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Citations for Bio-Rad :
6251 - 6300 of 7138 citations for 7 Chloro 4 hydroxy 1 8 naphthyridine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 ug) was converted to cDNA using the iScriptTM cDNA synthesis kit (# 1708891, BIO-RAD, Hercules, CA) following the manufacturer’s protocol (BIO-RAD ...
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bioRxiv - Bioengineering 2021Quote: ... Amplifications were performed in a 20 μL reaction containing 1 × ddPCR Supermix for Probes (No dUTP),(Bio-Rad, 1863024), 250 nM of probes and 900 nM of primers and 8 μL EV DNA template ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were developed with a peroxidase-labeled goat anti-mouse or anti-rabbit secondary antibody (1:5,000, Biorad) and chemiluminiscence detection (MF-ChemiBIS 3.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates prepared at a concentration of 1 mg/ml and supplemented with 4x Laemmli Sample Buffer (Bio-Rad: 1610747) supplemented with fresh 2-mercaptoethanol (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and exposed to X-ray film (Kodak) or the ChemiDoc Imaging system (Biorad) after incubation with Thermo Scientific Pierce ECL or Clarity ECL (Biorad).
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bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit or anti-mouse IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Biochemistry 2021Quote: Reaction products were analysed by 20% denaturing polyacrylamide gel electrophoresis (made from 40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... samples were combined and run on a 20% denaturing polyacrylamide gel (40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-syntenin-1 (SCBT, sc-515538) and horseradish peroxidase-conjugated secondary goat anti-mouse (Bio-Rad, 170-6516), goat anti-rabbit (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... at a 1:10,000 dilution and we developed blots using the AP conjugate substrate kit (Bio-Rad; Hercules, USA). We used polyclonal GFP antibodies (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
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bioRxiv - Plant Biology 2021Quote: ... Four- to six-week-old expanded leaves of relevant Arabidopsis lines were bombarded with 1 nm gold particles (BioRad) coated with pB7WG2.0-mRFP ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were detected using anti-mouse IgG horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3,000; catalog number NEF822001EA, Perkin-Elmer) and developed with Clarity Western ECL Substrates (BioRad). All antibodies were diluted in TBS-T with 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... Chromosomes were separated on a 1% pulse-field certified agarose gel on the CHEF Mapper XA system (Bio-Rad) under the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... cDNA samples were diluted 1:15 and used for qPCR with the iTaq Universal SYBR green mastermix (Biorad, #1725120). Cycle threshold values were corrected for PCR efficiency of each primer set and normalized to the hypoxanthine phosphoribosyltransferase 1 (HPRT ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit monoclonal antibody specific for Aβ42 (1:2,000, BioLegend 812101. Secondary antibodies were fluorescently labelled. Fluorescence was detected on a ChemiDoc MP system (Biorad). Immunohistochemistry was carried out as described (44) ...
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bioRxiv - Cell Biology 2021Quote: ... This was followed by incubation with a secondary HRP-conjugated anti-rabbit or anti-mouse antibody (1:2000, BioRad), depending on the origin of the primary antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... We used 1 μg of RNA to produce cDNA with iScript™ Reverse Transcription Supermix (Bio-Rad, Cat#1725120). The cDNA was subjected to quantitative real-time PCR with iTaq™ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2022Quote: ... small RNAs were isolated (18nt–30nt) from 1 μg of total RNA using 15% urea-PAGE (Bio-Rad, USA) followed by overnight precipitation using 0.3% NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradient was fractionated using a Gradient Station (BioComp) coupled with an ultraviolet 254nm detector (Bio-Rad EM-1).
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bioRxiv - Microbiology 2022Quote: ... the samples were washed extensively with lysis buffer and the proteins were eluted by boiling 5 ul of the resin with 5x SDS loading buffer and the proteins were detected by western blot analysis using rabbit antibody to FLAG (1:2,000, BioRad) or mouse antibody to Myc (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL protein was injected onto either a self-packed buffer exchange column (P6 polyacrylamide gel, Bio-Rad Laboratories) or a prototype desalting column from Thermo Fisher Scientific and online buffer exchanged to 200 mM ammonium acetate ...
