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Citations for Bio-Rad :
6101 - 6150 of 6365 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cell culture supernatants were screened for the presence of 27 human cytokines and chemokines using the Bio-Plex Pro Human Cytokine Standard 27-Plex kit (Bio-Rad Laboratory) on a FLEXMAP 3D® analyzer (Luminex ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified and normalized PCR products were further pooled into a single library and quantified using qPCR (KAPA Library Quant Kit qPCR Mix, Bio-Rad iCycler). The final library was diluted to 4 nM and sequenced in an Illumina HiSeq 2500 Platform using a 2×250bp RapidRun kit for an average coverage of 160,000 paired reads per PCR product.
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bioRxiv - Immunology 2020Quote: ... Immunoblots were conducted using Mini-Protean TGX stain-free gels and Transfer-Blot Turbo RTA Transfer Kit reagents(Bio-Rad, Mississauga, Ontario). ABCF1 protein was probed with primary anti-ABCF1 antibody (HPA017578 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA concentration was estimated by nanodrop and 1 μg of RNA was used to synthesize cDNA with iScript cDNA synthesis kit™.(Biorad 1708890) qPCR was performed using Precision™ 2X qPCR master mix (Primerdesign ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 μg of RNA was reverse transcribed into cDNA using BUNiScriptTM cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 170-8891). For pancreas samples ...
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bioRxiv - Microbiology 2022Quote: ... The amplification kinetics of each product were checked at the end of each cycle by measuring the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cDNA was with Taq Pro Universal SYBR qPCR Master Mix kit (Q712, Vazyme, Nanjing, China) by the CFX96TM Real-Time System (BIO-RAD, USA). The thermal cycles were 95 ℃ for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The fixed and stained gene expression slide was treated with a permeabilization enzyme specific for mouse tongue determined by the Spatial tissue optimization slide kit (10x Genomics, #1000191) for 24 minutes on the thermal cycler (Bio-Rad, 1851197). Synthesis of cDNA and second strand were performed using reverse transcriptase and second strand reaction (primer and enzyme) ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from cardiac cultures after 4 days on either soft or stiff PDMS (n=4) using the AurumTM Total RNA Mini Kit (Bio-Rad Laboratories). Libraries were constructed by the Purdue Genomics Core Facilities according to standard protocols using TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Neuroscience 2019Quote: ... and cDNA was reverse transcribed from 1 ug of the resultant RNA with the Bio-Rad iScript kit (Bio-Rad 170-8891) in a reaction volume of 20 µl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Biorad, Mississauga, ON Canada). Quantitative real-time PCR (qPCR ...
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... Complimentary DNA (cDNA) synthesis was conducted using the iScript™ cDNA Synthesis Kit with 500 ng of starting RNA (Bio-Rad Laboratories), and combined with 50 μM of forward and reverse primers (for primer sequences ...
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bioRxiv - Cancer Biology 2019Quote: ... Isolated RNA (100 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) performed on the 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... and ampR was measured by quantitative Reverse Transcriptase PCR (qRT-PCR) using the iTaq Universal SYBR Green One-Step kit (Biorad, Hercules, CA) and a CFX96 Touch Real-Time PCR detection system (Bio- Rad ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was washed three times with TBST and detection was performed with the Clarity™ Max ECL kit for pulldown experiments (Bio-Rad) and Clarity™ ECL kit for all other experiments (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... This step was repeated two times and the protein concentration was determined by the DC Protein Assay Kit (Bio-Rad, Munich, Germany) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA was synthesized from 500 ng of RNA using the iScript kit according to the manufacturer’s protocol (Bio-Rad, 170-8891).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Biorad, Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was then generated using the Superscript™ III Reverse Transcriptase kit and pre-amplified with a mixture of the tested TaqMan® probes (Table S1) using the SsoAdvanced™ PreAmp Supermix (Biorad). Differential levels of cDNA expression were measured using the droplet digital PCR (ddPCR ...
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bioRxiv - Microbiology 2019Quote: ... and FLAG-cMCF-HA recovered as described above were prepped using the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio-Rad 1632130). The sample was then loaded onto 11 cm pH 3-10 immobilized pH gradient (IPG ...
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bioRxiv - Molecular Biology 2019Quote: ... reverse transcription (RT) was carried out with 1 µg of total RNA using the iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was performed by using a Universal SYBR® Green kit and the C1000 Touch Thermal Cycler real-time PCR detection system (Bio-Rad). The EF1α (AT5G60390 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from RNA isolated from the cell lysates using a iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA), and qRT-PCR was conducted as described previously (21 ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 100 ng RNA per sample was subjected to Reverse Transcriptase (RT) by using iScriptTM cDNA Synthesis kit (1708890, BioRad, Hercules, CA). For whole liver ...
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bioRxiv - Cell Biology 2021Quote: ... and 10-14h after egg laying and reverse transcribed using oligo-dT primers with a One-Step RT kit (Bio-Rad 1725140). The coding sequence for each gene encoding a gene of interest was amplified from a cDNA template using Phusion High Fidelity DNA polymerase (Thermo Fisher F-530XL) ...
