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Citations for Bio-Rad :
6001 - 6050 of 7138 citations for 7 Chloro 4 hydroxy 1 8 naphthyridine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... at 400 mA for 1 hr on a Trans-blot SD semi-dry transfer cell (Bio-Rad). The HSF-1-HSE DNA complexes were visualized by autoradiography using LightShift Chemiluminescent EMSA kit (Thermo Fisher).
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bioRxiv - Biochemistry 2019Quote: ... and for AAC1 the primary antibody was 1:5000 dilution of rabbit IgG anti-AAC1 (Bio-Rad). The secondary antibody was 1:5000 dilution of horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2019Quote: ... Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891). Intron-spanning primers and probes for cep290 and ef1a1l1 were designed with Primer3Plus (http://primer3plus.com) ...
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bioRxiv - Pathology 2020Quote: ... and specific primers (Table 1) were performed using a CFX Connect Real-Time PCR Detection System (BioRad). Relative expression of mRNAs was determined using the software CFX Maestro (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was generated from 1 μg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad). Assays were performed with PerfeCTa SYBR Green SuperMix ...
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bioRxiv - Plant Biology 2021Quote: ... 1-2μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis kit (BioRad) or StarScript II First-strand cDNA Synthesis Kit (GeneStar) ...
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bioRxiv - Genomics 2021Quote: ... before in situ reverse transcription and annealing of barcode 1+linker on a thermocycler (Bio-Rad T100). After RT ...
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bioRxiv - Immunology 2021Quote: ... cDNA was diluted 1 μg / 100 ul and qPCR was performed on a CFX96 Instrument (Bio-Rad) using SYBR Green incorporation ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized from 1 μg RNA using the iScript Advanced cDNA Synthesis Kit (Bio-Rad, #1725037). For the 3-12 mo trial ...
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bioRxiv - Physiology 2020Quote: ... but on a 10% Mini-Protean® TGX™ Precast Protein Gel (Supplemental Figure 1, BioRad, 4561036). Because of its limited use ...
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bioRxiv - Cell Biology 2021Quote: Mouse monoclonal anti-Viral V5-TAG antibody (1:5000; clone SV5-Pk1) was purchased from Bio-Rad/AbD Serotec ...
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bioRxiv - Molecular Biology 2021Quote: 1 μg Turbo DNase-treated RNA was reverse transcribed with the iScript cDNA synthesis kit (BioRad, 1708890), treated with 0.5 μL RNase cocktail (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were then blocked for 1 h at 25 °C with 5% non-fat dry milk (BioRad) in TBST (50 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... followed by streptavidin-alkaline phosphatase (1 µg/mL) and development with AP conjugate substrate kit (Bio-Rad). For analysis of intracellular cytokine production ...
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bioRxiv - Immunology 2021Quote: ... Expression of target and housekeeping genes (see Table 1) was analysed on CFXTM Manager (Bio-Rad Laboratories) using the “Gene Study” function ...
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bioRxiv - Microbiology 2021Quote: ... each cDNA was combined with segment-specific primers (Supplementary Table 1) and Precision melt supermix (Bio-Rad) and analysed by qPCR using a CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... and transferred for 1 hour at 15V using Trans-Blot® SD Semi-Dry Transfer Cell (BioRad). The PVDF membrane was blocked with 5% milk in Tris-buffered saline with 0.1% tween-20 (TBST ...
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bioRxiv - Developmental Biology 2022Quote: 1 µg of RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Cat# 1708891, Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using iScript reverse transcription supermix (Bio-Rad, Hercules, CA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 1 ug of RNA using the iScript cDNA Synthesis Kit (BioRad #170-8891). Reverse Transcriptase Quantitative PCR (RT-qPCR ...
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bioRxiv - Cancer Biology 2022Quote: ... Each column then received 200 µL of ProteOn 1 M Ethanolamine hydrochloride pH 8.0 (BioRad, Cat. # 1762450) and was incubated for 4 hours to quench any remaining active site on the resin ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was mixed with sample loading buffer supplemented with 1% v/v 2-mercaptoethanol (BioRad, Cat #1610710). Sample was not heated to prevent aggregation of the GPCR ...
