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Citations for Bio-Rad :
6351 - 6400 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cDNA was prepared using iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA (500ng) was reverse transcribed to single stranded cDNA using the iScript cDNA Synthesis Kit (BioRad). Real-time PCR reactions using ssoFast PCR mastermix (Biorad ...
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bioRxiv - Neuroscience 2023Quote: ... DNAse-treated RNA samples were reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad, 1708891), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... and used directly for quantitative PCR (qPCR) analysis with the SYBR green qPCR kit (Bio-Rad). Signals obtained by qPCR were normalized to 18S unless otherwise noted ...
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bioRxiv - Developmental Biology 2023Quote: First-strand cDNA synthesis was carried out using the Iscript cDNA synthesis kit (Biorad, Hercules, USA) following the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... both the cytoplasmic and WC fractions were reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad) and 12S mitochondrial rRNA primer was used for both the cytoplasmic and WC fractions (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2023Quote: ... reverse transcription was performed with the iScript gDNA Clear cDNA Synthesis Kit (BIO-RAD, Cat. # 1725035) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were determined using a Micro Bradford Protein Assay Kit (Bio-Rad, Rockford, IL, USA). Samples with equal quantities of protein (50 μg ...
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bioRxiv - Immunology 2023Quote: ... Then gene expression was determined by SYBR green real-time PCR using SYBRTM Premix kit (BioRad) and was expressed using the 2 -ΔΔCt method ...
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bioRxiv - Bioengineering 2024Quote: ... The protein amount of the cleared lysates was measured using the DC protein assay kit (BioRad) according to the manufacturers protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 to 1000 ng was reverse transcribed using the iScript cDNA Synthesis kit (Bio-Rad, 1708891) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Reducing SDS-PAGE gel was made using the SDS-PAGE Gel kit (Biorad, Cat. No. 1610183). 15 μg sample was loaded into SDS-PAGE gel and transferred onto PVDF membranes (Biorad ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of the supernatant was determined using a Bradford protein assay kit (Bio-Rad). The brain lysates were then denatured in SDS sample buffer and separated by SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... 900 ng of RNA was reverse transcribed using the iScript cDNA Synthesis Kit (#1708891, Bio-Rad). SensiFAST SYBR Hi-ROX kit (Bioline ...
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bioRxiv - Biophysics 2024Quote: ... the isolated RNA was converted to cDNA using an iScript cDNA Synthesis Kit (Bio-Rad, USA). The polymerase chain reaction (PCR ...
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bioRxiv - Bioengineering 2024Quote: ... cDNA was generated from the isolated RNA using an iScript™ cDNA Synthesis Kit (Bio-Rad), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis was performed with 1μg of total RNA using an iScript Reverse Transcription Kit (Biorad). qPCR reactions were made with the 2x SYBR Green Master Mix (Biorad) ...
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bioRxiv - Microbiology 2024Quote: ... Retrotranscription was performed thanks to the iScript cDNA synthesis kit following the manufacturer’s instructions (Bio-Rad). Quantitative RT-PCRs (qRT-PCRs ...
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bioRxiv - Neuroscience 2024Quote: ... qRT-PCR was performed using the EvaGreen kit (Axon) with a CFX96 Real Time System (BioRad). The primer sequences used are listed in Supplementary Table 1.
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bioRxiv - Neuroscience 2024Quote: ... The upper phase was collected and RNA extracted using the Aurum total RNA kit (Bio-Rad) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using the Bradford assay (Bio-Rad Protein Assay kit, Bio-Rad, USA). Equal amounts of protein from each sample were dissolved in sample buffer [Laemmli with 5% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using the Bradford assay (Bio-Rad Protein Assay kit, Bio-Rad, USA). Equal amounts of protein from each sample were dissolved in sample buffer [Laemmli with 5% (v/v ...
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bioRxiv - Genomics 2024Quote: ... cDNA synthesis was performed with the iScript Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Droplets were generated and quantitated on the QX200 Droplet Digital PCR System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... The bead-captured EU-RNAs were converted to cDNA using iScript DNA synthesis kit (Bio-Rad) and further subjected to real-time PCR analysis.
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bioRxiv - Microbiology 2024Quote: ... Complementary (c) DNA was synthesized by using a qScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The sequence of the primer sets for CHIKV capsid protein ...
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bioRxiv - Immunology 2022Quote: ... diluted to 1:5,000 in 1X TBST 5% milk powder for 1 hour before visualisation with Clarity™ Western ECL Substrate (Bio-Rad, Hercules, California, US) using a Syngene G ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies (Supplemental Table 1) were incubated for 1 hour at RT and protein bands were visualized using a ChemiDoc XRS+ (Bio-Rad Laboratories, Hercules, USA).
