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Citations for Bio-Rad :
551 - 600 of 1009 citations for 5α CHOLESTAN 3 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: One microgram of total RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc., Hercules, CA). The PCR reactions were performed with 0.2 µl of cDNA template in 25 µl of reaction mixture containing 12.5µl of iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2019Quote: ... Quantification of gene expression was performed by using the KAPA SYBR-FAST One-Step qRT-PCR kit and the CFX-Connect Real-time PCR detection system (Bio-Rad Laboratories) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... One μg of total RNA was used to perform reverse transcriptase–polymerase chain reaction (RT-PCR) using iScript supermix (Biorad, Hercules, CA). The sequence of PCR primers used are ...
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bioRxiv - Microbiology 2021Quote: ... Gels were stained with ethidium bromide (0.5 mg mL-1) and photographed using the Quantity One electrophoresis analysis system (Bio-Rad, CA, USA). Gel images were digitally normalized to a single DNA marker to reduce gel-to-gel banding pattern variability ...
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bioRxiv - Plant Biology 2020Quote: ... Q-PCR was performed on a Bio-Rad IQ5 real-time PCR detection system by using iTaq Universal SYBR Green One-Step RT-qPCR kit (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram was reverse transcribed to generate first-strand complementary DNA (cDNA) using IScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA). qRT-PCR experiments were performed with 2.5 μl of a 1:20 dilution of first-strand cDNA and LightCycler 480 SYBR Green I Master mix ...
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bioRxiv - Molecular Biology 2021Quote: Plasma viremia was assayed using one-step reverse transcriptase real-time PCR [TaqMan assay] with an automated CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). qPCR primer sets were taken from previously published studies(16) ...
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bioRxiv - Microbiology 2020Quote: ... All gene transcripts were quantified by quantitative PCR using qScript™ One-Step qRT-PCR Kit (Quanta Biosciences, #95057-050) on CFX96 real-time PCR system (Bio-Rad). Primer sequences for qPCR were as follow ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.6 with HCl) for one hour and then divided for blotting on the basis of the prestained protein ladder (Bio-Rad, 1610373). Primary antibodies (see antibody table for vendor and dilutions ...
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bioRxiv - Cancer Biology 2019Quote: ... Isolated RNA (100 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) performed on the 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... and ampR was measured by quantitative Reverse Transcriptase PCR (qRT-PCR) using the iTaq Universal SYBR Green One-Step kit (Biorad, Hercules, CA) and a CFX96 Touch Real-Time PCR detection system (Bio- Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA in agarose gels was stained using ethidium bromide and visualized using ultraviolet light in a BioRad Chemi-doc system with digital camera and Quantity One imaging software (BioRad, Hercules, CA). All gel images were exported from Quantity One as .TIFF images ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3-HB and n-butanol were separated with 5 mM sulfuric acid as the mobile phase and one of two column conditions: (1) an Aminex HPX-87H or Fast Acids anion exchange columns (Bio-Rad Laboratories) at 35 or 55 °C and a flow rate of 0.6 ml min-1 or (2 ...
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bioRxiv - Developmental Biology 2019Quote: ... Signal intensity was normalized for three invariant histone bands (Ponceau S stain) and quantified using Quantity One software (Bio-Rad Laboratories Inc.).
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bioRxiv - Immunology 2019Quote: ... One μg of total RNA was used to perform reverse transcriptase–polymerase chain reaction (RT-PCR) using iScript supermix (Biorad, Hercules, CA). The sequence of PCR primers used are ...
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bioRxiv - Cell Biology 2021Quote: ... and 10-14h after egg laying and reverse transcribed using oligo-dT primers with a One-Step RT kit (Bio-Rad 1725140). The coding sequence for each gene encoding a gene of interest was amplified from a cDNA template using Phusion High Fidelity DNA polymerase (Thermo Fisher F-530XL) ...
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bioRxiv - Biochemistry 2021Quote: RT-qPCR was performed using the 2019-nCoV RUO Kit (Integrated DNA Technologies, #10006713) in combination with the iTaq™ Universal SYBR® Green One-Step Kit (BioRad). Each 20 μl reaction contained 10 μl 2X iTaq universal SYBR® Green reaction mix ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Microbiology 2023Quote: ... modified from previously published primers/probe (44)) was performed using the BioRad iTaq Universal Probes One-Step Kit (BioRad, Cat. No. 1725140). Thermocycling was performed on a BioRad CFX Connect Real-Time PCR Detection System (BioRad ...
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bioRxiv - Physiology 2023Quote: ... One hundred nanograms of RNA was used as input to synthesize cDNA using the iScript cDNA synthesis kit (BioRad, Hercules, CA, USA). cDNA templates were used for quantitative real-time PCR ...
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bioRxiv - Developmental Biology 2024Quote: ... Chemiluminescent signals were detected using Chemi-Lumi One (Nacalai Tesque, 07880-70) and imaged by the ChemiDoc™ Touch MP system (Bio-Rad). Band intensities were quantified using Image Lab software (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% (v/v) acetic acid) and imaged using the Molecular Imager Gel Doc XR System and the Quantity One Software Package (Bio-Rad, Germany), or they were used for immunodetection ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers/probe mixtures (all from Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: RNA was diluted in nuclease-free water and combined with the reagents from the One Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, USA) and eGFP primer/probe set with final primer concentrations of 900 nM and probe concentrations of 250 nM to measure transgene expression ...
