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Citations for Bio-Rad :
6051 - 6100 of 6365 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected using the EZ-ECL Chemiluminescence Detection Kit for HRP (Biological Industries) and ChemiDoc XRS+ Gel Documentation System (Bio-Rad Laboratories). Images were taken using the ImageLab™ software (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 1 μg of RNA was subjected to retrotranscription using iScript™cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 400 ng of complementary DNA (cDNA) was synthesized using the reverse transcriptase iScript complementary DNA synthesis kit (Bio-Rad, Hercules, CA; # 1708891), then diluted to a concentration of 2 ng/μL ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: 250 ng of total RNA of each sample was reversely transcribed and amplified using the TargetAmp-Nano Labeling Kit for Illumina Expression BeadChips (Biozym Scientific GmbH, Hessisch Oldendorf, Germany) on a Biorad MJ Thermal Cycler (Biorad, Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... CLN5 transcripts were measured from the control and CLN5i neurons RNA by quantitative polymerase chain reaction[13] using an iTaq Universal Probes one-step kit (Bio-Rad, 1725141) on a Roche 96-well thermocycler ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaned samples were solubilized in the previous buffer described above and then quantified with the RCDC Protein Assay kit (Biorad, Hercules, CA). Ten μg of protein from each sample were digested in-solution using both Lys-C and Trypsin ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific) and a T100™ thermal cycler (Bio-Rad). The expression of selected genes was determined by RT-PCR using the iQ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using the Bio-Rad CFX Manager software ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolated RNA (50 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) in the 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2019Quote: ... First strand synthesis and real time PCR were performed using iScript kit and iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA) on Light Cycler 480 (Roche ...
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bioRxiv - Genomics 2019Quote: Mammalian cells were embedded in gel plugs and High Molecular Weight DNA was purified as described in a commercial large DNA purification kit (BioRad #170-3592). Plugs were incubated with lysis buffer and proteinase K for four hours at 50°C ...
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bioRxiv - Immunology 2019Quote: ... One microgram of RNA was used to synthesize first strand cDNA using the iScript cDNA synthesis kit from Bio-Rad (Hercules, CA). The primers used for qPCR were synthesized by IDT (Coralville ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. #: 1708890). cDNA was diluted to 20 ng/µl and 2.5 µl/reaction was used as input for the qPCR reaction ...
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bioRxiv - Cancer Biology 2019Quote: One microgram of total RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc., Hercules, CA). The PCR reactions were performed with 0.2 µl of cDNA template in 25 µl of reaction mixture containing 12.5µl of iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2019Quote: ... Gene silencing efficiencies were performed as described previously18 with the following modifications: cDNA was synthesized from 1 μg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, #1708891) following the manufacturer’s instructions and qRT-PCR was performed with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Quantification of gene expression was performed by using the KAPA SYBR-FAST One-Step qRT-PCR kit and the CFX-Connect Real-time PCR detection system (Bio-Rad Laboratories) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and total RNAs were obtained according to the manufacturer’s protocol and then quantified and synthesized into cDNA using a iScript cDNA Synthesis Kit (Bio-Rad, Hercules, USA) for SALL4 expression or using a High-capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... we synthetized 100ng of cDNA from immunoprecipitated and isolated polyribosome-associated RNA (see above for detailed procedure) as well as non-precipitated RNA (input) using the iScript cDNA synthesis kit (Bio-Rad, USA). cDNA was then preamplified using TaqMan assay following kit instructions46 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were reverse transcribed from 500 ng of total RNA in a 20 µL volume using iScript cDNA synthesis kit (Bio-Rad Laboratories). Real-time qPCR was carried out on a C1000™ Thermal Cycler (Bio-Rad Laboratories) ...
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bioRxiv - Genomics 2019Quote: ... 1 μg of each RNA sample was converted into single-strand cDNA with random hexamers using an iScript™ Select cDNA Synthesis Kit (Bio-Rad). To validate fusion transcripts ...
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bioRxiv - Neuroscience 2020Quote: ... Up to 1 μg of RNA was used to synthesise cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) and PowerUp SYBR Green (Thermofisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... the homogenate was centrifuged at 16,000g for 5 min and 5 µl of the supernatant was used for protein measurement in a microplate format utilizing the Lowry DC protein assay kit and γ-globulin for generating standard curve (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... PCR was performed using the AceQ qPCR SYBR Green Master Mix kit (Vazyme, Nanjing, China) and the MyiQ2 Real-time PCR system (Bio-Rad, USA) with the following cycling conditions ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatants were transferred into a new set of tubes and protein concentration was determined using Bio-Rad DC™ protein assay kit (Bio-Rad). Western blot protocol is the same as described before ...
