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Citations for Bio-Rad :
451 - 500 of 1009 citations for 5α CHOLESTAN 3 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Detection of viral RNA using one-step real-time reversetranscription PCR was performed according to (Corman et al., 2020) with iTaq Universal Probes One-Step Kit (Biorad) using primers and probes against the N-gene (N_Sarbeco_F ...
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bioRxiv - Biochemistry 2020Quote: ... The intensities of NAGS-M bands were measured using a GS-800 Calibrated Densitometer and Quantity One software (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... The radioactive signals were analyzed using a Bio-Rad phosphorimager and the Quantity One image-analysis software package (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... Visualisation and quantification were carried out using a Personal Molecular Imager with Quantity One 1-D analysis software (Bio-Rad) and data were analyzed with GraphPad Prism 4 software.
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bioRxiv - Microbiology 2021Quote: ... and the image was registered with the help of a Chemidoc–XRS image analysis device and Quantity One–SW software (BioRad).
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bioRxiv - Microbiology 2020Quote: cDNA synthesis and quantitative reverse transcription PCR (qRT-PCR) were performed using the iTaq Universal SYBR Green One-Step Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... qPCR was performed using the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad, Cat No.: 172-5150) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Viral genomes were quantified by Taqman one-step qRT-PCR on a CFX96 Touch real-time PCR detection system (BioRad) and were reported on a log10 scale measured against standard curves from either a ZIKV A-plasmid as previously described (69) ...
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bioRxiv - Microbiology 2022Quote: cDNA was prepared for RT-qPCR by reverse transcribing one microgram of the extracted RNA using iScript Reverse Transcription Supermix (BioRad). qPCR was performed using iTaq-Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Microbiology 2022Quote: HCV RNA copies were determined by Taqman RT-qPCR using a Quanta BioSciences qScript XLT One-Step RT-qPCR kit according to the manufacture’s instruction (Bio-Rad). GLT1_probe was used to detect for GLT1 RNA ...
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bioRxiv - Immunology 2021Quote: ... The gel image was acquired in the Gel Doc 1000 system and analyzed using the Quantity One software (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... the gels were either stained with One-Protein Blue gel stain (#21003-1L, Bioticum) or transferred onto PVDF membranes for immunodetection using TurboBlot system (Biorad).
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bioRxiv - Evolutionary Biology 2020Quote: ... or ∼50 ng (8-14 months post-hatch) of total RNA as template in one-step reverse transcription droplet digital PCR (RT-ddPCR) [66] (BioRad) with TaqMan probes specific to vgll3 alleles to quantify vgll3 absolute expression (Supplementary table 1) ...
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bioRxiv - Immunology 2019Quote: ... Proteins were separated at 200V for approximately one hour and transferred onto either PVDF or nitrocellulose membrane using the TransBlot Turbo Transfer (BioRad) system ...
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bioRxiv - Developmental Biology 2020Quote: ... The protein band of interest was subsequently quantitated (normalized to the reference protein band) using quantity one software (Bio-rad). Antibodies used for western blot analysis are listed in Suppl ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (12.5 ng) was reverse transcribed and amplified with the iTaq Universal SYBR Green One-Step Kit (Bio-Rad). RUNX1 and ACTB were amplified in parallel in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... 12.5-50 μg of protein was mixed with one third volume of 4X Laemelli Sample Buffer (Bio-Rad Laboratories 1610747) and boiled at 100 °C before loading into a polyacrylamide gel alongside either the PageRuler™ or PageRuler™ Plus Prestained Protein Ladder ...
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bioRxiv - Biochemistry 2020Quote: ... 40S and 80S fractions were quantified using Quantity One 1-D Analysis Software v.4.1.2 (Bio-Rad Laboratories, Hercules, CA). Pno1 was quantified using ImageJ Software v1.52 (100 ...
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bioRxiv - Cell Biology 2021Quote: ... Gene expression analysis was conducted with 50 ng RNA using iTaq Universal One-Step RT-qPCR Kit (Bio-rad, 1725140). The reaction was carried out according to the manufacturer’s instructions using CFX Connect Real-Time PCR Detection System (Bio-rad ...
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bioRxiv - Microbiology 2019Quote: ... ZIKV RNA levels were determined by TaqMan one-step quantitative reverse transcription PCR (qRT-PCR) on a CFX96 Touch Real-Time PCR Detection System (BioRad) using standard cycling conditions ...
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bioRxiv - Cell Biology 2021Quote: ... The immunodetected protein bands were then analyzed using ChemiDoc XRS system with Quantity One software (Bio-Rad, Richmond, CA, USA).
