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Citations for Bio-Rad :
6501 - 6550 of 7138 citations for 7 Chloro 4 hydroxy 1 8 naphthyridine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Bradford Protein Assay Reagents 1 and 2 and Precision Plus Protein All Blue Prestained Protein Standard were from Bio-Rad. The anti His-tag mouse antibody was from GenScript (cat no ...
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bioRxiv - Molecular Biology 2021Quote: ... An aliquot was removed and diluted 1:10 in 1xPBS for cell counting using an automatic counter (Biorad TC-20) with counts spot checked by manual recount using a hemocytometer as needed ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplicons were resolved in 1% agarose (Melford) TBE gels containing 1x SYBRsafe and visualised on a Chemidoc MP (BioRad). A full list of oligonucleotides and vectors are presented in Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples for qPCR were prepared with primers against target genes (Table 1) and mixed with SSO Advanced Universal SYBR Green Supermix (BioRad) in 96-well plates and analyzed on a CFX96 qPCR machine (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were diluted 1:10 and 1µl of diluted cDNA was used per qPCR reaction with iTaq Universal SYBR Green supermix (Bio-Rad) and the following pairs of primers ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized using 1 µg of total RNA and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). Synthesized cDNAs were then quantified by real time quantitative PCR (qPCR ...
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bioRxiv - Immunology 2022Quote: ... 2 μm paraffin-embedded kidney sections were incubated with an anti-F4/80 antibody (CI:A3-1; Bio-Rad, Feldkirchen, Germany). After incubation with biotinylated goat anti-rat IgG (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... or ~7.5 million cells/mL (Fig S18c) before loading into wells of preformed phantoms consisting of 1% agarose (Bio-Rad) in PBS.
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bioRxiv - Neuroscience 2022Quote: 1 μg of total RNA purified from differentiated cells was reversed transcribed using the One-step SuperScript kit (Bio-Rad). The qRT-PCR reaction was performed in a CFX96 Real-time PCR detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... The following primary antibodies were used for western-blotting at 1/1,000 dilution: anti-V5 clone SV5-Pk1 (mouse, monoclonal, BioRad, #MCA1360), anti-Rpb3 clone 1Y26 (mouse ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of ssDNA were added to 95 μl of VSV-G-EVs in trehalose buffer and EVs then transferred to a 1 mm electroporation cuvette (BioRad) placed on ice ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was then heated up to 95°C for 5 minutes and cooled down to 30°C at the rate of 1°C per minute using a thermal cycler (BioRad).
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Bioengineering 2022Quote: ... droplet-digital PCR was performed for 16 extracted RNA samples using the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad) and the CFX96 thermocycler (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed three times and incubated with secondary antibodies: Donkey anti Sheep/Goat IgG (dilution 1:100,000, #STAR88P, Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... HRP-linked Goat αRat antibody (Pierce 31470) was used at 1:30,000 and detected with an HRP Substrate kit (Bio-Rad) and Bio-Rad ChemiDoc imager ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for one h at room temperature (RT) with horseradish peroxidase (HRP)-coupled secondary Abs at 1:2000 for one h (all secondary Abs from Biorad, raised in goatanti-rat HRP Cat # 5204-2504 and anti-mouse HRP Cat # 1721011) ...
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bioRxiv - Molecular Biology 2022Quote: ... The combined solution was incubated at room temperature for 1 hr and then mixed with BioBeads SM-2 resin (BioRad) overnight to remove the detergents ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... detergents and undigested protein were removed using a Bio-Scale Mini Macro-Prep High S 1 mL cartridge (Bio-Rad) on an Agilent 1100 HPLC system at 1 mL/min ...
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bioRxiv - Microbiology 2022Quote: ... Equivalent to 1×106 parasites were resolved by electrophoresis on a Mini-Protean TGX™ precast gel (Bio-rad, California). Lipoylated proteins were detected with rabbit anti-lipoic acid antibody (ab 58724 ...
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bioRxiv - Molecular Biology 2022Quote: ... the pellets were re-dissolved in 1 M NaOH and quantified by Bradford assay (Quick StartTM Bradford Kit, BIO-RAD), and the supernatants containing cADPR were neutralized with Chloroform/Tri-n-octylamine mixture (volume ratio 3:1 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of total RNA were treated with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; https://www.bio-rad.com/) for genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 1 µg RNA was used for cDNA synthesis with the IScript cDNA Synthesis Kit (Bio-Rad, 1708890), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were thawed on ice and diluted 1:1000 in 1X Bradford reagent (BioRad, Hercules, CA, Cat. No. 500-0006). Standard curve was determined via serial dilutions of 1 mg/mL BSA diluted in H2O ...