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bioRxiv - Cell Biology 2022Quote: ... Protein lysate (30 μg) with 2X Laemmli buffer (in 1:1 ratio) was loaded onto a 4%–20% gradient gel (Bio-Rad, Mississauga, ON, Canada). The gel was transferred onto a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... PE-conjugated anti-Foxp3 mAb (clone FJK-16s, eBioscience™, 1:20) and/or Alexa Fluor 647-conjugated anti CD3 mAb (clone CD3-12, Bio-Rad, 1:100) were added directly to cells and incubated for 40 min ...
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bioRxiv - Molecular Biology 2024Quote: ... A 1:1 cDNA dilution was done to perform real-time PCR using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad cat no#172-5271) in CFX connect Real-Time instrument (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-solubilized proteins (0.2-0.3 OD600 cell equivalents) were resolved by 10% SDS-PAGE prior to transfer to nitrocellulose membranes using the Trans-Blot Turbo™ system (Bio-Rad Laboratories, Hercules, CA) and blocked with Western Blocking Reagent (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Each 20 μL qPCR reaction was prepared in UV-treated UltraPure nuclease-free water containing 10 μL of EvaGreen® Supermix (Bio-Rad Laboratories, Hercules, CA), 0.5 μL of each primer (forward and reverse ...
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bioRxiv - Neuroscience 2019Quote: ... Reactions were carried out in triplicate on 96-well plates with 10 µL reactions per well using a CFX96 Touch™ Real-Time PCR Detection System from Bio-Rad (Hercules, CA, USA). Cycling conditions for qPCR reactions were as follows ...
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bioRxiv - Microbiology 2020Quote: ... Amount 40 µg of total protein was run in 10% polyacrylamide gel at the same electrophoresis condition and then the gel was stained in coomassie blue solution (Bio-Rad Laboratories, Berkeley, USA; #1610786).
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bioRxiv - Genetics 2022Quote: ... Real time (RT)-qPCR was carried out in a Bio-Rad® CFX-96™ thermocycler in 10 μl final volume reaction using SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and 50 pmol of each forward and reverse primer (Table S1) ...
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bioRxiv - Neuroscience 2021Quote: ... and half of each biotinylated fraction were separated into 10% SDS-PAGE gels electrophoresis and transferred to nitrocellulose membranes (Bio-Rad Laboratory, Hercules, CA, USA). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... pelleted by centrifugation and resuspended in RPMI medium containing 10% foetal bovine serum where cell count and viability was estimated (BioRad, TC20™ Automated Cell Counter) before plating for experiments.
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of proteins from lysates were separated by SDS-PAGE using Mini-PROTEAN® TGX™ 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... and heated to 95°C for 10 minutes before being loaded into precast gels (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad, Cat. # 4561094, 4561096). The Precision Plus Protein™ Dual Color Standards (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were transferred to new tubes containing 87% glycerol (final concentration of 10%) and concentrations were adjusted using Bio-Rad protein assay (Bio-Rad, Hercules, California, United States).
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bioRxiv - Biochemistry 2023Quote: ... The resin was recovered and washed twice with 10 column volumes of S100 Lysis Buffer on a gravity flow column (Bio-Rad Laboratories Inc., Hercules, CA). The S100 extract was eluted from DEAE resin with 5 ml of S100 Elution Buffer (250 mM KHPO4 (pH 6.5),1x Mini protease inhibitor cocktail tablet (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed three times for 10 mins with TTBS and once for 10 mins with TBS before developing it with the Clarity Western ECL Substrate (Bio-Rad Laboratories, cat. #170-5061) and visualizing it using the Bio-Rad ChemiDoc MP Imaging system ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μg of total proteins were resolved on 10% polyacrylamide gels (except for 6% gels used for PERK detection) and blotted onto PVDF membranes (#1620177, Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked with 5% not-fat dry milk or bovine serum albumin (#A7906 ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 μg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... The same amount of cell lysates or immune precipitates was resolved by 10% SDS-PAGE and transferred to the PVDF membrane (Bio-Rad Laboratories, Hercules, CA, USA) by electrophoresis ...
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bioRxiv - Biochemistry 2023Quote: ... BioRad Bradford Assay was carried out and 10 µg of each sample was applied in SDS-PAGE gels and then transferred to a PVDF membrane (Bio-Rad, 0.45 µm pore size) by electro-transfer (30 V constant overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Bioengineering 2023Quote: ... The qPCR reactions were performed in a total volume of 20 µL with 10 µL of iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories Inc.), 0.7 µL of the forward primer (350 nM) ...
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bioRxiv - Neuroscience 2024Quote: ... was boiled for 10 min at 97°C and loaded into 8% SDS-PAGE gels with Precision Plus Protein Standard (Bio-Rad, 161-0374/161-0375). Separated proteins were transferred to a nitrocellulose membrane (0.2 μm ...
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bioRxiv - Molecular Biology 2024Quote: ... were resolved on a 4-15% or 10% acrylamide gel and transferred onto a PVDF membrane (Invitrogen iBlot 2 or Bio-Rad Trans-Blot Turbo system). Membranes were blocked with 5% milk (standard protein detection ...