Labshake search
Citations for Bio-Rad :
5551 - 5600 of 7813 citations for Cortisone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from 65 −175 ng of total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Target-specific primers designed with the assistance of the free web-based PrimerQuest® Tool (IDT ...
-
bioRxiv - Immunology 2022Quote: ... Complementary DNA (cDNA) was synthesized from 500 ng of purified RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad) following protocols provided by the manufacturer.
-
bioRxiv - Neuroscience 2020Quote: ... Samples were prepared and analyzed using a Bio-Plex Pro Mouse Cytokine 23-Plex Assay kit (Bio-Rad, Hercules, CA). Assays were performed according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2019Quote: ... RT-PCR reactions were carried out using the Bio-Rad One-Step RT-ddPCR Advanced Kit for Probes (BioRad, 1864021) following the manufacturer’s protocol with the following reaction conditions ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein content was measured using the BCA kit and 50 micrograms of protein per sample were loaded on a Bio Dot apparatus (Biorad) equipped with a 0.2 micron pore size nitrocellulose membrane soaked in buffer B (10 mM Tris-HCL pH 8.0 ...
-
bioRxiv - Genomics 2019Quote: ... The exact molarity of the pools were assess by qPCR using the KAPA Library Quantification Kit Illumina on CFX96 system (Biorad). Then each pool was sequenced on 1 flowcell of HiSeq 2000 system (paired-end ...
-
bioRxiv - Molecular Biology 2019Quote: ... Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce) and imaged and quantified with a ChemiDoc MP Imaging System (BioRad). For quantification of Cas9 protein in dCas9-KRAB and dCas9-VPR parental clones ...
-
bioRxiv - Microbiology 2019Quote: ... The antigen-antibody reaction was analyzed by using Opti-4CN detection kit according to the instructions of the manufacturer (Biorad).
-
bioRxiv - Physiology 2020Quote: ... The integrity of the extracted RNA samples was additionally determined by using an Experion RNA StdSens Analysis Kit (Bio-Rad). Any samples showing poor RNA quality were also excluded from further analysis ...
-
bioRxiv - Neuroscience 2020Quote: cDNA was synthesized from total RNA isolated from LCLs by using a reverse transcription kit iScriptTM cDNA synthesis (1708891, Biorad). The reaction was performed according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... The quality of the resulting RNA library was assessed using the Experion RNA Analysis Kit (Bio-Rad; Hercules, CA, USA), and only the samples with an integrity number 8-10 were selected for transcriptomic analysis ...
-
bioRxiv - Physiology 2020Quote: ... Protein concentration was determined in triplicate with a commercial colorimetric assay (Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia), against bovine serum albumin standards (BSA ...
-
bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System RTA kit following the manufacturer’s protocols (Bio-Rad). Subsequently ...
-
bioRxiv - Molecular Biology 2019Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified using Qbit dsDNA HS Assay kit (Invitrogen) ...
-
bioRxiv - Microbiology 2019Quote: ... 1 µg total RNA was subjected to random-primed cDNA synthesis using an iScript cDNA Synthesis kit following the manufacturer’s protocol (Bio-Rad). 1 µL of the resulting cDNA was used in triplicate 25-µL qRT-PCR reactions ...
-
bioRxiv - Plant Biology 2019Quote: ... The amount of protein recovered after lysis was determined using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad), with bovine serum albumin (BSA ...
-
bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized from pre-TRAP and TRAP RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad, #170-8841). mRNA expression was measured using TaqMan Fast Advanced Master Mix (Life Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 1µg of RNA from each sample was converted to double stranded cDNA using iScript cDNA synthesis kit (Bio-Rad). RT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Immunology 2021Quote: ... Supernatants were removed at stated time points and secreted cytokine quantities were measured using Luminex Bead technology according to Bioplex protocols and cytokine Kit (Biorad).
-
bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 0.5 µg of total RNA using a RevertAid Premium Kit (Fermentas) and subjected to RT-qPCR using iTaq Universal SYBR Green Supermix (BioRad) and 10 ng of cDNA per reaction ...
-
bioRxiv - Molecular Biology 2020Quote: ... Detection of viral RNA using one-step real-time reversetranscription PCR was performed according to (Corman et al., 2020) with iTaq Universal Probes One-Step Kit (Biorad) using primers and probes against the N-gene (N_Sarbeco_F ...
-
bioRxiv - Bioengineering 2020Quote: ... PCR was performed using the SsoAdvanced universal SYBR green supermix kit (Bio-Rad, Bio-Rad CFX connect RT-PCR machine). Custom primers were used for gene amplification (Table 2 ...
-
bioRxiv - Bioengineering 2020Quote: ... PCR was performed using the SsoAdvanced universal SYBR green supermix kit (Bio-Rad, Bio-Rad CFX connect RT-PCR machine). Custom primers were used for gene amplification (Table 2 ...
-
bioRxiv - Biochemistry 2021Quote: ... Total RNA from ddHEKs and ddMEFs were harvested utilizing the Aurum Total RNA Mini kit (Bio-Rad, Hercules, CA, USA) and DNase treated on-column ...
