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Citations for Bio-Rad :
6051 - 6100 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... supernatants or virions were separated on 10% SDS polyacrylamide gel under reducing conditions and transferred to a nitrocellulose membrane with a Trans-Blot Turbo System (Bio-Rad, Hercules, CA, USA). Filters were saturated for 1 h with 5% nonfat dry milk in TBST (TBS with 0.1% Tween 20 ...
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bioRxiv - Genomics 2021Quote: When cDNA was used as a template the 20 μl ddPCR mix consisted of 10 μl ddPCR Supermix for Probes (Bio-Rad Laboratories, Munich, Germany), 1 μl 20x UBC primer and probe mix (Table 3 ...
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bioRxiv - Biochemistry 2022Quote: Protein overexpression and purification were analysed via 10% SDS-PAGE and imaged via ChemiDoc™ MP Imaging System (Bio-Rad; Fig. S1 and S4). Immunoblotting was performed to confirm purification results with primary anti-6xHis polyclonal mouse antibody (Beyotime® ...
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bioRxiv - Neuroscience 2022Quote: ... was prepared by mixing the following reagents in a total volume of 20 µl: 2x QX200 ddPCR EvaGreen supermix (Biorad, catalog #1864036, 10 µl), forward and reverse primers (10 µM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An equal amount of total protein was resolved by a 10% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked and incubated with either anti Phospho-(Ser/Thr ...
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bioRxiv - Molecular Biology 2022Quote: 20µg of protein extracts or 10% Co-IP samples were resolved on 10-12% SDS–PAGE and proteins were transferred to PVDF membranes using a semi dry Western blot system (Bio-Rad Trans-Blot SD). Membranes were blocked in 5% non-fat milk in PBS-T (1x PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and transferred to PVDF transfer membrane (IPVH00010 Immobilon®-P; MilliporeSigmaTM, Burlington, USA) overnight at 10 mA in a cold room (Mini trans-blot cell; Bio-Rad, Hercules, USA). The membrane was blocked for non-specific sites and incubated with anti-HvHsfA6a in 1:2000 dilutions overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The resolved proteins were transferred on to a 0.22 µ nitrocellulose membrane (#10600004, Cytiva, US) for 60 min at 10 V using the Trans-blot semi-dry transfer apparatus (Bio-Rad Laboratories Inc., US). The blots were blocked with 4 % skimmed milk (#2325 ...
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bioRxiv - Genetics 2024Quote: ... The cDNAs were individually diluted 10-fold for use as templates for subsequent qPCR with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, USA, 1725124). Mouse Gapdh was used as an internal control ...
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Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerancebioRxiv - Plant Biology 2022Quote: ... coated with 5 mg of plasmid DNA for editing rice STOMAGEN were divided equally among 10 macro-carriers and used for bombardment with a Bio-Rad PDS-1000 He biolistic device (Bio-Rad, Hercules, CA, USA) at 650 psi ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: Cell or tissue lysates (10–30 μg protein) or immunoprecipitated samples were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against leptin (Cat# ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 3.6 μL of ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The expression of the housekeeping gene GADPH (XM393605 ...
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bioRxiv - Neuroscience 2023Quote: ... the incubated membranes were washed again three times with 1x TBST for 10 minutes each at RT and visualised with Clarity Western ECL Substrate (BIO-RAD, Cat. #170-5060).
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were obtained from MAYV- or UNAV-infected HMC3 or HBEC-5i cells in Laemmli buffer with 10% dithiothreitol (Bio-Rad, Hercules, CA, USA). Proteins were fractionated in SDS-PAGE ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... DNA was diluted to 10 ng/μl and 2 μl were used in each RT-qPCR reaction with 10 μl of iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA) and 400 nM of forward and reverse primers ...
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bioRxiv - Immunology 2023Quote: ... Leaves were kept in the dark for 10 min before luminescence was detected with a ChemiDoc™ XRS+ imagine system (Bio-Rad, Feldkirchen, Germany). Luminscence intensities/mm2 infiltrated leaf area of different combinations were evaluated using the Image Lab software (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... The elutions were then concentrated on an Amicon spin column (10 kDa cutoff) and quantified by the DC assay (Bio-Rad, Hercules, CA, USA). The eluted protein was then diluted in PBS (pH 7.4) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were separated using 10% SDS page and blotted on a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, Inc., Austria). The membrane was probed with the anti pY-4G10 (mouse ...
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bioRxiv - Evolutionary Biology 2023Quote: ... real-time PCR was performed in a reaction volume of 20 μL comprising 10 μL Mastermix with SYBR green (Bio-Rad, Hercules, CA, USA), 0.6 μL each primer (1.2 μM) ...
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bioRxiv - Microbiology 2024Quote: ... A 40 µg of protein of CFS samples and 10 ng of purified rGdhA were resolved in a 4-20% SDS-PAGE (Bio-Rad, Hercules, CA; # 4561094) and immunoblotted with affinity purified anti-rabbit rGdhA antibody (0.3 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... at 95°C for 10 mins before being loaded into 4-20% Mini-PROTEAN® TGXTM Precast Protein Gels (15 wells, 15uL; Bio-Rad, CA, USA) and subject to electrophoresis on a Bio-Rad Mini-PROTEAN® Tetra Cell system (180 V ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were then separated by electrophoresis on a 10% SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Cat. No. 1620177). The membrane was blocked with 5% bovine serum albumin (B.S.A. ...
