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Citations for Bio-Rad :
6201 - 6250 of 7483 citations for Mouse Claudin 1 CLDN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... MNV or HAV RNA recovered by the ISO 15216-1 and PGM-MB or HAV antibody methods was quantified using the One-Step dd-RT-PCR Advanced Kit for Probes (Bio-Rad Laboratories, Ltd.) according to manufacturer’s instructions and as described previously (23 ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μg total RNA was reverse transcribed into cDNA in a reaction volume of 20 μl using the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad, 1725038). Real-time PCR reactions were performed on the resulting cDNA using the CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... 500 ng of total RNA were used in cDNA synthesis with iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, USA) while 700 ng of total RNA from the AT2 callus in LD and LL were used as an input ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the DC protein assay according to standard protocols provided by the kit manufacturer (Bio-Rad, Hercules, CA). Equal volumes of soluble protein supernatants were transferred to new tubes and subjected to in solution digestion ...
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bioRxiv - Cell Biology 2022Quote: ... The total amount of protein isolated from the weighed tissues was determined by using a protein assay kit (Bio-Rad, Hercules, CA, USA). Hydroxyproline content data are expressed as μg of collagen per mg of total lung protein (μg/mg).
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred into clean tubes and the protein concentration was determined using the Bradford protein assay kit (Bio-Rad Laboratories, USA). The protein extracts (20 μg ...
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bioRxiv - Biochemistry 2021Quote: Reverse transcription (RT) was carried out with 500 ng of total RNA using the iScript™ cDNA Synthesis kit (Bio-Rad, Hercules, CA). qRT–PCR was carried out with the iQ™SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... and up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 172-5038). Quantitative real time-PCR was performed using Go-Taq qPCR Master Mix (Promega ...
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bioRxiv - Genetics 2022Quote: ... and cDNA was synthesized by with iScript™ cDNA synthesis kit according to the directions of the manufacturer (Bio-Rad, Hercules, CA, USA). Quantitative PCR was performed using Power SYBR™ Green PCR Master Mix kit on a StepOne Plus real time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... One µg of DNase treated RNA was used to synthesize cDNA using iScriptTM Select cDNA synthesis kit (catalog#170-8896, Bio-Rad, Hercules, CA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Quality and quantity of retrieved RNA was assessed using the Experion automated electrophoresis system and a RNA StdSens Analysis kit (Bio-Rad Ltd., UK).
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bioRxiv - Microbiology 2020Quote: ... was used for quantification and all PCR reactions were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad Cat# 1864022). Primers used were previously described in (25) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Reverse transcription was performed with Onescript® cDNA synthesis kit (Abm) and qRT-PCR was performed using SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad) and a CFX96™ Real-Time System device (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Bio-Rad, Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... and 200 ng DNA were used to analyze KRAS codon 12/13 and 61 mutations with ddPCR KRAS G12/G13 and KRAS G61 Screening Kits and QuantaSoft Analysis Pro software (Bio-Rad, Hercules, CA) as described elsewhere63 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on a 10% SDS polyacrylamide gel and visualised with Silver Stain Plus Kit (Bio-Rad Laboratories AB, Sweden) according to manufacturer’s modified silver stain protocol.
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were normalized for protein concentration using a BCA assay kit (Thermo 23225) and mixed with Laemmli buffer (Bio-Rad 161-0737). 50 µg of the protein mix were run through a 4-20% Criterion TGX gel (Bio-Rad 5671093 ...
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bioRxiv - Plant Biology 2021Quote: ... The protein concentration of each of the five biological replicates was estimated using a Bradford protein assay kit (Bio-Rad Laboratories, Hercules, USA).
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bioRxiv - Microbiology 2020Quote: ... the outer membrane control proteins OmpA (α-OmpA was kindly provided by Prasadaro Nemani (45)) or MOMP (α-MOMP, MONOFLUO™ Legionella pneumophila IFA Test Kit, BioRad), the inner membrane control protein LepB (antibody kindly provided by Gunnar von Heinje (46) ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration in supernatant will be determined by Bradford assay using the Bio-Rad Protein Assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of total protein are separated on 8∼12% SDS–PAGE gels ...
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bioRxiv - Genetics 2021Quote: ... Thirty nanograms of cDNA per reaction were analyzed for semi-qPCR using the SsoAdvanced™ Universal SYBR® Green Supermix Kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was used for various assays and the protein content of the homogenate was measured using DC protein assay kit (Bio-Rad, Hercules, CA). Lactase activity was assessed using a Lactase Assay Kit (Cat# E-BC-K131-S ...
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bioRxiv - Immunology 2021Quote: ... First-strand cDNA were synthesized from total RNA using an iScript gDNA Clear cDNA synthesis Kit according to the manufacturer’s instruction (Bio-Rad, Hercules, CA, USA). Real-time PCR was performed using the StepOnePlus Real-time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Total AGT concentration in each sample was normalized by total protein concentrations measured using a DC™ protein assay kit (Bio-Rad 5000112).
