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Citations for Bio-Rad :
5651 - 5700 of 7483 citations for Mouse Claudin 1 CLDN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... All gene transcripts were quantified by quantitative PCR using qScriptTM One-Step qRT-PCR Kit (Quanta Biosciences, #95057-050) on CFX96 real-time PCR system (Bio-Rad). Primer sequences for qPCR were as follow ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time PCR (qPCR) was carried out by using SYBR Green Supermix kit with the iCycler QTX detection system (Bio-Rad). Primers are listed in Table EV5 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was established using BCA kits (Pierce) and 10µg of protein was loaded into each well of a 4-20% pre-cast gel (BioRad, MiniProtean TGX), flanked with ladder at either end of the gel (PageRuler ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of 1000 ng of RNA was used for cDNA synthesis using a High Capacity cDNA Reverse Transcription Kit (BioRad, #1708891). To detect the contaminated DNA we used the samples processed without the transcriptase reverse enzyme as negative controls ...
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bioRxiv - Neuroscience 2022Quote: ... we synthetized cDNA using immunoprecipitated samples from Npas1-Cre-RT and RT mice (see above for detailed procedure) using the iScript cDNA synthesis kit (Bio-Rad). cDNA was preamplified using TaqMan Gene Expression Assay system (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Three to four hundred nanograms of complementary DNA was synthesized using a reverse transcriptase complementary DNA synthesis kit (iScript, Bio-Rad). Changes in mRNA expression were measured using quantitative PCR (Biorad ...
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bioRxiv - Plant Biology 2021Quote: ... and 2 μg of the RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative RT- qPCR analysis was performed on a Bio-Rad iQ5 real-time PCR instrument with the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Measurements and analyses of secreted cytokines from a murine 35-plex kit were performed using a multiplex bead-based technology (Luminex) assay with a Bio-Plex 200 instrument (Bio-Rad) after 2-fold dilution of supernatants.
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bioRxiv - Biochemistry 2021Quote: The details of the protein purification protocol for human plasma sample are described in the Aurum serum mini kit leaflet and a detailed was followed(BioRad, USA). The sample was prepared for 2-D gel electrophoresis following passive rehydration for 16-24 h ...
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bioRxiv - Plant Biology 2021Quote: ... Quality and quantity of RNA samples were assessed using the Bio-Rad Experion analyser and Experion RNA high-sense analysis kit (Bio-Rad). Total RNA from each biological replicate was amplified using the MessageAmp II aRNA amplification kit (Ambion ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected and the protein concentration was measured using a DC protein assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of protein (5 μg ...
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bioRxiv - Microbiology 2021Quote: ... and 30 ng of RNA from each sample was reverse transcribed using the iScript™ cDNA Synthesis Kit (1708891; Bio-Rad). Real-time PCR was performed using Applied Biosystems™ TaqMan™ Gene Expression Master Mix (4369016 ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA was prepared in a 10 μl volume containing 350 ng of plant total RNA that was reverse-transcribed with an iScriptTM cDNA Synthesis Kit using modified MMLV-derived reverse transcriptase (Bio-Rad), a blend of oligo-d(T ...
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bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA was quantified on a Bio-Rad T100 96-Well PCR Gradient Thermal Cycler with the SYBR Green kit (Bio-Rad, SsoAdvanced™ Universal SYBR® Green Supermix) ...
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bioRxiv - Molecular Biology 2022Quote: ... 20-30 μg of total protein was resolved on 8-16% TGX stain-free gels and transferred onto nitrocellulose membrane using the Trans-Blot Turbo RTA Transfer kit and the Trans-Blot Turbo Transfer system set to 7 min transfer protocol (all from Bio-Rad). Stain-free gel and blots were imaged using the Gel Doc EZ imaging system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Lysates from the ribopuromycylation assays were loaded into gels prepared using the TGX Stain-Free FastCast Acrylamide Kit (Bio-Rad, 1610183) (Fig ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then reverse transcription was performed by HiScript III RT SuperMix for qPCR kit (R323, Vazyme, Nanjing, China) by BIO-RAD iCycler(BIO-RAD ...
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bioRxiv - Systems Biology 2022Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 h of blocking in 5% milk ...
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bioRxiv - Neuroscience 2022Quote: One ng of total RNA was used for single-tube reverse transcription (RT) and ddPCR using the One-Step RT-ddPCR Advanced kit (Bio-Rad). Droplets were generated and subsequently analyzed using the QX100 system (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of total RNAs was used to generate cDNA using the iScript cDNA synthesis kit (Bio-Rad, Richmond, CA, USA). Real-time PCR was subsequently performed using the iTaq Universal SYBER Green Supermix using the primers listed in Supplemental Table 1 on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... was converted to cDNA with the C1000 Touch Thermal Cycler using the iScript cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad, 1708890), with the following modifications ...
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bioRxiv - Microbiology 2022Quote: ... about 25 ng of DNA-free total RNA for each lncRNA was reverse-transcribed using iTaq cDNA Synthesis Kit (Bio-Rad) and then analyzed with ABI 7500 Fast Real-time PCR System (Applied Biosystem ...