-
bioRxiv - Neuroscience 2020Quote: ... IL-6) were quantified using a real-time PCR detection system and SsoAdvanced Universal SYBR Green Supermix kit (BIO-RAD). In brief ...
-
bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The purified RNA was quantified and an equal amount of RNA was used for cDNA preparation using iScript cDNA synthesis kit (Biorad). GAPDH was used as a housekeeping gene was used ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Mini-PROTEAN TDX Precast Protein Gels and Trans-Blot Turbo RTA Mini PVDF Transfer Kit were purchased from Bio-Rad Laboratories (Hercules ...
-
bioRxiv - Cell Biology 2021Quote: ... The concentrations of the extracts were determined using the Bio-Rad Protein assay kit (Bio-Rad Laboratories Inc. Hercules, CA). The extracts were then aliquoted into individual tubes ...
-
bioRxiv - Cell Biology 2021Quote: ... The membrane was developed by using ECL prime western blotting detection kit (Amersham) and images were captured using Versadoc (Biorad).
-
bioRxiv - Cell Biology 2021Quote: ... and the RNA was reverse transcribed into cDNA using an iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). Quantitative PCR was performed with RT2 SYBR Green qPCR Master Mix (QIAGEN ...
-
bioRxiv - Plant Biology 2021Quote: ... Up to 500 ng of RNA was used for reverse transcription using either the iScriptTM cDNA synthesis kit (Bio-Rad) or the qScript cDNA Synthesis Kit (Quantabio) ...
-
bioRxiv - Plant Biology 2020Quote: ... the soybean AATRhg1 and GmRBOHC2 (Glyma.06G162300.1) ORFs were PCR-amplified from Williams82 cDNA generated by the iScript cDNA Synthesis Kit(Bio-Rad) and KAPA HiFi polymerase (Kapa Biosystems) ...
-
bioRxiv - Microbiology 2021Quote: ... The purified total RNAs were converted to first-strand cDNAs by using a reverse transcription kit (Bio-Rad, Cat#1708891). The relative levels of HIV-1 un-spliced transcript and human MMP-2 mRNA were measured by qRT-PCR using the primers 5’AGGGACCTGAAAGCGAAAG3’ (HIV-1 un-spliced-forward ...
-
bioRxiv - Genetics 2021Quote: ... RNA quantity and quality were determined on a NanoDrop 2000c (PeqLab) and cDNA was prepared using the iScript cDNA Synthesis Kit (BioRad). To quantify RNA expression ...
-
bioRxiv - Microbiology 2021Quote: ... purified RNA (1000 ng) was converted to cDNA by using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA) following the manufacturer’s standard procedure ...
-
bioRxiv - Microbiology 2021Quote: ... and relative transcript levels were measured for each sample in triplicate by quantitative qRT-PCR using SYBR Green PCR Kit (Biorad), on a MyiQ™ Real time PCR system (Biorad) ...
-
bioRxiv - Microbiology 2020Quote: ... IL-10) responses were measured using a customized Bio-Plex Pro™ Mouse Cytokine Th1/Th2 Assay kit (Biorad, USA) with the BioPlex 200 platform according to the manufacturer’s guidelines ...
-
bioRxiv - Plant Biology 2021Quote: ... DNAse treatment were performed as in (Gigli-Bisceglia et al., 2018; Savatin et al., 2014) and cDNA synthetized with iScript cDNA Synthesis Kit (Biorad) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: cDNA synthesis and quantitative reverse transcription PCR (qRT-PCR) were performed using the iTaq Universal SYBR Green One-Step Kit (BioRad) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Complementary DNA (cDNA) from extracted RNA was obtained using the iScript™ cDNA Synthesis Kit (Bio-rad, CAT No.:1708890) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2021Quote: ... DNAase treatment and cDNA synthesis were done with the iSript gDNA clear cDNA synthesis kit (BIO-RAD, Hercules, CA, USA). Quantitative reverse transcription-polymerase chain reaction (RT-PCR ...
-
bioRxiv - Microbiology 2021Quote: ... qPCR was performed using the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad, Cat No.: 172-5150) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... The amounts of isolated mitochondria were expressed as the protein concentration using a Bio-Rad protein assay kit (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... membranes were washed three times with TBST and were developed using a chemiluminescent ECL kit (Pierce, Cat # 32106) and images were acquired using a ChemiDocTM imaging system (BioRad).
-
bioRxiv - Cell Biology 2021Quote: ... A standardized amount of RNA was transcribed into cDNA using the iScript Advanced cDNA Synthesis Kit (Bio-Rad; 170-8843), and cDNA was then amplified using the primers ...
-
bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
-
bioRxiv - Cancer Biology 2022Quote: ... The blots were treated with an enhanced chemiluminescence kit according to the user manual and developed using a ChemiDoc MP Imaging System (BioRad).
-
bioRxiv - Cancer Biology 2022Quote: Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein content of the LDL sample was determined by a protein assay kit (Bio-Rad Laboratories, Inc., Tokyo, Japan) based on the Bradford method using bovine serum albumin as a standard ...