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bioRxiv - Physiology 2020Quote: ... The total RNA was purified from genomic DNA contamination then converted to cDNA using iScriptTM gDNA Clear cDNA synthesis kit according to manufacturer’s instructions (Bio-Rad, CA, USA). Commercially available SYBR® Green Glucose Metabolism Array consisting of 84 genes were used (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Densitometry of the blots was detected using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA). Information on the primary antibodies used has been listed and provided (Table EV4).
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was exposed using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA).
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bioRxiv - Plant Biology 2021Quote: The cDNA synthesis was performed using 1 μg of total RNA and iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 μg of proteins per sample were resolved in 10 or 12% gels (TGX™ FastCast™ Acrylamide kit, Bio-Rad) via electrophoresis ...
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bioRxiv - Genomics 2021Quote: ... Reverse transcription (RT) was performed on 1 μg of DNase-treated total RNA using the iScript cDNA synthesis kit (Bio-Rad #1708890), with the same amount of total RNA diluted in water as negative controls for RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... The product from the final elution and whole cell lysate supernatant were subjected to silver staining using Silver Stain Plus Kit (BIO-RAD, # 1610449) to check pull down efficiency and purity ...
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bioRxiv - Immunology 2021Quote: ... 30 μg of protein was mixed with an equal volume of 2×Laemmli buffer supplemented with 5% 2-mercaptoethanol (Catalog no. 1610737) then loaded in 10% polyacrylamide precast gel (TGX™ FastCast™ Acrylamide Starter Kit, 10%, catalog no. 1610172) from BioRad Laboratories (Mississauga ...
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bioRxiv - Molecular Biology 2021Quote: ... and quantitative PCR (qPCR) was performed to quantify mRNA abundance using a SsoFast™ EvaGreen® Supermix kit (Bio-Rad, 172-5204,) on a Bio-Rad CFX96 cycler ...
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bioRxiv - Immunology 2020Quote: ... Multiplexed cytokine measurements on macrophage cell culture supernatants were performed using a custom Bio-Plex Express Human Cytokine kit (Bio-Rad 17004073) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 30 µg of protein was mixed with an equal volume of 2×Laemmli buffer supplemented with 5% 2-mercaptoethanol (Catalog no. 1610737) then loaded in 10% polyacrylamide precast gel (TGX™ FastCast™ Acrylamide Starter Kit, 10%, Catalog no. 1610172) from BioRad Laboratories (Mississauga ...
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bioRxiv - Cell Biology 2021Quote: ... Cytokine secretion assay was performed using ThermoFisher ProcartaPlex Multiplex Custom Made mouse cytokine 5-plex assay kit following manufacturer’s instructions and analyzed with the Bio- Plex 200 Luminex-based multiplex analysis system (Bio-Rad, Hercules, CA). All samples were analyzed using technical duplicates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Reverse transcription reactions were performed to synthesize cDNA from 200 ng of total RNA using the iScript™ Select cDNA Synthesis Kit (Biorad, CA). Real-time quantitative PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... An aliquot was taken to measure radioactivity and protein content using a liquid scintillation counter and a DC protein assay kit (Bio-rad, USA), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were stained using the Silver Staining Plus Kit and documented on the ChemiDoc XRS+ system (Bio-Rad Laboratories Inc., Hercules, USA) according to the manufacturers protocol.
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bioRxiv - Genomics 2020Quote: ... the membrane was incubated with peroxidase-conjugated goat anti-mouse IgG and proteins were detected using a chemiluminescence detection kit (Super-Signal West Pico, Pierce) and a ChemiDoc™ MP system (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad, Marnes-la-Coquette, France). The membranes were blocked for 30□min in 5% low-fat milk in TBS-0.1% Tween 20 at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The immunoblotting signals were detected using a CCD camera (Intas Biopharmaceuticals, Ahmedabad, India) after application of enhanced chemiluminescence detection kit (Clarity™ Western ECL Substrate; Bio-Rad, Hercules ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR analysis were performed with iQ-SYBR Green Supermix kit in an iCycler iQ5 system following the instructions of the manufacturer (Bio-Rad, USA). The relative quantifications of mRNA levels were performed using the cycle threshold (Ct ...
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bioRxiv - Synthetic Biology 2022Quote: ... #FABRK 001-1) and 400 ng of cDNA was synthesized using the iScript™ Select cDNA Synthesis Kit (Bio-Rad Laboratories, #1708897) on a Mastercycler® nexus PCR Thermal Cycler (Eppendorf) ...
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Germany) and 1 μg of total mRNA was translated to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according to the instructions.
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bioRxiv - Immunology 2023Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers/probe mixtures (all from Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 500 ng of total RNA was used in a 20 µL reaction volume with the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, #1708890) according to the manufacturer’s protocol ...