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Citations for Bio-Rad :
5951 - 6000 of 7483 citations for Mouse Claudin 1 CLDN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and anti-tubulin Rhodamine antibody (AbD22584, 1:5000, Bio-Rad Laboratories, Hercules, CA, USA) were used for immunofluorescence detection ...
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bioRxiv - Immunology 2023Quote: ... followed by overnight incubation with F4/80 antibody (Clone CI:A3-1, Bio-Rad Laboratories) diluted in DAKO antibody diluent (1:100 ...
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bioRxiv - Immunology 2023Quote: ... 2× ddPCR supermix for probes (no dUTP) final concentration 1× (Bio-Rad Laboratories, USA), a 20× target primer/FAM-labeled probe mix ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 1 μg was boiled for 10 min in 1x Laemmli Sample Buffer (BioRad) with 5% 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibody used was goat anti-rabbit-HRP (1:5000, Bio-Rad, 170-6515). Ponceau-S red staining was used for mESCs samples as a loading control.
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bioRxiv - Microbiology 2023Quote: ... or goat anti-rabbit coupled with StarBright Blue 700 (Bio-rad, dilution 1:5000) and washed with PBST solution ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour at room temperature and imaged by the ChemiDoc Imaging System (Biorad). Images were analyzed and processed using Image Lab and quantitation of band intensity was done through Image J.
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bioRxiv - Synthetic Biology 2023Quote: ... both containing 0.1 % SDS and made from acrylamide / Bis-acrylamide solution (37.5:1, Biorad) according to established methods (69) ...
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bioRxiv - Genetics 2023Quote: ... Quantification of protein levels normalized to β-tubulin (12004165, Bio-Rad, 1:4000 dilution) was performed using Image J software.
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bioRxiv - Genetics 2024Quote: ... This solution was rapidly combined with 40 % acrylamide (37.5:1 mono:bis) solution (Bio-Rad) (sufficient for a final concentration of 2 %) ...
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bioRxiv - Molecular Biology 2023Quote: ... run in 1× TAE buffer and visualized with the ChemiDoc UV transilluminator (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... while HIF-1 was detected using high sensitivity Clarity Max Western ECL (Bio-Rad). For visualization of total protein content in supernatants ...
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bioRxiv - Developmental Biology 2024Quote: ... a ddPCR reaction mix containing 1× ddPCR Supermix for Probes (186-3023, Bio-Rad), 250 nM of probe ...
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bioRxiv - Cancer Biology 2024Quote: ... for 1 minute at RT and imaged on the ChemiDoc system (Bio-Rad, #17001401). Protein expression was normalized to housekeeping genes and quantified using ImageJ ...
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bioRxiv - Molecular Biology 2024Quote: ... This solution was rapidly combined with 40 % acrylamide (37.5:1 mono:bis) solution (Bio-Rad) (sufficient for a final concentration of 2 %) ...
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bioRxiv - Immunology 2024Quote: ... transferred to 1 ml columns with pre-wetted bottom frit (Bio-Rad, cat. 7326008) and further washed three times with 1 ml WB by gravity flow ...
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bioRxiv - Microbiology 2024Quote: ... 80 µL was mixed with 320 µL 1% agarose (Bio-Rad Certified Megabase Agarose) at 45 C ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 µL of a 1:3000 dilution of HRP- conjugated protein G (Bio-Rad) and 100 µL of HRP substrate buffer (20 ml NaCitrate pH 4.5 + 1 ml ABTS + 0.4 ml H2O2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... radiodurans genomic DNA was isolated in agar plugs using the Bio-Rad CHEF Genomic DNA Plug Kit (Bio-Rad, CAT#170-3592) with an adapted protocol44 ...
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bioRxiv - Cancer Biology 2021Quote: ... The signal was detected by a chemiluminescent western blot detection kit (Thermofischer) and the blots were developed and visualized under a ChemiDoc system (Amersham, Bio-Rad, USA). The primary antibodies used were Twist2 (GeneTex ...
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bioRxiv - Genetics 2021Quote: ... assessment of protein concentration using Pierce™ BCA Protein Assay Kit (Thermo cat#23225) and addition of 4x Laemmli Sample buffer (Bio-Rad). 40ng of each sample was loaded onto Mini-PROTEAN GTX Precast Protein Gel (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng RNA was used for cDNA synthesis for each sample using the iScript cDNA Synthesis kit (Bio-Rad, Cat. No. 1708890). A CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ddPCR was performed on a Bio-Rad QX200 Droplet Digital PCR System using ddPCR Supermix for Probes (No dUTP) kit (Bio-Rad, 1863024) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... the total protein concentration was determined by the Bradford method using the Bio-Rad Protein Assay Kit II (Bio-Rad, 5000 002) and serial dilutions of bovine serum albumin as a calibration standard (BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected using the EZ-ECL Chemiluminescence Detection Kit for HRP (Biological Industries) and ChemiDoc XRS+ Gel Documentation System (Bio-Rad Laboratories). Images were taken using the ImageLab™ software (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Neuroscience 2021Quote: ... 400 ng of complementary DNA (cDNA) was synthesized using the reverse transcriptase iScript complementary DNA synthesis kit (Bio-Rad, Hercules, CA; # 1708891), then diluted to a concentration of 2 ng/μL ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: 250 ng of total RNA of each sample was reversely transcribed and amplified using the TargetAmp-Nano Labeling Kit for Illumina Expression BeadChips (Biozym Scientific GmbH, Hessisch Oldendorf, Germany) on a Biorad MJ Thermal Cycler (Biorad, Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... CLN5 transcripts were measured from the control and CLN5i neurons RNA by quantitative polymerase chain reaction[13] using an iTaq Universal Probes one-step kit (Bio-Rad, 1725141) on a Roche 96-well thermocycler ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaned samples were solubilized in the previous buffer described above and then quantified with the RCDC Protein Assay kit (Biorad, Hercules, CA). Ten μg of protein from each sample were digested in-solution using both Lys-C and Trypsin ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific) and a T100™ thermal cycler (Bio-Rad). The expression of selected genes was determined by RT-PCR using the iQ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using the Bio-Rad CFX Manager software ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolated RNA (50 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) in the 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2019Quote: ... First strand synthesis and real time PCR were performed using iScript kit and iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA) on Light Cycler 480 (Roche ...
