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Citations for Bio-Rad :
5751 - 5800 of 9194 citations for Glutathione Fluorescent 384 Well Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... detergent was removed from the sample by the gradual addition of 400 mg/mL SM-2 polystyrene Bio-Beads (Bio-Rad) over the course of four hours ...
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bioRxiv - Genetics 2022Quote: ... The entire sample was loaded on 1.5 mm 8% SDS-PAGE gel and ran at 85 volts for 2 hours and transferred to PDVF membrane via semidry transfer (Bio-Rad) at 19 V for 2 hours and 30 minutes ...
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bioRxiv - Genetics 2022Quote: ... The samples were boiled at 65°C for 10 minutes and then loaded on 10% SDS-PAGE gel and ran at 90 volts for 2 hours and transferred to a PDVF membrane via semidry transfer (Bio-Rad) at 13 V for 3 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 0.2 μm nitrocellulose membranes were prewet for 10 minutes in TBS then secured into the Bio-Dot Microfiltration apparatus (Bio-Rad). Wells were rehydrated once with TBS ...
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bioRxiv - Neuroscience 2022Quote: ... was performed for 2 h at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD).
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bioRxiv - Cell Biology 2022Quote: ... The PCR product was separated in a 2% agarose gel and imaged by ChemiDoc™ Touch Imaging System (Bio-rad Laboratories).
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with appropriate primary and secondary antibodies listed in Table 2 in 5% milk and developed using the Clarity Western enhanced chemiluminescence substrate system (1705060, Bio-Rad) in a Xograph Compact X5 processor ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad) were added and incubated for 16 hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad) were added and incubated for 16 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2021Quote: ... Primers (Supplementary Table 2) were designed using Primer3 Plus (24, 25) to quantify transcripts using Universal SYBR Green Supermix (Bio-Rad) using a Quantstudio 6 Flex (Applied Biosystems ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The RNA sample was denatured by incubating it at 70°C for 2 min in S1000TM or C1000TM Thermal Cycler PCR machine (Bio-Rad). The denatured RNA was quickly transferred on ice ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was diluted 1:4 in nuclease free water and each cDNA was combined with segment specific primers (Supplementary Table 2) and Precision Melt Supermix (Bio-Rad) and analyzed by qPCR in a CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation for 2 hours at room temperature with HRP-conjugated goat anti-rabbit or goat anti-mouse secondary antibodies (Bio-Rad), diluted 1:5000 in 5% nonfat milk in TBS-T ...
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bioRxiv - Synthetic Biology 2022Quote: ... Reaction mixtures were incubated for 2 hours at 25 °C and quenched by adding 10 μL 4x Laemmli Sample buffer (Bio-Rad). Samples were boiled at 95 °C for 5 min and each 8 μL were loaded onto 12% SDS-PAGE gels ...
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bioRxiv - Plant Biology 2022Quote: ... 500 nM concentration of each PCR primer and 2 μL of 15x diluted cDNA templates using CFX96 real-time PCR system (Bio-Rad) as described (Gayomba et al. ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The reactions were assembled isothermally on a PTC-225 Peltier Thermal Cycler (MJ Research) or a Tetrad 2 Peltier thermal cycler (Bio-Rad) at a suitable growth temperature (e.g ...
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bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions or ISVPs (2×1012 particles/ml) were incubated for 5 min at the indicated temperatures in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Zoology 2020Quote: ... Samples were incubated in a 1:1 (w/v) ratio of 2× sample buffer (0.2M DTT, BioRad Laemmli Sample Buffer #1610737) at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of each protein sample was denatured and reduced by boiling at 95°C for 5 min in 10% (v/v) 2-mercaptoethanol-containing Laemmli sample buffer (Bio-Rad). Samples were resolved by SDS-PAGE with a precast Tris-glycine gel (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: ... to nitrocellulose membranes using a constant voltage of 100 V for 2 h using the BioRad Midi PROTEAN Western blotting module according to the manufacturer’s instructions (BioRad, CA, USA). The membranes were blocked with blocking solution [Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads were washed five times with IP lysis buffer and were boiled for 5-10 min in 2 times Laemmli sample buffer (#1610737, Bio-Rad) with 2-mercaptoethanol (#BP176-100 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% (w/v) SDS and 6% (v/v) β-mercaptoethanol) and protein concentrations were quantified using Bradford Protein Assay (Bio-Rad). Equal concentrations of protein were fractionated on NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Biochemistry 2021Quote: ... was incubated at 55°C for 2 h and then purified by 6% non-denaturing PAGE using a Prep Cell apparatus (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... The mixture was transformed into Escherichia coli SS320 cells by electroporation in a 2-mm cuvette using a Gene Pulser Xcell (Bio-Rad) with a setting of 2,400 volts ...
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bioRxiv - Biochemistry 2021Quote: ... Proteoliposomes or protein-free liposomes were generated by two rounds of treatment with 300 mg of wet SM-2 Bio-Beads (Bio-Rad) in total ...
