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Citations for Bio-Rad :
5651 - 5700 of 7813 citations for Cortisone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... was added to the samples according to the manufacturer’s instruction and equivalent amounts of protein (30 μg as measured using the DC Protein Assay Kit (Bio-Rad) and proteins were separated on a 4 to 12% on a 12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: Thymi isolated from Foxp3hCD2 x Rag1GFP reporter mice were homogenized and lysed using Bio-Plex Cell Lysis Kit (BioRad Laboratories), and the protein content of the thymic lysates was measured using Pierce™ BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA with a RIN value of at least 8 was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... 100-200 ng of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad) on an Arktik thermal cycler (Thermo scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes went through a final TBST wash and then visualized on the ChemiDoc XRS+ using the Clarity Western ECL kit (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... elegans cDNA was amplified from extracted mRNA from wild type worms using the iScript Select cDNA Synthesis kit (Bio-Rad). KLP-18 cDNA was amplified from whole worm cDNA with gene specific primers and Q5 DNA polymerase ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA was reverse transcribed with oligo(dT) primers for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad). The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufacturer’s instruction and measured using the Eon High Performance Microplate Spectrophotometer (BioTek ...
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bioRxiv - Microbiology 2022Quote: The secretome samples were fractionated by one-dimensional SDS-PAGE with the Mini-protean® 3 kit (Bio-Rad, USA) using a 12% resolving gel and a 5% stacking gel at 200 V for 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and cDNA amplification were done using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, Hercules, CA) and a CFX96 real-time system (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... according to manufacturer’s instructions and 200 ng RNA was used for cDNA synthesis using the iScript cDNA Synthesis kit (Bio-Rad). Quantitative real-time PCR was performed in technical triplicates using Power SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... viable cells were enriched using a dead cell removal magnetic kit (130-090-101, Miltenyi Biotech) and cellular viability was assessed using Trypan blue staining (0.4%; 1450013, Bio-Rad) and an automated cell counter (TC20 ...
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bioRxiv - Cell Biology 2022Quote: ... and the resulting supernatants were normalized by total protein concentration measured by the RC DC Protein Assay kit (Bio-Rad). Equal volumes of retinal lysate containing 10 μg total protein from each mouse genotype were subjected to SDS/PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... One half microgram of total RNA was reverse transcribed into cDNA using an iScript cDNA Synthesis kit (Bio-Rad, 1708891). All TaqMan primers and probes were obtained from Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The agarose gel plugs were prepared and processed with the CHEF Mammalian Genomic DNA Plug Kit (BioRad, Cat. No. #1703591). Total RNAs used to construct RNA-seq libraries were extracted from various tissues of a female juvenile zebra shark (total length ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from kidney and cells using Aurum TM Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad) or RNAqueous™ Micro Total RNA Isolation Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... One µg of total RNA was used for first-strand cDNA synthesis with the iScript cDNA Synthesis Kit (Bio-Rad) and diluted 8 times afterwards ...
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bioRxiv - Microbiology 2022Quote: ... in 1x TBS buffer for 30 minutes at 250 V and transferred using the Trans-Blot turbo Mini 0.2 μm PDVF transfer kit (Bio-Rad) with a Transblot Turbo transfer system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 50 μL of 1X PBS and protein concentration was using The DC Protein Assay Kit (Biorad).
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bioRxiv - Microbiology 2022Quote: ... on cDNA samples using the LightCycler 480 SYBR Green I Master kit in the CFX384 RT-PCR Detection System (Biorad). The identities of the resulting amplicons were checked by melting-curve analysis using the CFX384 software ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng DNase-treated RNA was used as template for cDNA synthesis using the iScript cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad). The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... The cDNA utilized in the PCR experiments was generated via the iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules California #1708890). PCR Reactions were then performed in triplicate (3n ...
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bioRxiv - Neuroscience 2023Quote: ... a cDNA library was generated using 500 ng of total RNA using the iScript cDNA synthesis kit from Bio-Rad. The concentration of the cDNA was then quantified using the Qubit 2.0 assay for oligos per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were hybridised with an appropriate DNA probe— as indicated in figure legends and Table S2—radiolabelled with P32 prepared via random priming using the High Prime kit (BioRad). Pre-hybridisation solution was discarded ...
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bioRxiv - Physiology 2023Quote: ... was reverse transcribed and converted to complementary DNA (cDNA) using the iScript cDNA synthesis kit (1708891, Bio-Rad, Hercules, CA). Each cDNA sample was amplified with messenger RNA (mRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription of up to 1μg of total RNA was performed using the iScript cDNA Synthesis Kit (Bio-Rad, #1725038).
