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Citations for Bio-Rad :
5601 - 5650 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were treated with an enhanced chemiluminescence kit according to the user manual and developed using a ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Cancer Biology 2022Quote: Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The protein content of the LDL sample was determined by a protein assay kit (Bio-Rad Laboratories, Inc., Tokyo, Japan) based on the Bradford method using bovine serum albumin as a standard ...
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bioRxiv - Developmental Biology 2022Quote: ... 1ug RNA was in a 20ul reaction volume to generate cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad, 1708891). Typically ...
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bioRxiv - Microbiology 2022Quote: ... we synthesized first-strand cDNA from cellular RNA isolated (100ng) from each sorted sample using iScriptTM cDNA synthesis kit (BioRad). Each rtPCR reaction included 25°C for 5 mins ...
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bioRxiv - Neuroscience 2022Quote: ... an equal amount (500 ng) of RNA was reverse transcribed to cDNA using iScript reverse-transcription kit (Bio-rad, 1708841). Quantitative real time PCR (45 cycles – LightCycler480 software ...
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bioRxiv - Microbiology 2022Quote: ... Viral copy numbers were assessed via digital-droplet PCR (ddPCR) using SARS-CoV-2 ddPCR kit (#12013743) from Bio-Rad with N1 and N2 primers and probes targeting the nucleocapsid genes ...
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bioRxiv - Microbiology 2022Quote: HCV RNA copies were determined by Taqman RT-qPCR using a Quanta BioSciences qScript XLT One-Step RT-qPCR kit according to the manufacture’s instruction (Bio-Rad). GLT1_probe was used to detect for GLT1 RNA ...
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bioRxiv - Cell Biology 2022Quote: ... following the manufactures instructions and cDNA was synthesized from 1μg of total RNA using iScript cDNA Synthesis Kit (Bio-Rad). QPCR was performed on a CFX96 real-time PCR thermocycler (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Up to 1 µg of total RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) or gDNA Clear iScript cDNA Synthesis Kit for patient-derived tumor cells (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: ... The blots were developed with an enhanced chemiluminescence kit (Thermo Fischer Scientific) and signals acquired by a ChemiDoc instrument (BioRad). All primary histone variant-specific and H3 marks-specific antibodies were used at 1 µg/ml dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit (Bio-Rad) in the Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of whole protein from each sample was subjected to SDS-PAGE using the TGX Stain-Free FastCast Acrylamide kit (BioRad). Protein bands were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was quantified via a one-step qRT-PCR using the iTaq universal probes one-step kit (Bio-Rad) per the manufacturer’s instructions for a 20µL reaction ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 1 μg of purified RNA using the iScript cDNA synthesis kit (Cat. no. 1708891, Bio-Rad). Quantitative real-time PCR assays were performed using the iQ SYBR Green Supermix (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Blots were developed using the SuperSignal West Pico PLUS chemiluminescent substrate kit (Thermo) and imaged using the ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... Quantification of RNA sample quality and concentration was performed using the Experion ‘StdSens’ kit (Bio-Rad Laboratories, Inc., Hercules, CA). Stranded RNA-Seq libraries were created and quantified by qPCR (2×76 bp or 2×151 bp ...
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bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µg of RNA from each sample was reverse-transcribed in 40 µl using iScript™ cDNA Synthesis Kit (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... proteins from SDS-PAGE gels were transferred onto nitrocellulose membranes using the Transblot Turbo RTA Midi Transfer kit (BioRad, USA). Mouse anti-α-tubulin antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine RNA concentrations and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 200 ng of RNA ...
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bioRxiv - Biochemistry 2021Quote: ... 500 ng of DNaseI-treated RNA was used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative RT-PCR was performed using SsoAdvanced SYBR green supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was then treated for 45 min at room temperature with the appropriated HRP-conjugated secondary antibody: Proteins were visualized using an ECL+ detection kit (Amersham) and imaged using a Chemidoc Touch Imaging system (Biorad). Densitometry was analyzed using the ImageLab software.
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bioRxiv - Cancer Biology 2022Quote: ... About 500 ng of total RNA was used for cDNA synthesis (iScript cDNA synthesis kit, Bio-Rad, Hercules, California, USA). cDNA was diluted 1:5 before use in PCR reactions ...
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bioRxiv - Molecular Biology 2022Quote: ... 500ng for males per sample) was subsequently reverse-transcribed to cDNA using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc.)