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bioRxiv - Genomics 2021Quote: ... 20 μl ddPCR mix consisted of 5μl One-Step RT-ddPCR Advanced Supermix for Probes (Bio-Rad, Laboratories, Munich, Germany), 2 μl of Reverse Transcriptase (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and cDNA amplification were done using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, Hercules, CA) and a CFX96 real-time system (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: 1 μg of total RNA purified from differentiated cells was reversed transcribed using the One-step SuperScript kit (Bio-Rad). The qRT-PCR reaction was performed in a CFX96 Real-time PCR detection system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... One half microgram of total RNA was reverse transcribed into cDNA using an iScript cDNA Synthesis kit (Bio-Rad, 1708891). All TaqMan primers and probes were obtained from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... One µg of total RNA was used for first-strand cDNA synthesis with the iScript cDNA Synthesis Kit (Bio-Rad) and diluted 8 times afterwards ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for one h at room temperature (RT) with horseradish peroxidase (HRP)-coupled secondary Abs at 1:2000 for one h (all secondary Abs from Biorad, raised in goatanti-rat HRP Cat # 5204-2504 and anti-mouse HRP Cat # 1721011) ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA levels were determined by TaqMan one-step qRT-PCR on a CFX96 Touch Real-Time PCR Detection System (BioRad) using standard cycling conditions ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 6-week-old rosette leaves and bolting flower buds of Col-0 and one of the RPF2-atp1 (RPF2-atp1-9) transformed lines (T3) with PureZol reagent (BioRad). Three independent libraries for each genotype were made from total RNA treated with 250 ng of Turbo DNase (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... The following qRT-PCR thermocycling protocol was used with the iTaqTM universal SYBR® Green One-Step kit (Bio-Rad): 95 °C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... The reaction mixture was separated on SDS-PAGE and the phosphorylated receptor was autoradiographed for image analysis with the Quantity One program (BioRad) on phosphor screens.
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bioRxiv - Cancer Biology 2022Quote: ... A total of one μg of total RNA was used for reverse transcription with iScript cDNA synthesis kit (Bio-Rad). Real-time PCR was performed using SYBR Green PCR Master Mix (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... One μg of RNA was used to perform the reverse transcriptase reaction with iScript TM cDNA Synthesis Kit (Bio-Rad). Changes in mRNA levels of the target genes were determined by relative RT-qPCR with a CFX96TM Real‒Time PCR Detection System (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... 200 ng of purified RNA was immediately reverse transcribed at 37 °C for one hour in an S1000 Thermal Cycler (BioRad) via the OmniScript RT Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time RT-qPCR was performed using the AB StepOnePlus Detection System and the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Transcripts were measured by TaqMan one-step qRT-PCR on a CFX96 Touch real-time PCR detection system (Bio-Rad) and were reported as relative differences in threshold cycle (Ct ...
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bioRxiv - Molecular Biology 2024Quote: ... and 100ng of RNA was used with iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad, CA, USA) to perform a one-step RT-qPCR in a volume of 20 μl using a QuantStudio3 real-time PCR instrument from ThermoFisher (Waltham ...
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bioRxiv - Bioengineering 2024Quote: ... The proteins were incubated for one hour at 65 and 70°C in a thermocycler with heated lid (T100, BioRad) Binding to D25 and MPE8 antibodies was measured afterwards following the protocol described above.
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bioRxiv - Cancer Biology 2024Quote: ... Conversion of one µg of RNA into cDNA was performed using the iScript cDNA Synthesis Kit (1708890; Bio-Rad Laboratories) and Master Cycler Pro Device (EPPE6324000.516 ...
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bioRxiv - Biophysics 2020Quote: ... 3 mL of acrylamide/bis solutions (40%, Bio-Rad Laboratories, CA, USA), 1 mL of 10X tris-borate-EDTA (TBE ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of proteins were mixed !3-mercaptoethanol-supplemented Laemmli buffer (BioRad), heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (4 –15% Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 trans-blot papers (Bio-Dot SF Filter Paper, Bio-Rad, #1620161) and one cellulose acetate membrane (Cellulose Acetate Membrane Filters ...
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bioRxiv - Neuroscience 2021Quote: ... All Blue Prestained Protein Standards (1-3 µl; BioRad Laboratories, Hercules, CA) were used to identify band molecular weights ...
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bioRxiv - Bioengineering 2022Quote: ... along with 3 μL of Precision Plus protein ladder (Bio-Rad #1610374), and gels were run at 125 V for 1.25 hours in Tris-Glycine running buffer (ThermoFisher #LC2675).
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 3 times with 0.1% Tween-20 (Bio-Rad,1706531) TBS (TBTS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... were loaded onto 3-8% Criterion XT tris-acetate gels (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and anti-F4/80 FITC (3:100, Bio-Rad Laboratories, Temse, Belgium). The single cells were washed with 1XPBS+1%FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and then washed again 3 times before revelation by electrochemoluminescence (Bio-Rad) using the Chemi-doc XRS+ (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... After 3 washes with 0.05% Tween 20 (catalog number 1706531, Bio-Rad) in PBS (TPBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... to 3 mL for buffer exchange with an Econo10 DG column (Biorad) equilibrated in buffer A and eluted with 4 mL of the same buffer ...