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bioRxiv - Molecular Biology 2022Quote: ... were used at a concentration of 1:2,000 and signals were visualised using Clarity Western ECL substrate (Bio-Rad) on a ChemiDoc MP (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of each RNA sample was reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR was performed on cDNA samples using the iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... the tubes were dialyzed twice for 48-72h against buffer I (25mM Tris pH 7.4, 25 mM NaCl, 1 mM β-mercaptoethanol and 0.5 g of Bio-Beads (Biorad). After dialysis ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of each cDNA sample was amplified with gene-specific primers in iQ SYBR Green Supermix (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed two times with TBST and then incubated with a 1:5,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed with TBST and then incubated with a 1:10,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... Membranes were washed and probed with secondary antibody (1:5000, anti-Rabbit-HRP, 1706515; anti-Mouse-HRP 1706516, BioRad) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... The master mix for droplet digital PCR (ddPCR) amplification included 1× ddPCR Supermix for probes (no dUTP, Bio-Rad), 0.2 1M of each primer and 0.2 1M probe (both IDT ...
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bioRxiv - Microbiology 2022Quote: ... we used 10µL of desalted assembly reaction products to transform competent E.coli S17-1 cells (mid-log cells, washed three times in deionized ice-cold water) by electroporation (V=1.8kV; MicroPulser, BioRad). After 1h recovery at 37°C in 1mL of LB ...
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bioRxiv - Pathology 2022Quote: ... The expression levels of target proteins were quantified with Quantity One 1-D Analysis Software (Bio-Rad, Hercules, CA) and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Zoology 2019Quote: ... and specific primers (Table 1) on a CFX96 Touch™ Real-Time PCR Detection System (Biorad, Hercules, CA, USA). Actin was used as housekeeping gene normalization control (Table 1) ...
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bioRxiv - Microbiology 2019Quote: ... Blots were incubated with appropriate horseradish peroxidase -conjugated secondary antibody goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... Cells were then stained for inflammasome activation using a caspase-1 FAM-YVAD-FMK FLICA probe kit (Bio-Rad). Cells were then processed for analysis by flow cytometry.
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription was performed on 1 μg of purified RNA using the iScript Reverse Transcription Supermix kit (Bio-Rad). The cDNA was then added to SsoFast (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then pelleted in a 1.5 mL tube and resuspended in 1 mL phosphate buffered saline pH 7.8 (Bio-Rad) (PBS) ...
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bioRxiv - Cell Biology 2019Quote: ... Target transcripts were amplified using primers listed in Table 1 and Sso Advanced Universal SYBR Green Supermix according to manufacturer’s protocol (BioRad). All qPCR reactions were assayed in triplicate for each sample and the average Cq value was used to calculate the mean expression ratio of the test sample compared to the control sample (i.e ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription of 1 μg RNA/20μl was performed with the iScript™ cDNA Synthesis Kit (1708890, Bio-Rad). qPCR was performed with iTaq™ Universal SYBR® Green Supermix (1725120 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed 3 times with PBS and then blocked for 1 hour at room temperature with DPBS with 0.4% Triton X-100 and 5% non-fat dry milk (BioRad). Cells were stained with primary and secondary antibodies (Table S3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with respective anti-mouse or anti-rabbit HRP-conjugated secondary antibody (1:1000, Bio-Rad) and visualized with a chemoluminescence kit (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: ... which was inoculated at 30°C for 1 hour and then photographed using a UV transilluminator (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... Amplification products were resolved in 1% (w/v) agarose gel and analysed with GelDoc XR+ System (Bio-Rad Laboratories). Primers and probes are listed in Table S1.
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were detected with anti-mouse HRP-conjugated secondary antibodies (1:5000; Bio-Rad, Hercules, CA, USA), and the signal was developed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used: rabbit anti-mouse collagen-IV (Bio-Rad Company, cat.# 2150-1470, 1:300), rat anti-mouse CD45 (BD Pharmingen ...
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bioRxiv - Genomics 2019Quote: ... All blots were visualized using a goat anti-rabbit IgG:alkaline phosphatase fusion protein at 1:3,000 dilution in conjunction with BCIP/NBT color development (Bio-Rad).
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bioRxiv - Neuroscience 2019Quote: ... First-strand cDNA synthesis was performed from 1 μg isolated RNA template using iScript RT supermix (Bio-Rad #1708841). PCR products were amplified using a CFX96 Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Biophysics 2019Quote: ... Gel precursor solutions were prepared with 6% v/v acrylamide and 0.2% v/v bisacrylamide (30% acrylamide/bisacrylamide stock 29:1, BioRad), 2 mM LAP photoinitiator (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... RNA (1 μg) was reverse transcribed in 20 μl reactions using the BioRad iScript cDNA Synthesis Kit (BioRad Laboratories) with 1:1 mixture of oligo-dT and random hexamers for 30 min at 42°C ...