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Citations for Bio-Rad :
5701 - 5750 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The cDNA used for ddPCR was transcribed using the iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Biorad, Munich, Germany).
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 µm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using iScriptTM cDNA synthesis kit and qRT-PCR was performed using a CFX96 Touch Real-Time PCR Detection System (BioRad). Data were analyzed using the following equations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA (1μg) was reverse transcribed into complementary DNA using an iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... TX) and 750ng of RNA was reverse transcribed to generate cDNAs using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Relative transcript levels were analyzed by real-time qPCR using SYBR Green (SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified with a dsDNA HS Assay kit on a Qubit fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... aliquots constituting 250 ng of RNA were diluted with nuclease-free water to a volume of 15 µL and processed using the iScriptTM cDNA Synthesis Kit (BioRad Laboratories Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was measured using the modified Lowry assay (DCTM Protein Assay Kit, BIO-RAD, 5000111). The same amount of total protein was mixed with Laemmli sample buffer (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... 500 ng RNA was reverse transcribed using HiScript III 1st Strand cDNA Synthesis Kit followed by qPCR reactions using SYBR Green master mix and analyzed by Biorad CFX Touch Real-Time PCR Detection System (SIA-PCR008) ...
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bioRxiv - Biophysics 2023Quote: ... proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Membrane strips were finally washed 6x for 3mins in 1X TBST before being developed with the ECL Clarity Kit (BioRad) and imaged on ChemiDoc Imaging system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... High integrity RNA (RIN > 7) was used to synthesize cDNA with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). PCR primers for human B-ACTIN ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufacturer’s instruction and measured using the Eon High Performance Microplate Spectrophotometer (BioTek ...
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bioRxiv - Microbiology 2022Quote: The secretome samples were fractionated by one-dimensional SDS-PAGE with the Mini-protean® 3 kit (Bio-Rad, USA) using a 12% resolving gel and a 5% stacking gel at 200 V for 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and cDNA amplification were done using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, Hercules, CA) and a CFX96 real-time system (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... according to manufacturer’s instructions and 200 ng RNA was used for cDNA synthesis using the iScript cDNA Synthesis kit (Bio-Rad). Quantitative real-time PCR was performed in technical triplicates using Power SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... viable cells were enriched using a dead cell removal magnetic kit (130-090-101, Miltenyi Biotech) and cellular viability was assessed using Trypan blue staining (0.4%; 1450013, Bio-Rad) and an automated cell counter (TC20 ...
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bioRxiv - Cell Biology 2022Quote: ... and the resulting supernatants were normalized by total protein concentration measured by the RC DC Protein Assay kit (Bio-Rad). Equal volumes of retinal lysate containing 10 μg total protein from each mouse genotype were subjected to SDS/PAGE ...
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bioRxiv - Bioengineering 2022Quote: ... droplet-digital PCR was performed for 16 extracted RNA samples using the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad) and the CFX96 thermocycler (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... One half microgram of total RNA was reverse transcribed into cDNA using an iScript cDNA Synthesis kit (Bio-Rad, 1708891). All TaqMan primers and probes were obtained from Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The agarose gel plugs were prepared and processed with the CHEF Mammalian Genomic DNA Plug Kit (BioRad, Cat. No. #1703591). Total RNAs used to construct RNA-seq libraries were extracted from various tissues of a female juvenile zebra shark (total length ...
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bioRxiv - Plant Biology 2022Quote: ... One µg of total RNA was used for first-strand cDNA synthesis with the iScript cDNA Synthesis Kit (Bio-Rad) and diluted 8 times afterwards ...
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bioRxiv - Microbiology 2022Quote: ... HRP-linked Goat αRat antibody (Pierce 31470) was used at 1:30,000 and detected with an HRP Substrate kit (Bio-Rad) and Bio-Rad ChemiDoc imager ...
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bioRxiv - Microbiology 2022Quote: ... in 1x TBS buffer for 30 minutes at 250 V and transferred using the Trans-Blot turbo Mini 0.2 μm PDVF transfer kit (Bio-Rad) with a Transblot Turbo transfer system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 50 μL of 1X PBS and protein concentration was using The DC Protein Assay Kit (Biorad).
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bioRxiv - Microbiology 2022Quote: ... on cDNA samples using the LightCycler 480 SYBR Green I Master kit in the CFX384 RT-PCR Detection System (Biorad). The identities of the resulting amplicons were checked by melting-curve analysis using the CFX384 software ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng DNase-treated RNA was used as template for cDNA synthesis using the iScript cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad). The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... The cDNA utilized in the PCR experiments was generated via the iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules California #1708890). PCR Reactions were then performed in triplicate (3n ...
