECL (BioRad, 11705062). Densitometry analysis was performed using ImageLab 6.0 software (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the cell extracts was determined using the BCA assay kit (Bio-Rad). Protein concentration for lysates was adjusted to 2 μg/μl before adding 4X loading buffer (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... protein lysates were prepared for western blotting by diluting samples in 4x Laemmli Buffer (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... Protein was eluted from the beads by adding Laemmli sample buffer (Bio-rad, Laboratories, Inc., USA), Sample Reducing Agent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... pastoris transformants were screened for protein expression by immuno-colony blot using nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... This protein-lipid-DDM suspension was then incubated with Bio-Beads SM-2 resin (Bio-Rad) to gradually remove the detergent (8 ...
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bioRxiv - Neuroscience 2022Quote: ... The PBS was removed and replaced with 30μL of 4x Laemmli protein buffer (Bio-Rad #1610747) supplemented with β- mercaptoethanol ...
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bioRxiv - Plant Biology 2024Quote: ... proteins were separated by SDS–PAGE and transferred to a nitrocellulose membrane (0.2 µm, Bio-rad) by the wet-tank transfer method and blocked with 5% non-fat milk in TBST for 1 h at room temperature prior to the overnight inoculation in primary antibody at 4 °C with gentle rotation ...
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bioRxiv - Neuroscience 2023Quote: ... Electrophoresed proteins were transferred onto a 0.2 µm nitrocellulose membrane (Bio-Rad, Hercules, CA, United States) using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to PVDF membranes using the Trans-Blot® Turbo™ Transfer System (BioRad) and membranes were blocked with 5% skimmed milk in TBST buffer for 1 h and incubated overnight at 4 °C in TBST with the following primary antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... proteins separated on 10% SDS-PAGE gels and transferred to PVDF membrane (BIO-RAD, catalog #1620177) for immunoblotting ...
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bioRxiv - Pathology 2024Quote: ... the proteins were transferred via the TransBlot Turbo semi-dry blotting apparatus (Bio-Rad Laboratories, #1704150) onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: Total protein extract (10 µg) was separated by SDS-PAGE and transferred to PVDF membranes (BioRad). For detection of biotinylated proteins as result of TurboID activity ...
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bioRxiv - Cell Biology 2024Quote: ... Separated proteins were transferred to 0.2µm and 0.45µm nitrocellulose membranes (Biorad Cat. no. 1620115 and 1620112) for transferring proteins below and above 180kDa ...
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bioRxiv - Neuroscience 2024Quote: ... Results were normalized to total protein content visualized by a TGX stain-free method (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Protein was separated by SDS-PAGE and transferred to PVDF membrane using the TransBlot Turbo (BioRad). Membranes were blocked with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Neuroscience 2024Quote: ... 30ug total protein was denatured under reducing conditions in sample loading buffer (Bio-Rad, Hercules, CA) by boiling for 10 min at 98°C before loading onto a 4-20% gel (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... Proteins were transferred to nitrocellulose membranes using Trans-Blot® Turbo™ Blotting System (Bio-Rad). Membranes were blocked with 5% skimmed milk (Scientific Laboratory Supplies ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo (BioRad Laboratories). Nonspecific binding to the membrane was blocked by incubating in Odyssey Blocking Buffer for 60 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... A wet transfer of the proteins onto a 0.2 μm polyvinylidene fluoride (PVDF) membrane (Biorad, 1620177) was then carried out ...
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bioRxiv - Molecular Biology 2024Quote: ... and the total protein concentration was measured using the Bradford method (Bio-Rad, Hercules, CA, USA). For WB ...
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bioRxiv - Microbiology 2024Quote: 10 μg of each protein sample was loaded into 4-20% TGX Gradient Gels (Bio-Rad) for analysis ...
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bioRxiv - Cell Biology 2024Quote: ... and 20-50 μg of protein was run on 4-20% gradient pre-cast Gels (Biorad) and transferred using Trans-Blot (Biorad ...