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Citations for Bio-Rad :
5801 - 5850 of 9194 citations for Glutathione Fluorescent 384 Well Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... This cDNA was then run on a 2% agarose gel in TAE buffer alongside a 100bp-1kb DNA ladder (Bio-Rad). After electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 µg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then transferred to a PVDF membrane at 400mA for 2 hours at 4°C in 1x Tris/Glycine buffer (1610771, Bio-Rad). Membranes were then blocked in casein blocking buffer (PI37528 ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... before solubilisation in 2% SDS (Severn Biotech, Worcs, UK) and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad). Data are expressed as OD595 of selected timepoint relative to OD595 at day 0.
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bioRxiv - Cancer Biology 2023Quote: ... and the fold change was calculated using the 2−ΔΔCT method using Bio-Rad CFX384 Touch Real-Time PCR System and software (Bio-Rad). The results were expressed either as the relative log2 FC (fold change ...
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bioRxiv - Biophysics 2023Quote: ... The subsequent mixture was gently rotated at RT for 20 min prior to the addition of 250 mg SM-2 Bio-Beads (Bio-Rad) per 5 ml of sample ...
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bioRxiv - Cell Biology 2023Quote: ... The total cDNA obtained was diluted 1:10 and 2 µl was used for qRT-PCR by using gene specific primer and SYBR green PCR mix (BIO-RAD) on BIO-RAD CFX96 real time PCR machine ...
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bioRxiv - Microbiology 2023Quote: ... 2% formaldehyde gel and separated at 125-150V at 4°C for 1-2 h and then transferred to a Zeta-Probe GT membrane (Bio-Rad) via capillary action overnight (Streit et al. ...
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bioRxiv - Immunology 2023Quote: ... Labeled glycans were dissolved in HPLC grade water (FisherScientific) and excess unbound APTS removed using Agencourt CleanSEQ beads (non-antigen specific glycans) and Bio-Gel P-2 (Bio-rad) size exclusion resin (RBD-specific glycans) ...
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bioRxiv - Neuroscience 2023Quote: ... F0 fish were screened by high resolution melt (HRM) analysis using gene-specific primers (Table 2) with Precision melt supermix (Biorad 1725112) according to the manufacturer’s protocol in a BioRad CFX RT-PCR thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR products were run on a 2% agarose gel and images were taken on a Chemidoc MP imaging system (BIO-RAD). Image J was used to analyze the pixel intensity of 3R (288 bp ...
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bioRxiv - Biochemistry 2023Quote: ... for 2 h (for the ERGIC-53 ΔΗ34 mutant) The beads were transferred into a gravity flow column (Econo-column, Bio-Rad) and washed with 20 CV of buffer A supplemented with 0.02% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and transfers of proteins to .45uM nitrocellulose membranes were performed using the Protean 2 or 3 systems (Bio-Rad) and protocols provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... was used for positive signals and the chemiluminescence was detected using a Light Capture AE-6971/2 device (ATTO) or ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... before being re-incubated with the secondary antibodies at room temperatures for 2 h and then subjected to the imaging of immunoblots by Bio-Rad instrument ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Biochemistry 2022Quote: An equivalent volume of ∼150 µg FBS was first diluted 1:2 with loading buffer (consisting of 2X Laemmli Sample Buffer (#1610737, Bio-Rad) plus a 5% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription reaction was conducted using 0.5 µg of total RNA and 2 µl of iScript™ Reverse Transcription Supermix (Bio-Rad) in 10 µl reaction volume ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747, BioRad, Hercules, CA, USA) and run on a Mini-PROTEAN® TGX™ Precast Gel (#4561083 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by washes at room temperature (1 x 15 min, 2 x 5 min) and incubation with HRP-conjugated secondary antibodies (Bio-Rad) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... before solubilisation in 2% SDS (Severn Biotech, Worcs, UK) and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad). Data were normalised by dividing raw OCR/ECAR measurement by OD595 for each well of the culture plate ...
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bioRxiv - Biochemistry 2023Quote: ... This mixture was then incubated at 4°C for 2 h with gentle agitation before reconstitution was initiated by removing detergent by incubating with Bio-beads (Bio-Rad) at 4°C overnight with constant rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction products were visualized on a 2 % agarose gel with 0.05% (v/v) Ethidium Bromide and visualized with a Gel Doc XR+ imager (Bio-Rad Laboratories). PCR amplicons were column purified with the Monarch® PCR & DNA Cleanup Kit and eluted in 25 µL of Monarch® DNA Elution Buffer.