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bioRxiv - Biophysics 2024Quote: ... The concentration of TP 70 was determined by measuring A230 (1 A230 unit = 240 pmol TP70) by SmartSpec Plus spectrophotometer (BIORAD). Aliquots were flash frozen and stored at −80°C.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 0.5 μM of gene-specific primers (Sup Table 1) in a CFX96 qPCR Real-Time PCR module with C1000 Touch Thermal Cycler (BioRad). After 10 minutes at 95°C ...
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bioRxiv - Neuroscience 2024Quote: ... 72°C (30s) and 1 cycle of 72°C for 5 min (C1000 Touch Thermal Cycler, Bio-Rad, Hercules, CA). 2–5 μl of the PCR reactions were subjected to gel electrophoresis using a 3% agarose gel in tris-borate-ethylenediamine tetraacetic acid buffer ...
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bioRxiv - Physiology 2024Quote: ... Total RNA (1 μg) was reverse transcribed to cDNA using an iScript cDNA synthesis kit (Cat no. 170884, BioRad, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBST and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in 1X Tris-buffered saline (TBS) for 1 hour at room temperature prior to the addition of 0.05% Tween 20 (Bio-Rad) and primary antibodies overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... PVDF membranes were blocked for 1 hour in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4 °C with antibodies listed in Supplementary Table 2 in 5% BSA in TBS-T ...
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bioRxiv - Developmental Biology 2023Quote: ... and sample aliquots were diluted 1:50 in water before Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were normalized to 1 mg/mL in 0.1% Azo ...
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bioRxiv - Cell Biology 2023Quote: ... Additional antibodies used for the infected ALI cultures include: mouse anti-RSV Fusion protein (1:100, Cat# MCA490, Bio-Rad), rabbit anti-Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Immunology 2023Quote: ... and the cells were resuspended in 1 mL of PBS and counted using a TC20 automatic cell counter (Bio-Rad). 4 mL of PBS was added to the sample and spun at 200 ×g for ten minutes to remove any contaminating Ficoll ...
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bioRxiv - Plant Biology 2024Quote: ... prior to bombardment with 10 mg 1 μm tungsten beads treated with 5 μg purified plasmid DNA in a Helios gene gun (BioRad) at 1,550 PSI ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplification was performed in the presence of 1 mM of each oligonucleotide and iTaq Universal SYBR Green Supermix (Bio-Rad). Relative amounts of targets were calculated using the ddCT method ...
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bioRxiv - Immunology 2023Quote: ... Resultant cDNA at 1:100 dilution was subject to qPCR performed using iQ SYBR Green Supermix (BioRad, Cat. No. 1708880) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... Blocked membranes were incubated with HuCAL anti-bovine Mincle CRD antibodies (2.5 µg mL-1) before incubation with secondary goat-anti-human IgG F(ab’)2: HRP (STAR126P, Bio-Rad) antibody ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied to an equilibrated 1-ml StrepTrap HP chromatography column connected to the NGC Chromatography System (BioRad), washed with 20 CV of StrepA and elution was performed by applying a gradient concentration of StrepB (150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared assay plate was exposed to temperature increases from 25 ⁰C to 95 ⁰C with 1 ⁰C increments for 5 s each using a CFX Connect Real-Time PCR Detection System (BioRad). SYBR fluorescence was detected after every temperature increase ...
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bioRxiv - Microbiology 2023Quote: ... or a median 750 ng (for HIV-1) of genomic DNA was combined with ddPCR Supermix for Probes (no dUTPs, BioRad), primers (final concentration 900 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... PVDF membranes were blocked for 1 hr in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4°C with antibodies listed in Table S1 in 5% BSA in TBS-T ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins of the parasite pellets were extracted using lysis buffer (1 mM DTT, 2X Laemmli buffer (Bio-rad, 161-0737) and protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2023Quote: ... in 1× TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (BioRad). Subsequently ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed in 1x TBST with mild agitation before incubation with horseradish peroxidase-conjugated secondary antibodies diluted in blocking buffer (α- mouse 1:10,000 (170-6516; BioRad), α-rabbit 1:10,000 (170-6515 ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated 1 h with horseradish peroxidase-conjugated secondary antibodies (1:5,000) and the bands were visualized by chemiluminescence using the Clarity Western ECL substrate (Bio-Rad) and a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... These mixtures were then heated up to 95 °C for 5 minutes and slowly cooled to room temperature at the rate of 1 °C per minute using a thermal cycler (BioRad).
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bioRxiv - Molecular Biology 2023Quote: ... We then utilized 1 µg of the total RNA for the SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad). rRNA depletion was performed with riboPOOL for D ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed on a 1% agarose gel using VersaDoc 3000 Gel Imaging System (Bio-Rad, Hercules, CA, USA). The intensity of the bands was quantified by densitometric analysis using ImageJ 1.53k software (NIH ...
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bioRxiv - Microbiology 2023Quote: The cDNA was diluted to 1:20 and analysed using iQ5 Real time PCR detection system (BioRad, Hercules, California, USA) with SYBR Green detection system ...