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bioRxiv - Neuroscience 2021Quote: ... In a water bath samples were boiled for 2 minutes and 12 ul of each lysate were loaded on two separate gels (Biorad, #4568096), one each required for parallel HTT and pS13 HTT detection ...
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bioRxiv - Microbiology 2020Quote: ... Optimized ddPCR was used to detect the presence of SARS-CoV-2 viruses following our previous study.10 Analysis of the ddPCR data was performed with QuantaSoft software (Bio-Rad). The concentration reported by the procedure equals copies of template per microliter of the final 1x ddPCR reaction ...
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bioRxiv - Immunology 2020Quote: ... in reducing or nonreducing conditions using SuperSep Ace 5%–20% gradient polyacrylamide gel (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 2 × Laemmli Sample Buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... the primer pairs (Table 2; exception: S. umbla) were used to create EvaGreen-based droplet dPCR assays using the AutoDG (Bio-Rad) for droplet generation ...
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... The Ct value was determined by CFX Maestro software when the fluorescence rises exponentially 2-fold above background (Biorad, Hercules, CA.). The reference group was the negative control birds.
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bioRxiv - Neuroscience 2021Quote: ... Gels were run for 2 hours and 10 min at 80V and transferred using the Trans-Blot SD Semi-Dry Transfer cell system (Bio-Rad). After transfer the LI-COR Revert 700 Total Protein Stain (LI-COR ...
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bioRxiv - Molecular Biology 2020Quote: ... or in transfer buffer (25 mM Tris, 200 mM glycine, 20% ethanol) for 2 h at 52V with a liquid transfer system (Bio-Rad). Total proteins were revealed by Pierce® Reversible Stain (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... and by linear regression in log10 copies/ml with a standard curve realized from a SARS-CoV-2 positive nasopharyngeal sample quantified by Droplet-Digital PCR (Bio-Rad). IC50s were analyzed by nonlinear regression using a four-parameter dosage-response variable slope model with the GraphPad Prism 8.0.2 software (GraphPad Software ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins (10μl solubilized in 2X Laemmli/20% of 2-mercaptoethanol) were separated by SDS-PAGE on 10% polyacrylamide gel (Bio-Rad, Hercules). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The mixture was transferred to a pre-chilled 2 mm gap electroporator cuvette and cells were electroporated using the EC2 program (MicroPulser, Bio-Rad). Cells were then suspended in 3 ml 1/4 TSB (at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Three more washing steps of 2% w/v TBST/milk were applied and finally the membranes were screened with a ChemiDoc MP Imaging system (Biorad, USA) and freshly mixed ECL fluorescent mixture and optimal exposure times ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Neuroscience 2023Quote: ... The pellets were resuspended with 2 % SDS and loaded onto a cellulose acetate membrane assembled in a slot blot apparatus (Bio-Rad). The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... tabacum) BY-2 suspension-cultured cells and biolistic bombardment using a PDS-1000/He biolistic particle delivery system (Bio-Rad Laboratories) were performed as previously described (Banjoko and Trelease ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral copy numbers were determined using SARS-CoV-2 RdRp gene specific primers and probe (see [28]) and droplet digital PCR (Bio-Rad Laboratories Inc. ...
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bioRxiv - Immunology 2022Quote: Serum samples were obtained from blood collected from the submandibular vein at the indicated timepoints and incubated on Kallestad HEp-2 slides (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were prepared for loading by mixing 30 μg of protein in 5xSDS sample buffer containing 15% 2-Mercaptoethanol (Bio-Rad) and heated at 90 °C for 5 minutes ...
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bioRxiv - Physiology 2022Quote: ... followed by a final melting curve analysis from 70°C to 95°C with 0.2°C increment for each step using the CFX96 Real-Time PCR System and the Precision Melt Analysis software (Bio-Rad). For HMA analysis ...
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bioRxiv - Genomics 2024Quote: ... Samples were then run on a 2% agarose gel with 1x SYBR Safe for 2 hours at 120 V before gel extraction with a Freeze ‘N Squeeze column (Bio-Rad). After extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% of total input and 15% of total IP fraction were resolved on 10% SDS-PAGE gels and wet transferred onto a 0.45 µm nitrocellulose membrane for 2 h at 80 V and 4°C (Bio-Rad, Germany). The membrane was blocked for 1 h in 5% BSA in PBST (PBS + 0.2% Tween20) ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR was performed with the 2× S6 SYBR Premix EsTaq plus (Scintol) on the CFX Connect Real-Time System (Bio-Rad). The data were analysed using the 2-ΔΔCt method ...
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bioRxiv - Neuroscience 2023Quote: ... and the supernatants were incubated with either 2 µg of anti-PAR antibody (clone #19, custom designed at AbD Serotec, Bio-Rad) or Human IgG F(ab)2 (Rockland ...