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bioRxiv - Microbiology 2024Quote: ... resolved on the 10% SDS PAGE and transferred to nitrocellulose membrane (Trans-Blot Turbo Midi Nitrocellulose Transfer Pack, Bio-Rad, 0.2 µm pore size) with the use of a Trans-Blot Turbo Transfer Starter System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... 10*106 virions of IMP-1088 or DMSO released purified viruses were lysed in 4x Laemmli protein sample buffer (BIO-RAD, Cat. no. 1610747). Samples were electrophoresed on 4-20% precast polyacrylamide gel (BIO-RAD ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time quantitative polymerase chain reactions (RT-qPCR) were performed in 10 μl reactions containing Power SYBER Green PCR master mix (Bio-Rad; Cat #: 172-5121), 5 ng of cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein (20 μg/lane) were resolved in 10% Bis-Tris Criterion™ XT Precast Gels and XT MOPS running buffer (BioRad, Cat# 1610799, USA) at 120 volts for 1.5 h using Criterion™ vertical electrophoresis cell (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (10 µg of protein per well) were separated on SDS-polyacrylamide gels (CriterionTM TGXTM Precast Gels, 4-15%; Bio-Rad, Hercules, CA, USA) using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The collected protein samples were electrophoresed on 10% SDS-polyacrylamide gel (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Cat. No. 1620177). After blocking with 5% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: 20 µg of proteins were separated on a 10% polyacrylamide gel and transferred on nitrocellulose membranes (Amersham™ Protran®; #106000004) using Trans-Blot® Turbo™ (BioRad). Blocking was performed through a 1h incubation in 5% low fat milk in TBST ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were incubated in 1:1000 rabbit anti-HSP90 antibody (CST #4874) and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). Signal was revealed using Clarity Max™ Western ECL Substrate (Bio-Rad #170-5062) ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were incubated in 1:1000 rabbit anti-HSP90 antibody (CST #4874) and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). Signal was revealed using Clarity Max™ Western ECL Substrate (Bio-Rad #170-5062) ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... FITC-conjugated integrin β1 (CD29, HM beta 1-1, #MCA2298) and FITC-conjugated integrin β3 (CD61, #MCA2299) were from Bio-Rad (Oxford, UK). CHRONO-LUME® was from Chrono-log Corporation (Labmedics ...
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bioRxiv - Biophysics 2022Quote: The purified proteins of V2HeR3 were reconstituted into a mixture of POPE and POPG membranes (molar ratio = 3:1) with a protein-to-lipid molar ratio of 1:20 by removing DDM using Bio-Beads (SM-2; Bio-Rad, CA, USA). The reconstituted samples were washed three times with 1 mM NaCl and 2 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4°C and for 1 h at room temperature (RT) with secondary horseradish peroxidase conjugated antibodies (Bio-Rad, 1:3000). Membranes were then developed using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated antirabbit (1: 2500, 170-6515) or antimouse (1: 5000, 170-6516) IgG secondary antibodies (BioRad, Hercules, CA, USA). Proteins were detected using the SuperSignal chemiluminescent substrate kit (Pierce).
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bioRxiv - Physiology 2022Quote: ... The samples were prepared to contain equal concentrations (1 μg·μl-1) of protein in 1x Laemmli buffer (cat#1610747, Bio-Rad, Hercules, California, USA) and stored at −80°C for future analysis.
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bioRxiv - Cell Biology 2024Quote: ... 4.5 μL of repair template and 1 μg of pSI103-1 were mixed in a cuvette from Bio-Rad (Catalog No. 165-2086). The mixture was electroporated using ECM® 630 from BTX – Harvard Apparatus with the following conditions ...
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bioRxiv - Cell Biology 2024Quote: ... each lysate was diluted at a 1:1 ratio with a solution of 2× Laemmli sample buffer (#1610737EDU, Bio-Rad, Hercules, CA, USA), containing 5% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... 04404-84, Nacalai tesque), anti-APC (CC1, 1/100, OP80, Merk, Darmstadt, Germany) and rat anti-MBP (1/100, MCA409S, Bio-Rad, California, USA) in antibody dilution buffer (1% normal goat serum ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were diluted in blocking buffer (Iba1, 1:1000, Wako cat no 019-19741; CD68, 1:400, Bio-Rad cat no MCA1957) and incubated overnight at 4°C with mild agitation ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-5 µg of the non-denatured protein samples were diluted 1:1 with the native sample buffer (BIO-RAD #161-0738) and loaded into the polymerized gel ...
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bioRxiv - Plant Biology 2020Quote: ... Complementary DNA (cDNA) was synthesised using iscript cDNA synthesis Kit following the manufacture’s protocol (Bio-rad). The quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was reverse-transcribed into cDNA by using the iScriptTM cDNA Synthesis Kit (Bio-Rad #1708890) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was synthesized from RNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). qPCR was performed with the iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... and used as a template for cDNA synthesis using iScript™ cDNA Synthesis Kits (BIO-RAD). RT-PCR reactions were performed using SYBR Green Supermix (BIO-RAD ...
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bioRxiv - Cancer Biology 2021Quote: ... The total protein content was quantified using the DC protein assay kit (Bio-Rad, Richmond, CA). The proteins were denatured by incubation with 1 × Laemmli buffer containing β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was then created from the RNA samples using an iScript kit (BioRad, Hercules, CA, USA). After diluting cDNA 1:10 in distilled water ...
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bioRxiv - Cell Biology 2019Quote: ... 1.0 μg RNA was reverse-transcribed with the iScript cDNA Synthesis kit (1708891BUN, Bio-Rad Laboratories) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... were used to probe the proteins of interest by an ECL kit (Bio-Rad, cat#1705060). Two ECL-based western blot detection methods were utilized ...