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bioRxiv - Genetics 2019Quote: ... PCR products were separated and analyzed by 3% agarose gel electrophoresis (Bio-Rad) and visualized by ethidium bromide staining.
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bioRxiv - Cell Biology 2021Quote: ... Blocking solution consisted in 3% nonfat dry milk (Blotting-Grade Blocker, BIO-RAD Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... 3 μL cDNA template and 4 μL iQ SYBR green supermix (Bio-Rad) in a total reaction volume of 12 μL ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were separated on a 3-8% Criterion XT tris-acetate gel (Biorad) or 4-15% Criterion TGX gel according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... were resolved by 10% denaturing PAGE in Mini Protean 3 Cell (Bio-Rad). Separated RNA was then transferred to a nylon membrane (Hybond-XL ...
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bioRxiv - Evolutionary Biology 2019Quote: ... comprising 3 μl 2X SsoFast EvaGreen Supermix with low ROX (BioRad CA, USA), 150 nM of each primer pair (1.5 μl per pair) ...
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bioRxiv - Molecular Biology 2021Quote: ... with 3 uL Precision Plus Protein All Blue prestained standards (Bio-Rad, #1610373) loaded as a ladder ...
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bioRxiv - Genomics 2022Quote: ... and 3 μl of 2x iProof HF Master Mix (BioRad, Hercules, CA, USA). The 3 μl of 2x master mix was dispensed using a Mantis Liquid Handler (Formulatrix ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 µl DTT and 1000 µl Purezol (Biorad, USA, CA, Cat No: 7326890) were added to each falcon tube and kept on ice for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... on an Opticon 2 from MJ Research with Opticon Monitor 3 software (BioRad). Reactions were set up manually using an 8-channel pipette (30-300μL ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... or 3-8% Tris-Acetate CriterionTM XT Precast Gels (Bio-Rad Laboratories, Invitrogen) at 150 V for 90 min ...
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bioRxiv - Microbiology 2020Quote: ... using the qScript XLT One-Step RT-qPCR Tough Mix (Quanta BioSsciences, Beverly, MA, USA) on the CFX96 Real-Time thermocycler (BioRad, Hercules, CA, USA) using a 20-minute reverse transcription step and 45 cycle qPCR in a 20 µl reaction volume ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed on 96-well optical reaction plates with one-step SYBR Green PCR master mix (Bio-Rad Laboratories, Cat#1725150).
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bioRxiv - Biochemistry 2021Quote: ... The gel images were obtained using an Amersham™ Typhoon™ scanner (Cytiva) and were quantified using Quantity One (BIO-RAD, version 4.6.9) or ImageJ ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µg of each protein sample was separated in one dimension using 4-20% tris-glycine gels (Mini-PROTEAN TGX, Bio-Rad Laboratories, Inc.). A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl ...
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bioRxiv - Plant Biology 2020Quote: ... and the quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the iScript™ One-Step RT-PCR Kit (Bio-Rad®), marking with SYBR® Green (Bio-Rad®) ...
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bioRxiv - Microbiology 2020Quote: ... MNV or HAV RNA recovered by the ISO 15216-1 and PGM-MB or HAV antibody methods was quantified using the One-Step dd-RT-PCR Advanced Kit for Probes (Bio-Rad Laboratories, Ltd.) according to manufacturer’s instructions and as described previously (23 ...
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bioRxiv - Microbiology 2020Quote: ... using Script XLT One-Step RT-qPCR Tough Mix (Quanta Biosciences, Beverly, MA, USA) on a CFX96 Real-time thermocycler (BioRad, Hercules, CA, USA). Plate controls included a quantitated SARS-CoV-2 N-specific qPCR positive control ...
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bioRxiv - Microbiology 2020Quote: ... was used for quantification and all PCR reactions were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad Cat# 1864022). Primers used were previously described in (25) ...
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bioRxiv - Microbiology 2021Quote: ... The quality of extracted DNA samples was also checked by loading one μl of DNA in 1% Agarose Gel (Bio-Rad, California, USA). The extracts were stored at -20 C until use.
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed by using a HiScript II one-step qRT-PCR SYBR green kit (Vazyme) on a CFX96 real-time PCR detection system (Bio-Rad Laboratories, Inc.). The following primers were used ...
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bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α was analyzed by using quantitative real-time PCR according to the Biorad iScript One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers (F/R) ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and PCR amplification were performed in a single reaction using the One-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, CA, USA) per the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: One hundred nanograms of total RNA was reverse-transcribed (RT) and amplified using the iScript One-Step RT-PCR kit for probes (Bio-Rad, Hercules, CA). Real-time qPCR was performed with the Bio-Rad CFX96 sequence detection system using predesigned primer/probe sets against CPT1a ...
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bioRxiv - Molecular Biology 2023Quote: ... and optic density of the bands corresponding to mouse PRM1 and PRM2 was quantified using Quantity One 1-D analysis software (BioRad, Hercules, CA, USA) to calculate the PRM1/PRM2 ratio ...