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bioRxiv - Cancer Biology 2024Quote: ... RNA was reverse transcribed to generate cDNA using the iScript Reverse Transcriptions SuperMix for RT-qPCR kit (Bio-Rad Laboratories). Real-time PCR was performed using the SYBR select Master Mix for CFX (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a PVDF membrane using the Bio-Rad Turbo Transblot System and transfer kit (Bio-Rad Laboratories) and fixed in methanol prior to blocking in 5% non-fat milk in TNT buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Conversion of one µg of RNA into cDNA was performed using the iScript cDNA Synthesis Kit (1708890; Bio-Rad Laboratories) and Master Cycler Pro Device (EPPE6324000.516 ...
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bioRxiv - Neuroscience 2022Quote: ... Peripheral plasma cytokine levels were determined using Bio-Rad Pro mouse cytokine multiplex commercial kits (Bio-Rad, Montreal, QC, Canada). Also ...
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bioRxiv - Cell Biology 2022Quote: ... First strand cDNA was reverse transcribed from equal amounts of total RNA using iScript cDNA synthesis kit (Biorad, Hercules, CA). Specific primers for amplification of the mouse Ctnna3 message ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: cDNA synthesis was performed on extracted mRNA as described in (Mohan et al., 2019) or according to manufacturer’s protocol using the iScript cDNA Synthesis Kit (Bio-Rad). SYBR qPCR was performed as described in (Mohan et al. ...
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bioRxiv - Cell Biology 2022Quote: ... iScript Advanced cDNA Synthesis Kit (Cat# 172-5037) and ddPCR Supermix for Probes (186-3026) were obtained from Bio-Rad. PCR primers listed in table S3 were obtained from Integrated DNA Technologies.
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 500 ng of total RNA using the BIORAD iScript cDNA cDNA synthesis kit (BIORAD, CAT#1708891), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 1000 ng of RNA was used for reverse transcription in a 6.5 µl reaction using the iTaq Universal SuperMix Two-step kit (Biorad, 1725130). 3 µl of the master mix for each probe and 2ul of the generated cDNA was pipetted into a 384-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was transcribed into cDNA using the “iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR” (Bio-Rad, Hercules, CA, USA). The real-time PCR was performed with the “SsoAdvancedTM Universal SYBR Green Supermix” (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... and 100ng of RNA was used with iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad, CA, USA) to perform a one-step RT-qPCR in a volume of 20 μl using a QuantStudio3 real-time PCR instrument from ThermoFisher (Waltham ...
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bioRxiv - Microbiology 2024Quote: ... Transfer was done with the Transblot Turbo RTA transfer kit Nitrocellulose and detection was performed with the Clarity Max Western substrate (Biorad) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA samples from monotreme tissues were reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). RNA was stored in nuclease free water at -80°C.
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were assessed using the Protein Assay BCA kit (Nacalai Tesque) with bovine serum albumin (Bio-Rad, Hercules, CA) as a standard ...
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bioRxiv - Immunology 2024Quote: ... RNA from 14 3h PBF guts was transcribed into cDNA with the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad) and RT-PCR was performed with RedTaq Polymerase (VWR ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 250-1000 ng of total RNA using an iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). Quantitative PCR for each candidate gene was performed using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs were transcribed using the SP6 mMessage mMachine kit (Fisher Sci #AM1340) and purified using Biorad Microbiospin columns (Biorad #7326250). Each embryo was injected with 100 pg memGFP or 200 pg mCherry mRNA ...
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bioRxiv - Cell Biology 2023Quote: ... All gels were transferred to PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kits (Bio-Rad, 1704274).
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bioRxiv - Genomics 2023Quote: ... Total RNA was reverse transcribed with oligo (dT) primers for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad). The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: ... Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... Proteins were resolved on 4-15% SDS-PAGE gels and transferred to nitrocellulose membrane (Trans-Blot Turbo RTA transfer kit, #1704271, Biorad). Membranes were blocked for 1h at room temperature in 5%BSA-TBS-T (TBS-0.1%Tween 20 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were resolved on 4-15% SDS-PAGE gels and transferred to nitrocellulose membrane (Trans-Blot Turbo RTA transfer kit, #1704271, Biorad). Membranes were blocked for 1h at room temperature in 5% BSA-TBS-T (TBS-0.1%Tween 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... transferred to nitrocellulose membranes using semi-dry transfer apparatus and Tans-Blot Turbo RTA Midi Nitrocellulose Transfer kit (Bio-Rad). The membranes were then blocked with 3% milk in TBST ...