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bioRxiv - Genomics 2022Quote: ... 20 µg total protein extract per sample were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was dried for 1 h at 80 °C on a Gel Dryer 583 (Bio-Rad) and visualized after appropriate exposure on a Phosphorimager (FLA-3000 Series ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 h at room temperature in 5% skim milk (Catalog #170-6404, Bio-Rad). Primary antibody incubations were performed overnight at 4°C with antibodies diluted in TBS/0.1% Tween-20/5% BSA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Primary antibodies were labeled with horseradish peroxidase-conjugated secondary antibody (1:5000) and ECL substrates (Bio-Rad). The densitometric intensities of the bands were quantified by using ImageJ software.
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bioRxiv - Molecular Biology 2024Quote: ... This was followed by incubation with a secondary horseradish peroxidase-conjugated α-mouse antibody (BioRad – 1:2,000) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies at 1:10,000 dilution (Bio-Rad #172-1011 or 172-1019 ...
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bioRxiv - Microbiology 2024Quote: ... The reaction mixtures (20 μl) consisted of 1 × iQ SYBR green Supermix (Bio-Rad, Hercules, CA, USA), 0.5 μM of each primer ...
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bioRxiv - Molecular Biology 2024Quote: ... and transferred onto PVDF membranes using a wet blot system for 1 h at 100 V (Biorad). Membranes were blocked with BSA and incubated with primary antibody overnight at 4° C ...
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bioRxiv - Molecular Biology 2024Quote: ... The levels of proteins bound to chromatin were quantified using Quantity One 1-D Analysis Software (BioRad) and normalized with their corresponding Histone H2B values ...
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bioRxiv - Molecular Biology 2024Quote: ... we immunoblotted against SelN at a 1:500 dilution and rhodamine labeled anti-tubulin antibody (Bio-Rad) at 1:5,000 concentration ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... The gel was dried at 80°C for 1 h on a Gel Dryer 583 (Bio-Rad), exposed over night ...
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bioRxiv - Microbiology 2024Quote: ... and human proinflammatory cytokines were quantified using the Bio-Plex Pro™ Human Inflammation Panel 1 (BioRad). Fluorescence was read using a MAGPIX System (Luminex ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Synthesized cDNA was diluted 1:10 and 5 µl mixed with SsoAdvanced Universal Sybr Green supermix (Biorad). qPCR was performed using the Biorad CFX96 PCR machine ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies used for immunoblots were StarBright Blue 520 goat anti-mouse (IgG; 1:10,000; Bio-Rad) and Horseradish peroxidase conjugated-goat anti-mouse (IgG ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Biochemistry 2024Quote: ... and the band intensities were quantified and analyzed using Quantity One 1-D software (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2024Quote: ... HRP signals were visualized by enhanced chemiluminescence (ECL) (Cytiva) (1:25000) and imaged with Chemidoc (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: 1 μg Turbo DNase-treated RNA was reverse transcribed with the iScript cDNA synthesis kit (BioRad, 1708890), treated with 0.5 μL RNase cocktail (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg total RNA was subjected to reverse transcription with the iScript cDNA synthesis kit (Bio-Rad). In case of RNA isolated with TRIzol ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 µg RNA was used to synthesize cDNA using the iSCRIPT cDNA kit (Biorad, Hercules, USA) according the manufacturers description.
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 1 µg RNA using iScript gDNA Clear cDNA Synthesis Kit (BIORAD, Hercules, CA), according to manufacturer’s instructions and the cDNA was diluted to 5 ng/µl.
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bioRxiv - Immunology 2023Quote: ... RNA (0.5 to 1 μg) was reverse transcribed to cDNA using iScript cDNA Synthesis Kit (Bio-Rad), and real-time PCR reactions were performed as described previously10 ...
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bioRxiv - Neuroscience 2023Quote: ... mixed plasmid DNA (50 μg total) was coated on 1 μM-diameter gold particles (Bio-Rad, 1652263) in 0.5 mM spermidine ...
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bioRxiv - Neuroscience 2023Quote: 1 to 0.5 μg of total RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). A volume corresponding to 5 ng of initial RNA was employed for each real-time PCR reaction using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... the blots were incubated in and hFAB™ Rhodamine Anti-Actin (BioRad #12004163 at 1:2000 dilution). All experiments were performed in triplicate.
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized from 1 μg of total RNA using the iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using the LightCycler 480 (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... stock solution of 40% neutral acrylamide and N,N’-methylenebisacrylamide in a 19:1 ratio (Bio-Rad) were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt ...