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Citations for Bio-Rad :
5551 - 5600 of 7483 citations for Mouse Claudin 1 CLDN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The RNA concentration was quantified by spectrophotometry and used to prepare cDNA using the iScript cDNA Synthesis Kit (Bio-RAD), followed by the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time RT-qPCR was performed using the AB StepOnePlus Detection System and the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: The total protein concentration of the ECM digests was measured using a DC protein assay kit (Bio-Rad, Watford, UK) following the manufacturer’s instruction (n = 4) ...
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bioRxiv - Physiology 2023Quote: ... 500 ng RNA was reverse transcribed using HiScript III 1st Strand cDNA Synthesis Kit followed by qPCR reactions using SYBR Green master mix and analyzed by Biorad CFX Touch Real-Time PCR Detection System (SIA-PCR008) ...
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bioRxiv - Neuroscience 2023Quote: 500ng of RNA/sample was used to make cDNA using iScript cDNA synthesis kit according to manufacturer’s protocol (Bio-Rad). cDNA abundance was measured using 300nM of indicated primers and a iQ5 qPCR system (Bio-Rad).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... aliquots constituting 250 ng of RNA were diluted with nuclease-free water to a volume of 15 µL and processed using the iScriptTM cDNA Synthesis Kit (BioRad Laboratories Inc. ...
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bioRxiv - Genomics 2023Quote: ... Two μg of total RNA each was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Oligonucleotide primers used for RT-PCR for each gene are listed in Supplementary Table S4 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was measured using the modified Lowry assay (DCTM Protein Assay Kit, BIO-RAD, 5000111). The same amount of total protein was mixed with Laemmli sample buffer (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... leaf tissue was ground in liquid N2 and total RNA was extracted using the Aurum Total RNA Mini Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies conjugated with alkaline phosphatase were used with an ImmunStar™-Alkaline Phosphatase (AP) Western Chemiluminescent Kit (Bio-Rad) for signal development ...
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bioRxiv - Genetics 2023Quote: ... 800 ng of total RNA were used to perform the RT step using the iScript cDNA synthesis kit (Bio-Rad) in a total volume of 20 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... The total protein concentration for each supernatant was measured with a DC protein assay kit (5000113, 5000114, 5000115; Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Total RNA was reverse transcribed with oligo (dT) primers for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad). The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: HIV-1/N and M strains were quantified by targeting the long terminal repeat (LTR) region using GENERIC HIV Charge virale kit (Biocentric) performed on CFX96 Deep-Well (Biorad) with the followed program RT 10 min at 50°C and 5 min at 95°C ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA used for ddPCR was transcribed using the iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Biorad, Munich, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified with a dsDNA HS Assay kit on a Qubit fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... TX) and 750ng of RNA was reverse transcribed to generate cDNAs using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Relative transcript levels were analyzed by real-time qPCR using SYBR Green (SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cell Biology 2023Quote: ... All gels were transferred to PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kits (Bio-Rad, 1704274).
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using iScriptTM cDNA synthesis kit and qRT-PCR was performed using a CFX96 Touch Real-Time PCR Detection System (BioRad). Data were analyzed using the following equations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA (1μg) was reverse transcribed into complementary DNA using an iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs were transcribed using the SP6 mMessage mMachine kit (Fisher Sci #AM1340) and purified using Biorad Microbiospin columns (Biorad #7326250). Each embryo was injected with 100 pg memGFP or 200 pg mCherry mRNA ...
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) was created for each sample from equals amounts of total RNA using the iScript cDNA synthesis kit (1708890 BioRad). Real-time PCR was performed from cDNA on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... A part of the samples was then analyzed by a BioRad Fish Barcoding Kit (Bio-Rad Laboratories Inc., Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration in the lysates was determined using the Bio-Rad Protein Assay Kit – BSA (Cat#5000002, Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... HRP is detected by a chemiluminescence assay using the kit Clarity Western ECL Substrate (Bio-Rad, Marnes-la-Coquette, France), whose light emission is captured by the camera of the device ChemiDoc Touch (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 µm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: HepG2 protein lysate was separated by SDS-Page (TGX Stain-FreeTM FastCastTM Acrylamide Kit, Bio-Rad Laboratories, Inc., Hercules, US) and blotted on nitrocellulose membranes (Trans-Blot® TurboTM ...