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bioRxiv - Genetics 2020Quote: ... Total RNA (12.5 ng) was reverse transcribed and amplified with the iTaq Universal SYBR Green One-Step Kit (Bio-Rad). RUNX1 and ACTB were amplified in parallel in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
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bioRxiv - Physiology 2021Quote: ... the concentration of protein in the whole homogenates was determined with the DC protein assay kit (Bio-Rad, Hercules, CA). Equal amounts of protein from each sample were dissolved in Laemmli buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized on 1μg of isolated RNA by reverse transcription with random hexamer primers using the iScript Select cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the gels were washed in ddH2O and then silver stained following the manufacturers instruction (Silver Stain Plus Kit, 1610449, Biorad).
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bioRxiv - Neuroscience 2020Quote: ... Results were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories, California, USA).
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bioRxiv - Neuroscience 2020Quote: ... Data were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories, California, USA).
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bioRxiv - Cell Biology 2021Quote: ... Gene expression analysis was conducted with 50 ng RNA using iTaq Universal One-Step RT-qPCR Kit (Bio-rad, 1725140). The reaction was carried out according to the manufacturer’s instructions using CFX Connect Real-Time PCR Detection System (Bio-rad ...
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bioRxiv - Immunology 2021Quote: ... Total protein content was measured by the Pierce BCA Protein Assay Kit (Thermo) and equivalent protein levels were loaded into an polyacrylamide gel (BioRad). Samples were transferred onto a nitrocellulose membrane using a semi-dry transfer apparatus before blocking with a 5% skim milk solution for thirty minutes at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates were clarified by centrifugation at 6,000 rpm for 7 min and protein concentration was determined using a DC protein assay kit (Bio-Rad). Samples containing equal amounts of protein were brought to a total volume of 1 ml with appropriate buffer.
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bioRxiv - Physiology 2021Quote: RNA was isolated using a Macherey-Nagel NucleoSpin RNA kit and cDNA was synthesized using iScript RT Supermix (Bio-Rad). Samples were run on a Bio-Rad CFX96 Touch to assess mRNA levels of BNP ...
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bioRxiv - Biochemistry 2021Quote: The 100µl plasma was inactivated by heating at 56°C for 30 min 10 .60µl plasma was used for albumin and globulin depletion using an Aurum serum mini kit (BioRad, USA). The six depleted plasma samples were used for SWATH-MS analysis ...
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bioRxiv - Physiology 2020Quote: ... and circulating factor concentrations were measured using the following kits according to the respective manufacturer’s protocols: Bio-Plex protein array system (Biorad, UK) with one multiplex panel was used to measure total Glucagon-like peptide-1 (GLP-1) ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred to PVDF membranes using Trans-Blot Turbo RTA Mini 0.45 mM LF PVDF Transfer Kit (Bio-Rad), and blotted with anti-HA antibody ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein transfer to the PVDF membrane was performed following manufacturer’s instructions for the membrane kit (Trans-Blot Turbo Midi 0.2 μm PVDF, 1704157, BioRad, Hercules, CA) and the transfer apparatus (Trans-Blot® Turbo™ Transfer System ...
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bioRxiv - Genetics 2021Quote: ... Immunolabelling was visualized using chemiluminescence kits (SuperSignal West Pico PLUS Chemiluminescent Substrate; ThermoScientific) on a Chemidoc MP Imaging System (Biorad) and analyzed with the ImageLab Software (version 5.2 ...
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bioRxiv - Neuroscience 2021Quote: ... A qScript cDNA Synthesis kit (QuantaBio, Beverly, MA, USA) and T100 Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) were used to generate the cDNA ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA was used for RNA-seq analysis or prepared for qRT-PCR using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was used as template in SYBR green qPCR supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: qPCR was performed using the iTaq universal SYBRGreen® kit following the manufacturer protocol (Bio-Rad Laboratories Inc, Hercules, CA) on an Agilent Mx3005P qPCR Systems (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... was added to the samples according to the manufacturer’s instruction and equivalent amounts of protein (30 μg as measured using the DC Protein Assay Kit (Bio-Rad) and proteins were separated on a 4 to 12% on a 12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA with a RIN value of at least 8 was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... 100-200 ng of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad) on an Arktik thermal cycler (Thermo scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes went through a final TBST wash and then visualized on the ChemiDoc XRS+ using the Clarity Western ECL kit (BioRad).