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bioRxiv - Cell Biology 2021Quote: ... 25 ng of cDNA template was mixed with the primers to final concentrations of 200 nM and mixed with 5 μl of iTaqTM Universal SYBR® Green Supermix kit (Bio-Rad). The reactions were done in triplicates with the following conditions ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of RNA was used to generate cDNA with the iSCRIPT Clear kit (Bio-Rad, Hercules, CA, cat# 170-8891). For the qRT-PCR ...
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bioRxiv - Biochemistry 2021Quote: ... Quantitative cDNA detection was performed via quantitative real-time PCR (RT-qPCR) with the same gene-specific primers using the iQ™ SYBR® Green Supermix Kit (BIORAD). Serially diluted genomic DNA was used as a quantification standard.
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bioRxiv - Biochemistry 2021Quote: ... For analysis of isolated RNA semi-quantitative reverse transcription–polymerase chain reaction (RT) PCR was performed with the iScript™ cDNA Synthesis Kit (BIORAD, Germany) according to the manufacturer’s instructions using 500ng of isolated RNA ...
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bioRxiv - Cell Biology 2021Quote: ... in accordance with the manufacturer’s protocol then performing qPCR using a Sangon Biotech microRNA qPCR kit with SYBR Green (B532461) in a Bio-Rad CFX96™ real-time PCR detection system (Bio-Rad) using the following thermocycling conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Q-PCR was performed on a Bio-Rad IQ5 real-time PCR detection system by using iTaq Universal SYBR Green One-Step RT-qPCR kit (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral or control antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a new tube and protein concentration was measured with a DC Protein Assay Kit (Bio-Rad, 5000111) using a photospectrometer at 750 nm (Molecular Devices ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was subjected to SYBR Green qPCR using iTaq™ Universal SYBR Green Supermix kit from Bio-Rad (Hercules, CA, US) and primers specific to the 5’-end of tRFs and RNA linker ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram was reverse transcribed to generate first-strand complementary DNA (cDNA) using IScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA). qRT-PCR experiments were performed with 2.5 μl of a 1:20 dilution of first-strand cDNA and LightCycler 480 SYBR Green I Master mix ...
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bioRxiv - Plant Biology 2021Quote: ... and treated aliquots amounting to 10 ng RNA were used for cDNA synthesis (iScript™ cDNA Synthesis Kit, BioRad, Hercules, CA, USA). Quantitative real-time PCR was carried out using a StepOnePlus (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from freshly isolated or cultured cells using the Aurum Total RNA Mini Kit (732-6820, Bio-Rad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The soluble protein content was spectrophotometrically determined using bovine serum albumin as a standard (Bradford Protein Assay Kit; Bio-Rad, Hercules, CA).
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bioRxiv - Molecular Biology 2022Quote: ... Signals were visualized with reagents included in the Efficient Chemiluminescence Kit (Vigorous, Beijing, China) and ChemiDoc XRS (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Animal Behavior and Cognition 2022Quote: Aliquots of the samples were organized in a 96well PCR plate and reverse transcribed to cDNA using the iScriptTM Reverse Transcription Supermix kit and protocol (BioRad; Hercules, CA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... equal amounts of protein were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a PVDF membrane by Trans-Blot® TurboTM RTA Mini PVDF Transfer Kit (Biorad, USA). Later ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad) and stored at −20 °C overnight ...
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bioRxiv - Plant Biology 2021Quote: The cDNA synthesis was performed using 1 μg of total RNA and iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: cDNA was synthesized using 1 μl of total RNA from melanocytes or iridophores with iScript™ Advanced cDNA Synthesis Kit (#1725038, Bio-Rad) following by 6 cycles of pre-amplification with pooled 47 pairs of MTE primers designed for NanoString CodeSet (SsoAdvanced™ PreAmp Supermix ...
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bioRxiv - Microbiology 2020Quote: ... All gene transcripts were quantified by quantitative PCR using qScript™ One-Step qRT-PCR Kit (Quanta Biosciences, #95057-050) on CFX96 real-time PCR system (Bio-Rad). Primer sequences for qPCR were as follow ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were quantified using the Kapa SYBR FAST Universal qPCR kit for Illumina sequencing on the CFX384 Real-Time PCR Detection System (Bio-Rad Laboratories). The libraries were diluted to 4 nM stocks and pooled equitably for sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription (RT) was performed using 250 ng of DNase-treated RNA using the iScript cDNA synthesis kit (Bio-Rad Cat#1708890) and the resulting RT reactions were diluted 1:5 with nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed and homogenized as described elsewhere.30 Protein concentration was determined using a DC protein assay kit (Bio-Rad, 5000116). 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad) ...