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bioRxiv - Cancer Biology 2024Quote: ... were transferred to a 0.45μM nitrocellulose membrane (Trans-Blot Turbo RTA Transfer kit nitrocellulose midi #1704271) using Trans-Blot Turbo Transfer System (Bio-Rad #1704150 ...
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bioRxiv - Pathology 2024Quote: ... We used 200ng of total RNA to synthesize single-stranded cDNA using iScript cDNA synthesis kit based on manufacturer’s directions (Bio-Rad). cDNA was then used for qRT-PCR analysis using SYBR Green qPCR master mix (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 1µg of DNase I treated RNA was converted into cDNA using an iScript cDNA synthesis kit (BIO-RAD, Cat:1708891) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Detection was performed using ECL Advance Western Blotting Detection Kit (Amersham) and the chemiluminescence was detected using an ChemiDoc Imaging System (BioRad).
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bioRxiv - Microbiology 2024Quote: Cytokine/chemokine expression levels were further confirmed using the Bio-Plex Multi-Plex Assay Kit (Bio-Rad Laboratories, Hercules, CA). Supernatants from organ homogenates were used to determine presence of 22 proteins including eotaxin ...
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bioRxiv - Immunology 2024Quote: The concentration of total protein in each processed sample was quantified using the DC Protein Assay Kit II (Bio-Rad) with a standard curve (R2 > 0.99 ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was detected using the Clarity Western ECL kit following manufacturer protocol and imaged using a ChemiDoc XRS+ apparatus (BioRad). Pixel density for each dot was quantified using the volume tool in ImageLabTM software (BioRad).
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bioRxiv - Pathology 2024Quote: ... Total reaction volume was 10 µl and contained 5 µl of iTaq Universal SYBR green one-step kit (Bio-Rad), 300 nM of forward and reverse primers (Table S5 ...
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bioRxiv - Pathology 2024Quote: ... Hrd1 and GAPDH was quantified through one-step RT-qPCR using iTaq Universal SYBR green one-step kit (Bio-Rad) with the corresponding primiers (Table S5 ...
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bioRxiv - Microbiology 2024Quote: TNF-α and IL-10 were quantified in the collected supernatants using Bio-Plex Pro Human Luminex kit (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... and reverse transcribed by Maxima first strand cDNA synthesis kit (Thermo Fischer) using Bio-Rad CFX Maestro 1.1 software (Bio-Rad). The primers used are listed in Supplementary table 6.
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bioRxiv - Molecular Biology 2024Quote: ... Gene-specific cDNA synthesis was carried out with 2 µg of mtRNA using the iScript Select cDNA Synthesis Kit (BioRad) and oligo 2278 (see Supplementary Table ST2) ...
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bioRxiv - Genomics 2024Quote: ... 500 ng of RNA was further reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad, CA, USA). Semi-quantitative PCR was carried out using specific primers for the following genes ...
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bioRxiv - Neuroscience 2024Quote: ... and the RNA was reverse transcribed into cDNA using the iScript™ gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad), as per the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was converted to cDNA using iScript advanced cDNA synthesis kit for RT-qPCR (Cat# 1725037, Biorad) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... submerged with ECL solution (ECL Prime Kit; Amersham, UK) and chemiluminescence signals were detected using the ChemiDoc system from BioRad. The following antibodies were used ...
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bioRxiv - Immunology 2024Quote: ... cDNA was made from equivalent starting amounts for each sample and synthesized using the iScript cDNA Synthesis Kit (Bio-Rad). Total cDNA was diluted 1:10 in water and amplified by real-time polymerase chain reaction using a BioRad CFX-96 iCycler and SYBER Green Supermix ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA was reverse transcribed to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Biorad, Hercules, California) in a 20 μL reaction volume ...
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bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... and NuPAGE sample buffer and reducing buffer (LifeTechnologies) were added after measuring and adjusting the samples for protein concentration (DC protein assay kit II, Bio-Rad). 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies ...
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bioRxiv - Cell Biology 2019Quote: RNA was extracted from 40 w1118 or de2f1b 80-85 hr AEL salivary glands using the Aurum Total RNA Mini Kit (Bio-Rad) with treatment of DNase I to remove genomic DNA contamination ...
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bioRxiv - Genetics 2021Quote: ... qPCR was carried out using BioMaster HS-qPCR SYBR Blue (2×) reagent kit (Biolabmix; https://biolabmix.ru/en/) and CFX96™ Real-Time PCR Detection System (Bio-Rad). We used the following thermal cycling conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total RNA and oligo (dT) primers were reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...