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bioRxiv - Immunology 2020Quote: ... 30-50µg of protein per lane alongside a prestained molecular weight protein marker was separated on an 8% SDS PAGE gel prepared from SDS-PAGE kit (Beyotime, China) and electrotransferred to immunoblot PVDF membrane (BIO-RAD, CA, USA) for protein blotting ...
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bioRxiv - Neuroscience 2021Quote: ... was used from each sample for One-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad) in the StepOnePlus™ Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: ... Results were validated using qPCR experiments where mRNA was reverse transcribed into cDNA using the iScript cDNA synthesis kit (cat #1708891, Bio-Rad, Hercules, CA). SYBR Green based qPCR was performed on a CFX-Connect Real Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... We measured total protein concentrations of cell lysates with the BCA protein assay kit (Pierce, Cat#23225) and diluted 40 μg of each protein with XT sample buffer (Bio-Rad, Cat#1610791) and XT reducing agent (Bio-Rad ...
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bioRxiv - Genetics 2020Quote: ... and was quantified by using a QuantifilerR Human DNA Quantification Kit (Applied Biosystem, USA) on a Bio-Rad Real-time PCR System (Bio-Rad Laboratories, USA). The quantification process was performed per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed by using a HiScript II one-step qRT-PCR SYBR green kit (Vazyme) on a CFX96 real-time PCR detection system (Bio-Rad Laboratories, Inc.). The following primers were used ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 0.2-1 µg of RNA was pre-treated with DNAse and used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad, Irvine, CA). Quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated at 200V for 45 min and then transferred to a low-fluorescence PVDF membrane at 25V for 30 min using the Trans-Blot Turbo Transfer System and Kit (Bio-Rad, Cat. #1704275). Gels were activated by UV excitation using the Bio-Rad GelDoc XR+ System prior to transfer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reverse transcription was performed with Onescript® cDNA synthesis kit (Abm, #G236) and qRT-PCR was undertaken using SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad) and a CFX96TM Real-Time System device (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Neuroscience 2022Quote: ... First strand cDNA was prepared from 400 ng of total RNA and the complementary DNA strand was synthesized using iScript cDNA synthesis kit (Bio-Rad, #1708891, USA), according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cervicornis sperm samples from 2015 were brought to a final concentration of 3 x 107 cells ml-1 after the addition of Cell Suspension Buffer and 2% agarose using the Bio-Rad CHEF Genomic DNA Plug Kits (Bio-Rad, Hercules, CA). Genomic DNA plugs were processed according to the manufacturer’s protocol and stored at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... one-step reverse transcription droplet digital PCR (RT-ddPCR) assays were performed with the One-step RT-ddPCR Advanced Kit for probes (Bio-Rad Laboratories Ltd.), using primers and probes targeting 2’,5’-oligoadenylate synthetase 1 (OAS1) ...
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bioRxiv - Molecular Biology 2024Quote: The synthesis of cDNA was done from 100 ng RNA in 20 μl total reaction volume using iScript Reverse Transcription Supermix kit (Cat# 1708841, Bio-Rad, Hercules, USA), without any changes from themanufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... A total of 1000 ng of RNA was used for cDNA synthesis with the High-Capacity cDNA Reverse Transcription Kit (Bio-Rad, Hercules, CA). Real-time PCR was performed with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: Total protein lysates were prepared from the cells followed by protein quantification using the protein DC assay kit (Bio-Rad, Cat # 5000-001) as described previously (28) ...
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bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α was analyzed by using quantitative real-time PCR according to the Biorad iScript One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers (F/R) ...
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bioRxiv - Cancer Biology 2023Quote: PDEC cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and PCR amplification were performed in a single reaction using the One-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, CA, USA) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration in cell lysates were measured using a RC DC Protein Assay Kit (cat. no. 50000119; Bio-Rad Laboratories, Hercules, CA, USA). Immunoblotting was carried out using standard procedures ...
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bioRxiv - Physiology 2023Quote: One hundred nanograms of total RNA was reverse-transcribed (RT) and amplified using the iScript One-Step RT-PCR kit for probes (Bio-Rad, Hercules, CA). Real-time qPCR was performed with the Bio-Rad CFX96 sequence detection system using predesigned primer/probe sets against CPT1a ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA was extracted from a liver of an adult female ferret using the CHEF Mammalian Genomic DNA Plug Kit (BioRad, Cat. No. #1703591). In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit). Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN ...
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bioRxiv - Cancer Biology 2023Quote: Messenger RNA expression was assessed with 150 to 500 ng of total RNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, USA, #1708891) according to manufacturer’s instructions ...