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bioRxiv - Neuroscience 2023Quote: ... a cDNA library was generated using 500 ng of total RNA using the iScript cDNA synthesis kit from Bio-Rad. The concentration of the cDNA was then quantified using the Qubit 2.0 assay for oligos per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were hybridised with an appropriate DNA probe— as indicated in figure legends and Table S2—radiolabelled with P32 prepared via random priming using the High Prime kit (BioRad). Pre-hybridisation solution was discarded ...
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bioRxiv - Physiology 2023Quote: ... was reverse transcribed and converted to complementary DNA (cDNA) using the iScript cDNA synthesis kit (1708891, Bio-Rad, Hercules, CA). Each cDNA sample was amplified with messenger RNA (mRNA ...
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bioRxiv - Molecular Biology 2022Quote: 500-1000 ng of RNA were used for the retrotranscription reaction using iScript cDNA Synthesis Kit (BioRad, CA, USA, #1708890). Expression values were determined by q-PCR using Sybr Green (iTaq SYBR Green Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein concentrations of the supernatants were determined using DC™ Protein Assay Kit (Bio-Rad #5 000 112). Equal concentrations of protein (30 μg ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized from total purified RNA (1 μg) from each sample by using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 0,5 %) and protein amount was measured using a Lowry based method (DC Protein Assay Kit 5000111, Bio-Rad). 10μg protein per sample were subjected for protein digestion and peptide purification using the iST label free sample preparation kit (PreOmics GmbH) ...
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bioRxiv - Genomics 2022Quote: ... After incubation with HRP-coupled secondary antibody diluted in saturation buffer (1 h, room temperature), membranes were revelated using ECL kit (Pierce, 34076) on Chemidoc XRS+ imager (Biorad). Images were processed and analyzed using ImageJ software ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of one μg of total RNA was used for reverse transcription with iScript cDNA synthesis kit (Bio-Rad). Real-time PCR was performed using SYBR Green PCR Master Mix (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... One μg of RNA was used to perform the reverse transcriptase reaction with iScript TM cDNA Synthesis Kit (Bio-Rad). Changes in mRNA levels of the target genes were determined by relative RT-qPCR with a CFX96TM Real‒Time PCR Detection System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... The spleen was removed and processed in tissue protein extraction reagent for cytokine analysis and protein quantification using a standard Bradford protein assay kit (Biorad). Subsequently tissues and serum were analyzed for cytokine levels using standard ELISA kits ...
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bioRxiv - Physiology 2023Quote: ... ∼300ng of purified RNA was revers transcribed into cDNA in the 20 μL using iScript cDNA synthesis kit (Bio-Rad). 2 μL of this reaction was used for subsequent qPCR reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed by spectrophotometry with PierceTM BCA Protein Assay Kit (ThermoFischer Scientific, cat # 23225) and proteins were separated on a 4% to 20% polyacrylamide gel (Biorad), and transferred to PVDF membrane (Bio-rad ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNA concentration was quantified by spectrophotometry and used to prepare cDNA using the iScript cDNA Synthesis Kit (Bio-RAD), followed by the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time RT-qPCR was performed using the AB StepOnePlus Detection System and the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: TNF-α and IL-10 were quantified in the collected supernatants using Bio-Plex Pro Human Luminex kit (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... and reverse transcribed by Maxima first strand cDNA synthesis kit (Thermo Fischer) using Bio-Rad CFX Maestro 1.1 software (Bio-Rad). The primers used are listed in Supplementary table 6.
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bioRxiv - Cell Biology 2024Quote: ... submerged with ECL solution (ECL Prime Kit; Amersham, UK) and chemiluminescence signals were detected using the ChemiDoc system from BioRad. The following antibodies were used ...
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bioRxiv - Molecular Biology 2024Quote: HepG2 protein lysate was separated by SDS-Page (TGX Stain-FreeTM FastCastTM Acrylamide Kit, Bio-Rad Laboratories, Inc., Hercules, US) and blotted on nitrocellulose membranes (Trans-Blot® TurboTM ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was then synthesized from 1 µg total RNA (measured by spectrophotometer) with the iScriptTM cDNA synthesis kit (Bio-RAD) according to the company’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... were transferred to a 0.45μM nitrocellulose membrane (Trans-Blot Turbo RTA Transfer kit nitrocellulose midi #1704271) using Trans-Blot Turbo Transfer System (Bio-Rad #1704150 ...
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bioRxiv - Immunology 2024Quote: ... cDNA was made from equivalent starting amounts for each sample and synthesized using the iScript cDNA Synthesis Kit (Bio-Rad). Total cDNA was diluted 1:10 in water and amplified by real-time polymerase chain reaction using a BioRad CFX-96 iCycler and SYBER Green Supermix ...