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bioRxiv - Plant Biology 2019Quote: ... RNA samples confirmed as free of genomic DNA were diluted to equivalent concentrations and subjected to cDNA synthesis with iScript cDNA Synthesis Kit according to the manufacturer’s instructions (BioRad, Hercules, CA). All cDNA synthesis reactions for a single time-point were performed simultaneously to decrease any inherent variability in the synthesis process ...
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bioRxiv - Genomics 2020Quote: ... Concentration and 260/280 ratios were quantified using a NanoDrop 1000 spectrophotometer before cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad). Primers were designed to span exon-exon boundaries wherever possible (Supplementary Table 2) ...
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bioRxiv - Genetics 2019Quote: ... Reverse transcription of 400ng of RNA into cDNA was completed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA) and the Veriti 96-well thermal cycler according to the manufacturer’s instructions (Applied Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... total RNA from Astyanax embryos of various stages (2-24 hpf) was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad) and amplified using the following primers:
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bioRxiv - Cancer Biology 2019Quote: ... membranes were incubated with secondary antibodies for one hour at room temperature and developed using Clarity ECL Detection Kit (Bio-Rad).
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bioRxiv - Genomics 2019Quote: For a detailed description of tagmentation protocols and buffer formulations refer to the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Genomics 2019Quote: ... Cells were collected and resuspended in OMNI Tagmentation Mix containing ATAC Tagmentation Buffer and ATAC Tagmentation Enzyme that are parts of the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad). The OMNI Tagmentation Mix was buffered with 1X PBS supplemented with 0.1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... was performed using 300 ng RNA with the CFx Connect Real-Time system with the iTaq Universal SYBR Green One Step Kit (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and was reverse transcribed to cDNA using either qScript XLT cDNA SuperMix (Quantabio) or iScript™cDNA Synthesis Kit (Bio-Rad). Samples were run on an Applied Biosystems Viia7 Real-time PCR system ...
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bioRxiv - Physiology 2019Quote: ... 1 μg of RNA was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170-8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of total RNA from all samples were transcribed into cDNA using iScript cDNA Synthesis Kits (Bio-Rad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD, USA) for sequencing analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... 500ng of RNA from clinical samples were converted to cDNA using the iScript cDNA synthesis kit from Biorad (Catalog number: 1708891). Primers that overlapped intron-exon junction were designed using the NCI Primer-blast software and are listed in Supplementary Table 13 ...
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bioRxiv - Pathology 2020Quote: ... 1 μg of total RNA was used as a template for reverse transcription assay using the iScript cDNA Synthesis Kit (Bio-Rad); cDNA was used for detecting FAM210A and GAPDH expression ...
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bioRxiv - Cell Biology 2021Quote: ... The peroxidase-conjugated secondary antibody was incubated and reacted using ECL kits (Cytiva).The signal was detected using ChemiDoc XRS + (Bio-Rad). The membrane was stripped using stripping buffer (strong ...
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bioRxiv - Microbiology 2019Quote: ... First-strand cDNA for gene expression analysis using real-time qPCR was carried out using the iScript cDNA synthesis kit from Bio-Rad following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 250 ng of RNA was used as a template in each reaction with iTaq Universal One-Step RT-qPCR Kit (Bio-Rad) or Luna Universal One-Step RT-qPCR Kit (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2020Quote: RNA extraction of positive samples of the GFP fluorescence assay as well as butanediol fermentation processes was performed using the Aurum Total RNA Mini Kit (BioRad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Reverse transcription: 200 ng of RNA was reverse transcribed using iScript Advanced cDNA Synthesis kit for RT-qPCR (1702537, Bio-Rad) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were resolved on SDS polyacrylamide gels and blotted onto PVDF membranes using Trans-Blot Turbo RTA Midi 0.45 μm LF PVDF Transfer Kit (Bio-Rad, 1704275). Transfer was run in Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... the membrane was then developed by incubation with chemiluminescence (Amersham ECL prime kit) and imaged with a ChemiDoc™ XRS+ System (BIORAD). The intensity of GFP bands were quantified with Image J software ...
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bioRxiv - Bioengineering 2021Quote: ... Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves: an iScript cDNA synthesis kit (Bio-Rad) was used to reverse transcribe total RNA into cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Austria).52 The luciferase activity data was normalised to protein content measured by DC Protein determination kit (Bio-Rad Laboratories, Inc., USA) and SCO effect was expressed as a fold-change over untreated cells ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK).
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bioRxiv - Immunology 2020Quote: ... the signals were detected using an enhanced chemiluminescence detection kit (Super Signal West Pico Plus, 34580, ThermoFischer Scientific) and membranes imaged with ChemiDoc Imaging System (Bio-Rad). The same anti-S1 and anti-S2 antibodies as well secondary antibodies were also used to detect the Spike protein on EVs by ELISA (see protocol below).
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bioRxiv - Molecular Biology 2020Quote: 500ng of genomic RNA were used for reverse transcription into cDNA using the iScript cDNA synthesis kit (Bio-Rad, #170-8891). cDNA was diluted 10X and 2uL was added for each qPCR reaction (iQ™ SYBR Green Supermix ...