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bioRxiv - Biophysics 2024Quote: ... then diluted below the CMC of C12E8 with reconstitution buffer and added to 0.5 g methanol-activated Biobeads SM-2 (Bio-Rad; 1523920). The mixture was rotated end over end for 18 hours and then subject to room temperature size exclusion chromatography on a Superose 6 10/300 GL column ...
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bioRxiv - Neuroscience 2024Quote: ... Viability of magnetically-captured cells (eluted into a new tube away from the magnet) was assessed by trypan blue staining (1:2 dilution sample in trypan blue; BioRad 1450013). Cell viability was quantified as the proportion of cells that did not take up the dye ...
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bioRxiv - Genetics 2024Quote: ... Membranes were were in contact with the luminol-H2O2 solution for 2 minutes and then exposed for imaging using the Chemidoc imaging system (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... clarified at 20,000 g for 2 minutes and 15 µL was loaded onto a 4-20% tris-glycine SDS-PAGE gel (Biorad #5671094). Proteins were then transferred to nitrocellulose (Sigma #10600001 ...
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bioRxiv - Microbiology 2024Quote: ... 10 µg Capped mRNA transcripts were electroporated into BHK-21 cells in a 0.2 cm cuvette (25 μF, 1.5 kV, and 200 Ω) with the help of Gene Pulser Xcell Electroporation system (Bio-Rad) and resuspended in antibiotic-free DMEM supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2020Quote: ... Total protein concentration was determined with Bradford protein assay kit (Bio-Rad). An equal amount of protein was loaded onto 12% SDS-PAGE gel for western blot ...
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bioRxiv - Biochemistry 2021Quote: Reverse transcription was performed using the iScript cDNA Synthesis Kit (Bio-Rad) following the manufacturer’s instructions with 10-fold diluted DNase-treated RNA ...
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bioRxiv - Biochemistry 2021Quote: Reverse transcription was performed using the iScript cDNA Synthesis Kit (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed with the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... First strand cDNA was generated with an iScript cDNA synthesis kit (BioRad). Real-time PCR was performed in triplicate ...
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bioRxiv - Cell Biology 2020Quote: ... and iScript cDNA Synthesis Kit (Cat. #: 1708891) were purchased from Bio-Rad. Tissue Culture Treated 24-well plate (LPS Cat ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was reverse transcribed using the iScript cDNA Synthesis kit (Bio-Rad) and qPCR was performed on a Bio-Rad C1000 Touch thermal cycler using the resultant cDNA ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were measured using a DC Protein Assay kit (Bio-Rad) with BSA protein standards and AccuSkan GO spectrophotometer (Fisher Scientific) ...
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High resolution, serial imaging of early mouse and human liver bud morphogenesis in three dimensionsbioRxiv - Developmental Biology 2021Quote: ... and iScript cDNA Synthesis Kit (Cat. #: 1708891) were purchased from Bio-Rad. Tissue Culture Treated 24-well plate (LPS Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized using the iScript Advanced cDNA Synthesis kit (Bio-Rad). qPCR was performed using 5ng cDNA with the SsoAdvanced Universal SYBR Green Mix (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was converted to cDNA using the iScript reverse transcriptase kit (BioRad) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized using the iScript Advanced cDNA Synthesis kit (Bio-Rad). qPCR was performed using 5ng cDNA with the SsoAdvanced Universal SYBR Green Mix (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was reverse transcribed using the iScript cDNA Synthesis kit (BioRad 1708890). apob-1 and apob-2 levels were detected using the Fast Start Essential Green DNA master mix (Roche 06924204001 ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration was determined using the Bio-Rad kit (BioRad Laboratories, USA).
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bioRxiv - Developmental Biology 2021Quote: ... Mitochondrial protein concentration was determined using the Dc-protein determination kit (BioRad).
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bioRxiv - Immunology 2022Quote: ... RNA was then reverse transcribed using the iScript cDNA synthesis kit (BioRad), as per the manufacturer’s instructions ...