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bioRxiv - Genomics 2019Quote: Mammalian cells were embedded in gel plugs and High Molecular Weight DNA was purified as described in a commercial large DNA purification kit (BioRad #170-3592). Plugs were incubated with lysis buffer and proteinase K for four hours at 50°C ...
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bioRxiv - Immunology 2019Quote: ... One microgram of RNA was used to synthesize first strand cDNA using the iScript cDNA synthesis kit from Bio-Rad (Hercules, CA). The primers used for qPCR were synthesized by IDT (Coralville ...
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bioRxiv - Cancer Biology 2019Quote: One microgram of total RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc., Hercules, CA). The PCR reactions were performed with 0.2 µl of cDNA template in 25 µl of reaction mixture containing 12.5µl of iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2019Quote: ... Quantification of gene expression was performed by using the KAPA SYBR-FAST One-Step qRT-PCR kit and the CFX-Connect Real-time PCR detection system (Bio-Rad Laboratories) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and total RNAs were obtained according to the manufacturer’s protocol and then quantified and synthesized into cDNA using a iScript cDNA Synthesis Kit (Bio-Rad, Hercules, USA) for SALL4 expression or using a High-capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... we synthetized 100ng of cDNA from immunoprecipitated and isolated polyribosome-associated RNA (see above for detailed procedure) as well as non-precipitated RNA (input) using the iScript cDNA synthesis kit (Bio-Rad, USA). cDNA was then preamplified using TaqMan assay following kit instructions46 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were reverse transcribed from 500 ng of total RNA in a 20 µL volume using iScript cDNA synthesis kit (Bio-Rad Laboratories). Real-time qPCR was carried out on a C1000™ Thermal Cycler (Bio-Rad Laboratories) ...
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bioRxiv - Genomics 2019Quote: ... 1 μg of each RNA sample was converted into single-strand cDNA with random hexamers using an iScript™ Select cDNA Synthesis Kit (Bio-Rad). To validate fusion transcripts ...
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bioRxiv - Bioengineering 2021Quote: ... the homogenate was centrifuged at 16,000g for 5 min and 5 µl of the supernatant was used for protein measurement in a microplate format utilizing the Lowry DC protein assay kit and γ-globulin for generating standard curve (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... PCR was performed using the AceQ qPCR SYBR Green Master Mix kit (Vazyme, Nanjing, China) and the MyiQ2 Real-time PCR system (Bio-Rad, USA) with the following cycling conditions ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatants were transferred into a new set of tubes and protein concentration was determined using Bio-Rad DC™ protein assay kit (Bio-Rad). Western blot protocol is the same as described before ...
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bioRxiv - Cell Biology 2021Quote: ... 25 ng of cDNA template was mixed with the primers to final concentrations of 200 nM and mixed with 5 μl of iTaqTM Universal SYBR® Green Supermix kit (Bio-Rad). The reactions were done in triplicates with the following conditions ...
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bioRxiv - Biochemistry 2021Quote: ... Quantitative cDNA detection was performed via quantitative real-time PCR (RT-qPCR) with the same gene-specific primers using the iQ™ SYBR® Green Supermix Kit (BIORAD). Serially diluted genomic DNA was used as a quantification standard.
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bioRxiv - Biochemistry 2021Quote: ... For analysis of isolated RNA semi-quantitative reverse transcription–polymerase chain reaction (RT) PCR was performed with the iScript™ cDNA Synthesis Kit (BIORAD, Germany) according to the manufacturer’s instructions using 500ng of isolated RNA ...
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bioRxiv - Cell Biology 2021Quote: ... in accordance with the manufacturer’s protocol then performing qPCR using a Sangon Biotech microRNA qPCR kit with SYBR Green (B532461) in a Bio-Rad CFX96™ real-time PCR detection system (Bio-Rad) using the following thermocycling conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Q-PCR was performed on a Bio-Rad IQ5 real-time PCR detection system by using iTaq Universal SYBR Green One-Step RT-qPCR kit (Bio-Rad Laboratories) according to the manufacturer’s instructions ...